{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T17:10:53Z","timestamp":1786986653429,"version":"build-2736575974"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008702","name":"Kagoshima University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008702","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP 15H02832"],"award-info":[{"award-number":["JP 15H02832"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP 18H03392"],"award-info":[{"award-number":["JP 18H03392"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP 21H03621"],"award-info":[{"award-number":["JP 21H03621"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP 24H00782"],"award-info":[{"award-number":["JP 24H00782"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Chemosphere"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.chemosphere.2025.144767","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T04:59:04Z","timestamp":1763701144000},"page":"144767","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Three-year dynamics of methylmercury production in Hg2+-spiked paddy soils: Mercury speciation, microbial communities, and rice contamination"],"prefix":"10.1016","volume":"393","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0136-2754","authenticated-orcid":false,"given":"Hitoshi","family":"Kodamatani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Takaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shintaro","family":"Hamasuna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuyuki","family":"Ichitani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Kanzaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0557-7736","authenticated-orcid":false,"given":"Takashi","family":"Tomiyasu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.chemosphere.2025.144767_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.trac.2024.117639","article-title":"A review of sequential extraction methods for fractionation analysis of toxic metals in solid environmental matrices","volume":"173","author":"Ali","year":"2024","journal-title":"TrAC, Trends Anal. Chem."},{"key":"10.1016\/j.chemosphere.2025.144767_bib2","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/S0168-6445(03)00046-9","article-title":"Bacterial mercury resistance from atoms to ecosystems","volume":"27","author":"Barkay","year":"2003","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.chemosphere.2025.144767_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2023.132457","article-title":"The divergent effects of nitrate and ammonium application on mercury methylation , demethylation , and reduction in flooded paddy slurries","volume":"460","author":"Chen","year":"2023","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.chemosphere.2025.144767_bib4","doi-asserted-by":"crossref","first-page":"6905","DOI":"10.1002\/chem.201704840","article-title":"The Mercury problem in artisanal and small-scale gold mining","volume":"24","author":"Esdaile","year":"2018","journal-title":"Chem. Eur J."},{"key":"10.1016\/j.chemosphere.2025.144767_bib5","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.scitotenv.2008.05.040","article-title":"Mercury pollution in Guizhou, Southwestern China - an overview","volume":"400","author":"Feng","year":"2008","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.chemosphere.2025.144767_bib6","series-title":"Methylmercury Accumulation in Rice","author":"Feng","year":"2024"},{"key":"10.1016\/j.chemosphere.2025.144767_bib7","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1039\/C3EM00710C","article-title":"Mercury availability by operationally defined fractionation in granulometric distributions of soils and mine wastes from an abandoned cinnabar mine","volume":"16","author":"Fern\u00e1ndez-Mart\u00ednez","year":"2014","journal-title":"Environ. Sci. Process. Impacts"},{"key":"10.1016\/j.chemosphere.2025.144767_bib8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-024-53134-9","article-title":"Improving the efficiency of DNA extraction from iron incrustations and oilfield-produced water","volume":"14","author":"Foysal","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.chemosphere.2025.144767_bib9","doi-asserted-by":"crossref","first-page":"2715","DOI":"10.1021\/es203658f","article-title":"Dissolved organic matter enhances microbial mercury methylation under sulfidic conditions","volume":"46","author":"Graham","year":"2012","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.chemosphere.2025.144767_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2020.114323","article-title":"Determination of (Bio)-available