{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T15:32:37Z","timestamp":1784561557517,"version":"3.55.0"},"reference-count":123,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T00:00:00Z","timestamp":1655164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Chemosphere"],"published-print":{"date-parts":[[2022,10]]},"DOI":"10.1016\/j.chemosphere.2022.135298","type":"journal-article","created":{"date-parts":[[2022,6,11]],"date-time":"2022-06-11T05:42:57Z","timestamp":1654926177000},"page":"135298","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":25,"special_numbering":"C","title":["Neuronal differentiation pathways and compound-induced developmental neurotoxicity in the human neural progenitor cell test (hNPT) revealed by RNA-seq"],"prefix":"10.1016","volume":"304","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3239-2227","authenticated-orcid":false,"given":"Victoria C.","family":"de Leeuw","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Conny T.M.","family":"van Oostrom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul F.K.","family":"Wackers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen L.A.","family":"Pennings","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hennie M.","family":"Hodemaekers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldert H.","family":"Piersma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen V.S.","family":"Hessel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.chemosphere.2022.135298_bib1","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.jtemb.2016.03.001","article-title":"Methylmercury and brain development: a review of recent literature","volume":"38","author":"Antunes dos Santos","year":"2016","journal-title":"J. Trace Elem. Med. Biol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib2","series-title":"User Bulletin #2 ABI PRISM 7700 Sequence Detection System","author":"Applied Biosystems","year":"2001"},{"key":"10.1016\/j.chemosphere.2022.135298_bib3","first-page":"49","article-title":"Reference compounds for alternative test methods to indicate developmental neurotoxicity (DNT) potential of chemicals: example lists & criteria for their selection & use","author":"Aschner","year":"2017","journal-title":"ALTEX"},{"key":"10.1016\/j.chemosphere.2022.135298_bib4","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.vascn.2016.06.001","article-title":"Safety pharmacology investigations on the nervous system: an industry survey","volume":"81","author":"Authier","year":"2016","journal-title":"J. Pharmacol. Toxicol. Methods"},{"key":"10.1016\/j.chemosphere.2022.135298_bib5","doi-asserted-by":"crossref","first-page":"306","DOI":"10.14573\/altex.1712081","article-title":"Workshop report recommendation on test readiness criteria for new approach methods in toxicology: exemplified for developmental neurotoxicity 1","volume":"35","author":"Bal-Price","year":"2018","journal-title":"ALTEX"},{"key":"10.1016\/j.chemosphere.2022.135298_bib6","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.taap.2018.02.008","article-title":"Strategies to improve the regulatory assessment of developmental neurotoxicity (DNT) using in vitro methods","volume":"354","author":"Bal-Price","year":"2018","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib7","doi-asserted-by":"crossref","first-page":"e82","DOI":"10.1111\/epi.13733","article-title":"Placental passage of antiepileptic drugs at delivery and neonatal outcomes","volume":"58","author":"Bank","year":"2017","journal-title":"Epilepsia"},{"key":"10.1016\/j.chemosphere.2022.135298_bib8","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1093\/toxsci\/kfm210","article-title":"Proteomic analysis of rat striatal synaptosomes during acrylamide intoxication at a low dose rate","volume":"100","author":"Barber","year":"2007","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.chemosphere.2022.135298_bib9","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1007\/s00204-016-1709-8","article-title":"Epigallocatechin gallate (EGCG) inhibits adhesion and migration of neural progenitor cells in vitro","volume":"91","author":"Barenys","year":"2016","journal-title":"Arch. Toxicol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib10","doi-asserted-by":"crossref","first-page":"a001941","DOI":"10.1101\/cshperspect.a001941","article-title":"Signaling from axon guidance receptors","volume":"2","author":"Bashaw","year":"2010","journal-title":"Cold Spring Harbor Perspect. Biol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib11","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1007\/s00204-015-1568-8","article-title":"Comparative human and rat neurospheres reveal species differences in chemical effects on neurodevelopmental key events","volume":"90","author":"Baumann","year":"2016","journal-title":"Arch. Toxicol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib12","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.reprotox.2017.05.006","article-title":"The era of 3Rs implementation in developmental and reproductive toxicity (DART) testing: current overview and future perspectives","volume":"72","author":"Beekhuijzen","year":"2017","journal-title":"Reprod. Toxicol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.fct.2020.111643","article-title":"Acrylamide exposure represses neuronal differentiation, induces cell apoptosis and promotes tau hyperphosphorylation in hESC-derived 3D cerebral organoids","volume":"144","author":"Bu","year":"2020","journal-title":"Food Chem. Toxicol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib14","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s00737-018-0906-2","article-title":"Antidepressant use in pregnancy: are we closer to consensus?","volume":"22","author":"Campagne","year":"2019","journal-title":"Arch. Womens. Ment. Health"},{"key":"10.1016\/j.chemosphere.2022.135298_bib15","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1038\/s41586-019-1338-5","article-title":"Gene expression across mammalian organ development","volume":"571","author":"Cardoso-Moreira","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.chemosphere.2022.135298_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.ntt.2020.106865","article-title":"Potential frameworks to support evaluation of mechanistic data for developmental neurotoxicity outcomes: a symposium report","volume":"78","author":"Carlson","year":"2020","journal-title":"Neurotoxicol. Teratol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib17","doi-asserted-by":"crossref","first-page":"311","DOI":"10.12965\/jer.150263","article-title":"Antidepressants modulate glycine action in rat hippocampus","volume":"11","author":"Chang","year":"2015","journal-title":"J. Exerc. Rehabil."},{"key":"10.1016\/j.chemosphere.2022.135298_bib18","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1093\/toxsci\/kfz136","article-title":"Using a multi-stage hESC model to characterize BDE-47 toxicity during neurogenesis","volume":"171","author":"Chen","year":"2019","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.chemosphere.2022.135298_bib19","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1111\/j.1741-4520.2012.00377.x","article-title":"Developmental neurotoxicity testing: a path forward","volume":"52","author":"Crofton","year":"2012","journal-title":"Congenital. Anom."},{"key":"10.1016\/j.chemosphere.2022.135298_bib20","doi-asserted-by":"crossref","first-page":"223","DOI":"10.14573\/altex.1402121","article-title":"International STakeholder NETwork (ISTNET) for creating a developmental neurotoxicity testing (DNT) roadmap for regulatory purposes","volume":"31","author":"Crofton","year":"2014","journal-title":"ALTEX"},{"issue":"1","key":"10.1016\/j.chemosphere.2022.135298_bib21","doi-asserted-by":"crossref","first-page":"89","DOI":"10.3390\/toxics3010089","article-title":"Multifactorial origin of neurodevelopmental disorders: approaches to understanding complex etiologies","volume":"3","author":"De Felice","year":"2015","journal-title":"Toxics"},{"key":"10.1016\/j.chemosphere.2022.135298_bib22","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.reprotox.2020.09.003","article-title":"An efficient neuron-astrocyte differentiation protocol from human embryonic stem cell-derived neural progenitors to assess chemical-induced developmental neurotoxicity","volume":"98","author":"de Leeuw","year":"2020","journal-title":"Reprod. Toxicol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.neuro.2019.09.014","article-title":"Differential effects of fluoxetine and venlafaxine in the neural embryonic stem cell test (ESTn) revealed by a cell lineage map","volume":"76","author":"de Leeuw","year":"2020","journal-title":"Neurotoxicology"},{"issue":"6","key":"10.1016\/j.chemosphere.2022.135298_bib24","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1089\/cell.2020.0040","article-title":"Going back and forth: episomal vector reprogramming of peripheral blood mononuclear cells to induced pluripotent stem cells and subsequent differentiation into cardiomyocytes and neuron-astrocyte Co-cultures","volume":"22","author":"de Leeuw","year":"2020","journal-title":"Cell. Reprogr."},{"key":"10.1016\/j.chemosphere.2022.135298_bib25","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.tiv.2019.04.005","article-title":"A mode-of-action ontology model for safety evaluation of chemicals: outcome of a series of workshops on repeated dose toxicity","volume":"59","author":"Desprez","year":"2019","journal-title":"Toxicol. Vitro"},{"key":"10.1016\/j.chemosphere.2022.135298_bib26","doi-asserted-by":"crossref","first-page":"9807","DOI":"10.1073\/pnas.83.24.9807","article-title":"Individual exons encode the integral membrane domains of human myelin proteolipid protein","volume":"83","author":"Diehl","year":"1986","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.chemosphere.2022.135298_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.yrtph.2022.105167","article-title":"Integration of toxicodynamic and toxicokinetic new approach methods into a weight-of-evidence analysis for pesticide developmental neurotoxicity assessment: a