{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T03:32:39Z","timestamp":1777433559710,"version":"3.51.4"},"reference-count":34,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran","award":["94\/2-5\/6"],"award-info":[{"award-number":["94\/2-5\/6"]}]}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Comparative Immunology, Microbiology and Infectious Diseases"],"published-print":{"date-parts":[[2018,8]]},"DOI":"10.1016\/j.cimid.2018.09.012","type":"journal-article","created":{"date-parts":[[2018,9,23]],"date-time":"2018-09-23T13:33:33Z","timestamp":1537709613000},"page":"52-56","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Quantification of Toxoplasma gondii in the tissues of BALB\/c mice after immunization with nanoliposomal excretory-secretory antigens using Real-Time PCR"],"prefix":"10.1016","volume":"59","author":[{"given":"Yaghob","family":"Azadi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1202-6147","authenticated-orcid":false,"given":"Ehsan","family":"Ahmadpour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamed","family":"Hamishehkar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8571-5803","authenticated-orcid":false,"given":"Ahmad","family":"Daryani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adel","family":"Spotin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmoud","family":"Mahami-Oskouei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0132-2277","authenticated-orcid":false,"given":"Aleksandra","family":"Barac","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saba","family":"Rajabi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paria","family":"Alizadeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahbobeh","family":"Montazeri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.cimid.2018.09.012_bib0005","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1590\/S0074-02762009000200033","article-title":"Toxoplasma gondii in animals used for human consumption","volume":"104","author":"Tenter","year":"2009","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz."},{"key":"10.1016\/j.cimid.2018.09.012_bib0010","series-title":"Toxoplasmosis of Animals and Humans","author":"Dubey","year":"2016"},{"key":"10.1016\/j.cimid.2018.09.012_bib0015","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.3855\/jidc.4796","article-title":"Toxoplasmosis in immunocompromised patients in Iran: a systematic review and meta-analysis","volume":"8","author":"Ahmadpour","year":"2014","journal-title":"J. Infect. Dev."},{"key":"10.1016\/j.cimid.2018.09.012_bib0020","first-page":"45","article-title":"Clinical and diagnostic management of toxoplasmosis in the immunocompromised patient","volume":"50","author":"Contini","year":"2008","journal-title":"Parassitologia"},{"key":"10.1016\/j.cimid.2018.09.012_bib0025","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1586\/14760584.2013.844652","article-title":"Vaccines against Toxoplasma gondii: new developments and perspectives","volume":"12","author":"Zhang","year":"2013","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.cimid.2018.09.012_bib0030","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1017\/S0031182014000481","article-title":"Veterinary vaccines against toxoplasmosis","volume":"141","author":"Hiszczy\u0144ska-sawicka","year":"2014","journal-title":"Parasitology"},{"key":"10.1016\/j.cimid.2018.09.012_bib0035","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1007\/s12639-014-0546-z","article-title":"The effect of chitosan nanospheres on the immunogenicity of Toxoplasma lysate vaccine in mice","volume":"40","author":"El Temsahy","year":"2016","journal-title":"J. Parasit. Dis."},{"key":"10.1016\/j.cimid.2018.09.012_bib0040","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.imlet.2017.03.006","article-title":"Enhancing immune responses to a DNA vaccine encoding Toxoplasma gondii GRA14 by calcium phosphate nanoparticles as an adjuvant","volume":"185","author":"Ahmadpour","year":"2017","journal-title":"Immunol. Lett."},{"key":"10.1016\/j.cimid.2018.09.012_bib0045","doi-asserted-by":"crossref","first-page":"5496","DOI":"10.1039\/C6TB01131D","article-title":"Nanomaterial-based vaccine adjuvants","volume":"4","author":"Sun","year":"2016","journal-title":"J. Mater. Chem. B"},{"key":"10.1016\/j.cimid.2018.09.012_bib0050","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1177\/2051013614541440","article-title":"Liposomes as vaccine delivery systems: a review of the recent advances","volume":"2","author":"Schwendener","year":"2014","journal-title":"Ther. Adv. Vaccines"},{"key":"10.1016\/j.cimid.2018.09.012_bib0055","doi-asserted-by":"crossref","first-page":"2761","DOI":"10.4161\/hv.29589","article-title":"Applications of nanomaterials as vaccine adjuvants","volume":"10","author":"Zhu","year":"2014","journal-title":"Hum. Vaccine Immunother."