{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T08:59:25Z","timestamp":1788598765158,"version":"build-2803163510"},"reference-count":51,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,5,18]],"date-time":"2025-05-18T00:00:00Z","timestamp":1747526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1RO1GM141347\\u201301A1"],"award-info":[{"award-number":["1RO1GM141347\\u201301A1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["CHE-2107882"],"award-info":[{"award-number":["CHE-2107882"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography A"],"published-print":{"date-parts":[[2025,8]]},"DOI":"10.1016\/j.chroma.2025.466068","type":"journal-article","created":{"date-parts":[[2025,5,18]],"date-time":"2025-05-18T10:16:22Z","timestamp":1747563382000},"page":"466068","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Effects of packing density and adsorption conditions on extracellular vesicle dynamic binding capacities for capillary-channeled polymer (C-CP) fiber columns"],"prefix":"10.1016","volume":"1755","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9029-6092","authenticated-orcid":false,"given":"Md Khalid Bin","family":"Islam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4276-5805","authenticated-orcid":false,"given":"R. Kenneth","family":"Marcus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.chroma.2025.466068_bib0001","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1083\/jcb.201211138","article-title":"Extracellular vesicles: exosomes, microvesicles, and friends","volume":"200","author":"Raposo","year":"2013","journal-title":"J. Cell Biol."},{"issue":"3","key":"10.1016\/j.chroma.2025.466068_bib0002","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1038\/nri3622","article-title":"Regulation of immune responses by extracellular vesicles","volume":"14","author":"Robbins","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.chroma.2025.466068_bib0003","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.jconrel.2016.02.037","article-title":"Exosomes as therapeutics: the implications of molecular composition and exosomal heterogeneity","volume":"228","author":"Ferguson","year":"2016","journal-title":"J. Control. Release"},{"issue":"3","key":"10.1016\/j.chroma.2025.466068_bib0004","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.1038\/s41596-020-00466-1","article-title":"Isolation and characterization of extracellular vesicle subpopulations from tissues","volume":"16","author":"Crescitelli","year":"2021","journal-title":"Nat. Protoc."},{"issue":"32","key":"10.1016\/j.chroma.2025.466068_bib0005","doi-asserted-by":"crossref","first-page":"9387","DOI":"10.1021\/acs.langmuir.7b04080","article-title":"Dynamic scaling of exosome sizes","volume":"34","author":"Paulaitis","year":"2018","journal-title":"Langmuir"},{"issue":"3","key":"10.1016\/j.chroma.2025.466068_bib0006","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1074\/mcp.M116.064725","article-title":"Tracing cellular origin of human exosomes using multiplex proximity extension assays","volume":"16","author":"Larssen","year":"2017","journal-title":"Mol. Cell. Proteom."},{"key":"10.1016\/j.chroma.2025.466068_bib0007","doi-asserted-by":"crossref","DOI":"10.1016\/j.vesic.2023.100032","article-title":"Exosome as drug delivery system: current advancements","volume":"3","author":"Sharma","year":"2024","journal-title":"Extracellular Vesicle"},{"key":"10.1016\/j.chroma.2025.466068_bib0008","doi-asserted-by":"crossref","DOI":"10.1016\/j.critrevonc.2022.103628","article-title":"New advances in exosome-based targeted drug delivery systems","volume":"172","author":"Ferreira","year":"2022","journal-title":"Crit. Rev. Oncol. Hematol."},{"key":"10.1016\/j.chroma.2025.466068_bib0009","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.actbio.2020.06.036","article-title":"Exosomes as a next-generation drug delivery system: an update on drug loading approaches, characterization, and clinical application challenges","volume":"113","author":"Mehryab","year":"2020","journal-title":"Acta Biomater."},{"issue":"4","key":"10.1016\/j.chroma.2025.466068_bib0010","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1021\/acs.chemrev.7b00534","article-title":"New technologies for analysis of extracellular vesicles","volume":"118","author":"Shao","year":"2018","journal-title":"Chem. Rev."