case-study with DL- and L-glufosinate","volume":"131","author":"Dobreniecki","year":"2022","journal-title":"Regul. Toxicol. Pharmacol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib28","series-title":"America's Children and the Environment","year":"2019"},{"key":"10.1016\/j.chemosphere.2022.135298_bib29","doi-asserted-by":"crossref","first-page":"3047","DOI":"10.1093\/bioinformatics\/btw354","article-title":"MultiQC: summarize analysis results for multiple tools and samples in a single report","volume":"32","author":"Ewels","year":"2016","journal-title":"Bioinformatics"},{"key":"10.1016\/j.chemosphere.2022.135298_bib30","series-title":"Life Sciences","first-page":"555","article-title":"Mechanisms of methylmercury-induced neurotoxicity: evidence from experimental studies","author":"Farina","year":"2011"},{"key":"10.1016\/j.chemosphere.2022.135298_bib31","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1093\/toxsci\/kfx169","article-title":"Developmental neurotoxicants disrupt activity in cortical networks on microelectrode arrays: results of screening 86 compounds during neural network formation","volume":"160","author":"Frank","year":"2017","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.chemosphere.2022.135298_bib32","doi-asserted-by":"crossref","first-page":"311","DOI":"10.14573\/altex.1701171","article-title":"OECD\/EFSA workshop on developmental neurotoxicity (DNT): the use of non-animal test methods for regulatory purposes","author":"Fritsche","year":"2017","journal-title":"ALTEX"},{"key":"10.1016\/j.chemosphere.2022.135298_bib33","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1093\/toxsci\/kfy178","article-title":"Current availability of stem cell-based in vitro methods for Developmental Neurotoxicity (DNT) testing","volume":"165","author":"Fritsche","year":"2018","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.chemosphere.2022.135298_bib34","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1093\/toxsci\/kfy175","article-title":"Development of the concept for stem cell-based developmental neurotoxicity evaluation","volume":"165","author":"Fritsche","year":"2018","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.chemosphere.2022.135298_bib35","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.taap.2018.02.004","article-title":"Consensus statement on the need for innovation, transition and implementation of developmental neurotoxicity (DNT) testing for regulatory purposes","volume":"354","author":"Fritsche","year":"2018","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib36","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.taap.2014.12.008","article-title":"Methylmercury causes neuronal cell death through the suppression of the TrkA pathway: in vitro and in vivo effects of TrkA pathway activators","volume":"282","author":"Fujimura","year":"2015","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib37","doi-asserted-by":"crossref","first-page":"2167","DOI":"10.1016\/S0140-6736(06)69665-7","article-title":"Developmental neurotoxicity of industrial chemicals","volume":"368","author":"Grandjean","year":"2006","journal-title":"Lancet"},{"key":"10.1016\/j.chemosphere.2022.135298_bib38","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/S1474-4422(13)70278-3","article-title":"Neurobehavioural effects of developmental toxicity","volume":"13","author":"Grandjean","year":"2014","journal-title":"Lancet Neurol."},{"issue":"R2","key":"10.1016\/j.chemosphere.2022.135298_bib39","doi-asserted-by":"crossref","first-page":"R219","DOI":"10.1093\/hmg\/ddz178","article-title":"Synapse diversity and synaptome architecture in human genetic disorders","volume":"28","author":"Grant","year":"2019","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.chemosphere.2022.135298_bib40","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.1038\/mp.2017.56","article-title":"A simplified protocol for differentiation of electrophysiologically mature neuronal networks from human induced pluripotent stem cells","volume":"23","author":"Gunhanlar","year":"2018","journal-title":"Mol. Psychiatr."},{"key":"10.1016\/j.chemosphere.2022.135298_bib41","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1001\/archgenpsychiatry.2011.76","article-title":"Genetic heritability and shared environmental factors among twin pairs with autism","volume":"68","author":"Hallmayer","year":"2011","journal-title":"Arch. Gen. Psychiatr."},{"key":"10.1016\/j.chemosphere.2022.135298_bib42","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.ydbio.2018.01.019","article-title":"Molecular specification of facial branchial motor neurons in vertebrates","volume":"436","author":"Han","year":"2018","journal-title":"Dev. Biol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib43","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/j.tiv.2010.10.011","article-title":"Use of high content image analysis to detect chemical-induced changes in synaptogenesis in vitro","volume":"25","author":"Harrill","year":"2011","journal-title":"Toxicol. Vitro"},{"key":"10.1016\/j.chemosphere.2022.135298_bib44","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.taap.2018.04.001","article-title":"Testing for developmental neurotoxicity using a battery of in vitro assays for key cellular events in neurodevelopment","volume":"354","author":"Harrill","year":"2018","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib45","article-title":"Development of Integrated Approaches to Testing and Assessment (IATA) case studies on developmental neurotoxicity (DNT) risk assessment","volume":"19","author":"Hern\u00e1ndez-Jerez","year":"2021","journal-title":"EFSA J."