{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T03:48:21Z","timestamp":1777607301726,"version":"3.51.4"},"reference-count":61,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Zhejiang Province Natural Science Foundation","doi-asserted-by":"publisher","award":["LD21B060001"],"award-info":[{"award-number":["LD21B060001"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22078296"],"award-info":[{"award-number":["22078296"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21576240"],"award-info":[{"award-number":["21576240"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography A"],"published-print":{"date-parts":[[2025,5]]},"DOI":"10.1016\/j.chroma.2025.465801","type":"journal-article","created":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T02:54:14Z","timestamp":1739933654000},"page":"465801","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Mass production of cation-exchange cryogels and their chromatographic adsorption performance for bioseparation"],"prefix":"10.1016","volume":"1748","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4628-4075","authenticated-orcid":false,"given":"Qining","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenyuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingyan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingguang","family":"Yi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruofei","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linian","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songhong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoling","family":"Lou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5804-6154","authenticated-orcid":false,"given":"Junxian","family":"Yun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.chroma.2025.465801_bib0001","doi-asserted-by":"crossref","first-page":"979","DOI":"10.3390\/gels9120979","article-title":"Cryogel scaffolds for tissue-engineering: advances and challenges for effective bone and cartilage regeneration","volume":"9","author":"Carriero","year":"2023","journal-title":"Gels"},{"key":"10.1016\/j.chroma.2025.465801_bib0002","doi-asserted-by":"crossref","first-page":"12491","DOI":"10.1007\/s00289-023-04683-y","article-title":"Advances in cryostructures and their applications in biomedical and pharmaceutical products","volume":"80","author":"Babanejad","year":"2023","journal-title":"Polym. Bull."},{"key":"10.1016\/j.chroma.2025.465801_bib0003","doi-asserted-by":"crossref","first-page":"7793","DOI":"10.1021\/acs.langmuir.3c00623","article-title":"Biocomposite cryogels for photothermal decontamination of water","volume":"39","author":"Zafar","year":"2023","journal-title":"Langmuir"},{"key":"10.1016\/j.chroma.2025.465801_bib0004","doi-asserted-by":"crossref","DOI":"10.1016\/j.seppur.2023.123678","article-title":"A systematic review on new advancement and assessment of emerging polymeric cryogels for environmental sustainability and energy production","volume":"316","author":"Haleem","year":"2023","journal-title":"Sep. Purif. Technol."},{"key":"10.1016\/j.chroma.2025.465801_bib0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.micres.2024.127656","article-title":"Nanocomposites against Pseudomonas aeruginosa biofilms: recent advances, challenges, and future prospects","volume":"282","author":"Omran","year":"2024","journal-title":"Microbiol. Res."},{"key":"10.1016\/j.chroma.2025.465801_bib0006","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.1002\/pc.24120","article-title":"Graphene oxide embedded p(4-VP) cryogel composites for fast dye removal\/separations","volume":"39","author":"Sahiner","year":"2018","journal-title":"Polym. Compos."},{"key":"10.1016\/j.chroma.2025.465801_bib0007","doi-asserted-by":"crossref","DOI":"10.1002\/jssc.202200638","article-title":"Preparation of composite monoliths of quaternized chitosan and diatom earth for protein separation","volume":"46","author":"Tanaka","year":"2023","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.chroma.2025.465801_bib0008","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1016\/j.snb.2017.02.105","article-title":"Chitosan cryogel with embedded gold nanoparticles decorated multiwalled carbon nanotubes modified electrode for highly sensitive flow based non-enzymatic glucose sensor","volume":"246","author":"Kangkamano","year":"2017","journal-title":"Sens. Actuators B"},{"key":"10.1016\/j.chroma.2025.465801_bib0009","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0021-9673(02)01114-7","article-title":"Chromatography of microbial cells using continuous supermacroporous affinity and ion-exchange columns","volume":"977","author":"Arvidsson","year":"2002","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2025.465801_bib0010","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.seppur.2008.03.026","article-title":"Macroporous elastic polyacrylamide monolith columns: processing under compression and scale-up","volume":"65","author":"Plieva","year":"2009","journal-title":"Sep. Purif. Technol."