{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T05:04:50Z","timestamp":1785128690118,"version":"3.55.0"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T00:00:00Z","timestamp":1665619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography A"],"published-print":{"date-parts":[[2022,11]]},"DOI":"10.1016\/j.chroma.2022.463566","type":"journal-article","created":{"date-parts":[[2022,10,14]],"date-time":"2022-10-14T06:15:24Z","timestamp":1665728124000},"page":"463566","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["Determination of eight phosphatidylethanol homologues in blood by reversed phase liquid chromatography\u2013tandem mass spectrometry \u2013 How to avoid co-elution of phosphatidylethanols and unwanted phospholipids"],"prefix":"10.1016","volume":"1684","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5171-3877","authenticated-orcid":false,"given":"Marisa Henriques","family":"Maria","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5917-8776","authenticated-orcid":false,"given":"Benedicte Marie","family":"J\u00f8rgenrud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7675-2095","authenticated-orcid":false,"given":"Thomas","family":"Berg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.chroma.2022.463566_bib0001","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1186\/1471-2458-12-734","article-title":"Alcohol, psychoactive substances and non-fatal road traffic accidents\u2013a case-control study","volume":"12","author":"Bogstrand","year":"2012","journal-title":"BMC Public Health"},{"key":"10.1016\/j.chroma.2022.463566_bib0002","doi-asserted-by":"crossref","DOI":"10.1016\/j.drugalcdep.2019.107588","article-title":"Harmful alcohol use among acutely ill hospitalized medical patients in Oslo and Moscow: a cross-sectional study","volume":"204","author":"Kabashi","year":"2019","journal-title":"Drug Alcohol Depend."},{"issue":"2","key":"10.1016\/j.chroma.2022.463566_bib0003","first-page":"135","article-title":"The risks associated with alcohol use and alcoholism","volume":"34","author":"Rehm","year":"2011","journal-title":"Alcohol Res. Health"},{"issue":"9682","key":"10.1016\/j.chroma.2022.463566_bib0004","doi-asserted-by":"crossref","first-page":"2223","DOI":"10.1016\/S0140-6736(09)60746-7","article-title":"Global burden of disease and injury and economic cost attributable to alcohol use and alcohol-use disorders","volume":"373","author":"Rehm","year":"2009","journal-title":"Lancet"},{"key":"10.1016\/j.chroma.2022.463566_bib0005","series-title":"Global Status Report on Alcohol and Health","year":"2014"},{"issue":"7","key":"10.1016\/j.chroma.2022.463566_bib0006","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1007\/s40273-021-01031-8","article-title":"What are the economic costs to society attributable to alcohol use? A systematic review and modelling study","volume":"39","author":"Manthey","year":"2021","journal-title":"Pharmacoeconomics"},{"issue":"2","key":"10.1016\/j.chroma.2022.463566_bib0007","first-page":"87","article-title":"Measuring costs of alcohol harm to others: a review of the literature","volume":"114","author":"Navarro","year":"2011","journal-title":"Drug Alcohol Depend."},{"issue":"5","key":"10.1016\/j.chroma.2022.463566_bib0008","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.amepre.2011.06.045","article-title":"Economic costs of excessive alcohol consumption in the US, 2006","volume":"41","author":"Bouchery","year":"2011","journal-title":"Am. J. Prev. Med."},{"issue":"4","key":"10.1016\/j.chroma.2022.463566_bib0009","first-page":"391","article-title":"ESBRA 1994 award lecture. phosphatidylethanol formation: specific effects of ethanol mediated via phospholipase D","volume":"30","author":"Gustavsson","year":"1995","journal-title":"Alcohol Alcohol."},{"issue":"6","key":"10.1016\/j.chroma.2022.463566_bib0010","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1111\/acer.13062","article-title":"Characterization of the pharmacokinetics of phosphatidylethanol 16:0\/18:1 and 16:0\/18:2 in human whole blood after alcohol consumption in a clinical laboratory study","volume":"40","author":"Javors","year":"2016","journal-title":"Alcohol Clin. Exp. Res."