},{"key":"10.1016\/j.chemosphere.2022.135298_bib46","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1097\/EDE.0b013e3181902d15","article-title":"The rise in autism and the role of age at diagnosis","volume":"20","author":"Hertz-Picciotto","year":"2009","journal-title":"Epidemiology"},{"key":"10.1016\/j.chemosphere.2022.135298_bib47","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.taap.2018.03.013","article-title":"Design and validation of an ontology-driven animal-free testing strategy for developmental neurotoxicity testing","volume":"1","author":"Hessel","year":"2018","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib48","doi-asserted-by":"crossref","first-page":"476","DOI":"10.5966\/sctm.2015-0108","article-title":"Stem cell-derived immature human dorsal root ganglia neurons to identify peripheral neurotoxicants","volume":"5","author":"Hoelting","year":"2016","journal-title":"Stem Cells Transl. Med."},{"key":"10.1016\/j.chemosphere.2022.135298_bib49","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/0014-5793(95)00931-X","article-title":"The death domain motif found in Fas (Apo-1) and TNF receptor is present in proteins involved in apoptosis and axonal guidance","volume":"371","author":"Hofmann","year":"1995","journal-title":"FEBS Lett."},{"key":"10.1016\/j.chemosphere.2022.135298_bib50","first-page":"35","article-title":"Cord blood acrylamide levels and birth size, and interactions with genetic variants in acrylamide-metabolising genes","volume":"20","author":"Hogervorst","year":"2021","journal-title":"Environ. Heal. A Glob. Access Sci. Source"},{"key":"10.1016\/j.chemosphere.2022.135298_bib51","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/nprot.2008.211","article-title":"Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources","volume":"4","author":"Huang","year":"2009","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.chemosphere.2022.135298_bib52","doi-asserted-by":"crossref","first-page":"33","DOI":"10.3389\/fmolb.2020.00033","article-title":"Is it time to start transitioning from 2D to 3D cell culture?","volume":"7","author":"Jensen","year":"2020","journal-title":"Front. Mol. Biosci."},{"key":"10.1016\/j.chemosphere.2022.135298_bib53","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.pscychresns.2016.05.004","article-title":"Antenatal depression, treatment with selective serotonin reuptake inhibitors, and neonatal brain structure: a propensity-matched cohort study","volume":"253","author":"Jha","year":"2016","journal-title":"Psychiatry Res. Neuroimaging."},{"issue":"4","key":"10.1016\/j.chemosphere.2022.135298_bib54","doi-asserted-by":"crossref","first-page":"24","DOI":"10.3390\/jdb6040024","article-title":"The role of apoptotic signaling in axon guidance","volume":"6","author":"Kellermeyer","year":"2018","journal-title":"J. Dev. Biol."},{"issue":"9","key":"10.1016\/j.chemosphere.2022.135298_bib55","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1016\/j.molmed.2012.07.001","article-title":"Netrin-1 in the developing enteric nervous system and colorectal cancer","volume":"18","author":"Ko","year":"2012","journal-title":"Trends Mol. Med."},{"key":"10.1016\/j.chemosphere.2022.135298_bib56","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.3390\/cells9051122","article-title":"Human induced pluripotent stem cell-derived 3D-neurospheres are suitable for neurotoxicity screening","volume":"9","author":"Kobolak","year":"2020","journal-title":"Cells"},{"key":"10.1016\/j.chemosphere.2022.135298_bib57","article-title":"Long-term consequences of chronic fluoxetine exposure on the expression of myelination-related genes in the rat hippocampus","volume":"5","author":"Kroeze","year":"2016","journal-title":"Transl. Psychiatry"},{"key":"10.1016\/j.chemosphere.2022.135298_bib58","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s00204-012-0967-3","article-title":"Human embryonic stem cell-derived test systems for developmental neurotoxicity: a transcriptomics approach","volume":"87","author":"Krug","year":"2013","journal-title":"Arch. Toxicol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib59","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1004085","article-title":"PathVisio 3: an extendable pathway analysis toolbox","volume":"11","author":"Kutmon","year":"2015","journal-title":"PLoS Comput. Biol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib60","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1001\/archpsyc.60.7.720","article-title":"Effects of exposure to selective serotonin reuptake inhibitors during pregnancy on serotonergic symptoms in newborns and cord blood monoamine and prolactin concentrations","volume":"60","author":"Laine","year":"2003","journal-title":"Arch. Gen. Psychiatr."