{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T12:58:58Z","timestamp":1790427538957,"version":"4.1.0"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P41-GM108538"],"award-info":[{"award-number":["P41-GM108538"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography A"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.chroma.2026.467400","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T23:12:35Z","timestamp":1787958755000},"page":"467400","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Comparison of ultrahigh- and low-pressure packing methods for preparation of 50 cm long capillary UHPLC columns"],"prefix":"10.1016","volume":"1787","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-4791-2781","authenticated-orcid":false,"given":"Tate A.","family":"Hancock","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2447-7471","authenticated-orcid":false,"given":"Robert T.","family":"Kennedy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.chroma.2026.467400_bib0001","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.chroma.2017.05.039","article-title":"Recent advances in capillary ultrahigh pressure liquid chromatography","volume":"1523","author":"Blue","year":"2017","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467400_bib0002","unstructured":"F. Gritti, M.F. Wahab, Understanding the science behind packing high-efficiency columns and capillaries: facts, fundamentals, challenges, and future directions, 36 (2018) 82\u201398."},{"issue":"16","key":"10.1016\/j.chroma.2026.467400_bib0003","doi-asserted-by":"crossref","first-page":"8177","DOI":"10.1021\/acs.analchem.7b00931","article-title":"Fundamental and practical insights on the packing of modern high-efficiency analytical and capillary columns","volume":"89","author":"Wahab","year":"2017","journal-title":"Anal. Chem."},{"issue":"4","key":"10.1016\/j.chroma.2026.467400_bib0004","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.cels.2016.10.007","article-title":"Now, more than ever, proteomics needs better chromatography","volume":"3","author":"Shishkova","year":"2016","journal-title":"Cell. Syst."},{"key":"10.1016\/j.chroma.2026.467400_bib0005","article-title":"State-of-the-art in capillary liquid chromatography (LC): now","volume":"38","author":"Grinias","year":"2020","journal-title":"Next How?"},{"issue":"19","key":"10.1016\/j.chroma.2026.467400_bib0006","doi-asserted-by":"crossref","first-page":"11503","DOI":"10.1021\/acs.analchem.8b02766","article-title":"Ultra-high pressure (>30,000 psi) packing of capillary columns enhancing depth of shotgun proteomic analyses","volume":"90","author":"Shishkova","year":"2018","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2026.467400_bib0007","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2020.461706","article-title":"Capillary ultrahigh-pressure liquid chromatography-mass spectrometry for fast and high resolution metabolomics separations","volume":"1635","author":"Sorensen","year":"2021","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467400_bib0008","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2019.460575","article-title":"Ultrahigh-performance capillary liquid chromatography-mass spectrometry at 35 kpsi for separation of lipids","volume":"1611","author":"Sorensen","year":"2020","journal-title":"J. Chromatogr. A"},{"issue":"22","key":"10.1016\/j.chroma.2026.467400_bib0009","doi-asserted-by":"crossref","first-page":"4094","DOI":"10.1002\/jssc.202000545","article-title":"Comparison of microcapillary column length and inner diameter investigated with gradient analysis of lipids by ultrahigh-pressure liquid chromatography-mass spectrometry","volume":"43","author":"Miller","year":"2020","journal-title":"J. Sep. Sci."},{"issue":"16","key":"10.1016\/j.chroma.2026.467400_bib0010","doi-asserted-by":"crossref","first-page":"4235","DOI":"10.1021\/ac0202280","article-title":"High-efficiency nanoscale liquid chromatography coupled on-line with mass spectrometry using nanoelectrospray ionization for proteomics","volume":"74","author":"Shen","year":"2002","journal-title":"Anal. Chem."},{"issue":"10","key":"10.1016\/j.chroma.2026.467400_bib0011","doi-asserted-by":"crossref","first-page":"3090","DOI":"10.1021\/ac0483062","article-title":"Automated 20 kpsi RPLC-MS and MS\/MS with chromatographic peak capacities of 1000\u22121500 and capabilities in proteomics and metabolomics","volume":"77","author":"Shen","year":"2005","journal-title":"Anal. Chem."},{"issue":"10","key":"10.1016\/j.chroma.2026.467400_bib0012","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.1021\/ac00185a016","article-title":"Preparation and evaluation of packed capillary liquid chromatography columns with inner diameters from 20 to 50 micrometers","volume":"61","author":"Kennedy","year":"1989","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2026.467400_bib0013","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.chroma.2014.12.055","article-title":"1.1\u03bcm Superficially porous particles for liquid chromatography: part II: column packing and chromatographic performance","volume":"1380","author":"Blue","year":"2015","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467400_bib0014","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.chroma.2013.10.017","article-title":"Slurry concentration effects on the bed morphology and separation efficiency of capillaries packed with sub-2\u03bcm particles","volume":"1318","author":"Bruns","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467400_bib0015","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.chroma.2018.07.037","article-title":"Axial