{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T14:00:47Z","timestamp":1775138447055,"version":"3.50.1"},"reference-count":91,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Danish Research Council \u2018Natur og Univers\u2019; FNU"},{"name":"VILLUM Center for Bioanalytical Sciences at the University of Southern Denmark"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Chemistry and Physics of Lipids"],"published-print":{"date-parts":[[2018,7]]},"DOI":"10.1016\/j.chemphyslip.2018.03.006","type":"journal-article","created":{"date-parts":[[2018,3,23]],"date-time":"2018-03-23T22:30:46Z","timestamp":1521844246000},"page":"48-61","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":25,"special_numbering":"C","title":["Niemann-Pick C2 protein regulates sterol transport between plasma membrane and late endosomes in human fibroblasts"],"prefix":"10.1016","volume":"213","author":[{"given":"Zane","family":"Berzina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lukasz M.","family":"Solanko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed S.","family":"Mehadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Louise V.","family":"Jensen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederik W.","family":"Lund","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maciej","family":"Modzel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Szomek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarzyna A.","family":"Solanko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice","family":"Dupont","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gitte Krogh","family":"Nielsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian W.","family":"Heegaard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4963-0276","authenticated-orcid":false,"given":"Christer S.","family":"Ejsing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4995-9709","authenticated-orcid":false,"given":"Daniel","family":"W\u00fcstner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0005","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1194\/jlr.M003822","article-title":"Role of endosomal membrane lipids and NPC2 in cholesterol transfer and membrane fusion","volume":"51","author":"Abdul-Hammed","year":"2010","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0010","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1046\/j.1365-2818.1998.00347.x","article-title":"Autofluorescence of living cells","volume":"191","author":"Andersson","year":"1998","journal-title":"J. Microsc."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0015","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1515\/BC.2007.063","article-title":"Development of an assay for the intermembrane transfer of cholesterol by Niemann-Pick C2 protein","volume":"388","author":"Babalola","year":"2007","journal-title":"Biol. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0020","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.bbalip.2011.11.013","article-title":"ABCA1-dependent mobilization of lysosomal cholesterol requires functional Niemann-Pick C2 but not Niemann-Pick C1 protein","volume":"1821","author":"Boadu","year":"2012","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0025","doi-asserted-by":"crossref","first-page":"4749","DOI":"10.1091\/mbc.e04-06-0496","article-title":"Intracellular macromolecular mobility measured by fluorescence recovery after photobleaching with confocal laser scanning microscopes","volume":"15","author":"Braga","year":"2004","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0030","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1038\/msb.2012.29","article-title":"Effects of diet and development on the Drosophila lipidome","volume":"8","author":"Carvalho","year":"2012","journal-title":"Mol. Syst. Biol."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0035","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.chembiol.2015.02.007","article-title":"Quantitative analysis of proteome and lipidome dynamics reveals functional regulation of global lipid metabolism","volume":"22","author":"Casanovas","year":"2015","journal-title":"Chem. Biol."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0040","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.1126\/science.aag1417","article-title":"Lysosomal cholesterol activates mTORC1 via an SLC38A9-Niemann-Pick C1 signaling complex","volume":"355","author":"Castellano","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0045","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1194\/jlr.M002345","article-title":"MLN64 mediates egress of cholesterol from endosomes to mitochondria in the absence of functional Niemann-Pick Type C1 protein","volume":"51","author":"Charman","year":"2010","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0050","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S0006-3495(97)78645-4","article-title":"Changes in the spectral properties of a plasma membrane lipid analog during the first seconds of endocytosis in living cells","volume":"72","author":"Chen","year":"1997","journal-title":"Biophys. J."