},{"key":"10.1016\/j.chemosphere.2022.135298_bib61","first-page":"362","article-title":"Integrative functional genomic analysis of human brain development and neuropsychiatric risks","volume":"80","author":"Li","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.chemosphere.2022.135298_bib62","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.neuro.2021.01.003","article-title":"Evaluation of chemical compounds that inhibit neurite outgrowth using GFP-labeled iPSC-derived human neurons","volume":"83","author":"Li","year":"2021","journal-title":"Neurotoxicology"},{"key":"10.1016\/j.chemosphere.2022.135298_bib63","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.reprotox.2021.02.006","article-title":"Does the food processing contaminant acrylamide cause developmental neurotoxicity? A review and identification of knowledge gaps","volume":"101","author":"Lindeman","year":"2021","journal-title":"Reprod. Toxicol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib64","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1289\/ehp.1205432","article-title":"Molecular mechanism of acrylamide neurotoxicity: lessons learned from organic chemistry","volume":"120","author":"LoPachin","year":"2012","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.chemosphere.2022.135298_bib65","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S0041-008X(02)00072-8","article-title":"Acrylamide axonopathy revisited","volume":"188","author":"LoPachin","year":"2003","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib66","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib67","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1124\/mol.118.112342","article-title":"Fluoxetine affects differentiation of midbrain dopaminergic neurons in vitro","volume":"94","author":"Lupu","year":"2018","journal-title":"Mol. Pharmacol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib68","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.neuro.2013.11.008","article-title":"Burst and principal components analyses of MEA data for 16 chemicals describe at least three effects classes","volume":"40","author":"Mack","year":"2014","journal-title":"Neurotoxicology"},{"key":"10.1016\/j.chemosphere.2022.135298_bib69","first-page":"1938E","article-title":"Establishment of an a priori protocol for the implementation and interpretation of an in\u2010vitro testing battery for the assessment of developmental neurotoxicity","volume":"17","author":"Masjosthusmann","year":"2020","journal-title":"EFSA Support. Publ."},{"key":"10.1016\/j.chemosphere.2022.135298_bib70","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.2005970","article-title":"CellProfiler 3.0: next-generation image processing for biology","volume":"16","author":"McQuin","year":"2018","journal-title":"PLoS Biol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib71","doi-asserted-by":"crossref","first-page":"275","DOI":"10.14573\/altex.1807041","article-title":"Animal testing and its alternatives - the most important omics is economics","volume":"35","author":"Meigs","year":"2018","journal-title":"ALTEX"},{"key":"10.1016\/j.chemosphere.2022.135298_bib72","series-title":"Encyclopedia of Environmental Health","first-page":"342","article-title":"Prenatal exposure to industrial chemicals and pesticides and effects on neurodevelopment","author":"Miodovnik","year":"2019"},{"key":"10.1016\/j.chemosphere.2022.135298_bib73","series-title":"Toxicity Testing in the 21st Century: a Vision and a Strategy, Committee on Toxicity Testing and Assessment of Environmental Agents","author":"National Research Council","year":"2007"},{"key":"10.1016\/j.chemosphere.2022.135298_bib74","doi-asserted-by":"crossref","DOI":"10.1016\/j.scr.2020.101761","article-title":"Characterization and application of electrically active neuronal networks established from human induced pluripotent stem cell-derived neural progenitor cells for neurotoxicity evaluation","volume":"45","author":"Nimtz","year":"2020","journal-title":"Stem Cell Res."},{"key":"10.1016\/j.chemosphere.2022.135298_bib75","series-title":"Venny. An Interactive Tool for Comparing Lists with Venn Diagrams","author":"Oliveros","year":"2007"},{"key":"10.1016\/j.chemosphere.2022.135298_bib76","doi-asserted-by":"crossref","DOI":"10.3389\/fncel.2019.00129","article-title":"Organs to cells and cells to organoids: the evolution of in vitro central nervous system modelling","volume":"13","author":"Pacitti","year":"2019","journal-title":"Front. Cell. Neurosci."