{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:15:58Z","timestamp":1791400558053,"version":"4.3.3"},"reference-count":57,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T00:00:00Z","timestamp":1739836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001279","name":"Great Ormond Street Hospital Children's Charity","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001279","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["222096\/Z\/20\/Z"],"award-info":[{"award-number":["222096\/Z\/20\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["217112\/Z\/19\/Z"],"award-info":[{"award-number":["217112\/Z\/19\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["104807\/Z\/14\/Z"],"award-info":[{"award-number":["104807\/Z\/14\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["320030_205097"],"award-info":[{"award-number":["320030_205097"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"UK Research and Innovation Medical Research Council","doi-asserted-by":"publisher","award":["MR\/Y013395\/1"],"award-info":[{"award-number":["MR\/Y013395\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014013","name":"UK Research and Innovation","doi-asserted-by":"publisher","award":["MR\/L012758\/1"],"award-info":[{"award-number":["MR\/L012758\/1"]}],"id":[{"id":"10.13039\/100014013","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006447","name":"Universit\u00e4t Z\u00fcrich","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006447","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009553","name":"Agentura Pro Zdravotnick\u00fd V\u00fdzkum \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009553","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009647","name":"Ministry of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009647","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000272","name":"National Institute for Health and Care Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Clinical Immunology"],"published-print":{"date-parts":[[2025,5]]},"DOI":"10.1016\/j.clim.2025.110453","type":"journal-article","created":{"date-parts":[[2025,2,16]],"date-time":"2025-02-16T18:19:26Z","timestamp":1739729966000},"page":"110453","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Ex vivo T-lymphopoiesis assays assisting corrective treatment choice for genetically undefined T-lymphocytopenia"],"prefix":"10.1016","volume":"274","author":[{"given":"Zainab M.","family":"Golwala","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Spiridou Goncalves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranjita Devi","family":"Moirangthem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace","family":"Evans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina","family":"Lizot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coco","family":"de Koning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandrine","family":"Garrigue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta Martin","family":"Corredera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Moises","family":"Ocampo-Godinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evey","family":"Howley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Kricke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnold","family":"Awuah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Obiri-Yeboa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajeev","family":"Rai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"Sebire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanette","family":"Bernard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Bordon Cueto De Braem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaan","family":"Boztug","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa","family":"Cole","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew R.","family":"Gennery","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott","family":"Hackett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Hambleton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mette","family":"Holm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maaike A.","family":"Kusters","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Klocperk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Marzollo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nufar","family":"Marcus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zohreh","family":"Nademi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Pachlopnik Schmid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert","family":"Pichler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Sellmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pere","family":"Soler-Palacin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maarja","family":"Soomann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Torpiano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joris","family":"van