{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T04:47:22Z","timestamp":1775537242203,"version":"3.50.1"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21272209"],"award-info":[{"award-number":["21272209"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81102501"],"award-info":[{"award-number":["81102501"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY14H310007"],"award-info":[{"award-number":["LY14H310007"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography A"],"published-print":{"date-parts":[[2017,3]]},"DOI":"10.1016\/j.chroma.2017.02.022","type":"journal-article","created":{"date-parts":[[2017,2,16]],"date-time":"2017-02-16T09:46:33Z","timestamp":1487238393000},"page":"115-125","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":28,"special_numbering":"C","title":["K-targeted strategy for isolation of phenolic alkaloids of Nelumbo nucifera Gaertn by counter-current chromatography using lysine as a pH regulator"],"prefix":"10.1016","volume":"1490","author":[{"given":"Yanyan","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lihong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuyun","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shihua","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.chroma.2017.02.022_bib0005","doi-asserted-by":"crossref","first-page":"4206","DOI":"10.1016\/j.chroma.2008.10.071","article-title":"Countercurrent chromatography: people and applications","volume":"1216","author":"Berthod","year":"2009","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2017.02.022_bib0010","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1021\/np800144q","article-title":"Countercurrent separation of natural products","volume":"71","author":"Pauli","year":"2008","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.chroma.2017.02.022_bib0015","doi-asserted-by":"crossref","first-page":"3371","DOI":"10.1021\/ac062397g","article-title":"Elution-extrusion countercurrent chromatography: theory and concepts in metabolic analysis","volume":"79","author":"Berthod","year":"2007","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2017.02.022_bib0020","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1126\/science.167.3916.281","article-title":"Countercurrent chromatography. Liquid-liquid partition chromatography without solid support","volume":"167","author":"Ito","year":"1970","journal-title":"Science"},{"key":"10.1016\/j.chroma.2017.02.022_bib0025","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.chroma.2004.12.044","article-title":"Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography","volume":"1065","author":"Ito","year":"2005","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2017.02.022_bib0030","doi-asserted-by":"crossref","first-page":"2545","DOI":"10.1002\/jssc.201000857","article-title":"Separation of alkaloids from herbs using high-speed counter-current chromatography","volume":"34","author":"Fang","year":"2011","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.chroma.2017.02.022_bib0035","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1016\/j.chroma.2008.11.095","article-title":"Role of counter-current chromatography in the modernisation of Chinese herbal medicines","volume":"1216","author":"Sutherland","year":"2009","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2017.02.022_bib0040","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1039\/b700306b","article-title":"Natural product isolation","volume":"25","author":"Sticher","year":"2008","journal-title":"Nat. Prod. Rep."},{"key":"10.1016\/j.chroma.2017.02.022_bib0045","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1021\/np501065h","article-title":"Countercurrent separation of natural products: an update","volume":"78","author":"Friesen","year":"2015","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.chroma.2017.02.022_bib0050","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1093\/chromsci\/8.6.315","article-title":"Countercurrent chromatography \u2212 liquid-liquid partition chromatography without solid support","volume":"8","author":"Ito","year":"1970","journal-title":"J. Chromatogr. Sci."},{"key":"10.1016\/j.chroma.2017.02.022_bib0055","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1080\/10826070701277067","article-title":"Countercurrent chromatography and the journal of liquid chromatography: a love story","volume":"30","author":"Berthod","year":"2007","journal-title":"J. Liq. Chromatogr. Relat. Technol."},{"key":"10.1016\/j.chroma.2017.02.022_bib0060","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0021-9673(00)00811-6","article-title":"Optimisation and characterisation of silica-based reversed-phase liquid chromatographic systems for the analysis of basic pharmaceuticals","volume":"897","author":"Vervoort","year":"2000","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2017.02.022_bib0065","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0021-9673(01)88600-3","article-title":"Comparison of silica-based cyanopropyl and octyl reversed- phase packings for the separation of peptides and proteins","volume":"548","author":"Zhou","year":"1991","journal-title":"J. Chromatogr."