{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:45:58Z","timestamp":1791413158513,"version":"4.3.3"},"reference-count":34,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2015,5,1]],"date-time":"2015-05-01T00:00:00Z","timestamp":1430438400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,5,13]],"date-time":"2016-05-13T00:00:00Z","timestamp":1463097600000},"content-version":"vor","delay-in-days":378,"URL":"https:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Host & Microbe"],"published-print":{"date-parts":[[2015,5]]},"DOI":"10.1016\/j.chom.2015.04.003","type":"journal-article","created":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T20:16:43Z","timestamp":1431548203000},"page":"642-652","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":269,"title":["The Toxoplasma Dense Granule Proteins GRA17 and GRA23 Mediate the Movement of Small Molecules between the Host and the Parasitophorous Vacuole"],"prefix":"10.1016","volume":"17","author":[{"given":"Daniel\u00a0A.","family":"Gold","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron\u00a0D.","family":"Kaplan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Lis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenna\u00a0C.L.","family":"Bett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily\u00a0E.","family":"Rosowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly\u00a0M.","family":"Cirelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Bougdour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saima\u00a0M.","family":"Sidik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josh\u00a0R.","family":"Beck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Lourido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal\u00a0F.","family":"Egea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter\u00a0J.","family":"Bradley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed-Ali","family":"Hakimi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randall\u00a0L.","family":"Rasmusson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen\u00a0P.J.","family":"Saeij","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.chom.2015.04.003_bib1","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1038\/nature13574","article-title":"PTEX component HSP101 mediates export of diverse malaria effectors into host erythrocytes","volume":"511","author":"Beck","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.chom.2015.04.003_bib2","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1016\/j.chom.2013.03.002","article-title":"Host cell subversion by Toxoplasma GRA16, an exported dense granule protein that targets the host cell nucleus and alters gene expression","volume":"13","author":"Bougdour","year":"2013","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.chom.2015.04.003_bib3","doi-asserted-by":"crossref","first-page":"2071","DOI":"10.1084\/jem.20130103","article-title":"A Toxoplasma dense granule protein, GRA24, modulates the early immune response to infection by promoting a direct and sustained host p38 MAPK activation","volume":"210","author":"Braun","year":"2013","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.chom.2015.04.003_bib4","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.ijpara.2013.09.003","article-title":"Exploitation of auxotrophies and metabolic defects in Toxoplasma as therapeutic approaches","volume":"44","author":"Coppens","year":"2014","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.chom.2015.04.003_bib5","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1038\/nature08104","article-title":"A newly discovered protein export machine in malaria parasites","volume":"459","author":"de Koning-Ward","year":"2009","journal-title":"Nature"},{"key":"10.1016\/j.chom.2015.04.003_bib6","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1073\/pnas.94.5.2045","article-title":"Pore size of the malaria parasite\u2019s nutrient channel","volume":"94","author":"Desai","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chom.2015.04.003_bib7","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1111\/j.1365-2958.2010.07169.x","article-title":"Novel roles for ATP-binding cassette G transporters in lipid redistribution in Toxoplasma","volume":"76","author":"Ehrenman","year":"2010","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.chom.2015.04.003_bib8","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/0014-4827(87)90014-0","article-title":"Scrape-loading and dye transfer. A rapid and simple technique to study gap junctional intercellular communication","volume":"168","author":"el-Fouly","year":"1987","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.chom.2015.04.003_bib9","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/nature13555","article-title":"PTEX is an essential nexus for protein export in malaria parasites","volume":"511","author":"Elsworth","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.chom.2015.04.003_bib10","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1128\/EC.00357-08","article-title":"Efficient gene replacements in Toxoplasma gondii strains deficient for nonhomologous end joining","volume":"8","author":"Fox","year":"2009","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.chom.2015.04.003_bib11","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1007\/s004360050659","article-title":"Characterization of a genomic region encoding the 32-kDa dense granule antigen of Sarcocystis muris (Apicomplexa)","volume":"85","author":"Freyer","year":"1999","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.chom.2015.04.003_bib12","doi-asserted-by":"crossref","first-page":"e29998","DOI":"10.1371\/journal.pone.0029998","article-title":"Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts","volume":"7","author":"Fritz","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1016\/j.chom.2015.04.003_bib13","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1111\/j.1742-4658.2006.05123.x","article-title":"Peptides corresponding to helices 5 and 6 of Bax can independently form large lipid pores","volume":"273","author":"Garc\u00eda-S\u00e1ez","year":"2006","journal-title":"FEBS J."