{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:13:51Z","timestamp":1792034031773,"version":"4.4.0"},"reference-count":98,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003006","name":"ETH Zurich","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003006","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Host & Microbe"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.chom.2026.08.005","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T14:41:34Z","timestamp":1788360094000},"page":"2028-2043.e8","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mobile virus-derived siRNAs drive plant antiviral silencing through reiterated 3\u20134 cell immunization"],"prefix":"10.1016","volume":"34","author":[{"given":"Daniele","family":"Albertini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuel A.","family":"Devers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory","family":"Schott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajendran","family":"Rajeswaran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u00eblle","family":"Jacquemettaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6982-9544","authenticated-orcid":false,"given":"Olivier","family":"Voinnet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.chom.2026.08.005_bib98","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1111\/j.0105-2896.2004.00128.x","article-title":"RNA silencing: no mercy for viruses?","volume":"198","author":"Lecellier","year":"2004","journal-title":"immunol. Rev."},{"key":"10.1016\/j.chom.2026.08.005_bib1","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1146\/annurev-virology-091919-064218","article-title":"The Role of Viruses in Identifying and Analyzing RNA Silencing","volume":"9","author":"Baulcombe","year":"2022","journal-title":"Annu. Rev. Virol."},{"key":"10.1016\/j.chom.2026.08.005_bib2","doi-asserted-by":"crossref","first-page":"12902","DOI":"10.1073\/pnas.0702500104","article-title":"Evidence that RNA silencing functions as an antiviral defense mechanism in fungi","volume":"104","author":"Segers","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chom.2026.08.005_bib3","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1146\/annurev-ento-033020-090410","article-title":"The Interplay Between Viruses and RNAi Pathways in Insects","volume":"66","author":"Bonning","year":"2021","journal-title":"Annu. Rev. Entomol."},{"key":"10.1016\/j.chom.2026.08.005_bib4","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1000586","article-title":"Natural and experimental infection of Caenorhabditis nematodes by novel viruses related to nodaviruses","volume":"9","author":"F\u00e9lix","year":"2011","journal-title":"PLoS Biol."},{"key":"10.1016\/j.chom.2026.08.005_bib5","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1126\/science.1241930","article-title":"Antiviral RNA interference in mammalian cells","volume":"342","author":"Maillard","year":"2013","journal-title":"Science"},{"key":"10.1016\/j.chom.2026.08.005_bib6","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.chom.2010.06.009","article-title":"Viral Suppressors of RNA-Based Viral Immunity: Host Targets","volume":"8","author":"Wu","year":"2010","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.chom.2026.08.005_bib7","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1038\/nrmicro3120","article-title":"RNA silencing suppression by plant pathogens: defence, counter-defence and counter-counter-defence","volume":"11","author":"Pumplin","year":"2013","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.chom.2026.08.005_bib8","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.virol.2015.02.028","article-title":"viral silencing suppressors: Tools forged to fine-tune host-pathogen coexistence","volume":"479\u2013480","author":"Csorba","year":"2015","journal-title":"Virology"},{"key":"10.1016\/j.chom.2026.08.005_bib9","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/S0092-8674(03)00984-X","article-title":"Size selective recognition of siRNA by an RNA silencing suppressor","volume":"115","author":"Vargason","year":"2003","journal-title":"Cell"},{"key":"10.1016\/j.chom.2026.08.005_bib10","doi-asserted-by":"crossref","first-page":"3347","DOI":"10.1038\/sj.emboj.7601217","article-title":"An antagonistic function for Arabidopsis DCL2 in development and a new function for DCL4 in generating viral siRNAs","volume":"25","author":"Bouch\u00e9","year":"2006","journal-title":"EMBO J."