{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T13:31:28Z","timestamp":1782135088664,"version":"3.54.5"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"CAPES for granting a masters scholarship and the S\u00e3o Paulo Research Foundation, FAPESP","award":["2013\/06684-9"],"award-info":[{"award-number":["2013\/06684-9"]}]}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Comparative Immunology, Microbiology and Infectious Diseases"],"published-print":{"date-parts":[[2018,4]]},"DOI":"10.1016\/j.cimid.2018.01.003","type":"journal-article","created":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T14:15:36Z","timestamp":1517494536000},"page":"1-7","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis"],"prefix":"10.1016","volume":"57","author":[{"given":"Cleber Costa","family":"de Martini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ssica Thom\u00e9","family":"de Andrade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9fani Karin Martiniano","family":"de Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathlenn Liezbeth","family":"Oliveira Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia","family":"de Rezende Eugenio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo Sergio Patto","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9ria Mar\u00e7al Felix","family":"de Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cimid.2018.01.003_bib0005","doi-asserted-by":"crossref","first-page":"e35671","DOI":"10.1371\/journal.pone.0035671","article-title":"WHO Leishmaniasis Control Team. Leishmaniasis worldwide and global estimates of its incidence","volume":"7","author":"Alvar","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.cimid.2018.01.003_bib0010","series-title":"Leishmaniasis Report","article-title":"Leishmaniasis epidemiological report in the Americas","author":"Organization","year":"2018"},{"key":"10.1016\/j.cimid.2018.01.003_bib0015","doi-asserted-by":"crossref","first-page":"5460","DOI":"10.1158\/1078-0432.CCR-11-3368","article-title":"Vaccination with mRNA-electroporated dendritic cells induces robust tumor antigen-specific CD4+ and CD8+ T cells responses in stage III and IV melanoma patients","volume":"18","author":"Aarntzen","year":"2012","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.cimid.2018.01.003_bib0020","doi-asserted-by":"crossref","first-page":"e0159240","DOI":"10.1371\/journal.pone.0159240","article-title":"Biodiversity and temporal distribution of immature culicidae in the Atlantic Forest, Rio de Janeiro State, Brazil","volume":"11","author":"Alencar","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.cimid.2018.01.003_bib0025","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1111\/j.1365-3024.2008.01051.x","article-title":"Inflammation and structural changes of splenic lymphoid tissue in visceral leishmaniasis: a study on naturally infected dogs","volume":"30","author":"Santana","year":"2008","journal-title":"Parasite Immunol."},{"key":"10.1016\/j.cimid.2018.01.003_bib0030","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1111\/j.1939-1676.2005.tb02680.x","article-title":"Hereditary spectrin deficiency in Golden Retriever dogs","volume":"19","author":"Slappendel","year":"2005","journal-title":"J. Vet. Intern. Med."},{"key":"10.1016\/j.cimid.2018.01.003_bib0035","unstructured":"Institute Adolfo Lutz. Regional Laboratory Center of Ara\u00e7atuba, SP. In: Canine visceral leishmaniasis inquiry data. http:\/\/www.ial.sp.gov.br\/2016."},{"key":"10.1016\/j.cimid.2018.01.003_bib0040","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1638\/2012-0059R1.1","article-title":"Molecular diagnosis of Leishmania amazonensis in a captive spider monkey in Bauru, S\u00e3o Paulo, Brazil","volume":"43","author":"Lima","year":"2012","journal-title":"J. Zoo Wildl. Med."},{"key":"10.1016\/j.cimid.2018.01.003_bib0045","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.vetpar.2014.09.006","article-title":"Alteration of sFAS and sFAS ligand expression during canine visceral leishmaniosis","volume":"205","author":"Perosso","year":"2014","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.cimid.2018.01.003_bib0050","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1007\/s00436-010-2183-5","article-title":"Qualitative and quantitative immunohistochemical evaluation of iNOS expression in the spleen of dogs naturally infected with Leishmania chagasi","volume":"108","author":"dos Santos","year":"2011","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.cimid.2018.01.003_bib0055","doi-asserted-by":"crossref","first-page":"e43","DOI":"10.1016\/j.tvjl.2011.04.005","article-title":"Humoral and cellular immune responses in dogs with inapparent natural Leishmania infantum infection","volume":"190","author":"Coura-Vital","year":"2011","journal-title":"Vet. J."