{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T11:10:54Z","timestamp":1791457854458,"version":"4.3.4"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography A"],"published-print":{"date-parts":[[2017,1]]},"DOI":"10.1016\/j.chroma.2016.12.033","type":"journal-article","created":{"date-parts":[[2016,12,14]],"date-time":"2016-12-14T18:31:44Z","timestamp":1481740304000},"page":"137-144","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":50,"special_numbering":"C","title":["Assay to measure sphingomyelinase and ceramidase activities efficiently and safely"],"prefix":"10.1016","volume":"1481","author":[{"given":"Christiane","family":"M\u00fchle","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Kornhuber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.chroma.2016.12.033_bib0005","doi-asserted-by":"crossref","first-page":"2754","DOI":"10.1016\/j.chroma.2010.11.066","article-title":"Lipid analysis by thin-layer chromatography\u2013a review of the current state","volume":"1218","author":"Fuchs","year":"2011","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2016.12.033_bib0010","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/S0076-6879(00)12900-3","article-title":"Sphingolipid extraction and analysis by thin-layer chromatography","volume":"312","author":"van Echten-Deckert","year":"2000","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.chroma.2016.12.033_bib0015","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1111\/jnc.13537","article-title":"A sphingolipid mechanism for behavioral extinction","volume":"137","author":"Huston","year":"2016","journal-title":"J. Neurochem."},{"key":"10.1016\/j.chroma.2016.12.033_bib0020","doi-asserted-by":"crossref","first-page":"405","DOI":"10.2174\/1566524043360573","article-title":"Sphingolipids in inflammation: roles and implications","volume":"4","author":"Pettus","year":"2004","journal-title":"Curr. Mol. Med."},{"issue":"Pt. (1)","key":"10.1016\/j.chroma.2016.12.033_bib0025","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1042\/bj3080269","article-title":"Localization of cholesterol in sphingomyelinase-treated fibroblasts","volume":"308","author":"Porn","year":"1995","journal-title":"Biochem. J."},{"key":"10.1016\/j.chroma.2016.12.033_bib0030","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1073\/pnas.55.2.366","article-title":"The metabolism of sphingomyelin. II. Evidence of an enzymatic deficiency in niemann-pick disease","volume":"55","author":"Brady","year":"1966","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.chroma.2016.12.033_bib0035","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1546-0096-5-15","article-title":"Farber disease: clinical presentation, pathogenesis and a new approach to treatment","volume":"5","author":"Ehlert","year":"2007","journal-title":"Pediatr. Rheumatol. Online J."},{"key":"10.1016\/j.chroma.2016.12.033_bib0040","doi-asserted-by":"crossref","first-page":"3419","DOI":"10.1096\/fj.08-108043","article-title":"The unexpected role of acid sphingomyelinase in cell death and the pathophysiology of common diseases","volume":"22","author":"Smith","year":"2008","journal-title":"FASEB J."},{"key":"10.1016\/j.chroma.2016.12.033_bib0045","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1515\/hsz-2015-0109","article-title":"Secretory sphingomyelinase in health and disease","volume":"396","author":"Kornhuber","year":"2015","journal-title":"Biol. Chem."},{"key":"10.1016\/j.chroma.2016.12.033_bib0050","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1111\/j.1530-0277.2009.01064.x","article-title":"Increased acid sphingomyelinase activity in peripheral blood cells of acutely intoxicated patients with alcohol dependence","volume":"34","author":"Reichel","year":"2010","journal-title":"Alcohol Clin. Exp. Res."},{"key":"10.1016\/j.chroma.2016.12.033_bib0055","doi-asserted-by":"crossref","first-page":"1852","DOI":"10.1111\/j.1530-0277.2011.01529.x","article-title":"Activity of secretory sphingomyelinase is increased in plasma of alcohol-dependent patients","volume":"35","author":"Reichel","year":"2011","journal-title":"Alcohol Clin. Exp. Res."},{"key":"10.1016\/j.chroma.2016.12.033_bib0060","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1159\/000362985","article-title":"Sex-dependent decrease of sphingomyelinase activity during alcohol withdrawal treatment","volume":"34","author":"M\u00fchle","year":"2014","journal-title":"Cell. Physiol. Biochem."