{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:44:58Z","timestamp":1773287098745,"version":"3.50.1"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Comparative Immunology, Microbiology and Infectious Diseases"],"published-print":{"date-parts":[[2023,5]]},"DOI":"10.1016\/j.cimid.2023.101981","type":"journal-article","created":{"date-parts":[[2023,4,7]],"date-time":"2023-04-07T11:14:27Z","timestamp":1680866067000},"page":"101981","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Genotyping of Brucella isolates from animals and humans by Multiple-Locus Variable-number Tandem Repeat Analysis (MLVA)"],"prefix":"10.1016","volume":"96","author":[{"given":"Murat","family":"\u00d6zmen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ediz Ka\u011fan","family":"\u00d6zgen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orbay","family":"Say\u0131","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elif","family":"Karadeniz P\u00fct\u00fcr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Biray","family":"Okumu\u015f","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sibel","family":"\u0130ba Y\u0131lmaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehtap H\u00fclya","family":"Aslan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustafa","family":"Ulucan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Berna","family":"Yanmaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Perihan","family":"\u015eeri\u0307fo\u011flu Ba\u011fatir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nevin","family":"Turut","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u015eifa","family":"Karahan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Buket","family":"Ero\u011flu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y\u0131ld\u0131z \u00d6z","family":"G\u00fclseren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U\u011fur","family":"K\u00fc\u00e7\u00fckayan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c7a\u011fatay","family":"Nuhay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seza","family":"Eski\u0307i\u0307zmi\u0307rli\u0307ler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erdem","family":"Saka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asuman","family":"Soysal Sari\u015fahi\u0307n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0130brahim","family":"Deni\u0307z","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nurg\u00fcl","family":"Bi\u0307rben","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerem","family":"Karaku\u015f","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esra","family":"\u015een","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmet Murat","family":"Sayteki\u0307n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kadir","family":"Akar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.cimid.2023.101981_bib1","unstructured":"WOAH, Anthrax, Terrestrial Manual 2018, pp. 1\u201314."},{"issue":"1","key":"10.1016\/j.cimid.2023.101981_bib2","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1080\/01652176.2020.1868616","article-title":"Bovine brucellosis - a comprehensive review","volume":"41","author":"Khurana","year":"2021","journal-title":"Vet. Q"},{"issue":"12","key":"10.1016\/j.cimid.2023.101981_bib3","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/S1473-3099(07)70286-4","article-title":"Human brucellosis","volume":"7","author":"Franco","year":"2007","journal-title":"Lancet Infect. Dis."},{"issue":"6","key":"10.1016\/j.cimid.2023.101981_bib4","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1016\/j.meegid.2009.07.001","article-title":"Current understanding of the genetic diversity of Brucella, an expanding genus of zoonotic pathogens","volume":"9","author":"Whatmore","year":"2009","journal-title":"Infect., Genet. Evol."},{"issue":"2","key":"10.1016\/j.cimid.2023.101981_bib5","doi-asserted-by":"crossref","first-page":"277","DOI":"10.20506\/rst.21.2.1333","article-title":"Brucellosis in wildlife","volume":"21","author":"Godfroid","year":"2002","journal-title":"Rev. Sci. Tech."},{"key":"10.1016\/j.cimid.2023.101981_bib6","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.meegid.2018.06.024","article-title":"Pathogenicity, population genetics and dissemination of Bacillus anthracis","volume":"64","author":"Pilo","year":"2018","journal-title":"Infect. Genet Evol."