},{"key":"10.1016\/j.cimid.2018.01.003_bib0060","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/j.1365-2249.2006.03206.x","article-title":"Phenotypic features of circulating leucocytes as immunological markers for clinical status and bone marrow parasite density in dogs naturally infected by Leishmania chagasi","volume":"146","author":"Reis","year":"2006","journal-title":"Clin. Exp. Immunol."},{"key":"10.1016\/j.cimid.2018.01.003_bib0065","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.vetpar.2010.11.025","article-title":"Cytokine and transcription factor profiles in the skin of dogs naturally infected by Leishmania (Leishmania) chagasi presenting distinct cutaneous parasite density and clinical status","volume":"177","author":"Menezes-Souza","year":"2011","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.cimid.2018.01.003_bib0070","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S0304-4017(00)00312-5","article-title":"Infection of a canine macrophage cell line with Leishmania infantum: determination of nitric oxide production and anti-leishmanial activity","volume":"92","author":"Pinelli","year":"2000","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.cimid.2018.01.003_bib0075","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/1756-3305-1-10","article-title":"Canine leishmaniasis in Southern Italy: a role for nitric oxide released from activated macrophages in asymptomatic infection","volume":"1","author":"Panaro","year":"2008","journal-title":"Parasite Vectors"},{"key":"10.1016\/j.cimid.2018.01.003_bib0080","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1038\/ni1001-907","article-title":"Nitric oxide and the immune response","volume":"2","author":"Bogdan","year":"2001","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.cimid.2018.01.003_bib0085","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1038\/sj.cdd.4401261","article-title":"Nitric oxide: NO apoptosis or turning it on","volume":"10","author":"Br\u00fcne","year":"2003","journal-title":"Cell Death Differ."},{"key":"10.1016\/j.cimid.2018.01.003_bib0090","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.vetpar.2013.07.012","article-title":"CD95(FAS) and CD178 (FASL) induce the apoptosis of CD4+ and CD8+ cells isolated from the peripheral blood and spleen of dogs naturally infected with Leishmania spp","volume":"197","author":"Silva","year":"2013","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.cimid.2018.01.003_bib0095","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1016\/j.imbio.2016.03.007","article-title":"PD-1 function in apoptosis of T lymphocytes in canine visceral leishmaniasis","volume":"221","author":"Chiku","year":"2016","journal-title":"Immunobiology"},{"key":"10.1016\/j.cimid.2018.01.003_bib0100","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1096\/fj.01-0569fje","article-title":"Relaxin up-regulates inducible nitric oxide synthase expression and nitric oxide generation in rat coronary endothelial cells","volume":"16","author":"Failli","year":"2002","journal-title":"FASEB J."},{"key":"10.1016\/j.cimid.2018.01.003_bib0105","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1093\/ajcp\/100.5.567","article-title":"Nitric oxide. Biochemistry pathophysiology, and detection","volume":"100","author":"Kiechle","year":"1993","journal-title":"Am. J. Clin. Pathol."},{"key":"10.1016\/j.cimid.2018.01.003_bib0110","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/S0076-6879(02)59178-3","article-title":"Detection of nitric oxide production by fluorescent indicators","volume":"359","author":"Chatton","year":"2002","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.cimid.2018.01.003_bib0115","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1002\/cyto.a.21001","article-title":"Monitoring of intracellular nitric oxide in leishmaniasis: its applicability in patients with visceral leishmaniasis","volume":"79","author":"Sarkar","year":"2011","journal-title":"Cytometry A"},{"key":"10.1016\/j.cimid.2018.01.003_bib0120","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1590\/S0100-879X2003000400010","article-title":"Anti-Leishmania antibodies in cerebrospinal fluid from dogs with visceral leishmaniasis","volume":"36","author":"Lima","year":"2003","journal-title":"Braz. J. Med. Biol. Res."