},{"key":"10.1016\/j.chom.2026.08.005_bib11","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1126\/science.1128214","article-title":"Hierarchical action and inhibition of plant Dicer-like proteins in antiviral defense","volume":"313","author":"Deleris","year":"2006","journal-title":"Science"},{"key":"10.1016\/j.chom.2026.08.005_bib12","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.cell.2007.07.039","article-title":"Antiviral Immunity Directed by Small RNAs","volume":"130","author":"Ding","year":"2007","journal-title":"Cell"},{"key":"10.1016\/j.chom.2026.08.005_bib13","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.1016\/j.cub.2007.07.061","article-title":"The Polerovirus F box protein P0 targets ARGONAUTE1 to suppress RNA silencing","volume":"17","author":"Bortolamiol","year":"2007","journal-title":"Curr. Biol."},{"key":"10.1016\/j.chom.2026.08.005_bib14","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1111\/j.1365-313X.2010.04163.x","article-title":"Polerovirus protein P0 prevents the assembly of small RNA-containing RISC complexes and leads to degradation of ARGONAUTE1","volume":"62","author":"Csorba","year":"2010","journal-title":"Plant J."},{"key":"10.1016\/j.chom.2026.08.005_bib15","doi-asserted-by":"crossref","first-page":"1609","DOI":"10.1016\/j.cub.2007.08.039","article-title":"The Polerovirus silencing suppressor P0 targets ARGONAUTE proteins for degradation","volume":"17","author":"Baumberger","year":"2007","journal-title":"Curr. Biol."},{"key":"10.1016\/j.chom.2026.08.005_bib16","doi-asserted-by":"crossref","first-page":"4738","DOI":"10.1093\/emboj\/16.15.4738","article-title":"Systemic acquired silencing: transgene-specific post-transcriptional silencing is transmitted by grafting from silenced stocks to non-silenced scions","volume":"16","author":"Palauqui","year":"1997","journal-title":"EMBO J."},{"key":"10.1016\/j.chom.2026.08.005_bib17","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/S0092-8674(00)81749-3","article-title":"Systemic spread of sequence-specific transgene RNA degradation in plants is initiated by localized introduction of ectopic promoterless DNA","volume":"95","author":"Voinnet","year":"1998","journal-title":"Cell"},{"key":"10.1016\/j.chom.2026.08.005_bib18","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1126\/science.1187959","article-title":"Small silencing RNAs in plants are mobile and direct epigenetic modification in recipient cells","volume":"328","author":"Molnar","year":"2010","journal-title":"Science"},{"key":"10.1016\/j.chom.2026.08.005_bib19","doi-asserted-by":"crossref","DOI":"10.1111\/tpj.70194","article-title":"A pectin acetyl-transferase facilitates secondary plasmodesmata formation and RNA silencing movement between plant cells","volume":"122","author":"Jay","year":"2025","journal-title":"Plant J."},{"key":"10.1016\/j.chom.2026.08.005_bib20","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/S1360-1385(96)10045-5","article-title":"Phloem loading and plasmodesmata","volume":"1","author":"Turgeon","year":"1996","journal-title":"Trends Plant Sci."},{"key":"10.1016\/j.chom.2026.08.005_bib21","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1038\/s41477-020-0687-2","article-title":"Movement and differential consumption of short interfering RNA duplexes underlie mobile RNA interference","volume":"6","author":"Devers","year":"2020","journal-title":"Nat. Plants"},{"key":"10.1016\/j.chom.2026.08.005_bib22","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1016\/j.devcel.2022.03.015","article-title":"Microtubules promote the non-cell autonomous action of microRNAs by inhibiting their cytoplasmic loading onto ARGONAUTE1 in Arabidopsis","volume":"57","author":"Fan","year":"2022","journal-title":"Dev. Cell"},{"key":"10.1016\/j.chom.2026.08.005_bib23","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/s41580-022-00455-0","article-title":"Revisiting small RNA movement in plants","volume":"23","author":"Voinnet","year":"2022","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.chom.2026.08.005_bib24","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.tplants.2008.04.007","article-title":"Plant ARGONAUTES","volume":"13","author":"Vaucheret","year":"2008","journal-title":"Trends Plant Sci."