mercury in soils: a review","volume":"263","author":"Huang","year":"2020","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.chemosphere.2025.144767_bib11","first-page":"304","article-title":"Application of modified BCR sequential extraction procedure for evaluation of heavy metal leaching in contaminated soil","volume":"64","author":"Kanjo","year":"2008","journal-title":"J. Japan Soc. Civ. Eng. Ser. G"},{"key":"10.1016\/j.chemosphere.2025.144767_bib12","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.chemosphere.2016.10.129","article-title":"An inter-laboratory comparison of different analytical methods for the determination of monomethylmercury in various soil and sediment samples: a platform for method improvement","volume":"169","author":"Kodamatani","year":"2017","journal-title":"Chemosphere"},{"key":"10.1016\/j.chemosphere.2025.144767_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2020.125827","article-title":"Detailed investigation of methylmercury accumulation in rice grain from Hg2+-spiked non-contaminated paddy field soils","volume":"247","author":"Kodamatani","year":"2020","journal-title":"Chemosphere"},{"key":"10.1016\/j.chemosphere.2025.144767_bib14","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.chemosphere.2017.01.053","article-title":"The influence of sample drying and storage conditions on methylmercury determination in soils and sediments","volume":"173","author":"Kodamatani","year":"2017","journal-title":"Chemosphere"},{"key":"10.1016\/j.chemosphere.2025.144767_bib15","doi-asserted-by":"crossref","DOI":"10.2116\/analsci.28.959","article-title":"Sensitive determination method for mercury ion, methyl-, ethyl-, and phenyl-mercury in water and biological samples using high-performance liquid chromatography with chemiluminescence detection","volume":"28","author":"Kodamatani","year":"2012","journal-title":"Anal. Sci."},{"key":"10.1016\/j.chemosphere.2025.144767_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2013.02.004","article-title":"Selective determination method for measurement of methylmercury and ethylmercury in soil\/sediment samples using high-performance liquid chromatography-chemiluminescence detection coupled with simple extraction technique","volume":"1288","author":"Kodamatani","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chemosphere.2025.144767_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2023.130983","article-title":"Amendments of nitrogen and sulfur mitigate carbon-promoting effect on microbial mercury methylation in paddy soils","volume":"448","author":"Li","year":"2023","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.chemosphere.2025.144767_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2022.128447","article-title":"Mobilization and methylation of mercury with sulfur addition in paddy soil: implications for integrated water-sulfur management in controlling Hg accumulation in rice","volume":"430","author":"Li","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.chemosphere.2025.144767_bib19","article-title":"Understanding enhanced microbial MeHg production in mining-contaminated paddy soils under sulfate amendment: changes in Hg mobility or microbial methylators?","author":"Li","year":"2019","journal-title":"Environ. Sci. Technol. acs.est.8b03511."},{"key":"10.1016\/j.chemosphere.2025.144767_bib20","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.chemosphere.2017.04.129","article-title":"Influence of sulfur on the accumulation of mercury in rice plant (Oryza sativa L.) growing in mercury contaminated soils","volume":"182","author":"Li","year":"2017","journal-title":"Chemosphere"},{"key":"10.1016\/j.chemosphere.2025.144767_bib21","doi-asserted-by":"crossref","first-page":"8149","DOI":"10.1021\/acs.est.3c02676","article-title":"Mercury and sulfur redox cycling affect methylmercury levels in rice paddy soils across a contamination gradient","volume":"57","author":"Liu","year":"2023","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.chemosphere.2025.144767_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2021.118321","article-title":"The underappreciated role of natural organic matter bond Hg(II) and nanoparticulate HgS as substrates for methylation in paddy soils across a Hg concentration gradient","volume":"292","author":"Liu","year":"2022","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.chemosphere.2025.144767_bib23","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.envint.2019.04.068","article-title":"Methylmercury production in a paddy soil and its uptake by rice plants as affected by different geochemical mercury pools","volume":"129","author":"Liu","year":"2019","journal-title":"Environ. Int."