},{"key":"10.1016\/j.chroma.2016.12.033_bib0065","doi-asserted-by":"crossref","first-page":"e62912","DOI":"10.1371\/journal.pone.0062912","article-title":"Characterization of acid sphingomyelinase activity in human cerebrospinal fluid","volume":"8","author":"M\u00fchle","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.chroma.2016.12.033_bib0070","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1111\/j.0022-202X.2004.22340.x","article-title":"Keratinocytes rapidly readjust ceramide-sphingomyelin homeostasis and contain a phosphatidylcholine-sphingomyelin transacylase","volume":"122","author":"Wanner","year":"2004","journal-title":"J. Invest. Dermatol."},{"key":"10.1016\/j.chroma.2016.12.033_bib0075","doi-asserted-by":"crossref","first-page":"e105830","DOI":"10.1371\/journal.pone.0105830","article-title":"Identification and biochemical characterization of an acid sphingomyelinase-like protein from the bacterial plant pathogen ralstonia solanacearum that hydrolyzes atp to amp but not sphingomyelin to ceramide","volume":"9","author":"Airola","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.chroma.2016.12.033_bib0080","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1159\/000447789","article-title":"Regulation of neuronal stem cell proliferation in the hippocampus by endothelial ceramide","volume":"39","author":"Gulbins","year":"2016","journal-title":"Cell. Physiol. Biochem."},{"key":"10.1016\/j.chroma.2016.12.033_bib0085","doi-asserted-by":"crossref","first-page":"e0161443","DOI":"10.1371\/journal.pone.0161443","article-title":"Chemokine receptors, cxcr1 and cxcr2, differentially regulate exosome release in hepatocytes","volume":"11","author":"Nojima","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.chroma.2016.12.033_bib0090","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/S0003-2697(02)00629-2","article-title":"A fluorescence-based, high-performance liquid chromatographic assay to determine acid sphingomyelinase activity and diagnose types a and b niemann-pick disease","volume":"314","author":"He","year":"2003","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.chroma.2016.12.033_bib0095","doi-asserted-by":"crossref","first-page":"525","DOI":"10.4062\/biomolther.2015.044","article-title":"Vitamin c stimulates epidermal ceramide production by regulating its metabolic enzymes","volume":"23","author":"Kim","year":"2015","journal-title":"Biomol. Ther. (Seoul)"},{"key":"10.1016\/j.chroma.2016.12.033_bib0100","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.5551\/jat.32383","article-title":"Acid sphingomyelinase mediates oxidized-ldl induced apoptosis in macrophage via endoplasmic reticulum stress","volume":"23","author":"Zhao","year":"2016","journal-title":"J. Atheroscler. Thromb."},{"key":"10.1016\/j.chroma.2016.12.033_bib0105","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1006\/abio.1999.4284","article-title":"A fluorescence-based high-performance liquid chromatographic assay to determine acid ceramidase activity","volume":"274","author":"He","year":"1999","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.chroma.2016.12.033_bib0110","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1006\/abio.1997.2391","article-title":"A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: applications in detecting the activity of phagocyte nadph oxidase and other oxidases","volume":"253","author":"Zhou","year":"1997","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.chroma.2016.12.033_bib0115","doi-asserted-by":"crossref","first-page":"12196","DOI":"10.1038\/ncomms12196","article-title":"Crystal structure of mammalian acid sphingomyelinase","volume":"7","author":"Gorelik","year":"2016","journal-title":"Nat. Commun."},{"key":"10.1016\/j.chroma.2016.12.033_bib0120","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1373\/clinchem.2014.236448","article-title":"Reliable assay of acid sphingomyelinase deficiency with the mutation q292k by tandem mass spectrometry","volume":"61","author":"Ghomashchi","year":"2015","journal-title":"Clin. Chem."},{"key":"10.1016\/j.chroma.2016.12.033_bib0125","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1002\/cbic.200600533","article-title":"Synthesis of a novel ceramide analogue and its use in a high-throughput fluorogenic assay for ceramidases","volume":"8","author":"Bedia","year":"2007","journal-title":"ChemBioChem"},{"key":"10.1016\/j.chroma.2016.12.033_bib0130","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1515\/hsz-2015-0266","article-title":"Increased secretory sphingomyelinase activity in the first trimester of pregnancy in women later developing preeclampsia: a nested case-control study","volume":"397","author":"Rodriguez-Sureda","year":"2016","journal-title":"Biol. Chem."