},{"issue":"1","key":"10.1016\/j.chroma.2022.463566_bib0011","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/0014-5793(83)80474-8","article-title":"An abnormal phospholipid in rat organs after ethanol treatment","volume":"152","author":"Alling","year":"1983","journal-title":"FEBS Lett."},{"issue":"1","key":"10.1016\/j.chroma.2022.463566_bib0012","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/0005-2760(84)90060-2","article-title":"Phosphatidylethanol formation in rat organs after ethanol treatment","volume":"793","author":"Aradottir","year":"1984","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.chroma.2022.463566_bib0013","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.cca.2012.11.014","article-title":"Non-oxidative ethanol metabolites as a measure of alcohol intake","volume":"415","author":"Maenhout","year":"2013","journal-title":"Clin. Chim. Acta"},{"issue":"18","key":"10.1016\/j.chroma.2022.463566_bib0014","first-page":"309","article-title":"Alcohol biomarkers in clinical and forensic contexts","volume":"115","author":"Andresen-Streichert","year":"2018","journal-title":"Dtsch. Arztebl. Int."},{"issue":"4","key":"10.1016\/j.chroma.2022.463566_bib0015","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1093\/alcalc\/agl027","article-title":"PHosphatidylethanol (PEth) concentrations in blood are correlated to reported alcohol intake in alcohol-dependent patients","volume":"41","author":"Aradottir","year":"2006","journal-title":"Alcohol Alcohol."},{"issue":"1","key":"10.1016\/j.chroma.2022.463566_bib0016","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1111\/j.1369-1600.2009.00185.x","article-title":"Phosphatidylethanol: normalization during detoxification, gender aspects and correlation with other biomarkers and self-reports","volume":"15","author":"Wurst","year":"2010","journal-title":"Addict. Biol."},{"issue":"46","key":"10.1016\/j.chroma.2022.463566_bib0017","doi-asserted-by":"crossref","first-page":"8423","DOI":"10.1016\/j.chroma.2011.09.068","article-title":"Quantitative profiling of phosphatidylethanol molecular species in human blood by liquid chromatography high resolution mass spectrometry","volume":"1218","author":"Nalesso","year":"2011","journal-title":"J. Chromatogr. A"},{"issue":"15","key":"10.1016\/j.chroma.2022.463566_bib0018","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.1016\/j.cca.2011.04.022","article-title":"Method development for routine liquid chromatography-mass spectrometry measurement of the alcohol biomarker phosphatidylethanol (PEth) in blood","volume":"412","author":"Zheng","year":"2011","journal-title":"Clin. Chim. Acta"},{"issue":"11","key":"10.1016\/j.chroma.2022.463566_bib0019","doi-asserted-by":"crossref","first-page":"2094","DOI":"10.1111\/acer.13865","article-title":"Pharmacokinetics of phosphatidylethanol 16:0\/20:4 in human blood after alcohol intake","volume":"42","author":"Lopez-Cruzan","year":"2018","journal-title":"Alcohol Clin. Exp. Res."},{"issue":"6","key":"10.1016\/j.chroma.2022.463566_bib0020","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1093\/alcalc\/agz064","article-title":"Dose-response characteristics of the alcohol biomarker phosphatidylethanol (PEth)-a study of outpatients in treatment for reduced drinking","volume":"54","author":"Helander","year":"2019","journal-title":"Alcohol Alcohol."},{"issue":"7","key":"10.1016\/j.chroma.2022.463566_bib0021","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1007\/s00216-010-3458-5","article-title":"Identification of 48 homologues of phosphatidylethanol in blood by LC-ESI-MS\/MS","volume":"396","author":"Gnann","year":"2010","journal-title":"Anal. Bioanal. Chem."},{"issue":"2","key":"10.1016\/j.chroma.2022.463566_bib0022","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.jpba.2007.02.006","article-title":"Recent advances in high-throughput quantitative bioanalysis by LC-MS\/MS","volume":"44","author":"Xu","year":"2007","journal-title":"J. Pharm. Biomed. Anal."