{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T12:53:12Z","timestamp":1791636792903,"version":"4.3.4"},"reference-count":77,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100013804","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013804","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21974045"],"award-info":[{"award-number":["21974045"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Chromatography A"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.chroma.2026.467505","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T15:15:26Z","timestamp":1790608526000},"page":"467505","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Visualized separation of exosomes in urine using carrier ampholyte-free free-flow isoelectric focusing electrophoresis"],"prefix":"10.1016","volume":"1788","author":[{"given":"Haoran","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junfeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfeng","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weibing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.chroma.2026.467505_bib0001","article-title":"The biology, function, and biomedical applications of exosomes","volume":"367","author":"Kalluri","year":"2020","journal-title":"Science (1979)"},{"key":"10.1016\/j.chroma.2026.467505_bib0002","doi-asserted-by":"crossref","first-page":"2939","DOI":"10.1016\/j.ymthe.2024.02.025","article-title":"Therapeutic potential of RNA-enriched extracellular vesicles: the next generation in RNA delivery via biogenic nanoparticles","volume":"32","author":"Muskan","year":"2024","journal-title":"Mol. Ther."},{"key":"10.1016\/j.chroma.2026.467505_bib0003","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/s41556-018-0250-9","article-title":"Specificities of secretion and uptake of exosomes and other extracellular vesicles for cell-to-cell communication","volume":"21","author":"Mathieu","year":"2019","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.chroma.2026.467505_bib0004","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1038\/nrd3978","article-title":"Extracellular vesicles: biology and emerging therapeutic opportunities","volume":"12","author":"El Andaloussi","year":"2013","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.chroma.2026.467505_bib0005","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/s00018-017-2595-9","article-title":"Current knowledge on exosome biogenesis and release","volume":"75","author":"Hessvik","year":"2018","journal-title":"Cell Mol. Life Sci."},{"key":"10.1016\/j.chroma.2026.467505_bib0006","doi-asserted-by":"crossref","DOI":"10.1016\/j.exer.2021.108892","article-title":"Exosomes, extracellular vesicles and the eye","volume":"214","author":"Rudraprasad","year":"2022","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.chroma.2026.467505_bib0007","doi-asserted-by":"crossref","first-page":"13368","DOI":"10.1073\/pnas.0403453101","article-title":"Identification and proteomic profiling of exosomes in human urine","volume":"101","author":"Pisitkun","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.chroma.2026.467505_bib0008","doi-asserted-by":"crossref","first-page":"7134","DOI":"10.3390\/ijms23137134","article-title":"Isolation and characterization of urinary extracellular vesicles from healthy donors and patients with castration-resistant prostate cancer","volume":"23","author":"Lee","year":"2022","journal-title":"IJMS"},{"key":"10.1016\/j.chroma.2026.467505_bib0009","doi-asserted-by":"crossref","DOI":"10.1002\/smsc.202100118","article-title":"Investigation of urinary exosome metabolic patterns in membranous nephropathy by titania-assisted intact exosome mass spectrometry","volume":"2","author":"Chen","year":"2022","journal-title":"Small Sci."},{"key":"10.1016\/j.chroma.2026.467505_bib0010","doi-asserted-by":"crossref","first-page":"3308","DOI":"10.1021\/acs.jproteome.8b00459","article-title":"Highly efficient phosphoproteome capture and analysis from urinary extracellular vesicles","volume":"17","author":"Wu","year":"2018","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.chroma.2026.467505_bib0011","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1186\/s12951-025-03768-2","article-title":"Advances of extracellular vesicles isolation and detection frontier technology: from heterogeneity analysis to clinical application","volume":"23","author":"Li","year":"2025","journal-title":"J. Nanobiotechnol."