{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T01:15:46Z","timestamp":1774055746244,"version":"3.50.1"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,11,1]],"date-time":"2016-11-01T00:00:00Z","timestamp":1477958400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"NSFC of China","award":["21545007"],"award-info":[{"award-number":["21545007"]}]},{"name":"Shaanxi Provincial Science and Technology Coordinating Innovation Projects","award":["2013SZS18-K01"],"award-info":[{"award-number":["2013SZS18-K01"]}]},{"name":"Foundation of Key Laboratory in Shaanxi Province","award":["14JS098"],"award-info":[{"award-number":["14JS098"]}]},{"name":"Foundation of Key Laboratory in Shaanxi Province","award":["15JS115"],"award-info":[{"award-number":["15JS115"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography A"],"published-print":{"date-parts":[[2016,11]]},"DOI":"10.1016\/j.chroma.2016.10.025","type":"journal-article","created":{"date-parts":[[2016,10,16]],"date-time":"2016-10-16T17:10:59Z","timestamp":1476637859000},"page":"138-144","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":35,"special_numbering":"C","title":["Preparation and characterization of monodisperse large-porous silica microspheres as the matrix for protein separation"],"prefix":"10.1016","volume":"1471","author":[{"given":"Hongjun","family":"Xia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangping","family":"Wan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junlong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiawei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quan","family":"Bai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.chroma.2016.10.025_bib0005","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.jpba.2013.04.027","article-title":"Critical practical aspects in the application of liquid chromatography\u2013mass spectrometric studies for the characterization of impurities and degradation products","volume":"87","author":"Narayanam","year":"2014","journal-title":"J. Pharm. Biomed."},{"key":"10.1016\/j.chroma.2016.10.025_bib0010","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.chroma.2012.08.087","article-title":"Challenges in the development of bioanalytical liquid chromatography\u2013mass spectrometry method with emphasis on fast analysis","volume":"1292","author":"Nov\u00e1kov\u00e1","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2016.10.025_bib0015","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.chroma.2012.08.069","article-title":"What is the future of (ultra) high performance liquid chromatography coupled to low and high resolution mass spectrometry for toxicological drug screening","volume":"1292","author":"Maurer","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2016.10.025_bib0020","doi-asserted-by":"crossref","first-page":"10083","DOI":"10.1021\/ac402002m","article-title":"Online and splitless nanoLC\u00d7capillaryLC with quadrupole\/time-of-flight mass spectrometric detection for comprehensive screening analysis of complex samples","volume":"85","author":"Haun","year":"2013","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2016.10.025_bib0025","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1007\/s13361-012-0508-6","article-title":"Effects of column and gradient lengths on peak capacity and peptide identification in nanoflow LC\u2013MS\/MS of complex proteomic samples","volume":"24","author":"Hsieh","year":"2013","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"10.1016\/j.chroma.2016.10.025_bib0030","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.chroma.2014.05.010","article-title":"Core\u2013shell particles: preparation, fundamentals and applications in high performance liquid chromatography","volume":"1357","author":"Hayes","year":"2014","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2016.10.025_bib0035","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0021-9673(96)00205-1","article-title":"Comparison of an ordered mesoporous aluminosilicate silica, alumina, titania and zirconia in normal-phase high-performance liquid chromatography","volume":"740","author":"Gr\u00fcn","year":"1996","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2016.10.025_bib0040","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/0021-9797(68)90272-5","article-title":"Controlled growth of monodisperse silica spheres in the micron size range","volume":"26","author":"St\u00f6ber","year":"1968","journal-title":"J. Colloid Interface Sci."},{"key":"10.1016\/j.chroma.2016.10.025_bib0045","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1021\/la048569r","article-title":"Smart control of monodisperse St\u00f6ber silica particles: effect of reactant addition rate on growth process","volume":"21","author":"Nozawa","year":"2005","journal-title":"Langmuir"},{"key":"10.1016\/j.chroma.2016.10.025_bib0050","doi-asserted-by":"crossref","first-page":"7512","DOI":"10.1021\/la904316f","article-title":"Electrolyte-added one-pot synthesis for producing monodisperse, micrometer-sized silica particles up to 7\u03bcm","volume":"26","author":"Nakabayashi","year":"2010","journal-title":"Langmuir"},{"key":"10.1016\/j.chroma.2016.10.025_bib0055","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1039\/a705746f","article-title":"Synthesis of mesoporous silica spheres under quiescent aqueous acidic conditions","volume":"8","author":"Yang","year":"1998","journal-title":"J. Mater. Chem."},{"key":"10.1016\/j.chroma.2016.10.025_bib0060","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1002\/(SICI)1521-4095(199912)11:17<1452::AID-ADMA1452>3.0.CO;2-R","article-title":"The use of mesoporous silica in liquid chromatography","volume":"11","author":"Gallis","year":"1999","journal-title":"Adv. Mater."},{"key":"10.1016\/j.chroma.2016.10.025_bib0065","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1126\/science.269.5228.1242","article-title":"Templating of mesoporous molecular sieves by nonionic polyethylene oxide surfactants","volume":"269","author":"Bagshaw","year":"1995","journal-title":"Science"},{"key":"10.1016\/j.chroma.2016.10.025_bib0070","doi-asserted-by":"crossref","first-page":"4112","DOI":"10.1039\/b510224c","article-title":"Synthesis of super-microporous organosilica microspheres through in situ self-assembly of nanoparticles","volume":"15","author":"Guo","year":"2005","journal-title":"J. Mater. Chem."