},{"key":"10.1016\/j.chom.2026.08.005_bib25","doi-asserted-by":"crossref","first-page":"1558","DOI":"10.1126\/science.276.5318.1558","article-title":"A similarity between viral defense and gene silencing in plants","volume":"276","author":"Ratcliff","year":"1997","journal-title":"Science"},{"key":"10.1016\/j.chom.2026.08.005_bib26","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1105\/tpc.10.6.937","article-title":"Initiation and maintenance of virus-induced gene silencing","volume":"10","author":"Ruiz","year":"1998","journal-title":"Plant Cell"},{"key":"10.1016\/j.chom.2026.08.005_bib27","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1105\/tpc.11.7.1207","article-title":"Gene silencing without DNA. rna-mediated cross-protection between viruses","volume":"11","author":"Ratcliff","year":"1999","journal-title":"Plant Cell"},{"key":"10.1016\/j.chom.2026.08.005_bib28","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0092-8674(00)00095-7","article-title":"A viral movement protein prevents spread of the gene silencing signal in Nicotiana benthamiana","volume":"103","author":"Voinnet","year":"2000","journal-title":"Cell"},{"key":"10.1016\/j.chom.2026.08.005_bib29","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/S0168-9525(01)02367-8","article-title":"RNA silencing as a plant immune system against viruses","volume":"17","author":"Voinnet","year":"2001","journal-title":"Trends Genet."},{"key":"10.1016\/j.chom.2026.08.005_bib30","doi-asserted-by":"crossref","first-page":"3553","DOI":"10.1038\/emboj.2011.274","article-title":"Intercellular and systemic movement of RNA silencing signals","volume":"30","author":"Melnyk","year":"2011","journal-title":"EMBO J."},{"key":"10.1016\/j.chom.2026.08.005_bib31","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1093\/jxb\/eraf312","article-title":"Three decades of mobile RNA silencing within plants: what have we learnt?","volume":"77","author":"Voinnet","year":"2026","journal-title":"J. Exp. Bot."},{"key":"10.1016\/j.chom.2026.08.005_bib32","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1038\/s41580-022-00496-5","article-title":"Roles of RNA silencing in viral and non-viral plant immunity and in the crosstalk between disease resistance systems","volume":"23","author":"Lopez-Gomollon","year":"2022","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.chom.2026.08.005_bib33","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.1104\/pp.105.063537","article-title":"An RNA-dependent RNA polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal","volume":"138","author":"Schwach","year":"2005","journal-title":"Plant Physiol."},{"key":"10.1016\/j.chom.2026.08.005_bib34","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2302069120","article-title":"Salicylic acid and RNA interference mediate antiviral immunity of plant stem cells","volume":"120","author":"Incarbone","year":"2023","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chom.2026.08.005_bib35","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1111\/j.1365-313X.2006.02796.x","article-title":"Phloem flow strongly influences the systemic spread of silencing in GFP Nicotiana benthamiana plants","volume":"47","author":"Tournier","year":"2006","journal-title":"Plant J."},{"key":"10.1016\/j.chom.2026.08.005_bib36","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1093\/jxb\/eraf184","article-title":"Regulation of cell-to-cell trafficking by viral movement proteins","volume":"77","author":"Alazem","year":"2026","journal-title":"J. Exp. Bot."},{"key":"10.1016\/j.chom.2026.08.005_bib37","doi-asserted-by":"crossref","first-page":"4523","DOI":"10.1093\/emboj\/cdg431","article-title":"Transitivity-dependent and -independent cell-to-cell movement of RNA silencing","volume":"22","author":"Himber","year":"2003","journal-title":"EMBO J."},{"key":"10.1016\/j.chom.2026.08.005_bib38","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1261\/rna.541307","article-title":"Transitivity in Arabidopsis can be primed, requires the redundant action of the antiviral Dicer-like 4 and Dicer-like 2, and is compromised by viral-encoded suppressor proteins","volume":"13","author":"Moissiard","year":"2007","journal-title":"Rna"},{"key":"10.1016\/j.chom.2026.08.005_bib39","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2020.579376","article-title":"The Whys and Wherefores of Transitivity in Plants","volume":"11","author":"de Felippes","year":"2020","journal-title":"Front. Plant Sci."