},{"key":"10.1016\/j.chroma.2026.467505_bib0012","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1038\/s41556-018-0040-4","article-title":"Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation","volume":"20","author":"Zhang","year":"2018","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.chroma.2026.467505_bib0013","doi-asserted-by":"crossref","first-page":"4237","DOI":"10.1039\/D2AN01268E","article-title":"Efficient exosome subpopulation isolation and proteomic profiling using a sub-ExoProfile chip towards cancer diagnosis and treatment","volume":"147","author":"Wang","year":"2022","journal-title":"Analyst"},{"key":"10.1016\/j.chroma.2026.467505_bib0014","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12404","article-title":"Minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches","volume":"13","author":"Welsh","year":"2024","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.chroma.2026.467505_bib0015","doi-asserted-by":"crossref","DOI":"10.1016\/j.tranon.2024.102121","article-title":"A comprehensive review on recent advances in exosome isolation and characterization: toward clinical applications","volume":"50","author":"Dilsiz","year":"2024","journal-title":"Transl. Oncol."},{"key":"10.1016\/j.chroma.2026.467505_bib0016","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.procbio.2019.09.017","article-title":"Development and comparative analysis of human urine exosome isolation strategies","volume":"88","author":"Cho","year":"2020","journal-title":"Process. Biochem."},{"key":"10.1016\/j.chroma.2026.467505_bib0017","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2020.461773","article-title":"Modern isolation and separation techniques for extracellular vesicles","volume":"1636","author":"Liangsupree","year":"2021","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467505_bib0018","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1139\/o92-028","article-title":"Maturation of reticulocytes: formation of exosomes as a mechanism for shedding membrane proteins","volume":"70","author":"Johnstone","year":"1992","journal-title":"Biochem. Cell Biol."},{"key":"10.1016\/j.chroma.2026.467505_bib0019","doi-asserted-by":"crossref","DOI":"10.3402\/jev.v3.25011","article-title":"Comparative analysis of discrete exosome fractions obtained by differential centrifugation","volume":"3","author":"Jeppesen","year":"2014","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.chroma.2026.467505_bib0020","doi-asserted-by":"crossref","DOI":"10.1080\/20013078.2020.1791450","article-title":"A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity","volume":"9","author":"Zhang","year":"2020","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.chroma.2026.467505_bib0021","doi-asserted-by":"crossref","DOI":"10.1002\/jex2.167","article-title":"Optimization of ultracentrifugation-based method to enhance the purity and proteomic profiling depth of plasma-derived extracellular vesicles and particles","volume":"3","author":"Wan","year":"2024","journal-title":"J. Extracell. Biol."},{"key":"10.1016\/j.chroma.2026.467505_bib0022","article-title":"Isolation of high-purity extracellular vesicles by extracting proteins using aqueous two-phase system","volume":"10","author":"Kim","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.chroma.2026.467505_bib0023","doi-asserted-by":"crossref","DOI":"10.1038\/srep23978","article-title":"ExtraPEG: a polyethylene glycol-based method for enrichment of extracellular vesicles","volume":"6","author":"Rider","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.chroma.2026.467505_bib0024","doi-asserted-by":"crossref","DOI":"10.1002\/biot.202400374","article-title":"Optimizing conditions of polyethylene glycol precipitation for exosomes isolation from MSCs culture media for regenerative treatment","volume":"19","author":"Yu","year":"2024","journal-title":"Biotechnol. J."},{"key":"10.1016\/j.chroma.2026.467505_bib0025","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1186\/s12964-025-02207-x","article-title":"Comparative evaluation of methods for isolating extracellular vesicles from ICC cell culture supernatants: insights into proteomic and glycomic analysis","volume":"23","author":"Wu","year":"2025","journal-title":"Cell Commun. Signal."