heterogeneities in capillary ultrahigh pressure liquid chromatography columns: chromatographic and bed morphological characterization","volume":"1569","author":"Reising","year":"2018","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467400_bib0016","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.chroma.2017.05.007","article-title":"Bed morphological features associated with an optimal slurry concentration for reproducible preparation of efficient capillary ultrahigh pressure liquid chromatography columns","volume":"1504","author":"Reising","year":"2017","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467400_bib0017","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.chroma.2016.08.002","article-title":"Implementation of high slurry concentration and sonication to pack high-efficiency, meter-long capillary ultrahigh pressure liquid chromatography columns","volume":"1462","author":"Godinho","year":"2016","journal-title":"J. Chromatogr. A"},{"issue":"15\u201317","key":"10.1016\/j.chroma.2026.467400_bib0018","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1002\/1615-9314(20021101)25:15\/17<1215::AID-JSSC1215>3.0.CO;2-O","article-title":"Comparison of column packing techniques for capillary electrochromatography","volume":"25","author":"Maloney","year":"2002","journal-title":"J. Sep. Sci."},{"key":"10.1016\/j.chroma.2026.467400_bib0019","series-title":"Capillary Column Technologies: Efficiencies, Throughput, Reproducibility, and What\u2019s Next? |","author":"Sun","year":"2026"},{"key":"10.1016\/j.chroma.2026.467400_bib0020","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.chroma.2012.10.027","article-title":"Influence of particle properties on the wall region in packed capillaries","volume":"1268","author":"Bruns","year":"2012","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467400_bib0021","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.chroma.2016.01.068","article-title":"Larger voids in mechanically stable, loose packings of 1.3\u03bcm frictional, cohesive particles: their reconstruction, statistical analysis, and impact on separation efficiency","volume":"1436","author":"Reising","year":"2016","journal-title":"J. Chromatogr. A"},{"issue":"10","key":"10.1016\/j.chroma.2026.467400_bib0022","doi-asserted-by":"crossref","first-page":"4496","DOI":"10.1021\/ac300326k","article-title":"Morphology and separation efficiency of low-aspect-ratio capillary ultrahigh pressure liquid chromatography columns","volume":"84","author":"Bruns","year":"2012","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2026.467400_bib0023","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2024.464856","article-title":"Preparation of high-efficiency HILIC capillary columns utilizing slurry packing at 2100 bar","volume":"1722","author":"Anderson","year":"2024","journal-title":"J. Chromatogr. A"},{"issue":"9","key":"10.1016\/j.chroma.2026.467400_bib0024","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1002\/(SICI)1520-667X(199911)11:9<631::AID-MCS1>3.0.CO;2-I","article-title":"Fast ultrahigh-pressure liquid chromatography: on-column UV and time-of-flight mass spectrometric detection","volume":"11","author":"Lippert","year":"1999","journal-title":"J. Microcolumn Sep."},{"issue":"6","key":"10.1016\/j.chroma.2026.467400_bib0025","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1021\/ac961094r","article-title":"Ultrahigh-pressure reversed-phase liquid chromatography in packed capillary columns","volume":"69","author":"MacNair","year":"1997","journal-title":"Anal. Chem."},{"issue":"1","key":"10.1016\/j.chroma.2026.467400_bib0026","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0021-9673(00)01188-2","article-title":"Practical aspects of ultrahigh pressure capillary liquid chromatography","volume":"911","author":"Wu","year":"2001","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467400_bib0027","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcpro.2021.100082","article-title":"A new parallel high-pressure packing system enables rapid multiplexed production of capillary columns","volume":"20","author":"M\u00fcller-Reif","year":"2021","journal-title":"Mol. Cell. Proteom."},{"issue":"3","key":"10.1016\/j.chroma.2026.467400_bib0028","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1002\/mcs.1220070311","article-title":"Theoretical studies of the preparation of packed capillary columns for chromatography","volume":"7","author":"Tong","year":"1995","journal-title":"J. Microcolumn Sep."},{"issue":"2","key":"10.1016\/j.chroma.2026.467400_bib0029","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1002\/(SICI)1520-667X(1997)9:2<97::AID-MCS6>3.0.CO;2-0","article-title":"Factors affecting the preparation of packed capillary columns in supercritical carbon dioxide media","volume":"9","author":"Koivisto","year":"1997","journal-title":"J. Microcolumn Sep."},{"issue":"2","key":"10.1016\/j.chroma.2026.467400_bib0030","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1074\/mcp.TIR118.000953","article-title":"FlashPack: fast and simple preparation of ultrahigh-performance capillary columns for LC-MS*[S]","volume":"18","author":"Kovalchuk","year":"2019","journal-title":"Mol. Cell. Proteom."