{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:37:22Z","timestamp":1791333442936,"version":"4.3.1"},"reference-count":39,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Comparative Immunology, Microbiology and Infectious Diseases"],"published-print":{"date-parts":[[2012,3]]},"DOI":"10.1016\/j.cimid.2011.12.010","type":"journal-article","created":{"date-parts":[[2012,1,17]],"date-time":"2012-01-17T23:22:48Z","timestamp":1326842568000},"page":"173-179","source":"Crossref","is-referenced-by-count":7,"title":["Development and validation with clinical samples of internally controlled multiplex real-time PCR for diagnosis of BKV and JCV infection in associated pathologies"],"prefix":"10.1016","volume":"35","author":[{"given":"Ana","family":"B\u00e1rcena-Panero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan E.","family":"Echevarr\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Pilar","family":"Romero-G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrique","family":"Royuela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Castellanos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Fedele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cimid.2011.12.010_bib0005","doi-asserted-by":"crossref","first-page":"e1000363","DOI":"10.1371\/journal.ppat.1000363","article-title":"Seroepidemiology of human polyomaviruses","volume":"5","author":"Kean","year":"2009","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.cimid.2011.12.010_bib0010","first-page":"42","article-title":"Occurrence and significance of papovaviruses BK and JC in the urine","volume":"36","author":"Arthur","year":"1989","journal-title":"Prog Med Virol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0015","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1006\/viro.1994.1008","article-title":"Infection of human polyomaviruses JC and BK in peripheral blood leukocytes from immunocompetent individuals","volume":"198","author":"Dorries","year":"1994","journal-title":"Virology"},{"key":"10.1016\/j.cimid.2011.12.010_bib0020","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1002\/ana.410310426","article-title":"Detection of JC virus DNA in peripheral lymphocytes from patients with and without progressive multifocal leukoencephalopathy","volume":"31","author":"Tornatore","year":"1992","journal-title":"Ann Neurol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0025","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1097\/00042560-199606010-00006","article-title":"JCV-DNA and BKV-DNA in the CNS tissue and CSF of AIDS patients and normal subjects. Study of 41 cases and review of the literature","volume":"12","author":"Vago","year":"1996","journal-title":"J Acquir Immune Defic Syndr Hum Retrovirol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0030","doi-asserted-by":"crossref","first-page":"2625","DOI":"10.1099\/0022-1317-81-11-2625","article-title":"VP1 DNA sequences of JC and BK viruses detected in urine of systemic lupus erythematosus patients reveal no differences from strains expressed in normal individuals","volume":"81","author":"Bendiksen","year":"2000","journal-title":"J Gen Virol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0035","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1023\/B:VIRU.0000016854.37475.f3","article-title":"High reactivation of BK virus variants in Asian Indians with renal disorders and during pregnancy","volume":"28","author":"Bhattacharjee","year":"2004","journal-title":"Virus Genes"},{"key":"10.1016\/j.cimid.2011.12.010_bib0040","doi-asserted-by":"crossref","first-page":"4515","DOI":"10.1128\/JVI.65.8.4515-4519.1991","article-title":"BK virus and JC virus shed during pregnancy have predominantly archetypal regulatory regions","volume":"65","author":"Markowitz","year":"1991","journal-title":"J Virol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0045","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1128\/JCM.01645-06","article-title":"Age-related urinary excretion of BK polyomavirus by nonimmunocompromised individuals","volume":"45","author":"Zhong","year":"2007","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0050","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/sj.bmt.1705886","article-title":"Polyomavirus BK infection in blood and marrow transplant recipients","volume":"41","author":"Dropulic","year":"2008","journal-title":"Bone Marrow Transplant"},{"key":"10.1016\/j.cimid.2011.12.010_bib0055","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1097\/TP.0b013e318197c17d","article-title":"The decade of polyomavirus BK-associated nephropathy: state of affairs","volume":"87","author":"Ramos","year":"2009","journal-title":"Transplantation"},{"key":"10.1016\/j.cimid.2011.12.010_bib0060","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1097\/01.TP.0000156165.83160.09","article-title":"Polyomavirus-associated nephropathy in renal transplantation: interdisciplinary analyses and recommendations","volume":"79","author":"Hirsch","year":"2005","journal-title":"Transplantation"},{"key":"10.1016\/j.cimid.2011.12.010_bib0065","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1146\/annurev.med.080708.082655","article-title":"Progressive multifocal leukoencephalopathy in patients on immunomodulatory therapies","volume":"61","author":"Major","year":"2010","journal-title":"Annu Rev Med"},{"issue":"Suppl. 