},{"key":"10.1016\/j.chroma.2026.467505_bib0026","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12145","article-title":"Establishment of a simplified dichotomic size-exclusion chromatography for isolating extracellular vesicles toward clinical applications","volume":"10","author":"Guo","year":"2021","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.chroma.2026.467505_bib0027","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.3390\/cells10061328","article-title":"Exosome-based molecular transfer activity of macrophage-like cells involves viability of oral carcinoma cells: size exclusion chromatography and concentration filter method","volume":"10","author":"Lu","year":"2021","journal-title":"Cells"},{"key":"10.1016\/j.chroma.2026.467505_bib0028","doi-asserted-by":"crossref","DOI":"10.1002\/pmic.202200364","article-title":"Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles","volume":"23","author":"Wang","year":"2023","journal-title":"Proteomics"},{"key":"10.1016\/j.chroma.2026.467505_bib0029","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1038\/s41592-020-01034-x","article-title":"Exosome detection via the ultrafast-isolation system: EXODUS","volume":"18","author":"Chen","year":"2021","journal-title":"Nat. Methods"},{"key":"10.1016\/j.chroma.2026.467505_bib0030","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12266","article-title":"Comparative analysis of tangential flow filtration and ultracentrifugation, both combined with subsequent size exclusion chromatography, for the isolation of small extracellular vesicles","volume":"11","author":"Visan","year":"2022","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.chroma.2026.467505_bib0031","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2024.116349","article-title":"Bilateral efforts to improve SERS detection efficiency of exosomes by Au\/Na7PMo11O39 combined with phospholipid epitope imprinting","volume":"258","author":"Zhao","year":"2024","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.chroma.2026.467505_bib0032","doi-asserted-by":"crossref","first-page":"4889","DOI":"10.1021\/acs.analchem.4c04269","article-title":"High-efficiency capture and proteomic analysis of plasma-derived extracellular vesicles through affinity purification","volume":"97","author":"Zhang","year":"2025","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2026.467505_bib0033","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2021.107814","article-title":"The yin and yang of exosome isolation methods: conventional practice, microfluidics, and commercial kits","volume":"54","author":"Shirejini","year":"2022","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.chroma.2026.467505_bib0034","doi-asserted-by":"crossref","first-page":"11652","DOI":"10.1039\/D0RA00263A","article-title":"Advances in passively driven microfluidics and lab-on-chip devices: a comprehensive literature review and patent analysis","volume":"10","author":"Narayanamurthy","year":"2020","journal-title":"RSC Adv."},{"key":"10.1016\/j.chroma.2026.467505_bib0035","doi-asserted-by":"crossref","DOI":"10.1002\/smll.202004492","article-title":"Accurate cancer diagnosis and stage monitoring enabled by comprehensive profiling of different types of exosomal biomarkers: surface proteins and miRNAs","volume":"16","author":"Zhou","year":"2020","journal-title":"Small"},{"key":"10.1016\/j.chroma.2026.467505_bib0036","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2025.139170","article-title":"Size-selective separation of extracellular vesicles in culture medium via optically-induced dielectrophoresis on a multi-channel microfluidic chip","volume":"449","author":"Chung","year":"2026","journal-title":"Sens. Actuators B"},{"key":"10.1016\/j.chroma.2026.467505_bib0037","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.202500439","article-title":"Si microanemones integrated microfluidic chip for highly efficient isolation of extracellular vesicles","volume":"14","author":"Kang","year":"2025","journal-title":"Adv. Healthc. Mater."},{"key":"10.1016\/j.chroma.2026.467505_bib0038","doi-asserted-by":"crossref","first-page":"4357","DOI":"10.1039\/D5LC00511F","article-title":"A urine detection chip for the analysis of urinary cells and extracellular vesicles for bladder cancer screening","volume":"25","author":"Liu","year":"2025","journal-title":"Lab Chip"},{"key":"10.1016\/j.chroma.2026.467505_bib0039","doi-asserted-by":"crossref","first-page":"1891","DOI":"10.1039\/C4LC00136B","article-title":"Microfluidic device (ExoChip) for on-chip isolation, quantification and characterization of circulating exosomes","volume":"14","author":"Kanwar","year":"2014","journal-title":"Lab Chip"},{"key":"10.1016\/j.chroma.2026.467505_bib0040","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-024-62783-9","article-title":"Improved recovery of urinary small extracellular vesicles by differential ultracentrifugation","volume":"14","author":"Teixeira-Marques","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.chroma.2026.467505_bib0041","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/s12017-021-08696-6","article-title":"Exosomes derived neuronal markers: immunoaffinity isolation and characterization","volume":"24","author":"Yousif","year":"2022","journal-title":"NeuroMol. Med."