},{"key":"10.1016\/j.chemosphere.2025.144767_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2022.128752","article-title":"Uncovering geochemical fractionation of the newly deposited Hg in paddy soil using a stable isotope tracer","volume":"433","author":"Liu","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.chemosphere.2025.144767_bib25","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-019-13221-2","article-title":"Rice life cycle-based global mercury biotransport and human methylmercury exposure","volume":"10","author":"Liu","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.chemosphere.2025.144767_bib26","doi-asserted-by":"crossref","first-page":"8521","DOI":"10.1021\/acs.est.8b00647","article-title":"Quantification of mercury bioavailability for methylation using diffusive gradient in thin-film samplers","volume":"52","author":"Ndu","year":"2018","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.chemosphere.2025.144767_bib27","doi-asserted-by":"crossref","first-page":"215","DOI":"10.2131\/fts.7.215","article-title":"The dietary intake of mercury from rice and human health risk in artisanal small-scale gold mining area, Indonesia","volume":"7","author":"Novirsa","year":"2020","journal-title":"Fundam. Toxicol. Sci."},{"key":"10.1016\/j.chemosphere.2025.144767_bib28","first-page":"119","article-title":"The evaluation of mercury contamination in upland rice paddy field around artisanal small-scale gold mining area, Lebaksitu, Indonesia","volume":"10","author":"Novirsa","year":"2019","journal-title":"J. Environ. Saf."},{"key":"10.1016\/j.chemosphere.2025.144767_bib29","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/j.envint.2019.03.019","article-title":"Mercury speciation, transformation, and transportation in soils, atmospheric flux, and implications for risk management: a critical review","volume":"126","author":"O'Connor","year":"2019","journal-title":"Environ. Int."},{"key":"10.1016\/j.chemosphere.2025.144767_bib30","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1007\/s13280-017-1004-9","article-title":"A review of global environmental mercury processes in response to human and natural perturbations: changes of emissions, climate, and land use","volume":"47","author":"Obrist","year":"2018","journal-title":"Ambio"},{"key":"10.1016\/j.chemosphere.2025.144767_bib31","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1126\/science.1230667","article-title":"The genetic basis for bacterial mercury methylation","volume":"339","author":"Parks","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.chemosphere.2025.144767_bib32","doi-asserted-by":"crossref","first-page":"1670","DOI":"10.1002\/etc.4797","article-title":"Fractionation analysis of mercury in soils: a comparison of three techniques for bioavailable mercury fraction determination","volume":"39","author":"Pelcov\u00e1","year":"2020","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.chemosphere.2025.144767_bib33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1126\/sciadv.1500675","article-title":"Global prevalence and distribution of genes and microorganisms involved in mercury methylation","volume":"1","author":"Podar","year":"2015","journal-title":"Sci. Adv."},{"key":"10.1016\/j.chemosphere.2025.144767_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2024.136481","article-title":"Key active mercury methylating microorganisms and their synergistic effects on methylmercury production in paddy soils","volume":"480","author":"Pu","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.chemosphere.2025.144767_bib35","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.trac.2016.05.008","article-title":"Overview and challenges of mercury fractionation and speciation in soils","volume":"82","author":"Reis","year":"2016","journal-title":"TrAC, Trends Anal. Chem."},{"key":"10.1016\/j.chemosphere.2025.144767_bib36","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0929-1393(01)00149-4","article-title":"Extraction and purification of microbial DNA from soil and sediment samples","volume":"18","author":"Roose-Amsaleg","year":"2001","journal-title":"Appl. Soil Ecol."