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0055","doi-asserted-by":"crossref","first-page":"31594","DOI":"10.1016\/S0021-9258(19)84073-5","article-title":"Mechanism of cholesterol transfer from the Niemann-Pick type C2 protein to model membranes supports a role in lysosomal cholesterol transport","volume":"281","author":"Cheruku","year":"2006","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0060","doi-asserted-by":"crossref","first-page":"41309","DOI":"10.1074\/jbc.M008272200","article-title":"Fate of endogenously synthesized cholesterol in Niemann-Pick type C1 cells","volume":"275","author":"Cruz","year":"2000","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0065","doi-asserted-by":"crossref","first-page":"18932","DOI":"10.1073\/pnas.1110439108","article-title":"Niemann-Pick type C 1 function requires lumenal domain residues that mediate cholesterol-dependent NPC2 binding","volume":"108","author":"Deffieu","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0070","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1083\/jcb.201004142","article-title":"A role for oxysterol-binding protein-related protein 5 in endosomal cholesterol trafficking","volume":"192","author":"Du","year":"2011","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0075","doi-asserted-by":"crossref","first-page":"5336","DOI":"10.1016\/S0021-9258(17)37692-5","article-title":"Regulation of endocytic trafficking and acidification are independent of the cystic fibrosis transmembrane regulator","volume":"269","author":"Dunn","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0080","doi-asserted-by":"crossref","first-page":"e10","DOI":"10.14440\/jbm.2014.36","article-title":"Advanced methods of microscope control using (Manager software","volume":"1","author":"Edelstein","year":"2014","journal-title":"J. Biol. Methods"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0085","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.devcel.2016.05.005","article-title":"Annexin A1 tethers membrane contact sites that mediate ER to endosome cholesterol transport","volume":"37","author":"Eden","year":"2016","journal-title":"Dev. Cell"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0090","doi-asserted-by":"crossref","first-page":"6202","DOI":"10.1021\/ac060545x","article-title":"Automated identification and quantification of glycerophospholipid molecular species by multiple precursor ion scanning","volume":"78","author":"Ejsing","year":"2006","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0095","doi-asserted-by":"crossref","first-page":"2136","DOI":"10.1073\/pnas.0811700106","article-title":"Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry","volume":"106","author":"Ejsing","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0100","doi-asserted-by":"crossref","first-page":"e0144817","DOI":"10.1371\/journal.pone.0144817","article-title":"Quantitative profiling of long-Chain bases by mass tagging and parallel reaction monitoring","volume":"10","author":"Ejsing","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0105","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1021\/ac015655c","article-title":"Quantitative profiling of phospholipids by multiple precursor ion scanning on a hybrid quadrupole time-of-flight mass spectrometer","volume":"74","author":"Ekroos","year":"2002","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0110","doi-asserted-by":"crossref","first-page":"2512","DOI":"10.1073\/pnas.0437840100","article-title":"Structure of a cholesterol-binding protein deficient in Niemann-Pick type C2 disease","volume":"100","author":"Friedland","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0115","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1111\/j.1471-4159.2010.07014.x","article-title":"Regulation of the NPC2 protein-mediated cholesterol trafficking by membrane lipids","volume":"116","author":"Gallala","year":"2011","journal-title":"J. Neurochem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0120","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1083\/jcb.128.4.549","article-title":"Evidence for nonvectorial, retrograde transferrin trafficking in the early endosomes of HEp2 cells","volume":"128","author":"Ghosh","year":"1995","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0125","doi-asserted-by":"crossref","first-page":"2177","DOI":"10.1242\/jcs.107.8.2177","article-title":"Quantification of low density lipoprotein and transferrin endocytic sorting in HEp2 cells using confocal microscopy","volume":"107","author":"Ghosh","year":"1994","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0130","doi-asserted-by":"crossref","first-page":"4983","DOI":"10.1074\/jbc.M109.037622","article-title":"Niemann-Pick C1 functions independently of Niemann-Pick C2 in the initial stage of retrograde transport of membrane-impermeable lysosomal cargo","volume":"285","author":"Goldman","year":"2010","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0135","doi-asserted-by":"crossref","first-page":"1839","DOI":"10.1111\/j.1600-0854.2008.00801.x","article-title":"BODIPY-cholesterol: a new tool to visualize sterol trafficking in living cells and organisms","volume":"9","author":"H\u00f6ltt\u00e4-Vuori","year":"2008","journal-title":"Traffic"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0140","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1074\/jbc.M108861200","article-title":"Vesicular and non-vesicular sterol transport in living cells: the endocytic recycling compartment is a major sterol storage organelle","volume":"277","author":"Hao","year":"2002","journal-title":"J. Biol. Chem."