{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T04:05:14Z","timestamp":1793160314766,"version":"4.5.0"},"reference-count":33,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T00:00:00Z","timestamp":1739923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","contactlensjournal.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Contact Lens and Anterior Eye"],"published-print":{"date-parts":[[2025,7]]},"DOI":"10.1016\/j.clae.2025.102388","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T16:29:04Z","timestamp":1742228944000},"page":"102388","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":19,"title":["Global practices of tear fluid collection, storage, and molecular analysis \u2013 A questionnaire by the Tear Research Network"],"prefix":"10.1016","volume":"48","author":[{"given":"Nikolay","family":"Boychev","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swaminathan","family":"Sethu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romy op","family":"de Laak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Brignole-Baudouin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nienke","family":"van de Sande","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine S","family":"Held","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amalia Enr\u00edquez","family":"de Salamanca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne","family":"Hagan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9mence","family":"Bonnet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Yeung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menglu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arkasubhra","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7203-0159","authenticated-orcid":false,"given":"Marlies","family":"Gijs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.clae.2025.102388_b0005","doi-asserted-by":"crossref","first-page":"10064","DOI":"10.1038\/s41598-021-89514-8","article-title":"Diagnostic biomarkers in tear fluid: from sampling to preanalytical processing","volume":"11","author":"Bachhuber","year":"2021","journal-title":"Sci Rep"},{"issue":"8","key":"10.1016\/j.clae.2025.102388_b0010","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1080\/14737159.2021.1941879","article-title":"Human tear fluid analysis for clinical applications: progress and prospects","volume":"21","author":"Adigal","year":"2021","journal-title":"Expert Rev Mol Diagn"},{"issue":"6","key":"10.1016\/j.clae.2025.102388_b0015","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.preteyeres.2012.06.002","article-title":"Tear analysis in ocular surface diseases","volume":"31","author":"Zhou","year":"2012","journal-title":"Prog Retin Eye Res"},{"issue":"22","key":"10.1016\/j.clae.2025.102388_b0020","doi-asserted-by":"crossref","first-page":"5388","DOI":"10.3390\/jcm10225388","article-title":"Protein biomarkers in glaucoma: a review","volume":"10","author":"Fiedorowicz","year":"2021","journal-title":"J Clin Med"},{"issue":"10","key":"10.1016\/j.clae.2025.102388_b0025","doi-asserted-by":"crossref","first-page":"1354","DOI":"10.1002\/bmc.2996","article-title":"Utility of noninvasive biomatrices in pharmacokinetic studies","volume":"27","author":"Raju","year":"2013","journal-title":"Biomed Chromatogr"},{"key":"10.1016\/j.clae.2025.102388_b0030","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13167-016-0065-3","article-title":"Tear fluid biomarkers in ocular and systemic disease: potential use for predictive, preventive and personalised medicine","volume":"7","author":"Hagan","year":"2016","journal-title":"Epma Journal"},{"key":"10.1016\/j.clae.2025.102388_b0035","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.exer.2013.07.015","article-title":"Tears as a source of biomarkers for ocular and systemic diseases","volume":"117","author":"Hohenstein-Blaul","year":"2013","journal-title":"Exp Eye Res"},{"issue":"1","key":"10.1016\/j.clae.2025.102388_b0040","doi-asserted-by":"crossref","first-page":"22675","DOI":"10.1038\/s41598-021-01993-x","article-title":"Association of tear fluid amyloid and tau levels with disease severity and neurodegeneration","volume":"11","author":"Gijs","year":"2021","journal-title":"Sci Rep"},{"issue":"1","key":"10.1016\/j.clae.2025.102388_b0045","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1186\/s12883-023-03335-y","article-title":"Tear