},{"key":"10.1016\/j.cimid.2018.01.003_bib0125","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.vetimm.2008.02.022","article-title":"High iNOS expression in macrophages in canine leishmaniasis is associated with low intracellular parasite burden","volume":"123","author":"Zafra","year":"2008","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.cimid.2018.01.003_bib0130","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/s13028-014-0057-z","article-title":"Expression of inducible nitric oxide synthase in macrophages inversely correlates with parasitism of lymphoid tissues in dogs with visceral leishmaniasis","volume":"56","author":"Sanches","year":"2014","journal-title":"Acta Vet. Scand."},{"key":"10.1016\/j.cimid.2018.01.003_bib0135","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.jcpa.2015.10.174","article-title":"Impairment of interleukin -17A expression in canine visceral leishmaniosis is correlated with reduced interferon-\u00e3 and inducible nitric oxide synthase expression","author":"Nascimento","year":"2015","journal-title":"J. Comp. Pathol."},{"key":"10.1016\/j.cimid.2018.01.003_bib0140","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1017\/S0031182012001187","article-title":"Alteration in mononuclear cell subpopulations in dogs immunized with gentamicin-attenuated Leishmania infantum","volume":"139","author":"Daneshvar","year":"2012","journal-title":"Parasitology"},{"key":"10.1016\/j.cimid.2018.01.003_bib0145","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.vetpar.2014.08.022","article-title":"Immunological profile of resistance and susceptibility in naturally infected dogs by Leishmania infantum","volume":"205","author":"de Almeida Leal","year":"2014","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.cimid.2018.01.003_bib0150","doi-asserted-by":"crossref","first-page":"3170","DOI":"10.1182\/blood.V82.10.3170.3170","article-title":"The novel subset of CD14+\/CD16+ blood monocytes is expanded in sepsis patients","volume":"82","author":"Fingerle","year":"1993","journal-title":"Blood"},{"key":"10.1016\/j.cimid.2018.01.003_bib0155","doi-asserted-by":"crossref","first-page":"2782","DOI":"10.1128\/IAI.66.6.2782-2790.1998","article-title":"Expanded CD14+ CD16+ monocyte subpopulation in patients with acute and chronic infections undergoing hemodialysis","volume":"66","author":"Nockher","year":"1998","journal-title":"Infect. Immun."},{"key":"10.1016\/j.cimid.2018.01.003_bib0160","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/1471-2172-14-22","article-title":"Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum","volume":"14","author":"Figueiredo","year":"2013","journal-title":"BMC. Immunol."},{"key":"10.1016\/j.cimid.2018.01.003_bib0165","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/1756-3305-7-61","article-title":"LBSapSal-vaccinated dogs exhibit increased circulating T-lymphocyte subsets (CD4\u207a and CD8\u207a) as well as a reduction of parasitism after challenge with Leishmania infantum plus salivary gland of Lutzomyia longipalpis","volume":"7","author":"Aguiar-Soares","year":"2014","journal-title":"Parasite Vectors"},{"key":"10.1016\/j.cimid.2018.01.003_bib0170","doi-asserted-by":"crossref","DOI":"10.1111\/pim.12493","article-title":"Dog skin parasite load, TLR-2, IL-10 and TNF-\u03b1 expression and infectiousness","volume":"39","author":"Pereira-Fonseca","year":"2017","journal-title":"Parasite Immunol."},{"key":"10.1016\/j.cimid.2018.01.003_bib0175","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.micpath.2004.11.002","article-title":"Immunopathogenesis of infection with the visceralizing Leishmania species","volume":"38","author":"Wilson","year":"2005","journal-title":"Microb. Pathog."}],"container-title":["Comparative Immunology, Microbiology and Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147957118300183?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147957118300183?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,10,26]],"date-time":"2020-10-26T22:31:20Z","timestamp":1603751480000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0147957118300183"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4]]},"references-count":35,"alternative-id":["S0147957118300183"],"URL":"https:\/\/doi.org\/10.1016\/j.cimid.2018.01.003","relation":{},"ISSN":["0147-9571"],"issn-type":[{"value":"0147-9571","type":"print"}],"subject":[],"published":{"date-parts":[[2018,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cellular apoptosis and nitric oxide production in PBMC and spleen from dogs with visceral leishmaniasis","name":"articletitle","label":"Article Title"},{"value":"Comparative Immunology, Microbiology and Infectious Diseases","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cimid.2018.01.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}