},{"key":"10.1016\/j.chemosphere.2022.135298_bib77","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.reprotox.2020.08.002","article-title":"An analysis of the limitations and uncertainties of in vivo developmental neurotoxicity testing and assessment to identify the potential for alternative approaches","volume":"96","author":"Paparella","year":"2020","journal-title":"Reprod. Toxicol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib78","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1038\/nmeth.4197","article-title":"Salmon provides fast and bias-aware quantification of transcript expression","volume":"14","author":"Patro","year":"2017","journal-title":"Nat. Methods"},{"key":"10.1016\/j.chemosphere.2022.135298_bib79","doi-asserted-by":"crossref","first-page":"2129","DOI":"10.1093\/hmg\/11.18.2129","article-title":"Motifs within the CLN3 protein: modulation of cell growth rates and apoptosis","volume":"11","author":"Persaud-Sawin","year":"2002","journal-title":"Hum. Mol. Genet."},{"key":"10.1016\/j.chemosphere.2022.135298_bib80","first-page":"23","article-title":"Assessment of developmental neurotoxicity induced by chemical mixtures using an adverse outcome pathway concept","volume":"19","author":"Pistollato","year":"2020","journal-title":"Environ. Heal. A Glob. Access Sci. Source"},{"key":"10.1016\/j.chemosphere.2022.135298_bib81","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/978-3-030-45493-7_5","article-title":"Dysregulation of neurite outgrowth and cell migration in autism and other neurodevelopmental disorders","volume":"25","author":"Prem","year":"2020","journal-title":"Adv. Neurobiol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib82","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1289\/ehp.01109909","article-title":"Developmental neurotoxicity of chlorpyrifos modeled in vitro: comparative effects of metabolites and other cholinesterase inhibitors on DNA synthesis in PC12 and C6 cells","volume":"109","author":"Qiao","year":"2001","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.chemosphere.2022.135298_bib83","series-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2020"},{"key":"10.1016\/j.chemosphere.2022.135298_bib84","first-page":"511","article-title":"Critical periods of vulnerability for the developing nervous system: evidence from humans and animal models","volume":"108","author":"Rice","year":"2000","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.chemosphere.2022.135298_bib85","series-title":"RStudio","year":"2020"},{"key":"10.1016\/j.chemosphere.2022.135298_bib86","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.neuro.2016.02.003","article-title":"Neurite outgrowth in human induced pluripotent stem cell-derived neurons as a high-throughput screen for developmental neurotoxicity or neurotoxicity","volume":"53","author":"Ryan","year":"2016","journal-title":"Neurotoxicology"},{"key":"10.1016\/j.chemosphere.2022.135298_bib87","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1093\/toxsci\/kfy211","article-title":"International regulatory and scientific effort for improved developmental neurotoxicity testing","volume":"167","author":"Sachana","year":"2019","journal-title":"Toxicol. Sci."},{"issue":"2","key":"10.1016\/j.chemosphere.2022.135298_bib88","doi-asserted-by":"crossref","first-page":"86","DOI":"10.3390\/biology10020086","article-title":"Toward a better testing paradigm for developmental neurotoxicity: oecd efforts and regulatory considerations","volume":"10","author":"Sachana","year":"2021","journal-title":"Biology"},{"key":"10.1016\/j.chemosphere.2022.135298_bib89","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.reprotox.2021.06.006","article-title":"The potential of mechanistic information organised within the AOP framework to increase regulatory uptake of the developmental neurotoxicity (DNT) in vitro battery of assays","volume":"103","author":"Sachana","year":"2021","journal-title":"Reprod. Toxicol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib90","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.scitotenv.2019.05.270","article-title":"Effects of chlorpyrifos on cell death and cellular phenotypic specification of human neural stem cells","volume":"683","author":"Sandoval","year":"2019","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.chemosphere.2022.135298_bib91","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.tiv.2016.10.001","article-title":"Development and characterization of a human embryonic stem cell-derived 3D neural tissue model for neurotoxicity testing","volume":"38","author":"Sandstr\u00f6m","year":"2017","journal-title":"Toxicol. Vitro"},{"key":"10.1016\/j.chemosphere.2022.135298_bib92","first-page":"813","article-title":"Toxic threats to neurologic development of children","volume":"109","author":"Schettler","year":"2001","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.chemosphere.2022.135298_bib93","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.chemosphere.2022.135298_bib94","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00204-016-1805-9","article-title":"In vitro acute and developmental neurotoxicity screening: an overview of cellular platforms and high-throughput technical possibilities","volume":"91","author":"Schmidt","year":"2017","journal-title":"Arch. Toxicol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib95","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: a software Environment for integrated models of biomolecular interaction networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res."