{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T07:44:01Z","timestamp":1793087041639,"version":"4.5.0"},"reference-count":28,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T00:00:00Z","timestamp":1599782400000},"content-version":"vor","delay-in-days":376,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100006367","name":"Association Instituts Carnot","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006367","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Host & Microbe"],"published-print":{"date-parts":[[2019,9]]},"DOI":"10.1016\/j.chom.2019.08.007","type":"journal-article","created":{"date-parts":[[2019,9,3]],"date-time":"2019-09-03T10:44:00Z","timestamp":1567507440000},"page":"435-444.e4","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":198,"title":["Bioengineered Human Organ-on-Chip Reveals Intestinal Microenvironment and Mechanical Forces Impacting Shigella Infection"],"prefix":"10.1016","volume":"26","author":[{"given":"Alexandre","family":"Grassart","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Malard\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samy","family":"Gobaa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Sartori-Rupp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan","family":"Kerns","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katia","family":"Karalis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit","family":"Marteyn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Sansonetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Sauvonnet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.chom.2019.08.007_bib1","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1111\/j.1365-2958.1993.tb01097.x","article-title":"MxiD, an outer membrane protein necessary for the secretion of the Shigella flexneri lpa invasins","volume":"7","author":"Allaoui","year":"1993","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.chom.2019.08.007_bib2","doi-asserted-by":"crossref","first-page":"E3282","DOI":"10.1073\/pnas.1509091112","article-title":"BioImage analysis of Shigella infection reveals targeting of colonic crypts","volume":"112","author":"Arena","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chom.2019.08.007_bib3","doi-asserted-by":"crossref","first-page":"219","DOI":"10.3389\/fimmu.2015.00219","article-title":"Shigella manipulates host immune responses by delivering effector proteins with specific roles","volume":"6","author":"Ashida","year":"2015","journal-title":"Front. Immunol."},{"key":"10.1016\/j.chom.2019.08.007_bib4","doi-asserted-by":"crossref","first-page":"3867","DOI":"10.1073\/pnas.86.10.3867","article-title":"Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin","volume":"86","author":"Bernardini","year":"1989","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chom.2019.08.007_bib5","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.chom.2014.03.001","article-title":"IcsA is a Shigella flexneri adhesin regulated by the type III secretion system and required for pathogenesis","volume":"15","author":"Brotcke Zumsteg","year":"2014","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.chom.2019.08.007_bib6","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.chom.2014.01.005","article-title":"A fluorescent reporter reveals on\/off regulation of the Shigella type III secretion apparatus during entry and cell-to-cell spread","volume":"15","author":"Campbell-Valois","year":"2014","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.chom.2019.08.007_bib7","doi-asserted-by":"crossref","first-page":"e02567-14","DOI":"10.1128\/mBio.02567-14","article-title":"Escape of actively secreting Shigella flexneri from ATG8\/LC3-positive vacuoles formed during cell-to-cell spread is facilitated by IcsB and VirA","volume":"6","author":"Campbell-Valois","year":"2015","journal-title":"MBio"},{"key":"10.1016\/j.chom.2019.08.007_bib8","doi-asserted-by":"crossref","first-page":"C1350","DOI":"10.1152\/ajpcell.00027.2008","article-title":"Repetitive deformation activates Src-independent FAK-dependent ERK motogenic signals in human Caco-2 intestinal epithelial cells","volume":"294","author":"Chaturvedi","year":"2008","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"10.1016\/j.chom.2019.08.007_bib28","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1038\/nmeth.2075","article-title":"Icy: an open bioimage informatics platform for extended reproducible research","volume":"9","author":"de Chaumont","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.chom.2019.08.007_bib9","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1093\/infdis\/159.6.1126","article-title":"Inoculum size in shigellosis and implications for expected mode of transmission","volume":"159","author":"DuPont","year":"1989","journal-title":"J.\u00a0Infect. Dis."},{"key":"10.1016\/j.chom.2019.08.007_bib10","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.coi.2017.07.012","article-title":"Organoid culture systems to study host-pathogen interactions","volume":"48","author":"Dutta","year":"2017","journal-title":"Curr. Opin. Immunol."},{"key":"10.1016\/j.chom.2019.08.007_bib11","series-title":"Encyclopedia of cell biology","first-page":"784","article-title":"Cellular invasion by bacterial pathogens","author":"Ferrari","year":"2016"},{"key":"10.1016\/j.chom.2019.08.007_bib12","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1007\/s00204-019-02427-4","article-title":"Integrated in\u00a0vitro models for hepatic safety and metabolism: evaluation of a human liver-chip and liver spheroid","volume":"93","author":"Foster","year":"2019","journal-title":"Arch. Toxicol."