},{"key":"10.1016\/j.chom.2026.08.005_bib40","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/S0092-8674(00)80864-8","article-title":"An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus","volume":"101","author":"Dalmay","year":"2000","journal-title":"Cell"},{"key":"10.1016\/j.chom.2026.08.005_bib41","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/S0092-8674(00)80863-6","article-title":"Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance","volume":"101","author":"Mourrain","year":"2000","journal-title":"Cell"},{"key":"10.1016\/j.chom.2026.08.005_bib42","doi-asserted-by":"crossref","first-page":"6082","DOI":"10.1128\/JVI.77.10.6082-6086.2003","article-title":"In situ characterization of Cymbidium Ringspot Tombusvirus infection-induced posttranscriptional gene silencing in Nicotiana benthamiana","volume":"77","author":"Havelda","year":"2003","journal-title":"J. Virol."},{"key":"10.1016\/j.chom.2026.08.005_bib43","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1101\/gad.1908710","article-title":"Argonaute quenching and global changes in Dicer homeostasis caused by a pathogen-encoded GW repeat protein","volume":"24","author":"Azevedo","year":"2010","journal-title":"Genes Dev."},{"key":"10.1016\/j.chom.2026.08.005_bib44","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1004755","article-title":"Roles and programming of Arabidopsis ARGONAUTE proteins during Turnip mosaic virus infection","volume":"11","author":"Garcia-Ruiz","year":"2015","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.chom.2026.08.005_bib45","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1105\/tpc.109.073056","article-title":"Arabidopsis RNA-Dependent RNA Polymerases and Dicer-Like Proteins in Antiviral Defense and Small Interfering RNA Biogenesis during Turnip Mosaic Virus Infection","volume":"22","author":"Garcia-Ruiz","year":"2010","journal-title":"Plant Cell"},{"key":"10.1016\/j.chom.2026.08.005_bib46","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1038\/nature08977","article-title":"Cell signalling by microRNA165\/6 directs gene dose-dependent root cell fate","volume":"465","author":"Carlsbecker","year":"2010","journal-title":"Nature"},{"key":"10.1016\/j.chom.2026.08.005_bib47","doi-asserted-by":"crossref","DOI":"10.15252\/embj.2018100754","article-title":"Genome-scale, single-cell-type resolution of microRNA activities within a whole plant organ","volume":"38","author":"Brosnan","year":"2019","journal-title":"EMBO J."},{"key":"10.1016\/j.chom.2026.08.005_bib48","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1038\/s44318-024-00071-y","article-title":"An AGO10:miR165\/6 module regulates meristem activity and xylem development in the Arabidopsis root","volume":"43","author":"Mirlohi","year":"2024","journal-title":"EMBO J."},{"key":"10.1016\/j.chom.2026.08.005_bib49","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.1038\/s44318-024-00072-x","article-title":"ARGONAUTE10 controls cell fate specification and formative cell divisions in the Arabidopsis root","volume":"43","author":"El Arbi","year":"2024","journal-title":"EMBO J."},{"key":"10.1016\/j.chom.2026.08.005_bib50","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1146\/annurev-arplant-042811-105501","article-title":"Control of Arabidopsis root development","volume":"63","author":"Petricka","year":"2012","journal-title":"Annu. Rev. Plant Biol."},{"key":"10.1016\/j.chom.2026.08.005_bib51","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.24125","article-title":"Phloem unloading in Arabidopsis roots is convective and regulated by the phloem-pole pericycle","volume":"6","author":"Ross-Elliott","year":"2017","journal-title":"eLife"},{"key":"10.1016\/j.chom.2026.08.005_bib52","series-title":"eLS","first-page":"1","article-title":"Companion Cell and Sieve Elements","author":"Kim","year":"2023"},{"key":"10.1016\/j.chom.2026.08.005_bib53","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1146\/annurev-virology-010320-072410","article-title":"Viral Hacks of the Plant Vasculature: The Role of Phloem Alterations in Systemic Virus Infection","volume":"7","author":"Kappagantu","year":"2020","journal-title":"Annu. Rev. Virol."},{"key":"10.1016\/j.chom.2026.08.005_bib54","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/0042-6822(80)90039-2","article-title":"Ultrastructure of potato leaf phloem infected with potato leafroll virus","volume":"105","author":"Shepardson","year":"1980","journal-title":"Virology"},{"key":"10.1016\/j.chom.2026.08.005_bib55","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1094\/PDIS-92-1-0051","article-title":"Biological and Molecular Characterization of an American Sugar Beet-Infecting Beet western yellows virus Isolate","volume":"92","author":"Beuve","year":"2008","journal-title":"Plant Dis."