},{"issue":"June (6)","key":"10.1016\/j.chemphyslip.2018.03.006_bib0145","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1111\/tra.12163","article-title":"Ultrafast diffusion of a fluorescent cholesterol analog in compartmentalized plasma membranes","volume":"15","author":"Hiramoto-Yamaki","year":"2014","journal-title":"Traffic"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0150","doi-asserted-by":"crossref","first-page":"3135","DOI":"10.1364\/AO.15.003135","article-title":"Quantum efficiency independence of the time integrated emission from a fluorescent molecule","volume":"15","author":"Hirschfeld","year":"1976","journal-title":"Appl. Opt."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0155","doi-asserted-by":"crossref","first-page":"e79736","DOI":"10.1371\/journal.pone.0079736","article-title":"Analysis of lipid experiments (ALEX): a software framework for analysis of high-resolution shotgun lipidomics data","volume":"8","author":"Husen","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0160","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1038\/nrm2336","article-title":"Cellular cholesterol trafficking and compartmentalization","volume":"9","author":"Ikonen","year":"2008","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0165","first-page":"6","article-title":"Continuous transport of a small fraction of plasma membrane cholesterol to endoplasmic reticulum regulates total cellular cholesterol","author":"Infante","year":"2017","journal-title":"eLife"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0170","doi-asserted-by":"crossref","first-page":"15287","DOI":"10.1073\/pnas.0807328105","article-title":"NPC2 facilitates bidirectional transfer of cholesterol between NPC1 and lipid bilayers, a step in cholesterol egress from lysosomes","volume":"105","author":"Infante","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0175","doi-asserted-by":"crossref","first-page":"16769","DOI":"10.1016\/S0021-9258(18)66632-3","article-title":"Type C Niemann-Pick disease. Abnormal metabolism of low density lipoprotein in homozygous and heterozygous fibroblasts","volume":"261","author":"Kruth","year":"1986","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0180","doi-asserted-by":"crossref","first-page":"17018","DOI":"10.1074\/jbc.272.27.17018","article-title":"The fate of cholesterol exiting lysosomes","volume":"272","author":"Lange","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0185","doi-asserted-by":"crossref","first-page":"18915","DOI":"10.1074\/jbc.273.30.18915","article-title":"Circulation of cholesterol between lysosomes and the plasma membrane","volume":"273","author":"Lange","year":"1998","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0190","doi-asserted-by":"crossref","first-page":"17468","DOI":"10.1074\/jbc.M000875200","article-title":"Cholesterol movement in Niemann-Pick type C cells and in cells treated with amphiphiles","volume":"275","author":"Lange","year":"2000","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0195","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/S0022-2275(20)30161-9","article-title":"Dynamics of lysosomal cholesterol in Niemann-Pick type C and normal human fibroblasts","volume":"43","author":"Lange","year":"2002","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0200","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1111\/j.1432-1033.1997.0437a.x","article-title":"Primary structure of EPV20, a secretory glycoprotein containing a previously uncharacterized type of domain","volume":"243","author":"Larsen","year":"1997","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0205","first-page":"5","article-title":"Lysosomal membrane glycoproteins bind cholesterol and contribute to lysosomal cholesterol export","author":"Li","year":"2016","journal-title":"eLife"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0210","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1083\/jcb.108.5.1625","article-title":"The intracellular transport of low density lipoprotein-derived cholesterol is defective in Niemann-Pick type C fibroblasts","volume":"108","author":"Liscum","year":"1989","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0215","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1042\/BJ20050428","article-title":"Investigating the allosterism of acyl-CoA:cholesterol acyltransferase (ACAT) by using various sterols: in vitro and intact cell studies","volume":"391","author":"Liu","year":"2005","journal-title":"Biochem. J."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0220","doi-asserted-by":"crossref","first-page":"1840","DOI":"10.1074\/jbc.M803741200","article-title":"Characterization of fluorescent sterol binding to purified human NPC1","volume":"284","author":"Liu","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0225","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1038\/nm.1876","article-title":"Niemann-Pick disease type C1 is a sphingosine storage disease that causes deregulation of lysosomal calcium","volume":"14","author":"Lloyd-Evans","year":"2008","journal-title":"Nat. Med."