{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:05:27Z","timestamp":1792065927019,"version":"4.4.0"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Corvus Foundation, Zurich"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography A"],"published-print":{"date-parts":[[2018,7]]},"DOI":"10.1016\/j.chroma.2018.05.013","type":"journal-article","created":{"date-parts":[[2018,5,7]],"date-time":"2018-05-07T12:51:38Z","timestamp":1525697498000},"page":"35-44","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":15,"special_numbering":"C","title":["Development and validation of an ultrahigh performance liquid chromatography-high resolution tandem mass spectrometry assay for nine toxic alkaloids from endophyte-infected pasture grasses in horse serum"],"prefix":"10.1016","volume":"1560","author":[{"given":"Wiebke","family":"Rudolph","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Remane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk K.","family":"Wissenbach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1951-5664","authenticated-orcid":false,"given":"Frank T.","family":"Peters","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"Suppl. 4","key":"10.1016\/j.chroma.2018.05.013_bib0005","doi-asserted-by":"crossref","first-page":"S99","DOI":"10.1086\/342161","article-title":"Evolutionary origins and ecological consequences of endophyte symbiosis with grasses","volume":"160","author":"Clay","year":"2002","journal-title":"Am. Nat."},{"key":"10.1016\/j.chroma.2018.05.013_bib0010","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.biocontrol.2008.01.023","article-title":"Clavicipitaceous endophytes: their ability to enhance resistance of grasses to multiple stresses","volume":"46","author":"Kuldau","year":"2008","journal-title":"Biol. Control"},{"key":"10.1016\/j.chroma.2018.05.013_bib0015","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.2527\/jas.2010-3478","article-title":"Board-invited review: St. Anthony's Fire in livestock: causes, mechanisms, and potential solutions","volume":"89","author":"Strickland","year":"2011","journal-title":"J. Anim. Sci."},{"key":"10.1016\/j.chroma.2018.05.013_bib0020","series-title":"Phytochemicals, Plant Growth, and the Environment","article-title":"Endophyte mycotoxins in animal health","author":"Craig","year":"2012"},{"key":"10.1016\/j.chroma.2018.05.013_bib0025","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1002\/nt.2620010304","article-title":"Alkaloid toxins in endophyte-infected grasses","volume":"1","author":"Powell","year":"1999","journal-title":"Nat. Toxins"},{"key":"10.1016\/j.chroma.2018.05.013_bib0030","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1111\/j.1751-0813.2009.00519.x","article-title":"Fescue-associated oedema of horses grazing on endophyte-inoculated tall fescue grass (Festuca arundinacea) pastures","volume":"87","author":"Bourke","year":"2009","journal-title":"Aust. Vet. J."},{"key":"10.1016\/j.chroma.2018.05.013_bib0035","doi-asserted-by":"crossref","first-page":"899","DOI":"10.2527\/1995.733899x","article-title":"Equine fescue toxicosis: signs and solutions","volume":"73","author":"Cross","year":"1995","journal-title":"J. Anim. Sci."},{"key":"10.1016\/j.chroma.2018.05.013_bib0040","series-title":"Agronomy Monography","doi-asserted-by":"crossref","DOI":"10.2134\/agronmonogr53.c12","article-title":"Physiological basis of fescue toxicosis","author":"Strickland","year":"2009"},{"key":"10.1016\/j.chroma.2018.05.013_bib0045","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1080\/00480169.2011.644189","article-title":"Excretion of loline alkaloids in urine and faeces of sheep dosed with meadow fescue (Festuca pratensis) seed containing high concentrations of loline alkaloids","volume":"60","author":"Gooneratne","year":"2012","journal-title":"N. Z. Vet. J."},{"key":"10.1016\/j.chroma.2018.05.013_bib0050","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.2527\/jas.2007-0576","article-title":"Effects of selected combinations of tall fescue alkaloids on the vasoconstrictive capacity of fescue-naive bovine lateral saphenous veins","volume":"86","author":"Klotz","year":"2008","journal-title":"J. Anim. Sci."},{"key":"10.1016\/j.chroma.2018.05.013_bib0055","doi-asserted-by":"crossref","DOI":"10.3390\/toxins8020047","article-title":"Lolitrem B and indole diterpene alkaloids produced by endophytic fungi of the genus epichloe and their toxic effects in livestock","volume":"8","author":"Philippe","year":"2016","journal-title":"Toxins (Basel)"},{"key":"10.1016\/j.chroma.2018.05.013_bib0060","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1124\/jpet.108.143933","article-title":"The molecular mechanism of ryegrass staggers, a neurological disorder of K+ channels","volume":"327","author":"Imlach","year":"2008","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"10.1016\/j.chroma.2018.05.013_bib0065","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1111\/j.2042-3306.2011.00439.x","article-title":"Clinical expression of lolitrem B (perennial ryegrass) intoxication in horses","volume":"44","author":"Johnstone","year":"2012","journal-title":"Equine Vet. J."},{"key":"10.1016\/j.chroma.2018.05.013_bib0070","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1051\/vetres:2001142","article-title":"Toxicokinetics of ergovaline in the horse after an intravenous administration","volume":"32","author":"Bony","year":"2001","journal-title":"Vet. Res."