},{"key":"10.1016\/j.cimid.2018.09.012_bib0060","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1073\/pnas.89.1.358","article-title":"Liposomal malaria vaccine in humans: a safe and potent adjuvant strategy","volume":"89","author":"Fries","year":"1992","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.cimid.2018.09.012_bib0065","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.4049\/jimmunol.148.5.1585","article-title":"In vivo cytotoxic T lymphocyte induction with soluble proteins administered in liposomes","volume":"148","author":"Reddy","year":"1992","journal-title":"J. Immunol."},{"key":"10.1016\/j.cimid.2018.09.012_bib0070","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.exppara.2014.05.008","article-title":"Determination of parasitic load in different tissues of murine toxoplasmosis after immunization by excretory\u2013secretory antigens using Real time QPCR","volume":"143","author":"Daryani","year":"2014","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.cimid.2018.09.012_bib0075","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.cimid.2014.10.003","article-title":"Excretory\u2013secretory antigens: a suitable candidate for immunization against ocular toxoplasmosis in a murine model","volume":"37","author":"Deilami","year":"2014","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"10.1016\/j.cimid.2018.09.012_bib0080","first-page":"104","article-title":"Comparison of eight cell-free media for maintenance of Toxoplasma gondii tachyzoites","volume":"11","author":"Kalani","year":"2016","journal-title":"Iran. J. Parasitol."},{"key":"10.1016\/j.cimid.2018.09.012_bib0085","first-page":"7","article-title":"Electrophoretic patterns of Toxoplasma gondii excreted\/secreted antigens and their role in induction of the humoral immune response","author":"Daryani","year":"2014","journal-title":"Jundishapur J. Microbiol."},{"key":"10.1016\/j.cimid.2018.09.012_bib0090","doi-asserted-by":"crossref","first-page":"2371","DOI":"10.1128\/iai.65.6.2371-2377.1997","article-title":"Adjuvanticity and protective immunity elicited by Leishmania donovani antigens encapsulated in positively charged liposomes","volume":"65","author":"Afrin","year":"1997","journal-title":"Infect. Immun."},{"key":"10.1016\/j.cimid.2018.09.012_bib0095","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.cimid.2018.09.012_bib0100","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.exppara.2006.07.002","article-title":"Leishmania major: immune response in BALB\/c mice immunized with stress-inducible protein 1 encapsulated in liposomes","volume":"115","author":"Badiee","year":"2007","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.cimid.2018.09.012_bib0105","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/S1995-7645(14)60087-0","article-title":"Tissue tropism and parasite burden of Toxoplasma gondii RH strain in experimentally infected mice","volume":"7","author":"Dadimoghaddam","year":"2014","journal-title":"Asian Pac. J. Trop. Med."},{"key":"10.1016\/j.cimid.2018.09.012_bib0110","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1007\/s00436-016-5325-6","article-title":"Immunological evaluation of a DNA cocktail vaccine with co-delivery of calcium phosphate nanoparticles (CaPNs) against the Toxoplasma gondii RH strain in BALB\/c mice","volume":"116","author":"Rahimi","year":"2017","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.cimid.2018.09.012_bib0115","doi-asserted-by":"crossref","first-page":"7128","DOI":"10.1128\/AAC.01323-16","article-title":"Evaluation of propranolol effect on experimental acute and chronic toxoplasmosis using quantitative PCR","volume":"60","author":"Montazeri","year":"2016","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.cimid.2018.09.012_bib0120","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0923-2494(05)80096-3","article-title":"Excreted\/secreted antigens of Toxoplasma gondii--their origin and role in the host-parasite interaction","volume":"144","author":"Cesbron-Delauw","year":"1993","journal-title":"Res. Immunol."