},{"key":"10.1016\/j.chom.2026.08.005_bib56","doi-asserted-by":"crossref","first-page":"154","DOI":"10.3389\/fpls.2013.00154","article-title":"Viral and cellular factors involved in Phloem transport of plant viruses","volume":"4","author":"Hipper","year":"2013","journal-title":"Front. Plant Sci."},{"key":"10.1016\/j.chom.2026.08.005_bib57","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1004868","article-title":"Discovery of a Small Non-AUG-Initiated ORF in Poleroviruses and Luteoviruses That Is Required for Long-Distance Movement","volume":"11","author":"Smirnova","year":"2015","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.chom.2026.08.005_bib58","doi-asserted-by":"crossref","first-page":"166","DOI":"10.3390\/v9070166","article-title":"Systemic Propagation of a Fluorescent Infectious Clone of a Polerovirus Following Inoculation by Agrobacteria and Aphids","volume":"9","author":"Boissinot","year":"2017","journal-title":"Viruses"},{"key":"10.1016\/j.chom.2026.08.005_bib59","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.ibmb.2014.12.005","article-title":"The structural sheath protein of aphids is required for phloem feeding","volume":"57","author":"Will","year":"2015","journal-title":"Insect Biochem. Mol. Biol."},{"key":"10.1016\/j.chom.2026.08.005_bib60","doi-asserted-by":"crossref","first-page":"11274","DOI":"10.1093\/nar\/gkab802","article-title":"Atypical molecular features of RNA silencing against the phloem-restricted polerovirus TuYV","volume":"49","author":"Clavel","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.chom.2026.08.005_bib61","doi-asserted-by":"crossref","first-page":"4765","DOI":"10.1242\/dev.02055","article-title":"The WEREWOLF MYB protein directly regulates CAPRICE transcription during cell fate specification in the Arabidopsis root epidermis","volume":"132","author":"Ryu","year":"2005","journal-title":"Development"},{"key":"10.1016\/j.chom.2026.08.005_bib62","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/s13062-024-00535-5","article-title":"Shaping root architecture: towards understanding the mechanisms involved in lateral root development","volume":"19","author":"Yalamanchili","year":"2024","journal-title":"Biol. Direct"},{"key":"10.1016\/j.chom.2026.08.005_bib64","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1016\/j.cell.2006.03.037","article-title":"Perception of the bacterial PAMP EF-Tu by the receptor EFR restricts Agrobacterium-mediated transformation","volume":"125","author":"Zipfel","year":"2006","journal-title":"Cell"},{"key":"10.1016\/j.chom.2026.08.005_bib65","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1111\/tpj.16032","article-title":"A contemporary reassessment of the enhanced transient expression system based on the tombusviral silencing suppressor protein P19","volume":"113","author":"Jay","year":"2023","journal-title":"Plant J."},{"key":"10.1016\/j.chom.2026.08.005_bib66","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1111\/nph.18180","article-title":"Suppression of both intra- and intercellular RNA silencing by the tombusviral P19 protein requires its small RNA binding property","volume":"235","author":"Brioudes","year":"2022","journal-title":"New Phytol."},{"key":"10.1016\/j.chom.2026.08.005_bib63","article-title":"Synergistic infection of BrYV and PEMV 2 increases the accumulations of both BrYV and BrYV-derived siRNAs in Nicotiana benthamiana","volume":"7","author":"Zhou","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.chom.2026.08.005_bib67","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1111\/j.1365-313X.2007.03338.x","article-title":"Evidence for a sorting endosome in Arabidopsis root cells","volume":"53","author":"Jaillais","year":"2008","journal-title":"Plant J."},{"key":"10.1016\/j.chom.2026.08.005_bib68","doi-asserted-by":"crossref","first-page":"18843","DOI":"10.1073\/pnas.0906131106","article-title":"Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis","volume":"106","author":"Mustroph","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chom.2026.08.005_bib69","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1093\/plphys\/kiab474","article-title":"Asymmetric expression of Argonautes in reproductive tissues","volume":"188","author":"Jullien","year":"2022","journal-title":"Plant Physiol."},{"key":"10.1016\/j.chom.2026.08.005_bib70","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1101\/gad.1201404","article-title":"The action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant development","volume":"18","author":"Vaucheret","year":"2004","journal-title":"Genes Dev."