},{"issue":"1","key":"10.1016\/j.chroma.2022.463566_bib0023","doi-asserted-by":"crossref","first-page":"91","DOI":"10.4155\/bio.12.295","article-title":"A systematic approach for developing a robust LC-MS\/MS method for bioanalysis","volume":"5","author":"Meng","year":"2013","journal-title":"Bioanalysis"},{"key":"10.1016\/j.chroma.2022.463566_bib0024","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.chroma.2012.08.087","article-title":"Challenges in the development of bioanalytical liquid chromatography-mass spectrometry method with emphasis on fast analysis","volume":"1292","author":"Novakova","year":"2013","journal-title":"J. Chromatogr. A"},{"issue":"1","key":"10.1016\/j.chroma.2022.463566_bib0025","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.clinbiochem.2010.08.008","article-title":"Recent advances of liquid chromatography-(tandem) mass spectrometry in clinical and forensic toxicology","volume":"44","author":"Peters","year":"2011","journal-title":"Clin. Biochem."},{"issue":"13","key":"10.1016\/j.chroma.2022.463566_bib0026","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1016\/j.clinbiochem.2016.07.010","article-title":"Recent advances of liquid chromatography-(tandem) mass spectrometry in clinical and forensic toxicology - an update","volume":"49","author":"Remane","year":"2016","journal-title":"Clin. Biochem."},{"issue":"1","key":"10.1016\/j.chroma.2022.463566_bib0027","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1093\/jat\/bkx075","article-title":"High throughput UPLC(R)-MSMS method for the analysis of phosphatidylethanol (PEth) 16:0\/18:1, a specific biomarker for alcohol consumption, in whole blood","volume":"42","author":"Andreassen","year":"2018","journal-title":"J. Anal. Toxicol."},{"key":"10.1016\/j.chroma.2022.463566_bib0028","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2022.114635","article-title":"A comparison of an optimized automated sample preparation of PEth in blood pretreated by freezing versus manual preparation in whole blood, analyzing by UHPLC - MS\/MS","volume":"212","author":"Muller","year":"2022","journal-title":"J. Pharm. Biomed. Anal."},{"issue":"3","key":"10.1016\/j.chroma.2022.463566_bib0029","doi-asserted-by":"crossref","first-page":"229","DOI":"10.4155\/bio.15.240","article-title":"Bioanalytical procedures and developments in the determination of alcohol biomarkers in biological specimens","volume":"8","author":"Oppolzer","year":"2016","journal-title":"Bioanalysis"},{"issue":"6","key":"10.1016\/j.chroma.2022.463566_bib0030","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1373\/jalm.2017.024828","article-title":"High-throughput LC-MS\/MS method for determination of the alcohol use biomarker phosphatidylethanol in clinical samples by use of a simple automated extraction procedure-preanalytical and analytical conditions","volume":"2","author":"Isaksson","year":"2018","journal-title":"J. Appl. Lab. Med."},{"issue":"1","key":"10.1016\/j.chroma.2022.463566_bib0031","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1002\/bmc.1373","article-title":"Systematic LC-MS\/MS bioanalytical method development that incorporates plasma phospholipids risk avoidance, usage of incurred sample and well thought-out chromatography","volume":"24","author":"Jemal","year":"2010","journal-title":"Biomed. Chromatogr."},{"issue":"14","key":"10.1016\/j.chroma.2022.463566_bib0032","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.1002\/rcm.4121","volume":"23","author":"Xia","year":"2009","journal-title":"Rapid Commun. Mass Spectrom."},{"issue":"31","key":"10.1016\/j.chroma.2022.463566_bib0033","doi-asserted-by":"crossref","first-page":"3303","DOI":"10.1016\/j.jchromb.2010.10.012","article-title":"Investigation of endogenous blood plasma phospholipids, cholesterol and glycerides that contribute to matrix effects in bioanalysis by liquid chromatography\/mass spectrometry","volume":"878","author":"Ismaiel","year":"2010","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"10.1016\/j.chroma.2022.463566_bib0034","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.jchromb.2012.02.031","article-title":"Systematic evaluation of supported liquid extraction in reducing matrix effect and improving extraction efficiency in LC-MS\/MS based bioanalysis for 10 model pharmaceutical compounds","volume":"891-892","author":"Jiang","year":"2012","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"issue":"1","key":"10.1016\/j.chroma.2022.463566_bib0035","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.aca.2009.10.004","article-title":"A review of current trends and advances in modern bio-analytical methods: chromatography and sample