},{"issue":"11","key":"10.1016\/j.chroma.2025.466068_bib0011","doi-asserted-by":"crossref","first-page":"4739","DOI":"10.1021\/acs.analchem.1c00693","article-title":"Advances in analytical technologies for extracellular vesicles","volume":"93","author":"Yan","year":"2021","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2025.466068_bib0012","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.aca.2019.09.022","article-title":"Fast isolation of highly specific population of platelet-derived extracellular vesicles from blood plasma by affinity monolithic column, immobilized with anti-human CD61 antibody","volume":"1091","author":"Multia","year":"2019","journal-title":"Anal. Chim. Acta"},{"issue":"1","key":"10.1016\/j.chroma.2025.466068_bib0013","doi-asserted-by":"crossref","DOI":"10.1080\/20013078.2017.1294368","article-title":"Large-scale isolation and cytotoxicity of extracellular vesicles derived from activated human natural killer cells","volume":"6","author":"Jong","year":"2017","journal-title":"J. Extracell Vesicles"},{"key":"10.1016\/j.chroma.2025.466068_bib0014","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/j.jconrel.2022.05.027","article-title":"Exosomes: large-scale production, isolation, drug loading efficiency, and biodistribution and uptake","volume":"347","author":"Kimiz-Gebologlu","year":"2022","journal-title":"J. Control. Release"},{"issue":"9","key":"10.1016\/j.chroma.2025.466068_bib0015","first-page":"1390","article-title":"The updated role of exosomal proteins in the diagnosis, prognosis, and treatment of cancer","volume":"54","author":"Wang","year":"2022","journal-title":"Exp. Mol. Med."},{"issue":"4","key":"10.1016\/j.chroma.2025.466068_bib0016","doi-asserted-by":"crossref","first-page":"307","DOI":"10.3390\/cells8040307","article-title":"Review of the isolation, characterization, biological function, and multifarious therapeutic approaches of exosomes","volume":"8","author":"Gurunathan","year":"2019","journal-title":"Cells"},{"issue":"6","key":"10.1016\/j.chroma.2025.466068_bib0017","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1248\/bpb.b18-00133","article-title":"Possibility of exosome-based therapeutics and challenges in production of exosomes eligible for therapeutic application","volume":"41","author":"Yamashita","year":"2018","journal-title":"Biol. Pharmaceut. Bull."},{"issue":"2","key":"10.1016\/j.chroma.2025.466068_bib0018","doi-asserted-by":"crossref","first-page":"665","DOI":"10.3390\/ijms21020665","article-title":"Advances in analysis of biodistribution of exosomes by molecular imaging","volume":"21","author":"Yi","year":"2020","journal-title":"Int. J. Mol. Sci."},{"issue":"4","key":"10.1016\/j.chroma.2025.466068_bib0019","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1002\/elps.201800417","article-title":"Exosome isolation and purification via hydrophobic interaction chromatography using a polyester, capillary-channeled polymer fiber phase","volume":"40","author":"Bruce","year":"2019","journal-title":"Electrophoresis"},{"key":"10.1016\/j.chroma.2025.466068_bib0020","doi-asserted-by":"crossref","first-page":"4713","DOI":"10.1007\/s00216-020-02728-z","article-title":"Solid-phase extraction of exosomes from diverse matrices via a polyester capillary-channeled polymer (C-CP) fiber stationary phase in a spin-down tip format","volume":"412","author":"Jackson","year":"2020","journal-title":"Anal. Bioanal. Chem."},{"issue":"13","key":"10.1016\/j.chroma.2025.466068_bib0021","doi-asserted-by":"crossref","first-page":"3813","DOI":"10.1007\/s00216-022-04023-5","article-title":"Comparison of the capillary-channeled polymer (C-CP) fiber spin-down tip approach to traditional methods for the isolation of extracellular vesicles from human urine","volume":"414","author":"Jackson","year":"2022","journal-title":"Anal. Bioanal. Chem."},{"issue":"13","key":"10.1016\/j.chroma.2025.466068_bib0022","doi-asserted-by":"crossref","first-page":"4314","DOI":"10.1039\/D1AN00373A","article-title":"Rapid isolation of extracellular vesicles from diverse biofluid matrices via capillary-channeled polymer fiber solid-phase extraction micropipette tips","volume":"146","author":"Jackson","year":"2021","journal-title":"Analyst"},{"issue":"9","key":"10.1016\/j.chroma.2025.466068_bib0023","doi-asserted-by":"crossref","first-page":"251","DOI":"10.3390\/separations9090251","article-title":"Determination of the loading capacity and recovery of extracellular vesicles derived from human embryonic kidney cells and urine matrices on capillary-channeled polymer (C-CP) fiber columns","volume":"9","author":"Billotto","year":"2022","journal-title":"Separations"},{"key":"10.1016\/j.chroma.2025.466068_bib0024","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2021.338578","article-title":"Rapid separation of blood plasma exosomes from low-density lipoproteins via a hydrophobic interaction chromatography method on a polyester capillary-channeled polymer fiber