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0230","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1111\/jmi.12080","article-title":"A comparison of single particle tracking and temporal image correlation spectroscopy for quantitative analysis of endosome motility","volume":"252","author":"Lund","year":"2013","journal-title":"J. Microscopy"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0235","first-page":"5","article-title":"Two-photon time-lapse microscopy of BODIPY-cholesterol reveals anomalous sterol diffusion in chinese hamster ovary cells","author":"Lund","year":"2012","journal-title":"BMC Biophys."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0240","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/2046-1682-5-20","article-title":"Two-Photon time-Lapse microscopy of BODIPY-Cholesterol reveals anomalous sterol diffusion in chinese hamster ovary cells","volume":"5","author":"Lund","year":"2012","journal-title":"BMC Biophys."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0245","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.1111\/tra.12228","article-title":"SpatTrack, an imaging toolbox for analysis of endosome motility and distribution","volume":"15","author":"Lund","year":"2014","journal-title":"Traffic"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0250","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1034\/j.1600-0854.2003.00072.x","article-title":"Recycling compartments and the internal vesicles of multivesicular bodies harbor most of the cholesterol found in the endocytic pathway","volume":"4","author":"M\u00f6bius","year":"2003","journal-title":"Traffic"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0255","doi-asserted-by":"crossref","first-page":"10207","DOI":"10.1073\/pnas.1221105110","article-title":"Abnormal accumulation and recycling of glycoproteins visualized in Niemann-Pick type C cells using the chemical reporter strategy","volume":"110","author":"Mbua","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"45","key":"10.1016\/j.chemphyslip.2018.03.006_bib0260","doi-asserted-by":"crossref","first-page":"27321","DOI":"10.1074\/jbc.M115.667469","article-title":"Multiple surface regions on the niemann-Pick C2 protein facilitate intracellular cholesterol transport","volume":"290","author":"McCauliff","year":"2015","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0265","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1007\/s11745-008-3194-1","article-title":"Fluorescence techniques using dehydroergosterol to study cholesterol trafficking","volume":"43","author":"McIntosh","year":"2008","journal-title":"Lipids"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0270","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1016\/j.bbalip.2009.03.002","article-title":"Intracellular sterol dynamics","volume":"1791","author":"Mesmin","year":"2009","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0275","doi-asserted-by":"crossref","first-page":"4004","DOI":"10.1091\/mbc.e11-04-0372","article-title":"STARD4 abundance regulates sterol transport and sensing","volume":"22","author":"Mesmin","year":"2011","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0280","series-title":"Meth. Mol. Biol.","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/978-1-4939-6875-6_10","article-title":"Synthesis and live-cell imaging of fluorescent sterols for analysis of intracellular cholesterol transport","author":"Modzel","year":"2017"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0285","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/S1387-2656(05)11007-2","article-title":"Cell and tissue autofluorescence research and diagnostic applications","volume":"11","author":"Monici","year":"2005","journal-title":"Biotechn. Ann. Rev."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0290","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.bbalip.2004.08.009","article-title":"Lipid and cholesterol trafficking in NPC","volume":"1685","author":"Mukherjee","year":"2004","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0295","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1152\/physrev.1997.77.3.759","volume":"77","author":"Mukherjee","year":"1997","journal-title":"Endocytosis. Physiol. Rev."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0300","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1083\/jcb.144.6.1271","article-title":"Endocytic sorting of lipid analogues differing solely in the chemistry of their hydrophobic tails","volume":"144","author":"Mukherjee","year":"1999","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0305","doi-asserted-by":"crossref","first-page":"9627","DOI":"10.1074\/jbc.274.14.9627","article-title":"The Niemann-Pick C1 protein resides in a vesicular compartment linked to retrograde transport of multiple lysosomal cargo","volume":"274","author":"Neufeld","year":"1999","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0310","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1083\/jcb.113.6.1267","article-title":"A novel fluorescent ceramide analogue for studying membrane traffic in animal cells: accumulation at the Golgi apparatus results in altered spectral properties of the sphingolipid precursor","volume":"113","author":"Pagano","year":"1991","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0315","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1002\/humu.10255","article-title":"Identification of 58 novel mutations in Niemann-Pick disease type C: correlation with biochemical phenotype and importance of PTC1-like domains in NPC1","volume":"22","author":"Park","year":"2003","journal-title":"Hum. Mutat."