},{"key":"10.1016\/j.chom.2015.04.003_bib14","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1016\/j.chom.2012.09.010","article-title":"Uncovering common principles in protein export of malaria parasites","volume":"12","author":"Gr\u00fcring","year":"2012","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.chom.2015.04.003_bib15","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1038\/nrmicro2858","article-title":"Modulation of innate immunity by Toxoplasma gondii virulence effectors","volume":"10","author":"Hunter","year":"2012","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.chom.2015.04.003_bib16","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1083\/jcb.141.6.1323","article-title":"The protozoan parasite Toxoplasma gondii targets proteins to dense granules and the vacuolar space using both conserved and unusual mechanisms","volume":"141","author":"Karsten","year":"1998","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.chom.2015.04.003_bib17","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1111\/1574-6976.12013","article-title":"Subversion of host cellular functions by the apicomplexan parasites","volume":"37","author":"Kemp","year":"2013","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.chom.2015.04.003_bib18","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1128\/EC.00287-10","article-title":"Nutrient transport and pathogenesis in selected parasitic protozoa","volume":"10","author":"Landfear","year":"2011","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.chom.2015.04.003_bib19","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.parint.2013.04.003","article-title":"TgGRA23, a novel Toxoplasma gondii dense granule protein associated with the parasitophorous vacuole membrane and intravacuolar network","volume":"62","author":"Masatani","year":"2013","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.chom.2015.04.003_bib20","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1083\/jcb.98.4.1556","article-title":"A method for incorporating macromolecules into adherent cells","volume":"98","author":"McNeil","year":"1984","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.chom.2015.04.003_bib21","doi-asserted-by":"crossref","first-page":"e1003779","DOI":"10.1371\/journal.ppat.1003779","article-title":"Transcriptional analysis of murine macrophages infected with different Toxoplasma strains identifies novel regulation of host signaling pathways","volume":"9","author":"Melo","year":"2013","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.chom.2015.04.003_bib22","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1016\/j.ijpara.2005.03.011","article-title":"Dense granules: are they key organelles to help understand the parasitophorous vacuole of all apicomplexa parasites?","volume":"35","author":"Mercier","year":"2005","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.chom.2015.04.003_bib23","doi-asserted-by":"crossref","first-page":"13458","DOI":"10.1073\/pnas.1117047109","article-title":"Admixture and recombination among Toxoplasma gondii lineages explain global genome diversity","volume":"109","author":"Minot","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chom.2015.04.003_bib24","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/S1286-4579(02)01576-9","article-title":"Expression variance, biochemical and immunological properties of Toxoplasma gondii dense granule protein GRA7","volume":"4","author":"Neudeck","year":"2002","journal-title":"Microbes Infect."},{"key":"10.1016\/j.chom.2015.04.003_bib25","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.pbiomolbio.2004.01.009","article-title":"Pore-forming protein toxins: from structure to function","volume":"88","author":"Parker","year":"2005","journal-title":"Prog. Biophys. Mol. Biol."},{"key":"10.1016\/j.chom.2015.04.003_bib26","doi-asserted-by":"crossref","first-page":"17379","DOI":"10.1073\/pnas.0807764105","article-title":"Structure of transmembrane pore induced by Bax-derived peptide: evidence for lipidic pores","volume":"105","author":"Qian","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chom.2015.04.003_bib27","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1084\/jem.20100717","article-title":"Strain-specific activation of the NF-kappaB pathway by GRA15, a novel Toxoplasma gondii dense granule protein","volume":"208","author":"Rosowski","year":"2011","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.chom.2015.04.003_bib28","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1073\/pnas.91.2.509","article-title":"The parasitophorous vacuole membrane surrounding intracellular Toxoplasma gondii functions as a molecular sieve","volume":"91","author":"Schwab","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chom.2015.04.003_bib29","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01114-14","article-title":"Efficient gene disruption in diverse strains of Toxoplasma gondii using CRISPR\/CAS9","volume":"5","author":"Shen","year":"2014","journal-title":"MBio"},{"key":"10.1016\/j.chom.2015.04.003_bib30","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1111\/cmi.12293","article-title":"Identification of a new export signal in Plasmodium yoelii: identification of a new exportome","volume":"16","author":"Siau","year":"2014","journal-title":"Cell. Microbiol."},{"key":"10.1016\/j.chom.2015.04.003_bib31","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1242\/jcs.108.4.1669","article-title":"Regulated secretion of multi-lamellar vesicles leads to formation of a tubulo-vesicular network in host-cell vacuoles occupied by Toxoplasma gondii","volume":"108","author":"Sibley","year":"1995","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.chom.2015.04.003_bib32","doi-asserted-by":"crossref","first-page":"e100450","DOI":"10.1371\/journal.pone.0100450","article-title":"Efficient genome engineering of Toxoplasma gondii using CRISPR\/Cas9","volume":"9","author":"Sidik","year":"2014","journal-title":"PLoS ONE"},{"key":"10.1016\/j.chom.2015.04.003_bib33","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/0166-6851(95)02515-4","article-title":"Sporozoites of Toxoplasma gondii lack dense-granule protein GRA3 and form a unique parasitophorous vacuole","volume":"75","author":"Speer","year":"1995","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.chom.2015.04.003_bib34","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1007\/s004360050305","article-title":"Diffusion of microinjected markers across the parasitophorous vacuole membrane in cells infected with Eimeria nieschulzi (Coccidia, Apicomplexa)","volume":"83","author":"Werner-Meier","year":"1997","journal-title":"Parasitol. Res."}],"container-title":["Cell Host & Microbe"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1931312815001614?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1931312815001614?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,5,7]],"date-time":"2022-05-07T22:28:43Z","timestamp":1651962523000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1931312815001614"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5]]},"references-count":34,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["S1931312815001614"],"URL":"https:\/\/doi.org\/10.1016\/j.chom.2015.04.003","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.725495550.793506878","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.725495550.793518979","asserted-by":"object"}]},"ISSN":["1931-3128"],"issn-type":[{"value":"1931-3128","type":"print"}],"subject":[],"published":{"date-parts":[[2015,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The Toxoplasma Dense Granule Proteins GRA17 and GRA23 Mediate the Movement of Small Molecules between the Host and the Parasitophorous Vacuole","name":"articletitle","label":"Article Title"},{"value":"Cell Host & Microbe","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chom.2015.04.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}