},{"key":"10.1016\/j.chroma.2016.10.025_bib0075","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1016\/j.jcis.2006.02.043","article-title":"Synthesis of micrometer-sized hard silica spheres with uniform mesopore size and textural pores","volume":"299","author":"Yang","year":"2006","journal-title":"J. Colloid Interface Sci."},{"key":"10.1016\/j.chroma.2016.10.025_bib0080","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1021\/ie901213v","article-title":"Synthesis of uniform porous silica microspheres with hydrophilic polymer as stabilizing agent","volume":"49","author":"Ahmed","year":"2009","journal-title":"Ind. Eng. Chem. Res."},{"key":"10.1016\/j.chroma.2016.10.025_bib0085","doi-asserted-by":"crossref","first-page":"19500","DOI":"10.1039\/C4TA05002A","article-title":"Formation of uniform large SBA-15 microspheres via spray drying","volume":"2","author":"Waldron","year":"2014","journal-title":"J. Mater. Chem. A"},{"key":"10.1016\/j.chroma.2016.10.025_bib0090","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1080\/01932691.2015.1022655","article-title":"Fabrication of hollow or macroporous silica particles by spray drying of colloidal dispersion","volume":"37","author":"Cho","year":"2016","journal-title":"J. Dispers. Sci. Technol."},{"key":"10.1016\/j.chroma.2016.10.025_bib0095","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.jcis.2013.12.027","article-title":"Formation of monodisperse mesoporous silica microparticles via spray-drying","volume":"418","author":"Waldron","year":"2014","journal-title":"J. Colloid Interface Sci."},{"key":"10.1016\/j.chroma.2016.10.025_bib0100","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1021\/ac00147a013","article-title":"Synthesis and characterization of 2\u03bcm wide-pore silica microspheres as column packings for the reversed-phase liquid chromatography of peptides and proteins","volume":"59","author":"Danielson","year":"1987","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2016.10.025_bib0105","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.4028\/www.scientific.net\/KEM.280-283.1153","article-title":"Preparation and formation mechanisms of monodispersed mesoporous SiO2 microspheres by the PICA method","volume":"280","author":"Zhao","year":"2005","journal-title":"Key Eng. Mater."},{"key":"10.1016\/j.chroma.2016.10.025_bib0110","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.partic.2014.08.005","article-title":"Preparation of high-purity monodisperse silica microspheres by the sol\u2013gel method coupled with polymerization-induced colloid aggregation","volume":"22","author":"Zhao","year":"2015","journal-title":"Particuology"},{"key":"10.1016\/j.chroma.2016.10.025_bib0115","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1002\/jssc.200500511","article-title":"Spherical ordered mesoporous silicas and silica monoliths as stationary phases for liquid chromatography","volume":"29","author":"Galarneau","year":"2006","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.chroma.2016.10.025_bib0120","doi-asserted-by":"crossref","first-page":"1768","DOI":"10.1002\/1521-4095(20021203)14:23<1768::AID-ADMA1768>3.0.CO;2-0","article-title":"Templating of porous polymeric beads to form porous silica and titania spheres","volume":"14","author":"Meyer","year":"2002","journal-title":"Adv. Mater."},{"key":"10.1016\/j.chroma.2016.10.025_bib0125","doi-asserted-by":"crossref","first-page":"2889","DOI":"10.1002\/pola.26066","article-title":"Preparation and characterization of monodisperse porous silica microspheres with controllable morphology and structure","volume":"50","author":"He","year":"2012","journal-title":"J. Polym. Sci. Polym. Chem"},{"key":"10.1016\/j.chroma.2016.10.025_bib0130","doi-asserted-by":"crossref","first-page":"3104","DOI":"10.1021\/ma00214a012","article-title":"Dispersion polymerization of styrene in polar solvents. 6. Influence of reaction parameters on particle size and molecular weight in poly (N-vinylpyrrolidone)-stabilized reactions","volume":"23","author":"Paine","year":"1990","journal-title":"Macromolecules"},{"key":"10.1016\/j.chroma.2016.10.025_bib0135","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1007\/s00216-003-1805-5","article-title":"Preparation of weak cation exchange packings based on monodisperse poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads and their chromatog-raphic properties","volume":"375","author":"Gong","year":"2003","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.chroma.2016.10.025_bib0140","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1002\/cjoc.20040220315","article-title":"Synthesis of monodisperse poly(glycidylmethacrylate-co-et-hylene dimethacrylate) beads and their application in separation of biopolymers","volume":"22","author":"Gong","year":"2004","journal-title":"Chin. J. Chem."},{"key":"10.1016\/j.chroma.2016.10.025_bib0145","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1021\/ac950914h","article-title":"Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels","volume":"68","author":"Shevchenko","year":"1996","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2016.10.025_bib0150","doi-asserted-by":"crossref","first-page":"1697","DOI":"10.1021\/ac0518304","article-title":"Improved protein recovery in reversed-phase liquid chromatography by the use of ultrahigh pressures","volume":"78","author":"Eschelbach","year":"2006","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2016.10.025_bib0155","first-page":"29","article-title":"Preparation of monodispersed microspheres with polymer core and SiO2 shell structure","volume":"39","author":"Ji","year":"2011","journal-title":"Chem. Eng."}],"container-title":["Journal of Chromatography A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967316313723?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967316313723?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,9,7]],"date-time":"2018-09-07T11:53:23Z","timestamp":1536321203000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021967316313723"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11]]},"references-count":31,"alternative-id":["S0021967316313723"],"URL":"https:\/\/doi.org\/10.1016\/j.chroma.2016.10.025","relation":{},"ISSN":["0021-9673"],"issn-type":[{"value":"0021-9673","type":"print"}],"subject":[],"published":{"date-parts":[[2016,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Preparation and characterization of monodisperse large-porous silica microspheres as the matrix for protein separation","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography A","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chroma.2016.10.025","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}