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0320","doi-asserted-by":"crossref","first-page":"8247","DOI":"10.1073\/pnas.82.23.8247","article-title":"A defect in cholesterol esterification in Niemann-Pick disease (type\u2009C) patients","volume":"82","author":"Pentchev","year":"1985","journal-title":"Proc. Nat. Acad. Sci. U. S. A."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0325","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1111\/j.1600-0854.2006.00513.x","article-title":"Sterol, protein and lipid trafficking in Chinese hamster ovary cells with Niemann-Pick type C1 defect","volume":"8","author":"Pipalia","year":"2006","journal-title":"Traffic"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0330","doi-asserted-by":"crossref","DOI":"10.1021\/acs.biochem.6b00217","article-title":"Computational analysis of sterol ligand specificity of the Niemann Pick C2 protein","author":"Poongavanam","year":"2016","journal-title":"Biochemistry"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0335","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1194\/jlr.M300009-JLR200","article-title":"Trafficking defects in endogenously synthesized cholesterol in fibroblasts, macrophages, hepatocytes, and glial cells from Niemann-Pick type C1 mice","volume":"44","author":"Reid","year":"2003","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0340","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1111\/j.1471-4159.2010.06976.x","article-title":"Niemann-Pick type C disease: molecular mechanisms and potential therapeutic approaches","volume":"116","author":"Rosenbaum","year":"2011","journal-title":"J. Neurochem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0345","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1146\/annurev-biochem-061516-044932","article-title":"Endoplasmic reticulum-plasma membrane contact sites","volume":"86","author":"Saheki","year":"2017","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0350","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/S0022-2275(20)33347-2","article-title":"Determination of cholesterol at the low picomole level by nano-electrospray ionization tandem mass spectrometry","volume":"40","author":"Sandhoff","year":"1999","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0355","doi-asserted-by":"crossref","DOI":"10.1194\/jlr.M065326","article-title":"2016. A comparative study on fluorescent cholesterol analogs as versatile cellular reporters","author":"Sezgin","year":"2016","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0360","doi-asserted-by":"crossref","first-page":"29480","DOI":"10.1074\/jbc.M110.148254","article-title":"Accessibility of cholesterol in endoplasmic reticulum membranes and activation of SREBP-2 switch abruptly at a common cholesterol threshold","volume":"285","author":"Sokolov","year":"2010","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0365","doi-asserted-by":"crossref","first-page":"2082","DOI":"10.1016\/j.bpj.2013.09.031","article-title":"Membrane orientation and lateral diffusion of BODIPY-cholesterol as a function of probe structure","volume":"105","author":"Solanko","year":"2013","journal-title":"Biophys. J."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0370","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1016\/j.bbalip.2009.02.001","article-title":"Niemann-Pick C2 (NPC2) and intracellular cholesterol trafficking","volume":"1791","author":"Storch","year":"2009","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0375","doi-asserted-by":"crossref","first-page":"2288","DOI":"10.1016\/j.bpj.2012.01.063","article-title":"A Bayesian inference scheme to extract diffusivity and potential fields from confined single-molecule trajectories","volume":"102","author":"T\u00fcrkcan","year":"2012","journal-title":"Biophys. J."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0380","doi-asserted-by":"crossref","first-page":"2153","DOI":"10.1194\/jlr.M037325","article-title":"Cholesterol-binding molecules MLN64 and ORP1L mark distinct late endosomes with transporters ABCA3 and NPC1","volume":"54","author":"van der Kant","year":"2013","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0385","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1002\/cyto.a.20593","article-title":"Chromatic aberration correction and deconvolution for UV sensitive imaging of fluorescent sterols in cytoplasmic lipid droplets","volume":"73","author":"W\u00fcstner","year":"2008","journal-title":"Cytometry Part A"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0390","doi-asserted-by":"crossref","first-page":"30325","DOI":"10.1016\/S0021-9258(18)75705-0","article-title":"Rapid nonvesicular transport of sterol between the plasma membrane domains of polarized hepatic