},{"key":"10.1016\/j.chroma.2017.02.022_bib0070","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S0021-9673(01)87436-7","article-title":"Separation of flavivirus membrane and capsid proteins by multistep high-performance liquid- chromatography optimized by immunological monitoring","volume":"326","author":"Winkler","year":"1985","journal-title":"J. Chromatogr."},{"key":"10.1016\/j.chroma.2017.02.022_bib0075","first-page":"614","article-title":"Why do peaks tail?","volume":"21","author":"Dolan","year":"2003","journal-title":"Lc Gc North Am."},{"key":"10.1016\/j.chroma.2017.02.022_bib0080","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/0021-9673(93)80809-M","article-title":"The influence of silica structure on reversed-phase retention","volume":"656","author":"Cox","year":"1993","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2017.02.022_bib0085","doi-asserted-by":"crossref","first-page":"6024","DOI":"10.1016\/j.chroma.2010.12.027","article-title":"Distribution ratio: distribution constant and partition coefficient. Countercurrent chromatography retention of benzoic acid","volume":"1218","author":"Berthod","year":"2011","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2017.02.022_bib0090","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/S0021-9673(03)00534-X","article-title":"pH dependence of the hydrophobicity of beta-blocker amine compounds measured by counter-current chromatography","volume":"995","author":"Carda-Broch","year":"2003","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2017.02.022_bib0095","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1021\/ac9810563","article-title":"Hydrophobicity of ionizable compounds. A theoretical study and measurements of diuretic octanol-water partition coefficients by countercurrent chromatography","volume":"71","author":"Berthod","year":"1999","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2017.02.022_bib0100","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.chroma.2007.11.112","article-title":"pH-gradient counter-current chromatography isolation of natural antioxidant chlorogenic acid from Lonicera japonica Thumb using an upright coil planet centrifuge with three multi-layer coils connected in series","volume":"1180","author":"Wang","year":"2008","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2017.02.022_bib0105","doi-asserted-by":"crossref","first-page":"1746","DOI":"10.1002\/jssc.200900872","article-title":"Semi-preparative high-speed counter-current chromatography separation of alkaloids from embryo of the seed of Nelumbo nucifera Gaertn by pH-gradient elution","volume":"33","author":"Duanmu","year":"2010","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.chroma.2017.02.022_bib0110","article-title":"pH-Zone-refining countercurrent chromatography: an efficient preparative separation technique for natural products","volume":"230","author":"Ito","year":"2005","journal-title":"Abstr. Pap. Am. Chem. Soc."},{"key":"10.1016\/j.chroma.2017.02.022_bib0115","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.chroma.2012.11.024","article-title":"pH-Zone-refining counter-current chromatography: origin mechanism, procedure and applications","volume":"1271","author":"Ito","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2017.02.022_bib0120","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1002\/jssc.200900561","article-title":"Preparative separation of alkaloids from Nelumbo nucifera Gaertn by pH-zone-refining counter-current chromatography","volume":"33","author":"Wang","year":"2010","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.chroma.2017.02.022_bib0125","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.chroma.2015.04.043","article-title":"A novel 9\u00d79 map-based solvent selection strategy for targeted counter-current chromatography isolation of natural products","volume":"1400","author":"Liang","year":"2015","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2017.02.022_bib0130","doi-asserted-by":"crossref","first-page":"2777","DOI":"10.1080\/10826070500225234","article-title":"GUESS \u2212 a generally useful estimate of solvent systems in CCC","volume":"28","author":"Friesen","year":"2005","journal-title":"J. Liq. Chromatogr. R. T."},{"key":"10.1016\/j.chroma.2017.02.022_bib0135","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1055\/s-0035-1556277","article-title":"K-targeted isolation of C-glycosylflavones from Vitex agnus-castus by countercurrent methodology","volume":"81","author":"Kulakowski","year":"2015","journal-title":"Planta Med."},{"key":"10.1016\/j.chroma.2017.02.022_bib0140","article-title":"Isoliensinine induces apoptosis in triple-negative human breast cancer cells through ROS generation and p38 MAPK\/JNK activation","volume":"5","author":"Zhang","year":"2015","journal-title":"Sci. Rep."},{"key":"10.1016\/j.chroma.2017.02.022_bib0145","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.jep.2005.09.025","article-title":"Antioxidant activity of Nelumbo nucifera (sacred lotus) seeds","volume":"104","author":"Rai","year":"2006","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.chroma.2017.02.022_bib0150","doi-asserted-by":"crossref","first-page":"e81971","DOI":"10.1371\/journal.pone.0081971","article-title":"Identification and comparison of anti-Inflammatory ingredients from different organs of Lotus Nelumbo by UPLC\/Q-TOF and PCA coupled with a NF-kappa B reporter gene assay","volume":"8","author":"Zhou","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.chroma.2017.02.022_bib0155","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.thromres.2013.05.018","article-title":"Neferine exerts its antithrombotic effect by inhibiting platelet aggregation and promoting dissociation of platelet aggregates","volume":"132","author":"Zhou","year":"2013","journal-title":"Thromb. Res."