biomarkers for Alzheimer\u2019s disease screening and diagnosis (the TearAD study): design and rationale of an observational longitudinal multicenter study","volume":"23","author":"van de Sande","year":"2023","journal-title":"BMC Neurol"},{"issue":"1","key":"10.1016\/j.clae.2025.102388_b0050","doi-asserted-by":"crossref","first-page":"15437","DOI":"10.1038\/s41598-019-51837-y","article-title":"Proteins and microRNAs are differentially expressed in tear fluid from patients with Alzheimer\u2019s disease","volume":"9","author":"Kenny","year":"2019","journal-title":"Sci Rep"},{"key":"10.1016\/j.clae.2025.102388_b0055","doi-asserted-by":"crossref","DOI":"10.1177\/13872877241295315","article-title":"Tear fluid reflects the altered protein expressions of Alzheimer's disease patients in proteins involved in protein repair and clearance system or the regulation of cytoskeleton","author":"K\u00e4rkk\u00e4inen","year":"2024","journal-title":"Journal of Alzheimer\u2019s Disease"},{"issue":"3","key":"10.1016\/j.clae.2025.102388_b0060","doi-asserted-by":"crossref","first-page":"2590","DOI":"10.3390\/ijms24032590","article-title":"Beta-amyloid peptide in tears: an early diagnostic marker of Alzheimer\u2019s disease correlated with choroidal thickness","volume":"24","author":"Gharbiya","year":"2023","journal-title":"Int J Mol Sci"},{"issue":"11","key":"10.1016\/j.clae.2025.102388_b0065","doi-asserted-by":"crossref","first-page":"941","DOI":"10.2217\/bmm-2019-0167","article-title":"Oligomeric \u03b1-synuclein is increased in basal tears of Parkinson\u2019s patients","volume":"13","author":"Hamm-Alvarez","year":"2019","journal-title":"Biomark Med"},{"issue":"1","key":"10.1016\/j.clae.2025.102388_b0070","doi-asserted-by":"crossref","first-page":"8507","DOI":"10.1038\/s41598-020-65503-1","article-title":"Increased alpha-synuclein tear fluid levels in patients with Parkinson\u2019s disease","volume":"10","author":"Maass","year":"2020","journal-title":"Sci Rep"},{"issue":"3","key":"10.1016\/j.clae.2025.102388_b0075","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1007\/s11357-022-00576-6","article-title":"Elevated \u03b1-synuclein and NfL levels in tear fluids and decreased retinal microvascular densities in patients with Parkinson\u2019s disease","volume":"44","author":"Lin","year":"2022","journal-title":"Geroscience"},{"issue":"1","key":"10.1016\/j.clae.2025.102388_b0080","doi-asserted-by":"crossref","first-page":"4","DOI":"10.3390\/proteomes10010004","article-title":"Potential tear biomarkers for the diagnosis of Parkinson\u2019s disease\u2014a pilot study","volume":"10","author":"Acera","year":"2022","journal-title":"Proteomes"},{"issue":"2","key":"10.1016\/j.clae.2025.102388_b0085","doi-asserted-by":"crossref","first-page":"181","DOI":"10.14802\/jmd.24014","article-title":"High levels of mutant huntingtin protein in tear fluid from Huntington\u2019s disease gene expansion carriers","volume":"17","author":"Gijs","year":"2024","journal-title":"J Movement Disorders"},{"issue":"3","key":"10.1016\/j.clae.2025.102388_b0090","article-title":"Toward a label-free electrochemical impedance immunosensor design for quantifying cortisol in tears. Critical Reviews\u2122","volume":"47","author":"Cardinell","year":"2019","journal-title":"Biomed Eng"},{"issue":"4","key":"10.1016\/j.clae.2025.102388_b0095","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1038\/s41587-019-0045-y","article-title":"Wearable biosensors for healthcare monitoring","volume":"37","author":"Kim","year":"2019","journal-title":"Nat Biotechnol"},{"issue":"10","key":"10.1016\/j.clae.2025.102388_b0100","doi-asserted-by":"crossref","first-page":"483","DOI":"10.3390\/bios14100483","article-title":"Tear-based ocular wearable biosensors for human health monitoring","volume":"14","author":"Rajan","year":"2024","journal-title":"Biosensors"},{"issue":"10","key":"10.1016\/j.clae.2025.102388_b0105","doi-asserted-by":"crossref","first-page":"1731","DOI":"10.1038\/s41433-020-0930-0","article-title":"Tear analysis as the next routine body fluid test","volume":"34","author":"Barmada","year":"2020","journal-title":"Eye"},{"issue":"3","key":"10.1016\/j.clae.2025.102388_b0110","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1007\/s11095-019-2569-8","article-title":"Review of biomarkers in ocular matrices: challenges and