},{"key":"10.1016\/j.cimid.2018.09.012_bib0125","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S0304-4017(03)00044-X","article-title":"Immune responses against excreted\/secreted antigens of Toxoplasma gondii tachyzoites in the murine model","volume":"113","author":"Daryani","year":"2003","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.cimid.2018.09.012_bib0130","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/nrmicro1800","article-title":"Kiss and spit: the dual roles of Toxoplasma rhoptries","volume":"6","author":"Boothroyd","year":"2008","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.cimid.2018.09.012_bib0135","doi-asserted-by":"crossref","first-page":"381","DOI":"10.3109\/21691401.2014.953633","article-title":"Application of liposomes in medicine and drug delivery","volume":"44","author":"Daraee","year":"2016","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"10.1016\/j.cimid.2018.09.012_bib0140","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1080\/00365540310000102","article-title":"A novel thiocationic liposomal formulation of antisense oligonucleotides with activity against Mycobacterium tuberculosis","volume":"35","author":"Das","year":"2003","journal-title":"Scand. J. Infect. Dis."},{"key":"10.1016\/j.cimid.2018.09.012_bib0145","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1556\/AMicr.57.2010.3.8","article-title":"In vitro activity of clarithromycin in combination with other antimicrobial agents against biofilm-forming Pseudomonas aeruginosa strains","volume":"57","author":"K\u00e1d\u00e1r","year":"2010","journal-title":"Acta Microbiol. Immunol. Hung."},{"key":"10.1016\/j.cimid.2018.09.012_bib0150","doi-asserted-by":"crossref","first-page":"60","DOI":"10.2307\/3281275","article-title":"Live and killed vaccines against toxoplasmosis in mice","author":"Waldeland","year":"1983","journal-title":"J. Parasitol."},{"key":"10.1016\/j.cimid.2018.09.012_bib0155","doi-asserted-by":"crossref","first-page":"2082","DOI":"10.1128\/iai.61.5.2082-2088.1993","article-title":"Oral immunization with Toxoplasma gondii antigens in association with cholera toxin induces enhanced protective and cell-mediated immunity in C57BL\/6 mice","volume":"61","author":"Bourguin","year":"1993","journal-title":"Infect. Immun."},{"key":"10.1016\/j.cimid.2018.09.012_bib0160","doi-asserted-by":"crossref","first-page":"2639","DOI":"10.1128\/iai.61.6.2639-2643.1993","article-title":"Immune responses and resistance to Toxoplasma gondii in mice immunized with antigens of the parasite incorporated into immunostimulating complexes","volume":"61","author":"Lund\u00e9n","year":"1993","journal-title":"Infect. Immun."},{"key":"10.1016\/j.cimid.2018.09.012_bib0165","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1631\/jzus.B0820300","article-title":"Protective effect of DNA-mediated immunization with liposome-encapsulated GRA4 against infection of Toxoplasma gondii","volume":"10","author":"Chen","year":"2009","journal-title":"J. Zhejiang Univ.-Sci. B"},{"key":"10.1016\/j.cimid.2018.09.012_bib0170","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1016\/j.vaccine.2014.01.095","article-title":"Vaccination with profilin encapsulated in oligomannose-coated liposomes induces significant protective immunity against Toxoplasma gondii","volume":"32","author":"Tanaka","year":"2014","journal-title":"Vaccine"}],"container-title":["Comparative Immunology, Microbiology and Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147957118300754?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147957118300754?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T09:39:11Z","timestamp":1693906751000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0147957118300754"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8]]},"references-count":34,"alternative-id":["S0147957118300754"],"URL":"https:\/\/doi.org\/10.1016\/j.cimid.2018.09.012","relation":{},"ISSN":["0147-9571"],"issn-type":[{"value":"0147-9571","type":"print"}],"subject":[],"published":{"date-parts":[[2018,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Quantification of Toxoplasma gondii in the tissues of BALB\/c mice after immunization with nanoliposomal excretory-secretory antigens using Real-Time PCR","name":"articletitle","label":"Article Title"},{"value":"Comparative Immunology, Microbiology and Infectious Diseases","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cimid.2018.09.012","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}