Montfrans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Nierkens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart","family":"Adams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Buckland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly","family":"Gilmour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Austen","family":"Worth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian J.","family":"Thrasher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. Graham","family":"Davies","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Andr\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra Y.","family":"Kreins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.clim.2025.110453_bb0005","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.jaci.2022.10.022","article-title":"The diagnosis of severe combined immunodeficiency (SCID): the primary immune deficiency treatment consortium (PIDTC) 2022 definitions","volume":"151","author":"Dvorak","year":"2023","journal-title":"J. Allergy Clin. Immunol."},{"issue":"7","key":"10.1016\/j.clim.2025.110453_bb0010","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1007\/s10875-022-01289-3","article-title":"Human inborn errors of immunity: 2022 update on the classification from the International Union of Immunological Societies Expert Committee","volume":"42","author":"Tangye","year":"2022","journal-title":"J. Clin. Immunol."},{"issue":"5","key":"10.1016\/j.clim.2025.110453_bb0015","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.1016\/j.jaci.2021.10.017","article-title":"Hematopoietic cell transplantation in severe combined immunodeficiency: the SCETIDE 2006-2014 European cohort","volume":"149","author":"Lankester","year":"2022","journal-title":"J. Allergy Clin. Immunol."},{"issue":"10396","key":"10.1016\/j.clim.2025.110453_bb0020","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0140-6736(23)00731-6","article-title":"Measuring the effect of newborn screening on survival after haematopoietic cell transplantation for severe combined immunodeficiency: a 36-year longitudinal study from the primary immune deficiency treatment consortium","volume":"402","author":"Thakar","year":"2023","journal-title":"Lancet"},{"key":"10.1016\/j.clim.2025.110453_bb0025","first-page":"349","article-title":"Pegademase bovine (PEG-ADA) for the treatment of infants and children with severe combined immunodeficiency (SCID)","volume":"3","author":"Booth","year":"2009","journal-title":"Biologics"},{"issue":"1","key":"10.1016\/j.clim.2025.110453_bb0030","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1097\/ACI.0000000000000876","article-title":"Gene therapy for inborn error of immunity - current status and future perspectives","volume":"23","author":"Mudde","year":"2023","journal-title":"Curr. Opin. Allergy Clin. Immunol."},{"key":"10.1016\/j.clim.2025.110453_bb0035","doi-asserted-by":"crossref","first-page":"373","DOI":"10.3389\/fped.2019.00373","article-title":"Universal newborn screening for severe combined immunodeficiency (SCID)","volume":"7","author":"van der Burg","year":"2019","journal-title":"Front. Pediatr."},{"issue":"1","key":"10.1016\/j.clim.2025.110453_bb0040","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1111\/imr.12729","article-title":"Newborn screening for severe combined immunodeficiency and T-cell lymphopenia","volume":"287","author":"Puck","year":"2019","journal-title":"Immunol. Rev."},{"issue":"2","key":"10.1016\/j.clim.2025.110453_bb0045","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.jaci.2024.10.018","article-title":"Newborn screening for SCID and severe T lymphocytopenia in Europe","volume":"155","author":"Blom","year":"2024","journal-title":"J. Allergy Clin. Immunol."},{"issue":"1","key":"10.1016\/j.clim.2025.110453_bb0050","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/s10875-023-01640-2","article-title":"Reducing mortality and morbidity in children with severe combined immunodeficiency in Switzerland: the role of newborn screening","volume":"44","author":"Soomann","year":"2024","journal-title":"J. Clin. Immunol."},{"issue":"1","key":"10.1016\/j.clim.2025.110453_bb0055","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.jaci.2023.08.031","article-title":"Impact of newborn screening for SCID on the management of congenital athymia","volume":"153","author":"Howley","year":"2024","journal-title":"J. Allergy Clin. Immunol."},{"issue":"5","key":"10.1016\/j.clim.2025.110453_bb0060","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1007\/s10875-023-01450-6","article-title":"Prospective newborn screening for SCID in Germany: a first analysis by the pediatric immunology working group (API)","volume":"43","author":"Speckmann","year":"2023","journal-title":"J. Clin. Immunol."},{"issue":"1","key":"10.1016\/j.clim.2025.110453_bb0065","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/s00281-020-00826-9","article-title":"Inborn errors of thymic stromal cell development and function","volume":"43","author":"Kreins","year":"2021","journal-title":"Semin. Immunopathol."