3","key":"10.1016\/j.cimid.2011.12.010_bib0070","doi-asserted-by":"crossref","first-page":"iii48","DOI":"10.1136\/ard.2006.058404","article-title":"Natalizumab and progressive multifocal leucoencephalopathy","volume":"65","author":"Berger","year":"2006","journal-title":"Ann Rheum Dis"},{"key":"10.1016\/j.cimid.2011.12.010_bib0075","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1097\/00002030-199906180-00010","article-title":"BK virus as the cause of meningoencephalitis, retinitis and nephritis in a patient with AIDS","volume":"13","author":"Bratt","year":"1999","journal-title":"AIDS"},{"key":"10.1016\/j.cimid.2011.12.010_bib0080","first-page":"29","article-title":"Disseminated BK type polyomavirus infection in an AIDS patient associated with central nervous system disease","volume":"143","author":"Vallbracht","year":"1993","journal-title":"Am J Pathol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0085","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1001\/archneur.1996.00550010121025","article-title":"BK virus encephalitis in an immunocompetent patient","volume":"53","author":"Voltz","year":"1996","journal-title":"Arch Neurol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0090","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/S0035-3787(05)85040-4","article-title":"Progressive BK virus associated multifocal leukoencephalopathy in an immunocompromised patient treated with corticosteroids","volume":"161","author":"Cabrejo","year":"2005","journal-title":"Rev Neurol (Paris)"},{"key":"10.1016\/j.cimid.2011.12.010_bib0095","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1097\/01.TP.0000137106.09925.8B","article-title":"Delirium in a renal transplant recipient associated with BK virus in the cerebrospinal fluid","volume":"78","author":"Hix","year":"2004","journal-title":"Transplantation"},{"key":"10.1016\/j.cimid.2011.12.010_bib0100","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1097\/01.tp.0000269706.59977.a5","article-title":"Polyomavirus BK versus JC replication and nephropathy in renal transplant recipients: a prospective evaluation","volume":"84","author":"Drachenberg","year":"2007","journal-title":"Transplantation"},{"key":"10.1016\/j.cimid.2011.12.010_bib0105","doi-asserted-by":"crossref","first-page":"1653","DOI":"10.1097\/01.TP.0000090749.42791.14","article-title":"The first case of JC virus allograft nephropathy","volume":"76","author":"Kazory","year":"2003","journal-title":"Transplantation"},{"key":"10.1016\/j.cimid.2011.12.010_bib0110","first-page":"104","article-title":"Hemorrhagic cystitis caused by BK and JC polyomavirus in patients treated with bone marrow transplantation: clinical features and urologic management","volume":"26","author":"Garcia Ligero","year":"2002","journal-title":"Actas Urol Esp"},{"key":"10.1016\/j.cimid.2011.12.010_bib0115","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1016\/j.bbmt.2005.06.007","article-title":"Incidence, clinical outcome, and management of virus-induced hemorrhagic cystitis in children and adolescents after allogeneic hematopoietic cell transplantation","volume":"11","author":"Gorczynska","year":"2005","journal-title":"Biol Blood Marrow Transplant"},{"key":"10.1016\/j.cimid.2011.12.010_bib0120","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1002\/jmv.21558","article-title":"Viral infection in bone marrow transplants: is JC virus involved?","volume":"82","author":"Mischitelli","year":"2010","journal-title":"J Med Virol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0125","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0166-0934(99)00095-6","article-title":"Multiplex polymerase chain reaction for the simultaneous detection and typing of polyomavirus JC, BK and SV40 DNA in clinical samples","volume":"82","author":"Fedele","year":"1999","journal-title":"J Virol Methods"},{"key":"10.1016\/j.cimid.2011.12.010_bib0130","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1002\/jcp.22711","article-title":"BK virus-associated infection in cerebrospinal fluid of neurological patients and mutation analysis of the complete VP1 gene in different patient groups","volume":"227","author":"B\u00e1rcena-Panero","year":"2012","journal-title":"J Cell