phase","volume":"1167","author":"Huang","year":"2021","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.chroma.2025.466068_bib0025","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1007\/s00216-012-6163-8","article-title":"Microbore polypropylene capillary channeled polymer (C-CP) fiber columns for rapid reversed-phase HPLC of proteins","volume":"404","author":"Randunu","year":"2012","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.chroma.2025.466068_bib0026","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.chroma.2014.05.033","article-title":"Determination of pore size distributions in capillary-channeled polymer fiber stationary phases by inverse size-exclusion chromatography and implications for fast protein separations","volume":"1351","author":"Wang","year":"2014","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2025.466068_bib0027","doi-asserted-by":"crossref","first-page":"3811","DOI":"10.1002\/jssc.202200410","article-title":"Comparative analysis of trilobal capillary-channeled polymer Fiber columns with superficially porous and monolithic phases towards reversed-phase protein separations","volume":"45","author":"Billotto","year":"2022","journal-title":"J. Sep. Sci."},{"issue":"3","key":"10.1016\/j.chroma.2025.466068_bib0028","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.jcyt.2024.11.007","article-title":"Neural stem cell-derived extracellular vesicles purified by monolith chromatography retain stimulatory effect in in vitro scratch assay","volume":"27","author":"Colao","year":"2025","journal-title":"Cytotherapy"},{"key":"10.1016\/j.chroma.2025.466068_bib0029","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1002\/jssc.201701063","article-title":"Dynamic evaluation of a trilobal capillary-channeled polymer fiber shape for reversed phase protein separations and comparison to the eight-channeled form","author":"Wang","year":"2018","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.chroma.2025.466068_bib0030","series-title":"Fundamentals of Preparative and Nonlinear Chromatography","author":"Guiochon","year":"2006"},{"key":"10.1016\/j.chroma.2025.466068_bib0031","series-title":"Dynamics of chromatography: Principles and Theory","author":"Giddings","year":"2017"},{"issue":"7","key":"10.1016\/j.chroma.2025.466068_bib0032","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12093","article-title":"Urinary extracellular vesicles: a position paper by the urine task force of the international society for extracellular vesicles","volume":"10","author":"Erdbr\u00fcgger","year":"2021","journal-title":"J. Extracell Vesicles"},{"key":"10.1016\/j.chroma.2025.466068_bib0033","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1007\/s00216-005-0117-3","article-title":"Capillary-channeled polymer (C-CP) fibers as a stationary phase in microbore high-performance liquid chromatography columns","volume":"384","author":"Stanelle","year":"2006","journal-title":"Anal. Bioanal. Chem."},{"issue":"1","key":"10.1016\/j.chroma.2025.466068_bib0034","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.chroma.2005.09.014","article-title":"Hydrodynamic flow in capillary-channel fiber columns for liquid chromatography","volume":"1100","author":"Stanelle","year":"2005","journal-title":"J. Chromatogr. A"},{"issue":"1","key":"10.1016\/j.chroma.2025.466068_bib0035","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1002\/btpr.2013","article-title":"Roles of interstitial fraction and load conditions on the dynamic binding capacity of proteins on capillary-channeled polymer fiber columns","volume":"31","author":"Wang","year":"2015","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.chroma.2025.466068_bib0036","series-title":"Protein Chromatography: Process Development and Scale-Up","author":"Carta","year":"2020"},{"key":"10.1016\/j.chroma.2025.466068_bib0037","series-title":"Dynamics of Chromatography","author":"Giddings","year":"1965"},{"key":"10.1016\/j.chroma.2025.466068_bib0038","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.chroma.2011.12.081","article-title":"Highly crosslinked polymeric monoliths for reversed-phase capillary liquid chromatography of small molecules","volume":"1227","author":"Liu","year":"2012","journal-title":"J. Chromatogr. A"},{"issue":"6","key":"10.1016\/j.chroma.2025.466068_bib0039","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1039\/B816712E","article-title":"A novel glycidyl methacrylate-based monolith with sub-micron skeletons and well-defined macropores","volume":"19","author":"Yao","year":"2009","journal-title":"J. Mater. Chem."