},{"key":"10.1016\/j.chroma.2025.465801_bib0011","doi-asserted-by":"crossref","first-page":"21154","DOI":"10.1038\/srep21154","article-title":"A simple method for the production of large volume 3D macroporous hydrogels for advanced biotechnological, medical and environmental applications","volume":"6","author":"Savina","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.chroma.2025.465801_bib0012","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2021.671229","article-title":"Rapid separation of human hemoglobin on a large scale from non-clarified bacterial cell homogenates using molecularly imprinted composite cryogels","volume":"9","author":"Hajizadeh","year":"2021","journal-title":"Front. Bioeng. Biotech."},{"key":"10.1016\/j.chroma.2025.465801_bib0013","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.cherd.2021.12.010","article-title":"Suspension and transformation performance of poly(2-hydroxyethyl methacrylate)-based anion exchange cryogel beads with immobilized Lactobacillus paracasei cells as biocatalysts towards biosynthesis of phenyllactic acid in stirred tank bioreactors","volume":"181","author":"Zhang","year":"2022","journal-title":"Chem. Eng. Res. Des."},{"key":"10.1016\/j.chroma.2025.465801_bib0014","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.cej.2019.03.062","article-title":"Slug flow hydrodynamics of immiscible fluids within a rectangular microchannel towards size-controllable fabrication of dextran-based cryogel beads","volume":"369","author":"Guan","year":"2019","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.chroma.2025.465801_bib0015","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.chroma.2012.05.075","article-title":"Microchannel liquid-flow focusing and cryo-polymerization preparation of supermacroporous cryogel beads for bioseparation","volume":"1247","author":"Yun","year":"2012","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2025.465801_bib0016","first-page":"2060","article-title":"Multi-microtubes formation and simulation of nanocelluloseembedded cryogel microspheres","volume":"75","author":"Wang","year":"2024","journal-title":"CIESC J."},{"key":"10.1016\/j.chroma.2025.465801_bib0017","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2022.463546","article-title":"Applications of cryostructures in the chromatographic separation of biomacromolecules","volume":"1683","author":"Babanejad","year":"2022","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2025.465801_bib0018","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodchem.2020.128295","article-title":"Strong and weak cation-exchange groups generated cryogels films for adsorption and purification of lysozyme from chicken egg white","volume":"342","author":"Bayramoglu","year":"2021","journal-title":"Food Chem."},{"key":"10.1016\/j.chroma.2025.465801_bib0019","doi-asserted-by":"crossref","DOI":"10.1016\/j.jenvman.2022.117210","article-title":"Lignin nanoparticle-decorated nanocellulose cryogels as adsorbents for pharmaceutical pollutants","volume":"330","author":"Agustin","year":"2023","journal-title":"J. Environ. Manage."},{"key":"10.1016\/j.chroma.2025.465801_bib0020","doi-asserted-by":"crossref","first-page":"3813","DOI":"10.1002\/jssc.201300911","article-title":"Poly(hydroxyethyl methacrylate)-based composite cryogel with embedded macroporous cellulose beads for the separation of human serum immunoglobulin and albumin","volume":"36","author":"Ye","year":"2013","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.chroma.2025.465801_bib0021","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.chroma.2007.05.015","article-title":"In-situ graft-polymerization preparation of cation-exchange supermacroporous cryogel with sulfo groups in glass columns","volume":"1157","author":"Yao","year":"2007","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2025.465801_bib0022","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.ces.2007.09.008","article-title":"Influence of grafting conditions on the properties of polyacrylamide-based cation-exchange cryogels grafted with 2-acrylamido-2-methyl-1-propanesulfonic acid","volume":"63","author":"Chen","year":"2008","journal-title":"Chem. Eng. Sci."