},{"key":"10.1016\/j.chemosphere.2022.135298_bib96","doi-asserted-by":"crossref","first-page":"10340","DOI":"10.1021\/bi00033a004","article-title":"Human alanyl-tRNA synthetase: conservation in evolution of catalytic Core and microhelix recognition","volume":"34","author":"Shiba","year":"1995","journal-title":"Biochemistry"},{"issue":"2","key":"10.1016\/j.chemosphere.2022.135298_bib97","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.neuron.2015.12.008","article-title":"The cellular and molecular landscapes of the developing human central nervous system","volume":"89","author":"Silbereis","year":"2016","journal-title":"Neuron"},{"key":"10.1016\/j.chemosphere.2022.135298_bib98","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.ijheh.2018.01.010","article-title":"Prenatal organophosphate insecticide exposure and infant sensory function","volume":"221","author":"Silver","year":"2018","journal-title":"Int. J. Hyg Environ. Health"},{"key":"10.1016\/j.chemosphere.2022.135298_bib99","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1093\/toxsci\/66.2.298","article-title":"Dose-response modeling of continuous endpoints","volume":"66","author":"Slob","year":"2002","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.chemosphere.2022.135298_bib100","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.ntt.2012.07.002","article-title":"Chlorpyrifos developmental neurotoxicity: interaction with glucocorticoids in PC12 cells","volume":"34","author":"Slotkin","year":"2012","journal-title":"Neurotoxicol. Teratol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib101","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/j.1875-9114.1987.tb03496.x","article-title":"Fluoxetine: a serotonin\u2010specific, second\u2010generation antidepressant","volume":"7","author":"Sommi","year":"1987","journal-title":"Pharmacother. J. Hum. Pharmacol. Drug Ther."},{"key":"10.1016\/j.chemosphere.2022.135298_bib102","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.12688\/f1000research.7563.2","article-title":"Differential analyses for RNA-seq: transcript-level estimates improve gene-level inferences [version 2; referees: 2 approved]","volume":"4","author":"Soneson","year":"2016","journal-title":"F1000Research"},{"key":"10.1016\/j.chemosphere.2022.135298_bib103","doi-asserted-by":"crossref","DOI":"10.1016\/j.comtox.2021.100206","article-title":"Probabilistic modelling of developmental neurotoxicity based on a simplified adverse outcome pathway network","volume":"21","author":"Sp\u00eenu","year":"2022","journal-title":"Comput. Toxicol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib104","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.neuron.2014.05.035","article-title":"A quantitative framework to evaluate modeling of cortical development by neural stem cells","volume":"83","author":"Stein","year":"2014","journal-title":"Neuron"},{"key":"10.1016\/j.chemosphere.2022.135298_bib105","first-page":"275","article-title":"False discovery rates: a new deal","volume":"18","author":"Stephens","year":"2017","journal-title":"Biostatistics"},{"key":"10.1016\/j.chemosphere.2022.135298_bib106","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1093\/toxsci\/kfr034","article-title":"Assessment of chemical-induced impairment of human neurite outgrowth by multiparametric live cell imaging in high-density cultures","volume":"121","author":"Stiegler","year":"2011","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.chemosphere.2022.135298_bib107","series-title":"EURL ECVAM Library of Reference Chemicals","author":"Sund","year":"2021"},{"key":"10.1016\/j.chemosphere.2022.135298_bib108","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1590\/S1516-44462009005000015","article-title":"Effects of fluoxetine and imipramine in rat fetuses treated during a critical gestational period: a macro and microscopic study","volume":"32","author":"Swerts","year":"2010","journal-title":"Rev. Bras. Psiquiatr."},{"key":"10.1016\/j.chemosphere.2022.135298_bib109","doi-asserted-by":"crossref","first-page":"D607","DOI":"10.1093\/nar\/gky1131","article-title":"STRING v11: protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets","volume":"47","author":"Szklarczyk","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.chemosphere.2022.135298_bib110","doi-asserted-by":"crossref","DOI":"10.1016\/j.neuropharm.2018.12.024","article-title":"Prenatal valproate in rodents as a tool to understand the neural underpinnings of social dysfunctions in autism spectrum disorder","volume":"159","author":"Tartaglione","year":"2019","journal-title":"Neuropharmacology"},{"key":"10.1016\/j.chemosphere.2022.135298_bib111","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.taap.2012.05.011","article-title":"Compound-specific effects of diverse neurodevelopmental toxicants on global gene