{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T21:19:12Z","timestamp":1787606352410,"version":"build-2736575974"},"reference-count":28,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography A"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.chroma.2026.467306","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T23:56:58Z","timestamp":1785283018000},"page":"467306","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Considerations on column length in gradient elution mode: \u201cselectivity-limited\u201d and \u201cefficiency limited\u201d separations"],"prefix":"10.1016","volume":"1785","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7357-0691","authenticated-orcid":false,"given":"Szabolcs","family":"Fekete","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"35","key":"10.1016\/j.chroma.2026.467306_bib0001","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1042\/bj0351358","article-title":"A new form of chromatogram employing two liquid phases","author":"Martin","year":"1941","journal-title":"Biochem. J."},{"key":"10.1016\/j.chroma.2026.467306_bib0002","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0009-2509(56)80003-1","article-title":"Longitudinal diffusion and resistance to mass transfer as causes of nonideality in chromatography","volume":"5","author":"van Deemter","year":"1956","journal-title":"Chem. Eng. Sci."},{"key":"10.1016\/j.chroma.2026.467306_bib0003","series-title":"Dynamics of chromatography, Part 1, Principles and theory","author":"Giddings","year":"1965"},{"key":"10.1016\/j.chroma.2026.467306_bib0004","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1093\/chromsci\/7.2.109","article-title":"Column design in high pressure liquid chromatography","volume":"7","author":"Horvath","year":"1969","journal-title":"J. Chrom. Sci."},{"key":"10.1016\/j.chroma.2026.467306_bib0005","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1002\/anie.198200501","article-title":"Optimal parameters in high-speed liquid chromatography (HPLC)","volume":"21","author":"Hal\u00e1sz","year":"1982","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.chroma.2026.467306_bib0006","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1093\/chromsci\/23.3.106","article-title":"Peak variance as a function of HPLC column length and diameter","volume":"23","author":"Rocca","year":"1985","journal-title":"J. Chrom. Sci."},{"key":"10.1016\/j.chroma.2026.467306_bib0007","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0021-9673(00)82241-4","article-title":"Construction of columns for liquid chromatography with very large plate numbers: Theory and practice","volume":"122","author":"Kraak","year":"1976","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467306_bib0008","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.chroma.2005.03.008","article-title":"Theory of peak capacity in gradient elution","volume":"1079","author":"Neue","year":"2005","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467306_bib0009","doi-asserted-by":"crossref","first-page":"3017","DOI":"10.1021\/ac3033307","article-title":"Perspectives on the evolution of the column efficiency in liquid chromatography","volume":"85","author":"Gritti","year":"2013","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2026.467306_bib0010","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.chroma.2005.12.050","article-title":"A practical approach to maximizing peak capacity by using long columns packed with pellicular stationary phases for proteomic research","volume":"1107","author":"Wang","year":"2006","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467306_bib0011","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0021-9673(97)01045-5","article-title":"Maintaining fixed band spacing when changing column dimensions in gradient elution","volume":"799","author":"Dolan","year":"1988","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467306_bib0012","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S0021-9673(01)87944-9","article-title":"Larger-diameter small-particle columns for fast high-performance liquid chromatographic separations with conventional equipment","volume":"261","author":"Stout","year":"1983","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467306_bib0013","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.chroma.2007.02.055","article-title":"Method to predict and compare the influence of the particle size on the isocratic peak capacity of high-performance liquid chromatography columns","volume":"1147","author":"Cabooter","year":"2007","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467306_bib0014","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.chroma.2015.07.014","article-title":"Comparison of the most recent chromatographic approaches applied for fast and high resolution separations: Theory and practice","volume":"1408","author":"Fekete","year":"2015","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467306_bib0015","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/0021-9673(94)01254-C","article-title":"High-speed high-performance liquid chromatography of peptides and proteins","volume":"705","author":"Chen","year":"1995","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467306_bib0016","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0021-9673(97)00376-2","article-title":"Some reflections on speed and efficiency of modern chromatographic methods","volume":"778","author":"Poppe","year":"1997","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467306_bib0017","doi-asserted-by":"crossref","first-page":"4058","DOI":"10.1021\/ac050160z","article-title":"Geometry-independent plate height representation methods for the direct comparison of the kinetic performance of lc supports with a different size or morphology","volume":"77","author":"Desmet","year":"2005","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2026.467306_bib0018","doi-asserted-by":"crossref","first-page":"2150","DOI":"10.1021\/ac051280+","article-title":"Practical constraints in the kinetic plot representation of chromatographic performance data: Theory and application to experimental data","volume":"78","author":"Desmet","year":"2006","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2026.467306_bib0019","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.chroma.2008.09.106","article-title":"Kinetic plot method as a tool to design coupled column systems producing 100,000 theoretical plates in the shortest possible time","volume":"1212","author":"Cabooter","year":"2008","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467306_bib0020","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.chroma.2008.07.007","article-title":"Kinetic plot and particle size distribution analysis to discuss the performance limits of sub-2 \u03bcm and supra-2 \u03bcm particle columns","volume":"1204","author":"Cabooter","year":"2008","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467306_bib0021","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.chroma.2013.08.008","article-title":"Kinetic evaluation of new generation of column packed with 1.3 \u03bcm core\u2013shell particles","volume":"1308","author":"Fekete","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467306_bib0022","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.jpba.2017.04.004","article-title":"Achievable separation performance and analysis time in current liquid chromatographic practice for monoclonal antibody separations","volume":"141","author":"Fekete","year":"2017","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.chroma.2026.467306_bib0023","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.chroma.2006.12.101","article-title":"Use of the kinetic plot method to analyze commercial high-temperature liquid chromatography systems: I: Intrinsic performance comparison","volume":"1143","author":"Cabooter","year":"2007","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467306_bib0024","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.ejpb.2006.11.027","article-title":"Method transfer for fast liquid chromatography in pharmaceutical analysis: Application to short columns packed with small particle. Part I: Isocratic separation","volume":"66","author":"Guillarme","year":"2007","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"10.1016\/j.chroma.2026.467306_bib0025","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.ejpb.2007.06.018","article-title":"Method transfer for fast liquid chromatography in pharmaceutical analysis: Application to short columns packed with small particle. Part II: Gradient experiments","volume":"68","author":"Guillarme","year":"2008","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"10.1016\/j.chroma.2026.467306_bib0026","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1039\/jr9600001268","article-title":"The correlation of separating power and efficiency of gas-chromatographic columns","author":"Purnell","year":"1960","journal-title":"J. Chem. Soc."},{"key":"10.1016\/j.chroma.2026.467306_bib0027","series-title":"High-performance gradient elution: The practical application of the linear-solvent-strength model","author":"Snyder","year":"2007"},{"key":"10.1016\/j.chroma.2026.467306_bib0028","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0021-9673(00)85726-X","article-title":"Gradient elution in high-performance liquid chromatography: I. Theoretical basis for reversed-phase systems","volume":"165","author":"Snyder","year":"1979","journal-title":"J. Chromatogr. A"}],"container-title":["Journal of Chromatography A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967326006345?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967326006345?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T20:23:30Z","timestamp":1787603010000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021967326006345"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":28,"alternative-id":["S0021967326006345"],"URL":"https:\/\/doi.org\/10.1016\/j.chroma.2026.467306","relation":{},"ISSN":["0021-9673"],"issn-type":[{"value":"0021-9673","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Considerations on column length in gradient elution mode: \u201cselectivity-limited\u201d and \u201cefficiency limited\u201d separations","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography A","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chroma.2026.467306","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"467306"}}