},{"key":"10.1016\/j.chroma.2025.465801_bib0023","doi-asserted-by":"crossref","first-page":"2564","DOI":"10.1021\/ie504718p","article-title":"Strategy of combining prefiltration and chromatography using composite cryogels for large-scale separation of biotransformation compounds from crude high-cell-density broth","volume":"54","author":"Yun","year":"2015","journal-title":"Ind. Eng. Chem. Res."},{"key":"10.1016\/j.chroma.2025.465801_bib0024","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1016\/S1004-9541(10)60273-X","article-title":"Preparation of supermacroporous composite cryogel embedded with SiO2 nanoparticles","volume":"18","author":"Xu","year":"2010","journal-title":"Chin. J. Chem. Eng."},{"key":"10.1016\/j.chroma.2025.465801_bib0025","first-page":"5636","article-title":"Separation of phenyllactic acid from transformation broth by anion exchange poly(2-hydroxyethyl methacrylate) composite cryogel embedded with nanogels","volume":"71","author":"He","year":"2020","journal-title":"CIESC J."},{"key":"10.1016\/j.chroma.2025.465801_bib0026","doi-asserted-by":"crossref","DOI":"10.1016\/j.reactfunctpolym.2023.105693","article-title":"Application of composite cryogels in downstream processing - a review","volume":"191","author":"Hajizadeh","year":"2023","journal-title":"React. Funct. Polym."},{"key":"10.1016\/j.chroma.2025.465801_bib0027","doi-asserted-by":"crossref","first-page":"5487","DOI":"10.1016\/j.chroma.2011.06.056","article-title":"An improved capillary model for describing the microstructure characteristics, fluid hydrodynamics and breakthrough performance of proteins in cryogel beds","volume":"1218","author":"Yun","year":"2011","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2025.465801_bib0028","doi-asserted-by":"crossref","first-page":"2387","DOI":"10.1002\/jssc.201300306","article-title":"Isolation of immunoglobulin G from bovine milk whey by poly(hydroxyethyl methacrylate)-based anion-exchange cryogel","volume":"36","author":"Dong","year":"2013","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.chroma.2025.465801_bib0029","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.chroma.2018.04.043","article-title":"Chromatographic separation of phenyllactic acid from crude broth using cryogels with dual functional groups","volume":"1554","author":"Guan","year":"2018","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2025.465801_bib0030","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1016\/j.ces.2006.11.027","article-title":"Freezing characteristics of acrylamide-based aqueous solution used for the preparation of supermacroporous cryogels via cryo-copolymerization","volume":"62","author":"He","year":"2007","journal-title":"Chem. Eng. Sci."},{"key":"10.1016\/j.chroma.2025.465801_bib0031","doi-asserted-by":"crossref","first-page":"7291","DOI":"10.1021\/acs.iecr.8b00831","article-title":"Formation dynamics of cell-loading alginate droplets in the microtube dripping and cryo-cross-linking process for cell-entrapped tryogel beads as the biocatalysts toward phenyllactic acid biosynthesis","volume":"57","author":"Zhang","year":"2018","journal-title":"Ind. Eng. Chem. Res."},{"key":"10.1016\/j.chroma.2025.465801_bib0032","doi-asserted-by":"crossref","first-page":"2601","DOI":"10.1002\/jssc.200900320","article-title":"Modeling of protein breakthrough performance in cryogel columns by taking into account the overall axial dispersion","volume":"32","author":"Yun","year":"2009","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.chroma.2025.465801_bib0033","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.chroma.2006.01.014","article-title":"Characterization of a novel continuous supermacroporous monolithic cryogel embedded with nanoparticles for protein chromatography","volume":"1109","author":"Yao","year":"2006","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2025.465801_bib0034","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s002890050529","article-title":"Self-condensing vinyl polymerization of acrylamide","volume":"43","author":"Zhang","year":"1999","journal-title":"Polym. Bull."