expression in the neural embryonic stem cell test (ESTn)","volume":"262","author":"Theunissen","year":"2012","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib112","doi-asserted-by":"crossref","first-page":"4173","DOI":"10.1073\/pnas.0738063100","article-title":"The netrin-1 receptors UNC5H are putative tumor suppressors controlling cell death commitment","volume":"100","author":"Thi\u00e9bault","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.chemosphere.2022.135298_bib113","doi-asserted-by":"crossref","DOI":"10.1016\/j.neuropharm.2020.108271","article-title":"Gestational exposures to organophosphorus insecticides: from acute poisoning to developmental neurotoxicity","volume":"180","author":"Todd","year":"2020","journal-title":"Neuropharmacology"},{"key":"10.1016\/j.chemosphere.2022.135298_bib114","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1111\/j.1741-4520.2012.00374.x","article-title":"Developmental neurotoxicity guideline study: issues with methodology, evaluation and regulation","volume":"52","author":"Tsuji","year":"2012","journal-title":"Congenital. Anom. (Kyoto)"},{"key":"10.1016\/j.chemosphere.2022.135298_bib115","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.neuron.2014.05.013","article-title":"CORTECON: a temporal transcriptome analysis of in vitro human cerebral cortex development from human embryonic stem cells","volume":"83","author":"van de Leemput","year":"2014","journal-title":"Neuron"},{"key":"10.1016\/j.chemosphere.2022.135298_bib116","unstructured":"Flammish Institute for Translational Research (VITO), n.d. Dashboard European Human Biomonitoring Data for visualisation of aggregated data. URL https:\/\/www.hbm4eu.eu\/what-we-do\/european-hbm-platform\/eu-hbm-dashboard\/(accessed 5.18.22)."},{"key":"10.1016\/j.chemosphere.2022.135298_bib117","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1021\/tx400402j","article-title":"Design principles of concentration-dependent transcriptome deviations in drug-exposed differentiating stem cells","volume":"27","author":"Waldmann","year":"2014","journal-title":"Chem. Res. Toxicol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib118","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1093\/toxsci\/kfy270","article-title":"Today's challenges to de-risk and predict drug safety in human \u201cmind-the-gap","volume":"167","author":"Weaver","year":"2019","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.chemosphere.2022.135298_bib119","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.reprotox.2019.09.005","article-title":"Anchoring a dynamic in vitro model of human neuronal differentiation to key processes of early brain development in vivo","volume":"91","author":"Wegner","year":"2020","journal-title":"Reprod. Toxicol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib120","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1093\/toxsci\/kfx056","article-title":"Astrocytes are protective against chlorpyrifos developmental neurotoxicity in human pluripotent stem cell-derived astrocyte-neuron cocultures","volume":"157","author":"Wu","year":"2017","journal-title":"Toxicol. Sci."},{"key":"10.1016\/j.chemosphere.2022.135298_bib121","doi-asserted-by":"crossref","first-page":"1612","DOI":"10.3389\/fimmu.2017.01612","article-title":"Transcription factor C\/EBP homologous protein in health and diseases","volume":"8","author":"Yang","year":"2017","journal-title":"Front. Immunol."},{"key":"10.1016\/j.chemosphere.2022.135298_bib122","series-title":"Human Toxicology of Chemical Mixtures","first-page":"335","article-title":"20 - autism: effect of maternal exposure to neurotoxic chemicals","author":"Zeliger","year":"2008"},{"key":"10.1016\/j.chemosphere.2022.135298_bib123","doi-asserted-by":"crossref","DOI":"10.3389\/fncel.2020.00025","article-title":"Antidepressant paroxetine exerts developmental neurotoxicity in an iPSC-derived 3D human brain model","volume":"14","author":"Zhong","year":"2020","journal-title":"Front. Cell. Neurosci."}],"container-title":["Chemosphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S004565352201791X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S004565352201791X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T12:33:41Z","timestamp":1758630821000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S004565352201791X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10]]},"references-count":123,"alternative-id":["S004565352201791X"],"URL":"https:\/\/doi.org\/10.1016\/j.chemosphere.2022.135298","relation":{},"ISSN":["0045-6535"],"issn-type":[{"value":"0045-6535","type":"print"}],"subject":[],"published":{"date-parts":[[2022,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Neuronal differentiation pathways and compound-induced developmental neurotoxicity in the human neural progenitor cell test (hNPT) revealed by RNA-seq","name":"articletitle","label":"Article Title"},{"value":"Chemosphere","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chemosphere.2022.135298","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"135298"}}