},{"key":"10.1016\/j.chemosphere.2025.144767_bib37","doi-asserted-by":"crossref","DOI":"10.3390\/ijerph19106290","article-title":"Mercury pollution from artisanal and small-scale gold mining in Myanmar and other Southeast Asian countries","volume":"19","author":"Soe","year":"2022","journal-title":"Int. J. Environ. Res. Publ. Health"},{"key":"10.1016\/j.chemosphere.2025.144767_bib38","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2020.01369","article-title":"Nitrospina-like bacteria are potential mercury methylators in the mesopelagic zone in the East China Sea","volume":"11","author":"Tada","year":"2020","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.chemosphere.2025.144767_bib39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s42452-020-3008-5","article-title":"Distribution of total and organic mercury in soils around an artisanal and small-scale gold mining area in West Java, Indonesia","volume":"2","author":"Tomiyasu","year":"2020","journal-title":"SN Appl. Sci."},{"key":"10.1016\/j.chemosphere.2025.144767_bib40","doi-asserted-by":"crossref","first-page":"25262","DOI":"10.1007\/s11356-019-05718-6","article-title":"Transport of mercury species by river from artisanal and small-scale gold mining in West Java, Indonesia","volume":"26","author":"Tomiyasu","year":"2019","journal-title":"Environ. Sci. Pollut. Res."},{"key":"10.1016\/j.chemosphere.2025.144767_bib41","doi-asserted-by":"crossref","DOI":"10.1016\/j.envres.2013.03.015","article-title":"The distribution of mercury around the small-scale gold mining area along the Cikaniki river, Bogor, Indonesia","volume":"125","author":"Tomiyasu","year":"2013","journal-title":"Environ. Res."},{"key":"10.1016\/j.chemosphere.2025.144767_bib42","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1021\/acs.est.3c07169","article-title":"Distribution and homogenization of multiple mercury species inputs to freshwater wetland mesocosms","volume":"58","author":"Wadle","year":"2024","journal-title":"Environ. Sci. Technol."},{"key":"10.1016\/j.chemosphere.2025.144767_bib43","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1016\/j.jhazmat.2018.11.052","article-title":"Biochar amendment to further reduce methylmercury accumulation in rice grown in selenium-amended paddy soil","volume":"365","author":"Wang","year":"2019","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.chemosphere.2025.144767_bib44","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.144892","article-title":"Significant mercury efflux from a Karst region in Southwest China - results from mass balance studies in two catchments","volume":"769","author":"Xia","year":"2021","journal-title":"Sci. Total Environ."},{"key":"10.1016\/j.chemosphere.2025.144767_bib45","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/s42773-021-00087-1","article-title":"Remediation of mercury-contaminated soils and sediments using biochar: a critical review","volume":"3","author":"Yang","year":"2021","journal-title":"Biochar"},{"key":"10.1016\/j.chemosphere.2025.144767_bib46","doi-asserted-by":"crossref","DOI":"10.1016\/j.envpol.2024.123657","article-title":"Suppression of mercury methylation in soil and methylmercury accumulation in rice by dissolved organic matter derived from sulfur-rich rape straw","volume":"346","author":"Zheng","year":"2024","journal-title":"Environ. Pollut."},{"key":"10.1016\/j.chemosphere.2025.144767_bib47","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1038\/s43016-024-00954-7","article-title":"Soil Geobacteraceae are the key predictors of neurotoxic methylmercury bioaccumulation in rice","volume":"5","author":"Zhong","year":"2024","journal-title":"Nat. Food"},{"key":"10.1016\/j.chemosphere.2025.144767_bib48","doi-asserted-by":"crossref","first-page":"197","DOI":"10.2478\/s11696-013-0436-3","article-title":"Mercury associated with size-fractionated urban particulate matter: three years of sampling in Prague, Czech Republic","volume":"68","author":"Zv\u011b\u0159ina","year":"2014","journal-title":"Chem. Pap."}],"container-title":["Chemosphere"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0045653525007155?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0045653525007155?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T09:13:46Z","timestamp":1773825226000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0045653525007155"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":48,"alternative-id":["S0045653525007155"],"URL":"https:\/\/doi.org\/10.1016\/j.chemosphere.2025.144767","relation":{},"ISSN":["0045-6535"],"issn-type":[{"value":"0045-6535","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Three-year dynamics of methylmercury production in Hg2+-spiked paddy soils: Mercury speciation, microbial communities, and rice contamination","name":"articletitle","label":"Article Title"},{"value":"Chemosphere","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chemosphere.2025.144767","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"144767"}}