},{"key":"10.1016\/j.chroma.2025.465801_bib0035","doi-asserted-by":"crossref","first-page":"9596","DOI":"10.1016\/j.polymer.2005.07.091","article-title":"Graft polymerization of acrylic acid onto macroporous polyacrylamide gel (cryogel) initiated by potassium diperiodatocuprate","volume":"46","author":"Savina","year":"2005","journal-title":"Polymer"},{"key":"10.1016\/j.chroma.2025.465801_bib0036","doi-asserted-by":"crossref","DOI":"10.1016\/j.bej.2021.108312","article-title":"Preparation and characterization of semi-hydrophobic cryogels for culture of Lactobacillus strains and bioconversion towards phenyllactic acid bioproduction","volume":"179","author":"Lou","year":"2022","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.chroma.2025.465801_bib0037","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.chroma.2007.02.041","article-title":"Protein adsorption and transport in agarose and dextran-grafted agarose media for ion exchange chromatography","volume":"1146","author":"Stone","year":"2007","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2025.465801_bib0038","doi-asserted-by":"crossref","first-page":"2221","DOI":"10.1021\/ja02268a002","article-title":"The constitution and fundamental properties of solids and liquids","volume":"38","author":"Langmuir","year":"1916","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.chroma.2025.465801_bib0039","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.chroma.2014.11.081","article-title":"Chromatographic adsorption of serum albumin and antibody proteins in cryogels with benzyl-quaternary amine ligands","volume":"1381","author":"Yun","year":"2015","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2025.465801_bib0040","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.seppur.2015.04.026","article-title":"Separation of lactoperoxidase from bovine whey milk by cation exchange composite cryogel embedded macroporous cellulose beads","volume":"147","author":"Pan","year":"2015","journal-title":"Sep. Purif. Technol."},{"key":"10.1016\/j.chroma.2025.465801_bib0041","doi-asserted-by":"crossref","DOI":"10.1016\/j.bej.2020.107677","article-title":"Hydrophobic cryogels prepared via cryo-polymerization as oil carriers for biosynthesis of sophorolipids","volume":"161","author":"Zhang","year":"2020","journal-title":"Biochem. Eng. J."},{"key":"10.1016\/j.chroma.2025.465801_bib0042","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.chroma.2015.01.075","article-title":"Strong cation-exchange chromatography of proteins on a sulfoalkylated monolithic cryogel","volume":"1386","author":"Per\u00e7in","year":"2015","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2025.465801_bib0043","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.jhazmat.2006.11.011","article-title":"Chromium(VI) biosorption by dried rhizopus arrhizus: effect of salt (NaCl) concentration on equilibrium and kinetic parameters","volume":"145","author":"Aksu","year":"2007","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.chroma.2025.465801_bib0044","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.jhazmat.2007.12.027","article-title":"Comparison of porous poly (vinyl alcohol)\/hydroxyapatite composite cryogels and cryogels immobilized on poly (vinyl alcohol) and polyurethane foams for removal of cadmium","volume":"156","author":"Wang","year":"2008","journal-title":"J. Hazard. Mater."},{"key":"10.1016\/j.chroma.2025.465801_bib0045","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1002\/cjce.5450760419","article-title":"The kinetics of sorption of basic dyes from aqueous solution by sphagnum moss peat","volume":"76","author":"Ho","year":"1998","journal-title":"Can. J. Chem. Eng."},{"key":"10.1016\/j.chroma.2025.465801_bib0046","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/0021-9797(74)90252-5","article-title":"A general treatment and classification of the solute adsorption isotherm. I. Theoretical","volume":"47","author":"Giles","year":"1974","journal-title":"J. Colloid Interface Sci."},{"key":"10.1016\/j.chroma.2025.465801_bib0047","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2020.125354","article-title":"Freeze-thaw as a route to build manageable polysaccharide cryogel for deep cleaning of crystal violet","volume":"396","author":"Hu","year":"2020","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.chroma.2025.465801_bib0048","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.cej.2009.09.013","article-title":"Insights into the modeling of adsorption isotherm systems","volume":"156","author":"Foo","year":"2010","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.chroma.2025.465801_bib0049","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.chroma.2005.06.094","article-title":"Anion-exchange supermacroporous monolithic matrices with grafted polymer brushes of N,N-dimethylaminoethyl-methacrylate","volume":"1092","author":"Savina","year":"2005","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2025.465801_bib0050","doi-asserted-by":"crossref","first-page":"3315","DOI":"10.1002\/jssc.202000255","article-title":"Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white","volume":"43","author":"Sun","year":"2020","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.chroma.2025.465801_bib0051","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1016\/j.colsurfb.2014.10.034","article-title":"PHEMA