},{"key":"10.1016\/j.chroma.2026.467505_bib0042","doi-asserted-by":"crossref","DOI":"10.1002\/smtd.202400426","article-title":"Cellulose nanofibrils of high immunoaffinity for efficient enrichment of small extracellular vesicles","volume":"8","author":"Wang","year":"2024","journal-title":"Small Methods"},{"key":"10.1016\/j.chroma.2026.467505_bib0043","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.nbt.2015.09.003","article-title":"Development of a magnetic bead-based method for the collection of circulating extracellular vesicles","volume":"33","author":"Shih","year":"2016","journal-title":"New Biotechnol."},{"key":"10.1016\/j.chroma.2026.467505_bib0044","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2023.341426","article-title":"Ti3C2 and Ti2C MXene materials for high-performance isolation of extracellular vesicles via coprecipitation","volume":"1269","author":"Chen","year":"2023","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.chroma.2026.467505_bib0045","doi-asserted-by":"crossref","DOI":"10.1016\/j.jenvman.2025.126135","article-title":"In situ construction of green ZnFe2O4\/sub-5nm N, Cu dual-doped SnO2 S-scheme heterostructure with the boosted spatial charge separation towards decontamination of tetracycline: mechanistic perspectives and aquatic hazard assessment","volume":"389","author":"Nateq","year":"2025","journal-title":"J. Environ. Manag."},{"key":"10.1016\/j.chroma.2026.467505_bib0046","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2024.125790","article-title":"Bifunctional immunoaffinity magnetic nanoparticles for high-efficiency separation of exosomes based on host-guest interaction","volume":"272","author":"Pei","year":"2024","journal-title":"Talanta"},{"key":"10.1016\/j.chroma.2026.467505_bib0047","doi-asserted-by":"crossref","first-page":"319","DOI":"10.34133\/cbsystems.0319","article-title":"An acoustofluidic device for sample preparation and detection of small extracellular vesicles","volume":"6","author":"Liu","year":"2025","journal-title":"Cyborg. Bionic. Syst."},{"key":"10.1016\/j.chroma.2026.467505_bib0048","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1021\/acsnanomed.5c00108","article-title":"Negative surface charge and membrane lipid composition underlying extracellular vesicle function","volume":"1","author":"Seo","year":"2026","journal-title":"ACS Nano Med."},{"key":"10.1016\/j.chroma.2026.467505_bib0049","doi-asserted-by":"crossref","first-page":"16701","DOI":"10.1021\/acsomega.0c01582","article-title":"Zeta potential of extracellular vesicles: toward understandingthe attributes that determine colloidal stability","volume":"5","author":"Midekessa","year":"2020","journal-title":"ACS Omega"},{"key":"10.1016\/j.chroma.2026.467505_bib0050","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2025.160850","article-title":"Efficient purification of exosomes and subpopulations in fresh bovine milk via fine isolation of free-flow isoelectric focusing in large scale","volume":"508","author":"Khan","year":"2025","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.chroma.2026.467505_bib0051","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12128","article-title":"Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin","volume":"10","author":"Veerman","year":"2021","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.chroma.2026.467505_bib0052","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1093\/jb\/mvaf052","article-title":"Commentary for the incorporation of extracellular vesicle markers varies among vesicles with distinct surface charges","volume":"178","author":"Matsuzawa","year":"2025","journal-title":"J. Biochem."