},{"key":"10.1016\/j.chroma.2018.05.013_bib0075","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1177\/104063879400600311","article-title":"Improved extraction and HPLC methods for ergovaline from plant material and rumen fluid","volume":"6","author":"Craig","year":"1994","journal-title":"J. Vet. Diagn. Invest."},{"key":"10.1016\/j.chroma.2018.05.013_bib0080","doi-asserted-by":"crossref","first-page":"1402","DOI":"10.1080\/19440049.2013.790090","article-title":"Toxicological evaluation and bioaccumulation potential of lolitrem B, endophyte mycotoxin in Japanese black steers","volume":"30","author":"Shimada","year":"2013","journal-title":"Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess."},{"key":"10.1016\/j.chroma.2018.05.013_bib0085","doi-asserted-by":"crossref","first-page":"7213","DOI":"10.1021\/jf901086q","article-title":"Development and validation of an LC-MS method for quantitation of ergot alkaloids in lateral saphenous vein tissue","volume":"57","author":"Smith","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.chroma.2018.05.013_bib0090","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1248\/cpb.39.964","article-title":"Detection and identification of loline and its analogues in horse urine","volume":"39","author":"Takeda","year":"1991","journal-title":"Chem. Pharm. Bull. (Tokyo)"},{"key":"10.1016\/j.chroma.2018.05.013_bib0095","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.phytochem.2006.10.012","article-title":"Peramine and other fungal alkaloids are exuded in the guttation fluid of endophyte-infected grasses","volume":"68","author":"Koulman","year":"2007","journal-title":"Phytochemistry"},{"key":"10.1016\/j.chroma.2018.05.013_bib0100","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1021\/jf9605759","article-title":"Method of analysis for ergovaline in tall fescue by high-performance liquid chromatography","volume":"45","author":"Shelby","year":"1997","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.chroma.2018.05.013_bib0105","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1021\/jf950425i","article-title":"HPLC method for detection of ergotamine, ergosine, and ergine after intravenous injection of a single dose","volume":"44","author":"Moubarak","year":"1996","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.chroma.2018.05.013_bib0110","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1177\/104063879300500216","article-title":"An HPLC method for the detection of ergot in ground and pelleted feeds","volume":"5","author":"Rottinghaus","year":"1993","journal-title":"J. Vet. Diagn. Invest."},{"key":"10.1016\/j.chroma.2018.05.013_bib0115","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0021-9673(01)97668-X","article-title":"High-performance liquid chromatography with stop-flow ultraviolet spectral characterization of lolitrem neurotoxins from perennial ryegrass","volume":"322","author":"Gallagher","year":"1985","journal-title":"J. Chromatogr."},{"key":"10.1016\/j.chroma.2018.05.013_bib0120","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0021-9673(98)00002-8","article-title":"Rapid analysis of ergovaline in ovine plasma using high-performance liquid chromatography with fluorimetric detection","volume":"815","author":"Jaussaud","year":"1998","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2018.05.013_bib0125","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0378-4347(99)00166-8","article-title":"Analysis of ergovaline in milk using high-performance liquid chromatography with fluorimetric detection","volume":"729","author":"Durix","year":"1999","journal-title":"J. Chromatogr. B Biomed. Sci. Appl."},{"key":"10.1016\/j.chroma.2018.05.013_bib0130","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1177\/104063870601800408","article-title":"Detection of lysergic acid in ruminal fluid, urine, and in endophyte-infected tall fescue using high-performance liquid chromatography","volume":"18","author":"Lodge-Ivey","year":"2006","journal-title":"J. Vet. Diagn. Invest."},{"key":"10.1016\/j.chroma.2018.05.013_bib0135","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/s00216-010-4398-9","article-title":"Development of the first metabolite-based LC-MS(n) urine drug screening procedure-exemplified for antidepressants","volume":"400","author":"Wissenbach","year":"2011","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.chroma.2018.05.013_bib0140","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.forsciint.2006.05.021","article-title":"Validation of new methods","volume":"165","author":"Peters","year":"2007","journal-title":"Forensic Sci. Int."},{"key":"10.1016\/j.chroma.2018.05.013_bib0145","first-page":"185","article-title":"Anhang B zur Richtilinie der GTFCh zur Qualit\u201etssicherung bei forensisch-toxikologischen Untersuchungen \u2013 Anforderungen an die Validierung von Analysenmethoden","volume":"76","author":"Peters","year":"2009","journal-title":"Toxichem Krimtech"},{"key":"10.1016\/j.chroma.2018.05.013_bib0150","series-title":"Mass Spectral Library of Drugs, Poisons, Pesticides, Pollutants and Their Metabolites 2011","author":"Maurer","year":"2011"},{"key":"10.1016\/j.chroma.2018.05.013_bib0155","series-title":"LC-MSn Library of Drugs, Poisons and Their Metabolites","author":"Maurer","year":"2014"},{"key":"10.1016\/j.chroma.2018.05.013_bib0160","doi-asserted-by":"crossref","first-page":"3019","DOI":"10.1021\/ac020361s","article-title":"Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS\/MS","volume":"75","author":"Matuszewski","year":"2003","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2018.05.013_bib0165","doi-asserted-by":"crossref","first-page":"5856","DOI":"10.1021\/jf025602b","article-title":"Simplified