cells","volume":"277","author":"W\u00fcstner","year":"2002","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0395","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1111\/j.1600-0854.2005.00285.x","article-title":"Direct observation of rapid internalization and intracellular transport of sterol by macrophage foam cells","volume":"6","author":"W\u00fcstner","year":"2005","journal-title":"Traffic"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0400","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1111\/j.1600-0854.2010.01040.x","article-title":"Selective visualization of fluorescent sterols in Caenorhabditis elegans by bleach-rate based image segmentation","volume":"11","author":"W\u00fcstner","year":"2010","journal-title":"Traffic"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0405","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1002\/jemt.20878","article-title":"Potential of ultraviolet widefield imaging and multiphoton microscopy for analysis of dehydroergosterol in cellular membranes","volume":"74","author":"W\u00fcstner","year":"2011","journal-title":"Microsc. Res. Technol."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0410","doi-asserted-by":"crossref","first-page":"11096","DOI":"10.3390\/molecules190811096","article-title":"Photobleaching kinetics and time-integrated emission of fluorescent probes in cellular membranes","volume":"19","author":"W\u00fcstner","year":"2014","journal-title":"Molecules"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0415","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.chemphyslip.2015.08.007","article-title":"Potential of BODIPY-cholesterol for analysis of cholesterol transport and diffusion in living cells","volume":"194","author":"W\u00fcstner","year":"2015","journal-title":"Chem. Phys. Lipids"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0420","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1091\/mbc.e06-05-0445","article-title":"Plasma membrane sterol distribution resembles the surface topography of living cells","volume":"18","author":"W\u00fcstner","year":"2007","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0425","series-title":"Cellular Lipid Metabolism","first-page":"157","article-title":"Intracellular cholesterol transport","author":"W\u00fcstner","year":"2009"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0430","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.cmet.2010.05.016","article-title":"Identification of surface residues on Niemann-Pick C2 essential for hydrophobic handoff of cholesterol to NPC1 in lysosomes","volume":"12","author":"Wang","year":"2010","journal-title":"Cell Metab."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0435","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.15252\/embj.201695917","article-title":"STARD3 mediates endoplasmic reticulum-to-endosome cholesterol transport at membrane contact sites","volume":"36","author":"Wilhelm","year":"2017","journal-title":"EMBO J."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0440","doi-asserted-by":"crossref","first-page":"17040","DOI":"10.1016\/S0021-9258(19)47337-7","article-title":"Lipoproteins activate acyl-coenzyme A:cholesterol acyltransferase in macrophages only after cellular cholesterol pools are expanded to a critical threshold level","volume":"266","author":"Xu","year":"1991","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0445","doi-asserted-by":"crossref","first-page":"11134","DOI":"10.1021\/bi801328u","article-title":"Regulation of sterol transport between membranes and NPC2","volume":"47","author":"Xu","year":"2008","journal-title":"Biochmistry"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0450","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/0092-8674(84)90414-8","article-title":"Segregation of transferrin to a mildly acidic (pH 6.5) para-Golgi compartment in the recycling pathway","volume":"37","author":"Yamashiro","year":"1984","journal-title":"Cell"},{"key":"10.1016\/j.chemphyslip.2018.03.006_bib0455","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0006-3495(82)84459-7","article-title":"Lateral mobility in membranes as detected by fluorescence recovery after photobleaching","volume":"40","author":"Yguerabide","year":"1982","journal-title":"Biophys. J."}],"container-title":["Chemistry and Physics of Lipids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009308418300094?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009308418300094?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T19:24:31Z","timestamp":1655925871000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0009308418300094"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7]]},"references-count":91,"alternative-id":["S0009308418300094"],"URL":"https:\/\/doi.org\/10.1016\/j.chemphyslip.2018.03.006","relation":{},"ISSN":["0009-3084"],"issn-type":[{"value":"0009-3084","type":"print"}],"subject":[],"published":{"date-parts":[[2018,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Niemann-Pick C2 protein regulates sterol transport between plasma membrane and late endosomes in human fibroblasts","name":"articletitle","label":"Article Title"},{"value":"Chemistry and Physics of Lipids","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chemphyslip.2018.03.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}