},{"key":"10.1016\/j.cimid.2023.101981_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.meegid.2021.104865","article-title":"Emerging diversity and ongoing expansion of the genus Brucella","volume":"92","author":"Whatmore","year":"2021","journal-title":"Infect. Genet Evol."},{"issue":"6","key":"10.1016\/j.cimid.2023.101981_bib8","doi-asserted-by":"crossref","first-page":"1982","DOI":"10.1128\/JCM.02039-05","article-title":"Identification and characterization of variable-number tandem-repeat markers for typing of Brucella spp","volume":"44","author":"Whatmore","year":"2006","journal-title":"J. Clin. Microbiol"},{"issue":"1","key":"10.1016\/j.cimid.2023.101981_bib9","doi-asserted-by":"crossref","first-page":"68","DOI":"10.32707\/ercivet.537995","article-title":"Bruselloz tan\u0131s\u0131 ve epidemiyolojik \u00e7al\u0131\u015fmalarda molek\u00fcler y\u00f6ntemlerin kullan\u0131lmas\u0131","volume":"16","author":"Oktay","year":"2019","journal-title":"Erciyes \u00dcniversitesi Vet. Fak\u00fcltesi Derg."},{"issue":"1","key":"10.1016\/j.cimid.2023.101981_bib10","first-page":"53","article-title":"Laboratory diagnosis of brucellosis in a rural endemic area in northeastern Spain","volume":"7","author":"Serra","year":"2004","journal-title":"Int Microbiol"},{"issue":"3","key":"10.1016\/j.cimid.2023.101981_bib11","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1128\/JCM.02320-16","article-title":"The Bactec FX blood culture system detects brucella melitensis bacteremia in adult patients within the routine 1-week incubation period","volume":"55","author":"Sagi","year":"2017","journal-title":"J. Clin. Microbiol"},{"issue":"4","key":"10.1016\/j.cimid.2023.101981_bib12","doi-asserted-by":"crossref","first-page":"172","DOI":"10.3390\/zoonoticdis2040016","article-title":"Preventing laboratory-acquired brucellosis in the era of MALDI-TOF technology and molecular tests: a narrative review","volume":"2","author":"Yagupsky","year":"2022","journal-title":"Zoonotic Dis."},{"issue":"1","key":"10.1016\/j.cimid.2023.101981_bib13","doi-asserted-by":"crossref","first-page":"13","DOI":"10.56808\/2985-1130.2361","article-title":"Detection and segregation of Brucella abortus and Brucella melitensis in Aborted Bovine, Ovine, Caprine, Buffaloes and Camelid Fetuses by application of conventional and real-time polymerase chain reaction","volume":"42","author":"Dehkordi","year":"2012","journal-title":"Thai J. Vet. Med."},{"issue":"1","key":"10.1016\/j.cimid.2023.101981_bib14","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.mimet.2006.12.015","article-title":"Evaluation of Brucella MLVA typing for human brucellosis","volume":"69","author":"Al Dahouk","year":"2007","journal-title":"J. Microbiol Methods"},{"key":"10.1016\/j.cimid.2023.101981_bib15","unstructured":"S. Aslan, Klinik \u00f6rneklerden izole edilen Brucella izolatlar\u0131n\u0131n epidemiyolojik \u00f6zelliklerinin multi-locus variable number tandem repeat analysis ve pulsed field gel electrophoresis y\u00f6ntemleri ile tespiti, Department of Medical Microbiology, \u00c7ukurova University, Ulusal Tez Merkezi, 2015."},{"issue":"4","key":"10.1016\/j.cimid.2023.101981_bib16","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1016\/j.biochi.2007.07.014","article-title":"On-line resources for bacterial micro-evolution studies using MLVA or CRISPR typing","volume":"90","author":"Grissa","year":"2008","journal-title":"Biochimie"},{"issue":"1","key":"10.1016\/j.cimid.2023.101981_bib17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2180-6-9","article-title":"Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay","volume":"6","author":"Le Fl\u00e8che","year":"2006","journal-title":"BMC Microbiol."},{"issue":"1","key":"10.1016\/j.cimid.2023.101981_bib18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13104-015-1173-1","article-title":"Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR","volume":"8","author":"Wareth","year":"2015","journal-title":"BMC Res. Notes"},{"issue":"3","key":"10.1016\/j.cimid.2023.101981_bib19","doi-asserted-by":"crossref","first-page":"973","DOI":"10.20506\/rst.24.3.1627","article-title":"Abortion due to Brucella abortus in sheep in Nigeria","volume":"24","author":"Ocholi","year":"2005","journal-title":"Rev. Sci. Tech."