},{"key":"10.1016\/j.clim.2025.110453_bb0070","doi-asserted-by":"crossref","DOI":"10.1016\/j.clim.2023.109757","article-title":"Expanding the clinical and immunological phenotypes of PAX1-deficient SCID and CID patients","volume":"255","author":"Yakici","year":"2023","journal-title":"Clin. Immunol."},{"issue":"4","key":"10.1016\/j.clim.2025.110453_bb0075","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1007\/s10875-021-00967-y","article-title":"Expanding the nude SCID\/CID phenotype associated with FOXN1 homozygous, compound heterozygous, or heterozygous mutations","volume":"41","author":"Giardino","year":"2021","journal-title":"J. Clin. Immunol."},{"issue":"2","key":"10.1016\/j.clim.2025.110453_bb0080","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/j.jaci.2021.06.028","article-title":"Experience with cultured thymus tissue in 105 children","volume":"149","author":"Markert","year":"2022","journal-title":"J. Allergy Clin. Immunol."},{"issue":"6","key":"10.1016\/j.clim.2025.110453_bb0085","doi-asserted-by":"crossref","first-page":"1660","DOI":"10.1016\/j.jaci.2017.03.020","article-title":"Thymus transplantation for complete DiGeorge syndrome: European experience","volume":"140","author":"Davies","year":"2017","journal-title":"J. Allergy Clin. Immunol."},{"issue":"6","key":"10.1016\/j.clim.2025.110453_bb0090","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1007\/s10875-023-01478-8","article-title":"First use of Thymus transplantation in PAX1 deficiency","volume":"43","author":"Kreins","year":"2023","journal-title":"J. Clin. Immunol."},{"issue":"6","key":"10.1016\/j.clim.2025.110453_bb0095","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1016\/j.jaci.2024.07.031","article-title":"European Society for Immunodeficiencies guidelines for the management of patients with congenital athymia","volume":"154","author":"Kreins","year":"2024","journal-title":"J. Allergy Clin. Immunol."},{"issue":"3","key":"10.1016\/j.clim.2025.110453_bb0100","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1007\/s10875-022-01403-5","article-title":"Impact of genetic diagnosis on the outcome of hematopoietic stem cell transplant in primary immunodeficiency disorders","volume":"43","author":"Forlanini","year":"2023","journal-title":"J. Clin. Immunol."},{"issue":"3","key":"10.1016\/j.clim.2025.110453_bb0105","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/s10875-024-01678-w","article-title":"Parental engagement in identifying information needs after newborn screening for families of infants with suspected Athymia","volume":"44","author":"Howley","year":"2024","journal-title":"J. Clin. Immunol."},{"issue":"2","key":"10.1016\/j.clim.2025.110453_bb0110","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1016\/j.jaci.2020.05.046","article-title":"Improving the diagnostic efficiency of primary immunodeficiencies with targeted next-generation sequencing","volume":"147","author":"Fusaro","year":"2021","journal-title":"J. Allergy Clin. Immunol."},{"issue":"4","key":"10.1016\/j.clim.2025.110453_bb0115","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1016\/j.jaci.2016.08.003","article-title":"Exome and genome sequencing for inborn errors of immunity","volume":"138","author":"Meyts","year":"2016","journal-title":"J. Allergy Clin. Immunol."},{"issue":"1","key":"10.1016\/j.clim.2025.110453_bb0120","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.jaci.2018.08.027","article-title":"The genetic landscape of severe combined immunodeficiency in the United States and Canada in the current era (2010-2018)","volume":"143","author":"Dvorak","year":"2019","journal-title":"J. Allergy Clin. Immunol."},{"key":"10.1016\/j.clim.2025.110453_bb0125","doi-asserted-by":"crossref","first-page":"239","DOI":"10.2147\/TCRM.S379673","article-title":"Congenital Athymia: unmet needs and practical guidance","volume":"19","author":"Howley","year":"2023","journal-title":"Ther. Clin. Risk Manag."},{"issue":"6","key":"10.1016\/j.clim.2025.110453_bb0130","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1097\/ACI.0000000000000695","article-title":"Replacing defective thymus function","volume":"20","author":"Kreins","year":"2020","journal-title":"Curr. Opin. Allergy Clin. Immunol."},{"key":"10.1016\/j.clim.2025.110453_bb0135","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.655354","article-title":"Current and future therapeutic approaches for Thymic stromal cell defects","volume":"12","author":"Kreins","year":"2021","journal-title":"Front. Immunol."},{"issue":"12","key":"10.1016\/j.clim.2025.110453_bb0140","doi-asserted-by":"crossref","first-page":"2611","DOI":"10.1182\/bloodadvances.2020001730","article-title":"Artificial thymic organoids represent a reliable tool to study T-cell differentiation in patients with severe T-cell lymphopenia","volume":"4","author":"Bosticardo","year":"2020","journal-title":"Blood Adv."},{"issue":"12","key":"10.1016\/j.clim.2025.110453_bb0145","doi-asserted-by":"crossref","first-page":"2606","DOI":"10.1182\/bloodadvances.2020001736","article-title":"Diagnostic assay to assist clinical decisions for unclassified severe combined immune deficiency","volume":"4","author":"Bifsha","year":"2020","journal-title":"Blood Adv."