extraction of ergovaline and peramine for analysis of tissue distribution in endophyte-infected grass tillers","volume":"50","author":"Spiering","year":"2002","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.chroma.2018.05.013_bib0170","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1021\/jf00080a023","article-title":"Analysis of ergopeptine alkaloids in endophyte-infected tall fescue","volume":"36","author":"Yates","year":"1988","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.chroma.2018.05.013_bib0175","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1177\/104063870101300307","article-title":"Semiquantitative determination of ergot alkaloids in seed, straw, and digesta samples using a competitive enzyme-linked immunosorbent assay","volume":"13","author":"Schnitzius","year":"2001","journal-title":"J. Vet. Diagn. Invest."},{"key":"10.1016\/j.chroma.2018.05.013_bib0180","doi-asserted-by":"crossref","first-page":"1651","DOI":"10.1002\/rcm.3005","article-title":"Ergot alkaloids in Norwegian wild grasses: a mass spectrometric approach","volume":"21","author":"Uhlig","year":"2007","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"10.1016\/j.chroma.2018.05.013_bib0185","doi-asserted-by":"crossref","first-page":"4674","DOI":"10.1021\/jf970528w","article-title":"Analysis of ergot alkaloids in endophyte-infected tall fescue by liquid chromatography electrospray ionization mass spectrometry","volume":"45","author":"Shelby","year":"1997","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.chroma.2018.05.013_bib0190","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1021\/jf00091a040","article-title":"Analysis of loline alkaloids in endophyte-infected tall fescue by capillary gas-Chromatography","volume":"38","author":"Yates","year":"1990","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.chroma.2018.05.013_bib0195","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1080\/00480169.2011.634746","article-title":"The evaluation of endophyte toxin residues in sheep fat","volume":"60","author":"Finch","year":"2012","journal-title":"N. Z. Vet. J."},{"key":"10.1016\/j.chroma.2018.05.013_bib0200","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1016\/j.trac.2008.08.013","article-title":"Use of recovery and bias information in analytical chemistry and estimation of its uncertainty contribution","volume":"27","author":"Linsinger","year":"2008","journal-title":"Trac-Trend Anal. Chem."},{"key":"10.1016\/j.chroma.2018.05.013_bib0205","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/0167-8809(93)90040-V","article-title":"Chemistry, occurrence and biological effects of saturated pyrrolizidine alkaloids associated with endophyte grass interactions","volume":"44","author":"Bush","year":"1993","journal-title":"Agr. Ecosyst. Environ."},{"key":"10.1016\/j.chroma.2018.05.013_bib0210","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1016\/j.phytochem.2007.01.010","article-title":"Loline alkaloids: currencies of mutualism","volume":"68","author":"Schardl","year":"2007","journal-title":"Phytochemistry"},{"key":"10.1016\/j.chroma.2018.05.013_bib0215","article-title":"Development and validation of a UHPLC-HRMS\/MS quantification method for hypoglycin a and MCPA-carnitine in horse serum in cases of atypical myopathy","author":"Rudolph","year":"2017","journal-title":"Drug Test. Anal."},{"key":"10.1016\/j.chroma.2018.05.013_bib0220","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/0378-4347(92)80526-V","article-title":"Systematic toxicological analysis of drugs and their metabolites by gas chromatography-mass spectrometry","volume":"580","author":"Maurer","year":"1992","journal-title":"J. Chromatogr."},{"key":"10.1016\/j.chroma.2018.05.013_bib0225","series-title":"Mass Spectral and GC Data of Drugs, Poisons, Pesticides, Pollutants and Their Metabolites","author":"Maurer","year":"2011"},{"key":"10.1016\/j.chroma.2018.05.013_bib0230","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.aca.2015.08.018","article-title":"Orbitrap technology for comprehensive metabolite-based liquid chromatographic-high resolution-tandem mass spectrometric urine drug screening \u2013 exemplified for cardiovascular drugs","volume":"891","author":"Helfer","year":"2015","journal-title":"Anal. Chim. Acta"}],"container-title":["Journal of Chromatography A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967318305739?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967318305739?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,6,21]],"date-time":"2019-06-21T00:01:22Z","timestamp":1561075282000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021967318305739"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7]]},"references-count":46,"alternative-id":["S0021967318305739"],"URL":"https:\/\/doi.org\/10.1016\/j.chroma.2018.05.013","relation":{},"ISSN":["0021-9673"],"issn-type":[{"value":"0021-9673","type":"print"}],"subject":[],"published":{"date-parts":[[2018,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and validation of an ultrahigh performance liquid chromatography-high resolution tandem mass spectrometry assay for nine toxic alkaloids from endophyte-infected pasture grasses in horse serum","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography A","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chroma.2018.05.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}