},{"issue":"14","key":"10.1016\/j.chroma.2025.466068_bib0040","doi-asserted-by":"crossref","DOI":"10.1002\/marc.202170052","article-title":"One-pot fabrication of hierarchically bicontinuous polystyrene monoliths with homogeneous skeletons and glycopolymer surfaces","volume":"42","author":"Peng","year":"2021","journal-title":"Macromol. Rapid Commun."},{"key":"10.1016\/j.chroma.2025.466068_bib0041","series-title":"Unified Separation Science","author":"Giddings","year":"1991"},{"issue":"23","key":"10.1016\/j.chroma.2025.466068_bib0042","doi-asserted-by":"crossref","first-page":"3270","DOI":"10.1002\/jssc.201200523","article-title":"Dynamic evaluation of polypropylene capillary-channeled fibers as a stationary phase in high-performance liquid chromatography","volume":"35","author":"Randunu","year":"2012","journal-title":"J. Sep. Sci."},{"issue":"5","key":"10.1016\/j.chroma.2025.466068_bib0043","doi-asserted-by":"crossref","first-page":"e2998","DOI":"10.1002\/btpr.2998","article-title":"Evaluation of exosome loading characteristics in their purification via a glycerol-assisted hydrophobic interaction chromatography method on a polyester, capillary-channeled polymer fiber phase","volume":"36","author":"Huang","year":"2020","journal-title":"Biotechnol. Prog."},{"issue":"1","key":"10.1016\/j.chroma.2025.466068_bib0044","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1515\/chem-2022-0139","article-title":"Comparison of the yield and purity of plasma exosomes extracted by ultracentrifugation, precipitation, and membrane-based approaches","volume":"20","author":"Li","year":"2022","journal-title":"Open Chem."},{"key":"10.1016\/j.chroma.2025.466068_bib0045","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jviromet.2018.10.004","article-title":"Comparison of exosomes purified via ultracentrifugation (UC) and Total Exosome Isolation (TEI) reagent from the serum of Marek\u2019s disease virus (MDV)-vaccinated and tumor-bearing chickens","volume":"263","author":"Neerukonda","year":"2019","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.chroma.2025.466068_bib0046","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.chroma.2018.11.068","article-title":"Evaluation of protein separations based on hydrophobic interaction chromatography using polyethylene terephthalate capillary-channeled polymer (C-CP) fiber phases","volume":"1585","author":"Wang","year":"2019","journal-title":"J. Chromatogr. A"},{"issue":"5","key":"10.1016\/j.chroma.2025.466068_bib0047","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.ccell.2014.09.005","article-title":"Cancer exosomes perform cell-independent microRNA biogenesis and promote tumorigenesis","volume":"26","author":"Melo","year":"2014","journal-title":"Cancer Cell"},{"issue":"1","key":"10.1016\/j.chroma.2025.466068_bib0048","article-title":"The complete exosome workflow solution: from isolation to characterization of RNA cargo","volume":"2013","author":"Schageman","year":"2013","journal-title":"Biomed. Res. Int."},{"issue":"1","key":"10.1016\/j.chroma.2025.466068_bib0049","doi-asserted-by":"crossref","DOI":"10.3402\/jev.v2i0.22390","article-title":"Cancer becomes wasteful: emerging roles of exosomes\u2020 in cell-fate determination","volume":"2","author":"Wendler","year":"2013","journal-title":"J. Extracell Vesicles"},{"issue":"1","key":"10.1016\/j.chroma.2025.466068_bib0050","doi-asserted-by":"crossref","DOI":"10.3402\/jev.v2i0.20304","article-title":"Emerging roles of extracellular vesicles in the adaptive response of tumour cells to microenvironmental stress","volume":"2","author":"Kucharzewska","year":"2013","journal-title":"J. Extracell Vesicles"},{"key":"10.1016\/j.chroma.2025.466068_bib0051","series-title":"Bioseparations Engineering: Principles, Practice, and Economics","author":"Ladisch","year":"2001"}],"container-title":["Journal of Chromatography A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967325004157?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967325004157?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T02:06:25Z","timestamp":1778637985000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021967325004157"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":51,"alternative-id":["S0021967325004157"],"URL":"https:\/\/doi.org\/10.1016\/j.chroma.2025.466068","relation":{},"ISSN":["0021-9673"],"issn-type":[{"value":"0021-9673","type":"print"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Effects of packing density and adsorption conditions on extracellular vesicle dynamic binding capacities for capillary-channeled polymer (C-CP) fiber columns","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography A","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chroma.2025.466068","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"466068"}}