},{"issue":"3","key":"10.1016\/j.clim.2025.110453_bb0155","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1089\/scd.1.1996.5.213","article-title":"The ISHAGE guidelines for CD34+ cell determination by flow cytometry. International Society of Hematotherapy and Graft Engineering","volume":"5","author":"Sutherland","year":"1996","journal-title":"J. Hematother."},{"key":"10.1016\/j.clim.2025.110453_bb0160","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.bcmd.2011.04.001","article-title":"Cytokines and culture medium have a major impact on human in vitro T-cell differentiation","volume":"47","author":"Emmanuelle","year":"2011","journal-title":"Blood Cell Mol. Dis."},{"issue":"5","key":"10.1016\/j.clim.2025.110453_bb0165","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1038\/nmeth.4237","article-title":"Generation of mature T cells from human hematopoietic stem and progenitor cells in artificial thymic organoids","volume":"14","author":"Seet","year":"2017","journal-title":"Nat. Methods"},{"issue":"2","key":"10.1016\/j.clim.2025.110453_bb0170","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1111\/cei.13062","article-title":"A comparison of TRECs and flow cytometry for naive T cell quantification","volume":"191","author":"Adams","year":"2018","journal-title":"Clin. Exp. Immunol."},{"issue":"3","key":"10.1016\/j.clim.2025.110453_bb0175","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.jmoldx.2018.02.003","article-title":"Chimerism analysis in the pediatric setting: direct PCR from bone marrow, whole blood, and cell fractions","volume":"20","author":"Kricke","year":"2018","journal-title":"J. Mol. Diagn."},{"issue":"6480","key":"10.1016\/j.clim.2025.110453_bb0180","doi-asserted-by":"crossref","DOI":"10.1126\/science.aay3224","article-title":"A cell atlas of human thymic development defines T cell repertoire formation","volume":"367","author":"Park","year":"2020","journal-title":"Science"},{"issue":"10","key":"10.1016\/j.clim.2025.110453_bb0185","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1038\/s41590-020-0747-9","article-title":"Distinct and temporary-restricted epigenetic mechanisms regulate human alphabeta and gammadelta T cell development","volume":"21","author":"Roels","year":"2020","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.clim.2025.110453_bb0190","series-title":"A spatial human thymus cell atlas mapped to a continuous tissue axis. bioRxiv","author":"Yayon","year":"2023"},{"issue":"1","key":"10.1016\/j.clim.2025.110453_bb0195","doi-asserted-by":"crossref","DOI":"10.1084\/jem.20220979","article-title":"Dominant negative variants in ITPR3 impair T cell Ca2+ dynamics causing combined immunodeficiency","volume":"222","author":"Blanco","year":"2025","journal-title":"J. Exp. Med."},{"issue":"1","key":"10.1016\/j.clim.2025.110453_bb0200","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.jaci.2019.09.020","article-title":"Recurrent microdeletions at chromosome 2p11.2 are associated with thymic hypoplasia and features resembling DiGeorge syndrome","volume":"145","author":"Bernstock","year":"2020","journal-title":"J. Allergy Clin. Immunol."},{"key":"10.1016\/j.clim.2025.110453_bb0205","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1002\/ajmg.1320470729","article-title":"DiGeorge anomaly with renal agenesis in infants of mothers with diabetes","volume":"47","author":"Wilson","year":"1993","journal-title":"Am. J. Med. Genet."},{"key":"10.1016\/j.clim.2025.110453_bb0210","article-title":"Maternal diabetes causing DiGeorge anomaly and renal agenesis","volume":"55","author":"Digilo","year":"1995","journal-title":"Am. J. Med. Genet."},{"issue":"1","key":"10.1016\/j.clim.2025.110453_bb0215","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1017\/S1047951112000194","article-title":"Association of DiGeorge anomaly and caudal dysplasia sequence in a neonate born to a diabetic mother","volume":"23","author":"Dentici","year":"2013","journal-title":"Cardiol. Young"},{"issue":"1","key":"10.1016\/j.clim.2025.110453_bb0220","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1186\/s13073-023-01240-0","article-title":"Structural and non-coding variants increase the diagnostic yield of clinical whole genome sequencing for rare diseases","volume":"15","author":"Pagnamenta","year":"2023","journal-title":"Genome Med."},{"issue":"2","key":"10.1016\/j.clim.2025.110453_bb0225","doi-asserted-by":"crossref","DOI":"10.1542\/peds.2018-2300","article-title":"Newborn screening for severe combined immunodeficiency and T-cell lymphopenia in California, 2010-2017","volume":"143","author":"Amatuni","year":"2019","journal-title":"Pediatrics"},{"issue":"44","key":"10.1016\/j.clim.2025.110453_bb0230","doi-asserted-by":"crossref","DOI":"10.1126\/sciimmunol.aax1036","article-title":"PAX1 is essential for development and function of the human thymus","volume":"5","author":"Yamazaki","year":"2020","journal-title":"Sci. Immunol."