},{"key":"10.1016\/j.chom.2019.08.007_bib13","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1016\/j.cellsig.2009.02.011","article-title":"The effects of mechanical forces on intestinal physiology and pathology","volume":"21","author":"Gayer","year":"2009","journal-title":"Cell. Signal."},{"key":"10.1016\/j.chom.2019.08.007_bib14","doi-asserted-by":"crossref","first-page":"1130","DOI":"10.1039\/c3ib40126j","article-title":"Gut-on-a-chip microenvironment induces human intestinal cells to undergo villus differentiation","volume":"5","author":"Kim","year":"2013","journal-title":"Integr. Biol. (Camb.)"},{"key":"10.1016\/j.chom.2019.08.007_bib15","doi-asserted-by":"crossref","first-page":"2165","DOI":"10.1039\/c2lc40074j","article-title":"Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow","volume":"12","author":"Kim","year":"2012","journal-title":"Lab Chip"},{"key":"10.1016\/j.chom.2019.08.007_bib16","first-page":"E7","article-title":"Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip","volume":"113","author":"Kim","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chom.2019.08.007_bib17","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00733-18","article-title":"Human intestinal enteroids as a model system of Shigella pathogenesis","volume":"87","author":"Koestler","year":"2019","journal-title":"Infect. Immun."},{"key":"10.1016\/j.chom.2019.08.007_bib18","doi-asserted-by":"crossref","first-page":"1488","DOI":"10.1016\/0264-410X(95)00102-7","article-title":"A modified Shigella volunteer challenge model in which the inoculum is administered with bicarbonate buffer: clinical experience and implications for Shigella infectivity","volume":"13","author":"Kotloff","year":"1995","journal-title":"Vaccine"},{"key":"10.1016\/j.chom.2019.08.007_bib19","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1038\/s41467-018-03053-x","article-title":"Mapping molecular assemblies with fluorescence microscopy and object-based spatial statistics","volume":"9","author":"Lagache","year":"2018","journal-title":"Nat. Commun."},{"key":"10.1016\/j.chom.2019.08.007_bib20","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1128\/iai.60.1.237-248.1992","article-title":"Shigella flexneri enters human colonic Caco-2 epithelial cells through the basolateral pole","volume":"60","author":"Mounier","year":"1992","journal-title":"Infect. Immun."},{"key":"10.1016\/j.chom.2019.08.007_bib21","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.mib.2008.12.002","article-title":"Shigella type III secretion effectors: how, where, when, for what purposes?","volume":"12","author":"Parsot","year":"2009","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.chom.2019.08.007_bib22","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00740-18","article-title":"Evaluating Shigella flexneri pathogenesis in the human enteroid model","volume":"87","author":"Ranganathan","year":"2019","journal-title":"Infect. Immun."},{"key":"10.1016\/j.chom.2019.08.007_bib23","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1128\/iai.35.3.852-860.1982","article-title":"Involvement of a plasmid in the invasive ability of Shigella flexneri","volume":"35","author":"Sansonetti","year":"1982","journal-title":"Infect. Immun."},{"key":"10.1016\/j.chom.2019.08.007_bib24","doi-asserted-by":"crossref","first-page":"2752","DOI":"10.1128\/iai.64.7.2752-2764.1996","article-title":"Infection of rabbit Peyer's patches by Shigella flexneri: effect of adhesive or invasive bacterial phenotypes on follicle-associated epithelium","volume":"64","author":"Sansonetti","year":"1996","journal-title":"Infect. Immun."},{"key":"10.1016\/j.chom.2019.08.007_bib27","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.chom.2019.08.007_bib25","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1016\/S0092-8674(02)00796-1","article-title":"Bacterial adhesion to target cells enhanced by shear force","volume":"109","author":"Thomas","year":"2002","journal-title":"Cell"},{"key":"10.1016\/j.chom.2019.08.007_bib26","first-page":"G250","article-title":"Quantitative assessment of villous motility","volume":"252","author":"Womack","year":"1987","journal-title":"Am. J. Physiol."}],"updated-by":[{"DOI":"10.1016\/j.chom.2019.09.007","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2019,10,9]],"date-time":"2019-10-09T00:00:00Z","timestamp":1570579200000}}],"container-title":["Cell Host & Microbe"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1931312819304160?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1931312819304160?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T12:18:40Z","timestamp":1761826720000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1931312819304160"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9]]},"references-count":28,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["S1931312819304160"],"URL":"https:\/\/doi.org\/10.1016\/j.chom.2019.08.007","relation":{},"ISSN":["1931-3128"],"issn-type":[{"value":"1931-3128","type":"print"}],"subject":[],"published":{"date-parts":[[2019,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Bioengineered Human Organ-on-Chip Reveals Intestinal Microenvironment and Mechanical Forces Impacting Shigella Infection","name":"articletitle","label":"Article Title"},{"value":"Cell Host & Microbe","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chom.2019.08.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Inc.","name":"copyright","label":"Copyright"}]}}