based composite cryogels with loaded hydrophobic beads for lysozyme purification","volume":"123","author":"T\u00fcrkmen","year":"2014","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"10.1016\/j.chroma.2025.465801_bib0052","doi-asserted-by":"crossref","DOI":"10.1016\/j.carbpol.2021.118498","article-title":"Shapeable and underwater super-elastic cellulose nanofiber\/alginate cryogels by freezing-induced oxa-Michael reaction for efficient protein purification","volume":"272","author":"Tian","year":"2021","journal-title":"Carbohyd. Polym."},{"key":"10.1016\/j.chroma.2025.465801_bib0053","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2023.127494","article-title":"Preparation and characterization of lysozyme loaded cryogel for heavy metal removal","volume":"253","author":"Akkaya","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.chroma.2025.465801_bib0054","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1007\/s00289-021-03568-2","article-title":"Monolithic hydrophobic cryogel columns for protein separation","volume":"79","author":"Erzengin","year":"2022","journal-title":"Polym. Bull."},{"key":"10.1016\/j.chroma.2025.465801_bib0055","doi-asserted-by":"crossref","first-page":"4679","DOI":"10.1007\/s00289-019-02959-w","article-title":"Assessment of a new dual effective combo polymer structure for separation of lysozyme from hen egg white","volume":"77","author":"Acet","year":"2020","journal-title":"Polym. Bull."},{"key":"10.1016\/j.chroma.2025.465801_bib0056","doi-asserted-by":"crossref","first-page":"3497","DOI":"10.1002\/cjce.24703","article-title":"Performance of pseudo-specific cryogel in lysozyme purification from chicken egg white","volume":"101","author":"de O. Meira","year":"2023","journal-title":"Can. J. Chem. Eng."},{"key":"10.1016\/j.chroma.2025.465801_bib0057","doi-asserted-by":"crossref","first-page":"2012","DOI":"10.1080\/01496395.2019.1617743","article-title":"Capture of lysozyme on macroporous cryogels by hydrophobic affinity chromatography","volume":"55","author":"de Oliveira","year":"2020","journal-title":"Sep. Sci. Technol."},{"key":"10.1016\/j.chroma.2025.465801_bib0058","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.seppur.2011.08.011","article-title":"l-histidine imprinted supermacroporous cryogels for protein recognition","volume":"82","author":"Bereli","year":"2011","journal-title":"Sep. Purif. Technol."},{"key":"10.1016\/j.chroma.2025.465801_bib0059","doi-asserted-by":"crossref","first-page":"7758","DOI":"10.1002\/adma.201601066","article-title":"Highly flexible and resilient elastin hybrid cryogels with shape memory, injectability, conductivity, and magnetic responsive properties","volume":"28","author":"Liu","year":"2016","journal-title":"Adv. Mater."},{"key":"10.1016\/j.chroma.2025.465801_bib0060","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1038\/nprot.2010.135","article-title":"Cell separation using cryogel-based affinity chromatography","volume":"5","author":"Kumar","year":"2010","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.chroma.2025.465801_bib0061","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.chroma.2013.02.014","article-title":"Rapid freezing cryo-polymerization and microchannel liquid-flow focusing for cryogel beads: adsorbent preparation and characterization of supermacroporous bead-packed bed","volume":"1284","author":"Yun","year":"2013","journal-title":"J. Chromatogr. A"}],"container-title":["Journal of Chromatography A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967325001499?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967325001499?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T21:09:15Z","timestamp":1777410555000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021967325001499"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":61,"alternative-id":["S0021967325001499"],"URL":"https:\/\/doi.org\/10.1016\/j.chroma.2025.465801","relation":{},"ISSN":["0021-9673"],"issn-type":[{"value":"0021-9673","type":"print"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Mass production of cation-exchange cryogels and their chromatographic adsorption performance for bioseparation","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography A","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chroma.2025.465801","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"465801"}}