},{"issue":"2","key":"10.1016\/j.clim.2025.110453_bb0235","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1007\/s10875-019-00615-6","article-title":"A novel FOXN1 variant is identified in two siblings with nude severe combined immunodeficiency","volume":"39","author":"Firtina","year":"2019","journal-title":"J. Clin. Immunol."},{"issue":"13","key":"10.1016\/j.clim.2025.110453_bb0240","doi-asserted-by":"crossref","first-page":"2229","DOI":"10.1182\/blood-2010-03-275966","article-title":"Multicenter survey on the outcome of transplantation of hematopoietic cells in patients with the complete form of DiGeorge anomaly","volume":"116","author":"Janda","year":"2010","journal-title":"Blood"},{"key":"10.1016\/j.clim.2025.110453_bb0245","doi-asserted-by":"crossref","DOI":"10.1016\/j.clim.2024.109901","article-title":"Favipiravir induces HuNoV viral mutagenesis and infectivity loss with clinical improvement in immunocompromised patients","volume":"259","author":"Kreins","year":"2024","journal-title":"Clin. Immunol."},{"key":"10.1016\/j.clim.2025.110453_bb0250","doi-asserted-by":"crossref","first-page":"322","DOI":"10.3389\/fimmu.2013.00322","article-title":"Immunodeficiency in DiGeorge syndrome and options for treating cases with complete Athymia","volume":"4","author":"Davies","year":"2013","journal-title":"Front. Immunol."},{"issue":"22","key":"10.1016\/j.clim.2025.110453_bb0255","doi-asserted-by":"crossref","first-page":"10778","DOI":"10.1073\/pnas.90.22.10778","article-title":"Human bone marrow and umbilical cord blood cells generate CD4+ and CD8+ single-positive T cells in murine fetal thymus organ culture","volume":"90","author":"Yeoman","year":"1993","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"43","key":"10.1016\/j.clim.2025.110453_bb0260","doi-asserted-by":"crossref","first-page":"7210","DOI":"10.1038\/sj.onc.1207941","article-title":"Identification of hematopoietic stem\/progenitor cells: strength and drawbacks of functional assays","volume":"23","author":"Coulombel","year":"2004","journal-title":"Oncogene"},{"issue":"6","key":"10.1016\/j.clim.2025.110453_bb0265","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1016\/S1074-7613(02)00474-0","article-title":"Induction of T cell development from hematopoietic progenitor cells by delta-like-1 in vitro","volume":"17","author":"Schmitt","year":"2002","journal-title":"Immunity"},{"key":"10.1016\/j.clim.2025.110453_bb0270","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/978-1-59745-182-6_9","article-title":"In vitro human T cell development directed by notch-ligand interactions","volume":"430","author":"Awong","year":"2008","journal-title":"Methods Mol. Biol."},{"issue":"2","key":"10.1016\/j.clim.2025.110453_bb0275","doi-asserted-by":"crossref","first-page":"867","DOI":"10.4049\/jimmunol.1000782","article-title":"Direct comparison of Dll1- and Dll4-mediated notch activation levels shows differential lymphomyeloid lineage commitment outcomes","volume":"185","author":"Mohtashami","year":"2010","journal-title":"J. Immunol."},{"issue":"3","key":"10.1016\/j.clim.2025.110453_bb0280","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/j.stem.2018.12.011","article-title":"Organoid-induced differentiation of conventional T cells from human pluripotent stem cells","volume":"24","author":"Montel-Hagen","year":"2019","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.clim.2025.110453_bb0285","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.omtn.2022.12.006","article-title":"Multiplex HDR for disease and correction modeling of SCID by CRISPR genome editing in human HSPCs","volume":"31","author":"Iancu","year":"2023","journal-title":"Mol. Ther. Nucleic Acids."},{"issue":"4","key":"10.1016\/j.clim.2025.110453_bb0290","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1182\/blood-2004-04-1293","article-title":"Induction of T-cell development from human cord blood hematopoietic stem cells by Delta-like 1 in vitro","volume":"105","author":"La Motte-Mohs","year":"2005","journal-title":"Blood"}],"container-title":["Clinical Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1521661625000282?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1521661625000282?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T20:03:31Z","timestamp":1778616211000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1521661625000282"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":57,"alternative-id":["S1521661625000282"],"URL":"https:\/\/doi.org\/10.1016\/j.clim.2025.110453","relation":{},"ISSN":["1521-6616"],"issn-type":[{"value":"1521-6616","type":"print"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Ex vivo T-lymphopoiesis assays assisting corrective treatment choice for genetically undefined T-lymphocytopenia","name":"articletitle","label":"Article Title"},{"value":"Clinical Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.clim.2025.110453","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"110453"}}