},{"key":"10.1016\/j.chroma.2026.467400_bib0031","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.chroma.2018.07.011","article-title":"Characterization of radial and axial heterogeneities of chromatographic columns by flow reversal","volume":"1567","author":"Gritti","year":"2018","journal-title":"J. Chromatogr. A"},{"issue":"15","key":"10.1016\/j.chroma.2026.467400_bib0032","doi-asserted-by":"crossref","first-page":"3847","DOI":"10.1002\/pmic.200402010","article-title":"Self-made frits for nanoscale columns in proteomics","volume":"5","author":"Maiolica","year":"2005","journal-title":"PROTEOMICS"},{"key":"10.1016\/j.chroma.2026.467400_bib0033","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.chroma.2015.10.013","article-title":"Highly efficient capillary columns packed with superficially porous particles via sequential column packing","volume":"1422","author":"Treadway","year":"2015","journal-title":"J. Chromatogr. A"},{"issue":"3","key":"10.1016\/j.chroma.2026.467400_bib0034","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1021\/ac9807013","article-title":"Ultrahigh-pressure reversed-phase capillary liquid chromatography:\u2009 isocratic and gradient elution using columns packed with 1.0-\u03bcm particles","volume":"71","author":"MacNair","year":"1999","journal-title":"Anal. Chem."},{"issue":"19","key":"10.1016\/j.chroma.2026.467400_bib0035","doi-asserted-by":"crossref","first-page":"5777","DOI":"10.1021\/ac049756x","article-title":"In-depth characterization of slurry packed capillary columns with 1.0-\u03bcm nonporous particles using reversed-phase isocratic ultrahigh-pressure liquid chromatography","volume":"76","author":"Patel","year":"2004","journal-title":"Anal. Chem."},{"issue":"7","key":"10.1016\/j.chroma.2026.467400_bib0036","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1021\/ac950682m","article-title":"Preparation and evaluation of slurry-packed liquid chromatography microcolumns with inner diameters from 12 to 33 \u03bcm","volume":"68","author":"Hsieh","year":"1996","journal-title":"Anal. Chem."},{"issue":"8","key":"10.1016\/j.chroma.2026.467400_bib0037","doi-asserted-by":"crossref","first-page":"2860","DOI":"10.1021\/ac802467k","article-title":"Capillary-based instrument for the simultaneous measurement of solution viscosity and solute diffusion coefficient at pressures up to 2000 bar and implications for ultrahigh pressure liquid chromatography","volume":"81","author":"Kaiser","year":"2009","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2026.467400_bib0038","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.chroma.2016.09.053","article-title":"Development of a 45 kpsi ultrahigh pressure liquid chromatography instrument for gradient separations of peptides using long microcapillary columns and sub-2 \u00b5m particles","volume":"1469","author":"Grinias","year":"2016","journal-title":"J. Chromatogr. A"},{"issue":"18","key":"10.1016\/j.chroma.2026.467400_bib0039","doi-asserted-by":"crossref","first-page":"5441","DOI":"10.1021\/ac049643d","article-title":"Use of 1.5-\u03bcm porous ethyl-bridged hybrid particles as a stationary-phase support for reversed-phase ultrahigh-pressure liquid chromatography","volume":"76","author":"Mellors","year":"2004","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2026.467400_bib0040","series-title":"Utilization of Long Columns Packed with Sub-2 \u03bcm Particles Operated at High Pressures and Elevated Temperatures For High-Efficiency One-Dimensional Liquid Chromatographic Separations","author":"Franklin","year":"2012"},{"issue":"1","key":"10.1016\/j.chroma.2026.467400_bib0041","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.compchemeng.2008.09.003","article-title":"Effect of particle size distribution and packing compression on fluid permeability as predicted by lattice-boltzmann simulations","volume":"33","author":"Vidal","year":"2009","journal-title":"Comput. Chem. Eng."},{"issue":"6","key":"10.1016\/j.chroma.2026.467400_bib0042","doi-asserted-by":"crossref","first-page":"3017","DOI":"10.1021\/ac3033307","article-title":"Perspectives on the evolution of the column efficiency in liquid chromatography","volume":"85","author":"Gritti","year":"2013","journal-title":"Anal. Chem."},{"issue":"16","key":"10.1016\/j.chroma.2026.467400_bib0043","doi-asserted-by":"crossref","first-page":"7057","DOI":"10.1021\/ac901187d","article-title":"Time and length scales of eddy dispersion in chromatographic beds","volume":"81","author":"Khirevich","year":"2009","journal-title":"Anal. Chem."},{"issue":"1","key":"10.1016\/j.chroma.2026.467400_bib0044","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1146\/annurev.anchem.1.031207.113014","article-title":"Capillary liquid chromatography at ultrahigh pressures","volume":"3","author":"Jorgenson","year":"2010","journal-title":"Annu. Rev. Anal. Chem."}],"container-title":["Journal of Chromatography A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967326007260?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967326007260?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T12:18:38Z","timestamp":1790425118000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021967326007260"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":44,"alternative-id":["S0021967326007260"],"URL":"https:\/\/doi.org\/10.1016\/j.chroma.2026.467400","relation":{},"ISSN":["0021-9673"],"issn-type":[{"value":"0021-9673","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Comparison of ultrahigh- and low-pressure packing methods for preparation of 50 cm long capillary UHPLC columns","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography A","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chroma.2026.467400","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"467400"}}