{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T03:45:01Z","timestamp":1777607101273,"version":"3.51.4"},"reference-count":27,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100022573","name":"Institute of Analytical Chemistry Czech Academy of Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100022573","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["NU23-08-00303"],"award-info":[{"award-number":["NU23-08-00303"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["68081715"],"award-info":[{"award-number":["68081715"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography A"],"published-print":{"date-parts":[[2024,11]]},"DOI":"10.1016\/j.chroma.2024.465354","type":"journal-article","created":{"date-parts":[[2024,9,7]],"date-time":"2024-09-07T06:16:33Z","timestamp":1725689793000},"page":"465354","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Optimization of saliva sampling methods for analysis of bile acids by UHPLC-MS"],"prefix":"10.1016","volume":"1736","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3580-7405","authenticated-orcid":false,"given":"V\u011bra","family":"Dosed\u011blov\u00e1","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark\u00e9ta","family":"La\u0161tovi\u010dkov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0160tefan","family":"Kone\u010dn\u00fd","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u0159\u00ed","family":"Dolina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petr","family":"Kub\u00e1\u0148","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.chroma.2024.465354_bib0001","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1002\/elps.202000139","article-title":"Analysis of bile acids in human biological samples by microcolumn separation techniques: a review","volume":"42","author":"Dosed\u011blov\u00e1","year":"2021","journal-title":"Electrophoresis"},{"key":"10.1016\/j.chroma.2024.465354_bib0002","doi-asserted-by":"crossref","first-page":"7830","DOI":"10.3390\/molecules27227830","article-title":"Bile Acids: physiological Activity and Perspectives of Using in Clinical and Laboratory Diagnostics","volume":"27","author":"Shansky","year":"2022","journal-title":"Molecules."},{"key":"10.1016\/j.chroma.2024.465354_bib0003","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1038\/s41575-023-00883-z","article-title":"Pathophysiology of gastro-oesophageal reflux disease: implications for diagnosis and management","volume":"21","author":"Arg\u00fcero","year":"2024","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"10.1016\/j.chroma.2024.465354_bib0004","first-page":"764","article-title":"Alkaline gastroesophageal reflux \u2013 Implications in the development of complications in Barrett's columnar-lined lower esophagus","volume":"106","author":"Attwood","year":"1989","journal-title":"Surg"},{"key":"10.1016\/j.chroma.2024.465354_bib0005","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/j.gassur.2006.02.005","article-title":"The impact of reflux composition on mucosal injury and esophageal function","volume":"10","author":"Oh","year":"2006","journal-title":"J. Gastrointest. Surg."},{"key":"10.1016\/j.chroma.2024.465354_bib0006","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aca.2019.05.004","article-title":"Capillary electrophoresis of small ions and molecules in less conventional human body fluid samples: a review","volume":"1075","author":"Kub\u00e1\u0148","year":"2019","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.chroma.2024.465354_bib0007","doi-asserted-by":"crossref","first-page":"115781","DOI":"10.1016\/j.trac.2019.115781","article-title":"Saliva sampling: methods and devices, An overview","volume":"124","author":"Bellagambi","year":"2020","journal-title":"Trends Anal. Chem."},{"key":"10.1016\/j.chroma.2024.465354_bib0008","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1111\/coa.13643","article-title":"Impact of bile acids on the severity of laryngo-pharyngeal reflux","volume":"46","author":"De Corso","year":"2021","journal-title":"Clinical Otolaryngology Clin. Otolaryngol."},{"key":"10.1016\/j.chroma.2024.465354_bib0009","first-page":"59","article-title":"Higher levels of total pepsin and bile acids in the saliva as a possible risk factor for early laryngeal cancer","volume":"49","author":"Sereg-Bahar","year":"2015","journal-title":"Radiotherapy and Oncology"},{"key":"10.1016\/j.chroma.2024.465354_bib0010","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1039\/C6AY03243E","article-title":"Detection of total bile acids in biological samples using an indirect competitive ELISA based on four monoclonal antibodies","volume":"9","author":"Liu","year":"2017","journal-title":"Anal. Methods"},{"key":"10.1016\/j.chroma.2024.465354_bib0011","doi-asserted-by":"crossref","first-page":"7799","DOI":"10.1007\/s00216-014-8230-9","article-title":"Quantitative profiling of bile acids in blood, adipose tis-sue, intestine, and gall bladder samples using ultra high performance liquid chromatography-tandem mass spectrometry","volume":"406","author":"J\u00e4ntti","year":"2014","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.chroma.2024.465354_bib0012","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.cca.2016.11.014","article-title":"Bile acid preparation and comprehensive analysis by high performance liquid chromatography\u2013high-resolution mass spectrometry","volume":"464","author":"Amplatz","year":"2017","journal-title":"Clin. Chim. Acta."