},{"key":"10.1016\/j.chom.2026.08.005_bib71","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.cell.2005.04.004","article-title":"microRNA-directed phasing during trans-acting siRNA biogenesis in plants","volume":"121","author":"Allen","year":"2005","journal-title":"Cell"},{"key":"10.1016\/j.chom.2026.08.005_bib72","doi-asserted-by":"crossref","first-page":"15942","DOI":"10.1073\/pnas.1209487109","article-title":"Degradation of the antiviral component ARGONAUTE1 by the autophagy pathway","volume":"109","author":"Derrien","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chom.2026.08.005_bib73","doi-asserted-by":"crossref","first-page":"9339","DOI":"10.1093\/nar\/gkr590","article-title":"The miRNA pathway limits AGO1 availability during siRNA-mediated PTGS defense against exogenous RNA","volume":"39","author":"Mart\u00ednez de Alba","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.chom.2026.08.005_bib74","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.cell.2007.04.037","article-title":"Molecular Basis for Target RNA Recognition and Cleavage by Human RISC","volume":"130","author":"Ameres","year":"2007","journal-title":"Cell"},{"key":"10.1016\/j.chom.2026.08.005_bib75","doi-asserted-by":"crossref","first-page":"2980","DOI":"10.1128\/JVI.02339-06","article-title":"A structured viroid RNA serves as a substrate for dicer-like cleavage to produce biologically active small RNAs but is resistant to RNA-induced silencing complex-mediated degradation","volume":"81","author":"Itaya","year":"2007","journal-title":"J. Virol."},{"key":"10.1016\/j.chom.2026.08.005_bib76","first-page":"115","article-title":"Meristem-tip culture for propagation and virus elimination","volume":"111","author":"Grout","year":"1999","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.chom.2026.08.005_bib77","doi-asserted-by":"crossref","DOI":"10.15252\/embj.2021109902","article-title":"Mouse circulating extracellular vesicles contain virus-derived siRNAs active in antiviral immunity","volume":"41","author":"Zhang","year":"2022","journal-title":"EMBO J."},{"key":"10.1016\/j.chom.2026.08.005_bib78","article-title":"Live-attenuated virus vaccine defective in RNAi suppression induces rapid protection in neonatal and adult mice lacking mature B and T cells","volume":"121","author":"Chen","year":"2024","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chom.2026.08.005_bib79","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1094\/MPMI.2001.14.8.939","article-title":"Dark green islands in plant virus infection are the result of posttranscriptional gene silencing","volume":"14","author":"Moore","year":"2001","journal-title":"Mol. Plant Microbe Interact."},{"key":"10.1016\/j.chom.2026.08.005_bib80","doi-asserted-by":"crossref","first-page":"3250","DOI":"10.1128\/JVI.02139-07","article-title":"Antiviral RNA silencing is restricted to the marginal region of the dark green tissue in the mosaic leaves of tomato mosaic virus-infected tobacco plants","volume":"82","author":"Hirai","year":"2008","journal-title":"J. Virol."},{"key":"10.1016\/j.chom.2026.08.005_bib81","doi-asserted-by":"crossref","first-page":"5419","DOI":"10.1128\/JVI.02312-08","article-title":"The C terminus of the polerovirus p5 readthrough domain limits virus infection to the phloem","volume":"83","author":"Peter","year":"2009","journal-title":"J. Virol."},{"key":"10.1016\/j.chom.2026.08.005_bib82","doi-asserted-by":"crossref","first-page":"6815","DOI":"10.1128\/JVI.76.13.6815-6824.2002","article-title":"P0 of beet Western yellows virus is a suppressor of posttranscriptional gene silencing","volume":"76","author":"Pfeffer","year":"2002","journal-title":"J. Virol."},{"key":"10.1016\/j.chom.2026.08.005_bib88","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1002\/j.1460-2075.1995.tb07043.x","article-title":"Aphid transmission of beet western yellows luteovirus requires the minor capsid read-through protein P74","volume":"14","author":"Brault","year":"1995","journal-title":"EMBO J."