opportunities","volume":"36","author":"Tamhane","year":"2019","journal-title":"Pharm Res"},{"issue":"6","key":"10.1016\/j.clae.2025.102388_b0115","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1097\/00003226-199413060-00007","article-title":"Rapid assay of lactoferrin in keratoconjunctivitis sicca","volume":"13","author":"McCollum","year":"1994","journal-title":"Cornea"},{"key":"10.1016\/j.clae.2025.102388_b0120","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.exer.2013.07.019","article-title":"Allergy and allergic mediators in tears","volume":"117","author":"Leonardi","year":"2013","journal-title":"Exp Eye Res"},{"issue":"8","key":"10.1016\/j.clae.2025.102388_b0125","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1097\/ICO.0b013e3182532047","article-title":"Evaluation of tear osmolarity in non-Sj\u00f6gren and Sj\u00f6gren syndrome dry eye patients with the TearLab system","volume":"31","author":"Szalai","year":"2012","journal-title":"Cornea"},{"issue":"6","key":"10.1016\/j.clae.2025.102388_b0130","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1159\/000150445","article-title":"Inflammatory markers in the tears of patients with ocular surface disease","volume":"40","author":"Acera","year":"2008","journal-title":"Ophthalmic Res"},{"issue":"3","key":"10.1016\/j.clae.2025.102388_b0135","first-page":"264","article-title":"Determination of tear volume and tear flow","volume":"5","author":"Mishima","year":"1966","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"1","key":"10.1016\/j.clae.2025.102388_b0140","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/0014-4835(84)90134-9","article-title":"Diurnal variation in human tear enzymes","volume":"38","author":"Fullard","year":"1984","journal-title":"Exp Eye Res"},{"issue":"6","key":"10.1016\/j.clae.2025.102388_b0145","article-title":"Diurnal variation on tear stability and correlation with tear cytokine concentration","volume":"45","author":"Arroyo-del Arroyo","year":"2022","journal-title":"Cont Lens Anterior Eye"},{"issue":"5","key":"10.1016\/j.clae.2025.102388_b0150","doi-asserted-by":"crossref","first-page":"2245","DOI":"10.1177\/1120672121998922","article-title":"Tear fluid collection methods: Review of current techniques","volume":"31","author":"Pieczy\u0144ski","year":"2021","journal-title":"Eur J Ophthalmol"},{"issue":"1","key":"10.1016\/j.clae.2025.102388_b0155","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1038\/s41598-023-28363-z","article-title":"Pre-analytical sample handling effects on tear fluid protein levels","volume":"13","author":"Gijs","year":"2023","journal-title":"Sci Rep"},{"issue":"21","key":"10.1016\/j.clae.2025.102388_b0160","doi-asserted-by":"crossref","first-page":"5088","DOI":"10.1039\/c2an35821b","article-title":"Post-collection processing of Schirmer strip-collected human tear fluid impacts protein content","volume":"137","author":"Denisin","year":"2012","journal-title":"Analyst"},{"key":"10.1016\/j.clae.2025.102388_b0165","doi-asserted-by":"crossref","unstructured":"Gijs M. A comprehensive review of methodological approaches and clinical applications of tear fluid biomarkers. Prog Retin Eye Res 2025. Feb 13;106:101338.","DOI":"10.1016\/j.preteyeres.2025.101338"}],"container-title":["Contact Lens and Anterior Eye"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1367048425000220?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1367048425000220?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T15:57:44Z","timestamp":1749571064000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1367048425000220"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":33,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["S1367048425000220"],"URL":"https:\/\/doi.org\/10.1016\/j.clae.2025.102388","relation":{},"ISSN":["1367-0484"],"issn-type":[{"value":"1367-0484","type":"print"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Global practices of tear fluid collection, storage, and molecular analysis \u2013 A questionnaire by the Tear Research Network","name":"articletitle","label":"Article Title"},{"value":"Contact Lens and Anterior Eye","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.clae.2025.102388","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Ltd on behalf of British Contact Lens Association.","name":"copyright","label":"Copyright"}],"article-number":"102388"}}