},{"key":"10.1016\/j.chroma.2024.465354_bib0013","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1093\/chromsci\/bmu167","article-title":"Rapid determination of bile acids in bile from various mammals by reversed-phase ultra-fast liquid chromatography","volume":"53","author":"Si","year":"2015","journal-title":"J. Chromatogr. Sci."},{"key":"10.1016\/j.chroma.2024.465354_bib0014","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2020.461278","article-title":"Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry","volume":"1625","author":"\u010eur\u010d","year":"2020","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2024.465354_bib0015","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2022.463287","article-title":"Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: possible non-invasive diagnostics of Barret\u00b4s esophagus?","volume":"1676","author":"Dosed\u011blov\u00e1","year":"2022","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2024.465354_bib0016","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.steroids.2010.01.015","article-title":"Salivary chenodeoxycholic acid and its glycine- conjugate: their determination method using LC-MS\/MS and variation of their concentrations with increased saliva flow rate","volume":"75","author":"Higashi","year":"2010","journal-title":"Steroids."},{"key":"10.1016\/j.chroma.2024.465354_bib0017","doi-asserted-by":"crossref","first-page":"4293","DOI":"10.1007\/s00216-018-1078-7","article-title":"Facile derivatization of ultratrace carboxylic acids in saliva for quantification by HPLC-MS\/MS","volume":"410","author":"Guo","year":"2018","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.chroma.2024.465354_bib0018","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.jchromb.2012.05.015","article-title":"Bile acid profiling in human biological samples: comparison of extraction procedures and application to normal and cholestatic patients","volume":"899","author":"Humbert","year":"2012","journal-title":"J. Chromatogr. B."},{"key":"10.1016\/j.chroma.2024.465354_bib0019","unstructured":"Salivette saliva sampling systems. https:\/\/www.sarstedt.com\/en\/products\/diagnostic\/salivasputum\/(accessed 26 June 2024)."},{"key":"10.1016\/j.chroma.2024.465354_bib0020","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1136\/gut.2007.146134","article-title":"Gastro-oesophageal reflux and aspiration of gastric contents in adult patients with cystic fibrosis","volume":"57","author":"Blondeau","year":"2008","journal-title":"Gut"},{"key":"10.1016\/j.chroma.2024.465354_bib0021","first-page":"1","article-title":"Pilot study evaluating salivary bile acids as a diagnostic biomarker of laryngopharyngeal reflux","volume":"1","author":"Krause","year":"2024","journal-title":"Diseases of the Esophagus"},{"key":"10.1016\/j.chroma.2024.465354_bib0022","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1002\/jcla.20281","article-title":"Rapid saliva processing techniques for near real-time analysis of salivary steroids and protein","volume":"22","author":"Atkinson","year":"2008","journal-title":"J. Clin. Lab. Anal."},{"key":"10.1016\/j.chroma.2024.465354_bib0023","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.steroids.2018.05.013","article-title":"Testosterone, androstenedione, cortisol and cortisone levels in human unstimulated, stimulated and parotid saliva","volume":"138","author":"B\u00fcttler","year":"2018","journal-title":"Steroids."},{"key":"10.1016\/j.chroma.2024.465354_bib0024","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1016\/j.cca.2012.04.035","article-title":"Saliva collection devices affect sex steroid concentrations","volume":"413","author":"Celec","year":"2012","journal-title":"Clin. Chim. Acta"},{"key":"10.1016\/j.chroma.2024.465354_bib0025","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1016\/j.steroids.2006.09.007","article-title":"Influence of commercial collection devices for saliva on the reliability of salivary steroids analysis","volume":"71","author":"Gr\u00f6schl","year":"2006","journal-title":"Steroids."},{"key":"10.1016\/j.chroma.2024.465354_bib0026","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1136\/gut.19.1.32","article-title":"Physiological factors influencing serum bile acid levels","volume":"19","author":"Ponz de Leon","year":"1978","journal-title":"Gut"},{"key":"10.1016\/j.chroma.2024.465354_bib0027","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1056\/NEJM197410032911401","article-title":"Dynamics of the enterohepatic circulation of bile acids","volume":"291","author":"LaRusso","year":"1974","journal-title":"N. Eng. J. Med."}],"container-title":["Journal of Chromatography A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967324007283?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967324007283?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T21:08:27Z","timestamp":1777410507000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021967324007283"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11]]},"references-count":27,"alternative-id":["S0021967324007283"],"URL":"https:\/\/doi.org\/10.1016\/j.chroma.2024.465354","relation":{},"ISSN":["0021-9673"],"issn-type":[{"value":"0021-9673","type":"print"}],"subject":[],"published":{"date-parts":[[2024,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Optimization of saliva sampling methods for analysis of bile acids by UHPLC-MS","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography A","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chroma.2024.465354","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"465354"}}