},{"key":"10.1016\/j.chom.2026.08.005_bib83","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1105\/tpc.105.031625","article-title":"Auxin and light control of adventitious rooting in Arabidopsis require ARGONAUTE1","volume":"17","author":"Sorin","year":"2005","journal-title":"Plant Cell"},{"key":"10.1016\/j.chom.2026.08.005_bib84","doi-asserted-by":"crossref","first-page":"12984","DOI":"10.1073\/pnas.0506426102","article-title":"DICER-LIKE 4 functions in trans-acting small interfering RNA biogenesis and vegetative phase change in Arabidopsis thaliana","volume":"102","author":"Xie","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chom.2026.08.005_bib85","doi-asserted-by":"crossref","first-page":"5003","DOI":"10.1093\/nar\/gkr119","article-title":"Massive production of small RNAs from a non-coding region of Cauliflower mosaic virus in plant defense and viral counter-defense","volume":"39","author":"Blevins","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.chom.2026.08.005_bib86","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1111\/tpj.14868","article-title":"Functional characterization of Arabidopsis ARGONAUTE 3 in reproductive tissues","volume":"103","author":"Jullien","year":"2020","journal-title":"Plant J."},{"key":"10.1016\/j.chom.2026.08.005_bib87","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.devcel.2014.05.020","article-title":"Regulation of plant stem cell quiescence by a brassinosteroid signaling module","volume":"30","author":"Vilarrasa-Blasi","year":"2014","journal-title":"Dev. Cell"},{"key":"10.1016\/j.chom.2026.08.005_bib89","doi-asserted-by":"crossref","first-page":"1994","DOI":"10.1073\/pnas.0510784103","article-title":"F-box-like domain in the polerovirus protein P0 is required for silencing suppressor function","volume":"103","author":"Pazhouhandeh","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chom.2026.08.005_bib90","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.1101\/gad.307804","article-title":"In vivo investigation of the transcription, processing, endonucleolytic activity, and functional relevance of the spatial distribution of a plant miRNA","volume":"18","author":"Parizotto","year":"2004","journal-title":"Genes Dev."},{"key":"10.1016\/j.chom.2026.08.005_bib91","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/j.molcel.2018.01.007","article-title":"Nucleo-cytosolic Shuttling of ARGONAUTE1 Prompts a Revised Model of the Plant MicroRNA Pathway","volume":"69","author":"Bologna","year":"2018","journal-title":"Mol. Cell"},{"key":"10.1016\/j.chom.2026.08.005_bib92","doi-asserted-by":"crossref","DOI":"10.15252\/embj.2020107455","article-title":"HASTY, the Arabidopsis EXPORTIN5 ortholog, regulates cell-to-cell and vascular microRNA movement","volume":"40","author":"Brioudes","year":"2021","journal-title":"EMBO J."},{"key":"10.1016\/j.chom.2026.08.005_bib93","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1104\/pp.107.106989","article-title":"Recombinational cloning with plant gateway vectors","volume":"145","author":"Karimi","year":"2007","journal-title":"Plant Physiol."},{"key":"10.1016\/j.chom.2026.08.005_bib94","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1038\/nprot.2006.97","article-title":"Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method","volume":"1","author":"Zhang","year":"2006","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.chom.2026.08.005_bib95","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkaa472","article-title":"A universal method for the rapid isolation of all known classes of functional silencing small RNAs","volume":"48","author":"Grentzinger","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.chom.2026.08.005_bib96","first-page":"143","article-title":"Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method","volume":"2019","author":"Green","year":"2019","journal-title":"Cold Spring Harb. Protoc."},{"key":"10.1016\/j.chom.2026.08.005_bib97","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/1746-4811-3-12","article-title":"Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs","volume":"3","author":"Varkonyi-Gasic","year":"2007","journal-title":"Plant Methods"}],"container-title":["Cell Host & Microbe"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1931312826003252?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1931312826003252?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T15:11:59Z","timestamp":1791990719000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1931312826003252"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":98,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["S1931312826003252"],"URL":"https:\/\/doi.org\/10.1016\/j.chom.2026.08.005","relation":{},"ISSN":["1931-3128"],"issn-type":[{"value":"1931-3128","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Mobile virus-derived siRNAs drive plant antiviral silencing through reiterated 3\u20134 cell immunization","name":"articletitle","label":"Article Title"},{"value":"Cell Host & Microbe","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chom.2026.08.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}