preparation","volume":"656","author":"Novakova","year":"2009","journal-title":"Anal. Chim. Acta"},{"issue":"2","key":"10.1016\/j.chroma.2022.463566_bib0036","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.jchromb.2006.02.011","article-title":"Liquid chromatography-mass spectrometry\/mass spectrometry method development for drug metabolism studies: examining lipid matrix ionization effects in plasma","volume":"833","author":"Little","year":"2006","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"issue":"11","key":"10.1016\/j.chroma.2022.463566_bib0037","first-page":"22","article-title":"Phospholipids of animal and marine origin: structure, function, and anti-inflammatory properties","author":"Lordan","year":"2017","journal-title":"Molecules"},{"issue":"1","key":"10.1016\/j.chroma.2022.463566_bib0038","doi-asserted-by":"crossref","first-page":"e22631","DOI":"10.1002\/jcla.22631","article-title":"Determination of phosphatidylethanol 16:0\/18:1 in whole blood by 96-well supported liquid extraction and UHPLC-MS\/MS","volume":"33","author":"Berg","year":"2019","journal-title":"J. Clin. Lab. Anal."},{"key":"10.1016\/j.chroma.2022.463566_bib0039","doi-asserted-by":"crossref","DOI":"10.1016\/j.vascn.2020.106939","article-title":"Determination of the alcohol biomarker phosphatidylethanol 16:0\/18:1 and 33 compounds from eight different drug classes in whole blood by LC-MS\/MS","volume":"107","author":"Jorgenrud","year":"2021","journal-title":"J. Pharmacol. Toxicol. Methods"},{"issue":"4","key":"10.1016\/j.chroma.2022.463566_bib0040","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.jchromb.2008.12.052","article-title":"Determination of opiates and cocaine in urine by high pH mobile phase reversed phase UPLC-MS\/MS","volume":"877","author":"Berg","year":"2009","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"issue":"9","key":"10.1016\/j.chroma.2022.463566_bib0041","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1093\/chromsci\/bmz061","article-title":"Effect of mobile phase pH on the electrospray ionization efficiency and qualitative analysis of pharmaceuticals in ESI\u00a0+\u00a0LC-MS\/MS","volume":"57","author":"Kafeenah","year":"2019","journal-title":"J. Chromatogr. Sci."},{"issue":"8","key":"10.1016\/j.chroma.2022.463566_bib0042","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.4155\/bio.09.133","article-title":"Effect of high pH mobile phase on the sensitivity of multiple drugs by LC positive electrospray ionization MS\/MS","volume":"1","author":"Mess","year":"2009","journal-title":"Bioanalysis"},{"issue":"1","key":"10.1016\/j.chroma.2022.463566_bib0043","doi-asserted-by":"crossref","first-page":"e4409","DOI":"10.1002\/bmc.4409","article-title":"Use of high-pH (basic\/alkaline) mobile phases for LC-MS or LC-MS\/MS bioanalysis","volume":"33","author":"Tan","year":"2019","journal-title":"Biomed. Chromatogr."},{"issue":"1","key":"10.1016\/j.chroma.2022.463566_bib0044","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0021-9673(00)01012-8","article-title":"Effect of the eluent pH and acidic modifiers in high-performance liquid chromatography retention of basic analytes","volume":"913","author":"LoBrutto","year":"2001","journal-title":"J. Chromatogr. A"}],"container-title":["Journal of Chromatography A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967322007579?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967322007579?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T06:19:46Z","timestamp":1758262786000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021967322007579"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11]]},"references-count":44,"alternative-id":["S0021967322007579"],"URL":"https:\/\/doi.org\/10.1016\/j.chroma.2022.463566","relation":{},"ISSN":["0021-9673"],"issn-type":[{"value":"0021-9673","type":"print"}],"subject":[],"published":{"date-parts":[[2022,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Determination of eight phosphatidylethanol homologues in blood by reversed phase liquid chromatography\u2013tandem mass spectrometry \u2013 How to avoid co-elution of phosphatidylethanols and unwanted phospholipids","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography A","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chroma.2022.463566","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"463566"}}