Physiol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0135","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/S0166-0934(97)00035-9","article-title":"Detection of enteroviral RNA and specific DNA of herpesviruses by multiplex genome amplification","volume":"66","author":"Casas","year":"1997","journal-title":"J Virol Methods"},{"key":"10.1016\/j.cimid.2011.12.010_bib0140","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1128\/JCM.38.1.357-361.2000","article-title":"Improved amplification of genital human papillomaviruses","volume":"38","author":"Gravitt","year":"2000","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0145","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0166-0934(00)00172-5","article-title":"Quantitation of polyomavirus DNA by a competitive nested polymerase chain reaction","volume":"88","author":"Fedele","year":"2000","journal-title":"J Virol Methods"},{"key":"10.1016\/j.cimid.2011.12.010_bib0150","doi-asserted-by":"crossref","first-page":"2285","DOI":"10.1128\/JVI.02180-08","article-title":"Genotyping schemes for polyomavirus BK, using gene-specific phylogenetic trees and single nucleotide polymorphism analysis","volume":"83","author":"Luo","year":"2009","journal-title":"J Virol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0155","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1002\/jmv.21710","article-title":"Characterization of JC human polyomavirus infection in a Portuguese population","volume":"82","author":"Matos","year":"2010","journal-title":"J Med Virol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0160","doi-asserted-by":"crossref","first-page":"9928","DOI":"10.1128\/JVI.00441-06","article-title":"JC virus evolution and its association with human populations","volume":"80","author":"Shackelton","year":"2006","journal-title":"J Virol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0165","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1099\/vir.0.83611-0","article-title":"Distribution patterns of BK polyomavirus (BKV) subtypes and subgroups in American, European and Asian populations suggest co-migration of BKV and the human race","volume":"90","author":"Zhong","year":"2009","journal-title":"J Gen Virol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0170","doi-asserted-by":"crossref","first-page":"2829","DOI":"10.1128\/JCM.00844-08","article-title":"Internally controlled triplex quantitative PCR assay for human polyomaviruses JC and BK","volume":"46","author":"Dumonceaux","year":"2008","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0175","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1111\/j.1198-743X.2004.00722.x","article-title":"Real-time PCR in the microbiology laboratory","volume":"10","author":"Mackay","year":"2004","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.cimid.2011.12.010_bib0180","doi-asserted-by":"crossref","first-page":"3379","DOI":"10.1128\/AEM.02302-08","article-title":"Quantification of human polyomaviruses JC Virus and BK virus by TaqMan quantitative PCR and comparison to other water quality indicators in water and fecal samples","volume":"75","author":"McQuaig","year":"2009","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0185","first-page":"375","article-title":"Absence of simian virus 40, BK, and JC polyomavirus DNA in squamous cell carcinoma limited to the oral cavity","volume":"32","author":"Palmieri","year":"2010","journal-title":"Head Neck"},{"key":"10.1016\/j.cimid.2011.12.010_bib0190","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/S1474-4422(10)70028-4","article-title":"Natalizumab-associated progressive multifocal leukoencephalopathy in patients with multiple sclerosis: lessons from 28 cases","volume":"9","author":"Clifford","year":"2010","journal-title":"Lancet Neurol"},{"key":"10.1016\/j.cimid.2011.12.010_bib0195","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1073\/pnas.50.4.730","article-title":"The cyclic helix and cyclic coil forms of polyoma viral DNA","volume":"50","author":"Weil","year":"1963","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["Comparative Immunology, Microbiology and Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147957111001299?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147957111001299?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,12,26]],"date-time":"2021-12-26T08:26:37Z","timestamp":1640507197000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0147957111001299"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3]]},"references-count":39,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["S0147957111001299"],"URL":"https:\/\/doi.org\/10.1016\/j.cimid.2011.12.010","relation":{},"ISSN":["0147-9571"],"issn-type":[{"value":"0147-9571","type":"print"}],"subject":[],"published":{"date-parts":[[2012,3]]}}}