},{"key":"10.1016\/j.chroma.2016.12.033_bib0135","doi-asserted-by":"crossref","first-page":"e23852","DOI":"10.1371\/journal.pone.0023852","article-title":"Identification of novel functional inhibitors of acid sphingomyelinase","volume":"6","author":"Kornhuber","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.chroma.2016.12.033_bib0140","doi-asserted-by":"crossref","first-page":"e35467","DOI":"10.1371\/journal.pone.0035467","article-title":"Functional implications of novel human acid sphingomyelinase splice variants","volume":"7","author":"Rhein","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.chroma.2016.12.033_bib0145","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/S0076-6879(00)22036-3","article-title":"Measurement of sphingomyelinase activity","volume":"322","author":"Gulbins","year":"2000","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.chroma.2016.12.033_bib0150","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1186\/s12944-015-0137-8","article-title":"Mevalonate inhibits acid sphingomyelinase activity, increases sphingomyelin levels and inhibits cell proliferation of HepG2 and CaCo-2 cells","volume":"14","author":"Chen","year":"2015","journal-title":"Lipids Health Dis."},{"key":"10.1016\/j.chroma.2016.12.033_bib0155","doi-asserted-by":"crossref","first-page":"2307","DOI":"10.3390\/ijms16022307","article-title":"Gentamicin arrests cancer cell growth: the intriguing involvement of nuclear sphingomyelin metabolism","volume":"16","author":"Codini","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.chroma.2016.12.033_bib0160","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/S0022-2275(20)30124-3","article-title":"High-precision fluorescence assay for sphingomyelinase activity of isolated enzymes and cell lysates","volume":"43","author":"Loidl","year":"2002","journal-title":"J. Lipid Res."},{"key":"10.1016\/j.chroma.2016.12.033_bib0165","doi-asserted-by":"crossref","first-page":"32978","DOI":"10.1074\/jbc.M301936200","article-title":"Purification and characterization of recombinant, human acid ceramidase: catalytic reactions and interactions with acid sphingomyelinase","volume":"278","author":"He","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.chroma.2016.12.033_bib0170","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1126\/science.1162395","article-title":"Bioactive contaminants leach from disposable laboratory plasticware","volume":"322","author":"McDonald","year":"2008","journal-title":"Science"},{"key":"10.1016\/j.chroma.2016.12.033_bib0175","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1373\/clinchem.2005.058776","article-title":"Effect of sample collection tubes on cerebrospinal fluid concentrations of tau proteins and amyloid beta peptides","volume":"52","author":"Lewczuk","year":"2006","journal-title":"Clin. Chem."},{"key":"10.1016\/j.chroma.2016.12.033_bib0180","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/0003-2697(83)90738-8","article-title":"How to prevent losses of protein by adsorption to glass and plastic","volume":"135","author":"Suelter","year":"1983","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.chroma.2016.12.033_bib0185","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.ab.2011.02.009","article-title":"The importance of using the optimal plasticware and glassware in studies involving peptides","volume":"414","author":"Goebel-Stengel","year":"2011","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.chroma.2016.12.033_bib0190","first-page":"109","article-title":"Potential role of acid sphingomyelinase in environmental health","volume":"37","author":"He","year":"2012","journal-title":"Zhong Nan Da Bao Yi Xue Ban"}],"container-title":["Journal of Chromatography A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002196731631665X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S002196731631665X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,7,17]],"date-time":"2022-07-17T05:47:16Z","timestamp":1658036836000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S002196731631665X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1]]},"references-count":38,"alternative-id":["S002196731631665X"],"URL":"https:\/\/doi.org\/10.1016\/j.chroma.2016.12.033","relation":{},"ISSN":["0021-9673"],"issn-type":[{"value":"0021-9673","type":"print"}],"subject":[],"published":{"date-parts":[[2017,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Assay to measure sphingomyelinase and ceramidase activities efficiently and safely","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography A","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chroma.2016.12.033","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}