},{"key":"10.1016\/j.chroma.2026.467505_bib0053","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.70024","article-title":"Enhanced proteomic profiling of human plasma-derived extracellular vesicles through charge-based fractionation to advance biomarker discovery potential","volume":"13","author":"Su","year":"2024","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.chroma.2026.467505_bib0054","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2021.113589","article-title":"Molecular profiling of extracellular vesicles via charge-based capture using oxide nanowire microfluidics","volume":"194","author":"Yasui","year":"2021","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.chroma.2026.467505_bib0055","doi-asserted-by":"crossref","DOI":"10.20517\/evcna.2021.26","article-title":"Free flow electrophoresis allows quick and reproducible preparation of extracellular vesicles from conditioned cell culture media","author":"Staubach","year":"2022","journal-title":"Extracell. Vesicles Circ. Nucleic Acids"},{"key":"10.1016\/j.chroma.2026.467505_bib0056","doi-asserted-by":"crossref","DOI":"10.1002\/jex2.71","article-title":"Isolation of native EVs from primary biofluids\u2014free-flow electrophoresis as a novel approach to purify ascites-derived EVs","volume":"1","author":"Preu\u00dfer","year":"2022","journal-title":"J. Extracell. Biol."},{"key":"10.1016\/j.chroma.2026.467505_bib0057","doi-asserted-by":"crossref","first-page":"S57","DOI":"10.1016\/j.jcyt.2019.03.432","article-title":"Free flow electrophoresis allows preparation of extracellular vesicles with high purity","volume":"21","author":"Staubach","year":"2019","journal-title":"Cytotherapy"},{"key":"10.1016\/j.chroma.2026.467505_bib0058","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2022.340306","article-title":"Free-flow zone electrophoresis facilitated proteomics analysis of heterogeneous subpopulations in H1299 lung cancer cells","volume":"1227","author":"Sohail","year":"2022","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.chroma.2026.467505_bib0059","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.aca.2017.06.007","article-title":"Preparation of intact mitochondria using free-flow isoelectric focusing with post-pH gradient sample injection for morphological, functional and proteomics studies","volume":"982","author":"He","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.chroma.2026.467505_bib0060","doi-asserted-by":"crossref","first-page":"12017","DOI":"10.1021\/acs.analchem.0c02627","article-title":"Free-flow isoelectric focusing for comprehensive separation and analysis of human salivary microbiome for lung cancer","volume":"92","author":"Jiang","year":"2020","journal-title":"Anal. Chem."},{"key":"10.1016\/j.chroma.2026.467505_bib0061","doi-asserted-by":"crossref","first-page":"2928","DOI":"10.1016\/j.chroma.2008.07.093","article-title":"Development of a simple ampholyte-free isoelectric focusing slab electrophoresis for protein fractionation","volume":"1216","author":"Zhan","year":"2009","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.chroma.2026.467505_bib0062","doi-asserted-by":"crossref","first-page":"2610","DOI":"10.1002\/elps.201900148","article-title":"Carrier ampholyte-free free-flow isoelectric focusing for separation of protein","volume":"40","author":"Wang","year":"2019","journal-title":"Electrophoresis"},{"key":"10.1016\/j.chroma.2026.467505_bib0063","doi-asserted-by":"crossref","DOI":"10.1016\/j.aca.2025.344567","article-title":"Ampholyte-free microfluidic free-flow isoelectric focusing via ion-exchange membranes for particle separation","volume":"1375","author":"Yoon","year":"2025","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.chroma.2026.467505_bib0064","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1021\/acsnano.5b05406","article-title":"Full-color light-emitting carbon dots with a surface-State-controlled luminescence mechanism","volume":"10","author":"Ding","year":"2016","journal-title":"ACS Nano"},{"key":"10.1016\/j.chroma.2026.467505_bib0065","doi-asserted-by":"crossref","DOI":"10.1002\/smll.202001909","article-title":"Photo-stimulated polychromatic room temperature phosphorescence of carbon dots","volume":"16","author":"Jiang","year":"2020","journal-title":"Small"},{"key":"10.1016\/j.chroma.2026.467505_bib0066","doi-asserted-by":"crossref","DOI":"10.1016\/j.talanta.2025.128412","article-title":"Semiquantitative analysis of carbon quantum dot-labeled exosomes based on carrier ampholyte-free paper-based isoelectric focusing","volume":"296","author":"Liu","year":"2026","journal-title":"Talanta"},{"key":"10.1016\/j.chroma.2026.467505_bib0067","first-page":"121","article-title":"Oxytetracycline nanosensor based on poly-ortho-aminophenol\/multi-walled carbon nanotubes composite film, Iran","volume":"23","author":"Ajami","year":"2014","journal-title":"Polym. J."