},{"key":"10.1016\/j.chroma.2017.02.022_bib0160","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.jpba.2006.06.016","article-title":"Separation identification and rapid determination of liensine, isoliensinine and neferine from embryo of the seed of Nelumbo nucifera GAERTN. by liquid chromatography coupled to diode array detector and tandem mass spectrometry","volume":"43","author":"Chen","year":"2007","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.chroma.2017.02.022_bib0165","doi-asserted-by":"crossref","first-page":"2476","DOI":"10.1002\/jssc.200800766","article-title":"Preparative separation and purification of liensinine, isolliensinine and neferine from seed embryo of Nelumbo nucifera GAERTN using high-speed counter-current chromatography","volume":"32","author":"Liu","year":"2009","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.chroma.2017.02.022_bib0170","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.chroma.2004.01.001","article-title":"Determination of liquid-liquid partition coefficients by separation methods","volume":"1037","author":"Berthod","year":"2004","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2017.02.022_bib0175","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.chroma.2004.05.003","article-title":"Preparative counter-current chromatography isolation of liensinine and its analogues from embryo of the seed of Nelumbo nucifera GAERTN using upright coil planet centrifuge with four multilayer coils connected in series","volume":"1041","author":"Wu","year":"2004","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2017.02.022_bib0180","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.chroma.2013.02.079","article-title":"Conical coils counter-current chromatography for preparative isolation and purification of tanshinones from Salvia miltiorrhiza Bunge","volume":"1288","author":"Liang","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2017.02.022_bib0185","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0021-9673(98)00121-6","article-title":"Counter-current chromatography: instrumentation: solvent selection and some recent applications to natural product purification","volume":"808","author":"Foucault","year":"1998","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2017.02.022_bib0190","article-title":"Centrifugal partition chromatography","volume":"68","author":"Margraff","year":"1995"},{"key":"10.1016\/j.chroma.2017.02.022_bib0195","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1021\/jf072415a","article-title":"Performance characteristics of countercurrent separation in analysis of natural products of agricultural significance","volume":"56","author":"Friesent","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.chroma.2017.02.022_bib0200","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1007\/s00216-005-0016-7","article-title":"Alkane effect in the Arizona liquid systems used in countercurrent chromatography","volume":"383","author":"Berthod","year":"2005","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.chroma.2017.02.022_bib0205","doi-asserted-by":"crossref","first-page":"4048","DOI":"10.1021\/ac9002547","article-title":"Screening of complex natural extracts by countercurrent chromatography using a parallel protocol","volume":"81","author":"Lu","year":"2009","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2017.02.022_bib0210","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1080\/10826070601077252","article-title":"Minimising solvent usage in high speed high loading, and high resolution isocratic dynamic extraction","volume":"30","author":"Garrard","year":"2007","journal-title":"J. Liq. Chromatogr. Relat. Technol."},{"key":"10.1016\/j.chroma.2017.02.022_bib0215","doi-asserted-by":"crossref","first-page":"2120","DOI":"10.1002\/rcm.3070","volume":"21","author":"Zhou","year":"2007","journal-title":"Rapid Commun. Mass Spectrom."}],"container-title":["Journal of Chromatography A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967317302455?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967317302455?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,6,15]],"date-time":"2022-06-15T22:33:37Z","timestamp":1655332417000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021967317302455"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3]]},"references-count":43,"alternative-id":["S0021967317302455"],"URL":"https:\/\/doi.org\/10.1016\/j.chroma.2017.02.022","relation":{},"ISSN":["0021-9673"],"issn-type":[{"value":"0021-9673","type":"print"}],"subject":[],"published":{"date-parts":[[2017,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"K-targeted strategy for isolation of phenolic alkaloids of Nelumbo nucifera Gaertn by counter-current chromatography using lysine as a pH regulator","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography A","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chroma.2017.02.022","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}