},{"issue":"3","key":"10.1016\/j.cimid.2023.101981_bib20","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1177\/030098588602300317","article-title":"Morphologic lesions in Brucella abortus infected ovine fetuses","volume":"23","author":"Gorham","year":"1986","journal-title":"Vet. Pathol."},{"issue":"3","key":"10.1016\/j.cimid.2023.101981_bib21","first-page":"653","article-title":"Isolation and biotyping of Brucella melitensis from Upper Egypt","volume":"7","author":"Affi","year":"2011","journal-title":"J. Am. Sci."},{"issue":"1","key":"10.1016\/j.cimid.2023.101981_bib22","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s12917-022-03155-1","article-title":"Identification and molecular characterization of Brucella abortus and Brucella melitensis isolated from milk in cattle in Azerbaijan","volume":"18","author":"Aliyev","year":"2022","journal-title":"BMC Vet. Res"},{"issue":"1","key":"10.1016\/j.cimid.2023.101981_bib23","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s11250-011-9899-9","article-title":"Infection of cattle in Kenya with Brucella abortus biovar 3 and Brucella melitensis biovar 1 genotypes","volume":"44","author":"Muendo","year":"2012","journal-title":"Trop. Anim. Health Prod."},{"issue":"4","key":"10.1016\/j.cimid.2023.101981_bib24","first-page":"185","article-title":"Isolation and identification of Brucella organisms in Iran, Archives of Clinical Infectious Diseases","volume":"3","author":"Zowghi","year":"2008"},{"issue":"3","key":"10.1016\/j.cimid.2023.101981_bib25","article-title":"The first isolation of Brucella melitensis from bovine aborted fetus in T\u00fcrkiye","volume":"1","author":"Buyukcangaz","year":"2007","journal-title":"J. Biol. Environ. Sci."},{"issue":"1","key":"10.1016\/j.cimid.2023.101981_bib26","doi-asserted-by":"crossref","first-page":"15","DOI":"10.17305\/bjbms.2006.3203","article-title":"Brucella melitensis: review of the human infection case","volume":"6","author":"Zvizdi\u0107","year":"2006","journal-title":"Bosn. J. Basic Med Sci."},{"issue":"4","key":"10.1016\/j.cimid.2023.101981_bib27","first-page":"315","article-title":"Molecular identification of Brucella species and biovars associated with animal and human infection in Iran","volume":"10","author":"Dadar","year":"2019","journal-title":"Vet. Res Forum"},{"key":"10.1016\/j.cimid.2023.101981_bib28","doi-asserted-by":"crossref","DOI":"10.1155\/2014\/596245","article-title":"Identification and in vitro antimicrobial susceptibility of Brucella species isolated from human brucellosis","volume":"2014","author":"Hashim","year":"2014","journal-title":"Int J. Microbiol"},{"issue":"3","key":"10.1016\/j.cimid.2023.101981_bib29","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1080\/00365540600978898","article-title":"Asymptomatic Brucella bacteraemia and isolation of Brucella melitensis biovar 3 from human breast milk","volume":"39","author":"Celebi","year":"2007","journal-title":"Scand. J. Infect. Dis."},{"issue":"4","key":"10.1016\/j.cimid.2023.101981_bib30","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1002\/jobm.200410469","article-title":"Identification of Brucella species isolated from proven brucellosis patients in Izmir, T\u00fcrkiye","volume":"45","author":"K\u00f6se","year":"2005","journal-title":"J. Basic Microbiol"},{"issue":"9","key":"10.1016\/j.cimid.2023.101981_bib31","doi-asserted-by":"crossref","first-page":"3276","DOI":"10.1128\/JCM.02538-10","article-title":"Multiple-locus variable-number tandem-repeat analysis genotyping of human Brucella isolates from T\u00fcrkiye","volume":"49","author":"K\u0131l\u0131\u00e7","year":"2011","journal-title":"J. Clin. Microbiol."},{"issue":"3","key":"10.1016\/j.cimid.2023.101981_bib32","article-title":"Molecular investigation of the transmission dynamics of brucellosis observed among children in the province of South-East Anatolia, T\u00fcrkiye, Jundishapur","volume":"11","author":"Dal","year":"2018","journal-title":"J. Microbiol."