},{"key":"10.1016\/j.chroma.2026.467505_bib0068","doi-asserted-by":"crossref","first-page":"5885","DOI":"10.1039\/D3AY01708G","article-title":"Separation and purification of active ingredients in tobacco by free-flow electrophoresis","volume":"15","author":"Sha","year":"2023","journal-title":"Anal. Methods"},{"key":"10.1016\/j.chroma.2026.467505_bib0069","series-title":"Frontiers in Voltammetry","article-title":"A new method for determination of molecular weight of compounds soluble in protic solvents by electrochemical impedance spectroscopy","author":"Rameshkumar","year":"2023"},{"key":"10.1016\/j.chroma.2026.467505_bib0070","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1007\/s00604-024-06864-6","article-title":"A simple capillary isoelectric focusing method as the novel strategy for the isoelectric point measurement of exosomes and its application in disease diagnosis","volume":"192","author":"Yang","year":"2025","journal-title":"Microchim. Acta"},{"key":"10.1016\/j.chroma.2026.467505_bib0071","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.1039\/C8SC04197K","article-title":"A novel strategy for facile serum exosome isolation based on specific interactions between phospholipid bilayers and TiO2","volume":"10","author":"Gao","year":"2019","journal-title":"Chem. Sci."},{"key":"10.1016\/j.chroma.2026.467505_bib0072","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1007\/s00604-021-04934-7","article-title":"Rapid and specific detection nanoplatform of serum exosomes for prostate cancer diagnosis","volume":"188","author":"Li","year":"2021","journal-title":"Microchim. Acta"},{"key":"10.1016\/j.chroma.2026.467505_bib0073","doi-asserted-by":"crossref","DOI":"10.1016\/j.jwpe.2025.108878","article-title":"Enhanced cephalosporin antibiotics degradation through simultaneous electrooxidation-elecrocoagulation treatment using IrO\u2082-RuO\u2082-TiO\u2082-ta\u2082o\u2085 mixed metal oxide anode","volume":"79","author":"Danaee","year":"2025","journal-title":"J. Water Process. Eng."},{"key":"10.1016\/j.chroma.2026.467505_bib0074","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1186\/s12014-026-09615-2","article-title":"Comparative mass spectrometry analysis of high and low centrifugation extracellular vesicle (EV) pellets from healthy urine following tamm-horsfall protein removal","volume":"23","author":"Bielopolski","year":"2026","journal-title":"Clin. Proteom."},{"key":"10.1016\/j.chroma.2026.467505_bib0075","doi-asserted-by":"crossref","first-page":"2217","DOI":"10.1021\/acs.jproteome.9b00693","article-title":"Characterizationof urinary exosomes purified withSize exclusion chromatography and ultracentrifugation","volume":"19","author":"Guan","year":"2020","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.chroma.2026.467505_bib0076","doi-asserted-by":"crossref","DOI":"10.1016\/j.jchromb.2023.123828","article-title":"Size-exclusion chromatography for the characterization of urinary extracellular vesicles","volume":"1228","author":"Park","year":"2023","journal-title":"J. Chromatogr. B"},{"key":"10.1016\/j.chroma.2026.467505_bib0077","doi-asserted-by":"crossref","first-page":"4881","DOI":"10.3390\/ijms22094881","article-title":"Size-exclusion chromatography separation reveals that vesicular and non-vesicular small RNA profiles differ in cell free urine","volume":"22","author":"Karttunen","year":"2021","journal-title":"Int. J. Mol. Sci."}],"container-title":["Journal of Chromatography A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967326008319?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021967326008319?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T12:06:07Z","timestamp":1791633967000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021967326008319"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":77,"alternative-id":["S0021967326008319"],"URL":"https:\/\/doi.org\/10.1016\/j.chroma.2026.467505","relation":{},"ISSN":["0021-9673"],"issn-type":[{"value":"0021-9673","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Visualized separation of exosomes in urine using carrier ampholyte-free free-flow isoelectric focusing electrophoresis","name":"articletitle","label":"Article Title"},{"value":"Journal of Chromatography A","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.chroma.2026.467505","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"467505"}}