},{"issue":"10","key":"10.1016\/j.cimid.2023.101981_bib33","article-title":"MLVA genotyping of Brucella melitensis and Brucella abortus isolates from different animal species and humans and identification of Brucella suis vaccine strain S2 from cattle in China","volume":"8","author":"Jiang","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.cimid.2023.101981_bib34","first-page":"1","article-title":"MLVA typing of Brucella melitensis and B. abortus isolates of animal and human origin from India","author":"Kumari","year":"2021","journal-title":"Anim. Biotechnol."},{"key":"10.1016\/j.cimid.2023.101981_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.bjid.2020.11.008","article-title":"Determination of investigation of the link between human and animal Brucella isolates in Iran using multiple-locus variable number tandem repeat method comprising 16 loci (MLVA-16)","volume":"25","author":"Mirkalantari","year":"2021","journal-title":"Braz. J. Infect. Dis."},{"issue":"12","key":"10.1016\/j.cimid.2023.101981_bib36","doi-asserted-by":"crossref","first-page":"3935","DOI":"10.1128\/JCM.00464-08","article-title":"Evaluation of a multilocus variable-number tandem-repeat analysis scheme for typing human Brucella isolates in a region of brucellosis endemicity","volume":"46","author":"Kattar","year":"2008","journal-title":"J. Clin. Microbiol."},{"issue":"8","key":"10.1016\/j.cimid.2023.101981_bib37","doi-asserted-by":"crossref","first-page":"2734","DOI":"10.1128\/JCM.00533-10","article-title":"Epidemiological and phylogenetic analysis of Spanish human Brucella melitensis strains by multiple-locus variable-number tandem-repeat typing, hypervariable octameric oligonucleotide fingerprinting, and rpoB typing","volume":"48","author":"Valdezate","year":"2010","journal-title":"J. Clin. Microbiol"},{"issue":"6\u20137","key":"10.1016\/j.cimid.2023.101981_bib38","doi-asserted-by":"crossref","first-page":"536","DOI":"10.3109\/00365548.2011.562531","article-title":"Imported brucellosis in Denmark: molecular identification and multiple-locus variable number tandem repeat analysis (MLVA) genotyping of the bacteria","volume":"43","author":"Aftab","year":"2011","journal-title":"Scand. J. Infect. Dis."},{"key":"10.1016\/j.cimid.2023.101981_bib39","doi-asserted-by":"crossref","unstructured":"A.C. Ferreira, L. Chambel, T. Tenreiro, R. Cardoso, L. Flor, I.T. Dias, T. Pacheco, B. Garin-Bastuji, P. Le Fleche, G. Vergnaud, MLVA16 typing of Portuguese human and animal Brucella melitensis and Brucella abortus isolates, (2012).","DOI":"10.1371\/journal.pone.0042514"},{"key":"10.1016\/j.cimid.2023.101981_bib40","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1186\/1471-2180-11-256","article-title":"MLVA genotyping of Chinese human Brucella melitensis biovar 1, 2 and 3 isolates","volume":"11","author":"Jiang","year":"2011","journal-title":"BMC Microbiol"},{"issue":"6","key":"10.1016\/j.cimid.2023.101981_bib41","doi-asserted-by":"crossref","first-page":"498","DOI":"10.3390\/pathogens9060498","article-title":"MLVA-16 genotyping of Brucella abortus and Brucella melitensis isolates from different animal species in Egypt: Geographical relatedness and the mediterranean lineage","volume":"9","author":"Wareth","year":"2020","journal-title":"Pathogens"}],"container-title":["Comparative Immunology, Microbiology and Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147957123000395?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0147957123000395?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T06:48:26Z","timestamp":1758955706000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0147957123000395"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5]]},"references-count":41,"alternative-id":["S0147957123000395"],"URL":"https:\/\/doi.org\/10.1016\/j.cimid.2023.101981","relation":{},"ISSN":["0147-9571"],"issn-type":[{"value":"0147-9571","type":"print"}],"subject":[],"published":{"date-parts":[[2023,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Genotyping of Brucella isolates from animals and humans by Multiple-Locus Variable-number Tandem Repeat Analysis (MLVA)","name":"articletitle","label":"Article Title"},{"value":"Comparative Immunology, Microbiology and Infectious Diseases","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cimid.2023.101981","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"101981"}}