{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T20:00:10Z","timestamp":1758052810763,"version":"3.44.0"},"reference-count":21,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Clinical Biochemistry"],"published-print":{"date-parts":[[2004,1]]},"DOI":"10.1016\/j.clinbiochem.2003.09.007","type":"journal-article","created":{"date-parts":[[2003,10,31]],"date-time":"2003-10-31T17:05:07Z","timestamp":1067619907000},"page":"14-21","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"title":["Development of an ultrasensitive enzyme immunoassay for human proadrenomedullin N-terminal 20 peptide and direct measurement of two molecular forms of PAMP in plasma from healthy subjects and patients with cardiovascular disease"],"prefix":"10.1016","volume":"37","author":[{"given":"Seiichi","family":"Hashida","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Kitamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshitatsu","family":"Nagatomo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshisato","family":"Shibata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuroh","family":"Imamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shouichi","family":"Fujimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johji","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Morishita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tanenao","family":"Eto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB1","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1006\/bbrc.1993.1451","article-title":"Adrenomedullin: a novel hypotensive peptide isolated from human pheochromocytoma","volume":"192","author":"Kitamura","year":"1993","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB2","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/0014-5793(94)00810-8","article-title":"Identification and hypotensive activity of proadrenomedullin N-terminal 20 peptide (PAMP)","volume":"351","author":"Kitamura","year":"1994","journal-title":"FEBS Lett."},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB3","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1006\/bbrc.1995.1867","article-title":"Human proadrenomedullin N-terminal 20 peptide in pheochromocytoma and normal adrenal medulla","volume":"211","author":"Kuwasako","year":"1995","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB4","doi-asserted-by":"crossref","first-page":"1672","DOI":"10.1172\/JCI118208","article-title":"Proadrenomedullin NH2-terminal 20 peptide, a new product of the adrenomedullin gene, inhibits norepinephrine overflow from nerve endings","volume":"96","author":"Shimosawa","year":"1995","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB5","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1046\/j.1471-4159.1995.64010459.x","article-title":"Proadrenomedullin N-terminal 20 peptide (PAMP), an endogenous anticholinergic peptide: its exocytotic secretion, and inhibition of catecholamine secretion in adrenal medulla","volume":"64","author":"Katoh","year":"1995","journal-title":"J. Neurochem."},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB6","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1016\/S0196-9781(01)00513-7","article-title":"A review of the biological properties and clinical implications of adrenomedullin and proadrenomedullin N-terminal 20 peptide (PAMP), hypotensive and vasodilating peptides","volume":"22","author":"Eto","year":"2001","journal-title":"Peptides"},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB7","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1177\/000456329903600510","article-title":"Increased plasma proadrenomedullin N-terminal 20 peptide in patients with essential hypertension","volume":"36","author":"Kuwasako","year":"1999","journal-title":"Ann. Clin. Biochem."},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB8","first-page":"S148","article-title":"Adrenomedullin and proadrenomedullin N-terminal 20 peptide in impaired renal function","volume":"55","author":"Eto","year":"1996","journal-title":"Kidney Int."},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB9","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1002\/clc.4960220211","article-title":"Differential hormonal profiles of adrenomedullin and proadrenomedullin N-terminal 20 peptide in patients with heart failure and effect of treatment on their plasma levels","volume":"22","author":"Etoh","year":"1999","journal-title":"Clin. Cardiol."},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB10","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/S0272-6386(99)70116-2","article-title":"Plasma and urine levels of adrenomedullin and proadrenomedullin N-terminal 20 peptide in chronic glomerulonephritis","volume":"34","author":"Kinoshita","year":"1999","journal-title":"Am. J. Kidney Dis."},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB11","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1006\/bbrc.1998.8310","article-title":"The intermediate form of glycine-extended adrenomedullin is the major circulating molecular form in human plasma","volume":"244","author":"Kitamura","year":"1998","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB12","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1006\/bbrc.1994.2039","article-title":"Immunoreactive proadrenomedullin N-terminal 20 peptide in human tissue, plasma and urine","volume":"202","author":"Washimimine","year":"1994","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB13","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1093\/oxfordjournals.jbchem.a123321","article-title":"Detection of one milliattomole of ferritin by novel and ultrasensitive enzyme immunoassay","volume":"108","author":"Hashida","year":"1990","journal-title":"J. Biochem."},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB14","first-page":"403","article-title":"Ultrasensitive enzyme immunoassay","volume":"vol. 1","author":"Hashida","year":"1995"},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB15","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1126\/science.144.3624.1344","article-title":"Antibodies to bradykinin and angiotensin: a use of carbodiimides in immunology","volume":"144","author":"Goodfriend","year":"1964","journal-title":"Science"},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB16","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1080\/15321818308057011","article-title":"Enzyme-labeling of antibodies and their fragments for enzyme immunoassay and immunohistochemical staining","volume":"4","author":"Ishikawa","year":"1983","journal-title":"J. Immunoassay"},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB17","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1080\/00032718308064459","article-title":"A highly sensitive sandwich enzyme immunoassay of human growth hormone in serum using affinity-purified anti-human growth hormone Fab'-horseradish peroxidase conjugate","volume":"16","author":"Hashida","year":"1983","journal-title":"Anal. Lett."},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB18","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1128\/CDLI.7.6.872-881.2000","article-title":"Earlier detection of human immunodeficiency virus type 1 p24 antigen and immunoglobulin G and M antibodies to p17 antigen in seroconversion serum panels by immune complex transfer enzyme immunoassays","volume":"7","author":"Hashida","year":"2000","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB19","doi-asserted-by":"crossref","first-page":"C1908","DOI":"10.1152\/ajpcell.1997.273.6.C1908","article-title":"Amidative peptide processing and vascular function","volume":"273","author":"Oldham","year":"1997","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB20","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0167-0115(03)00002-8","article-title":"Glycine-extended adrenomedullin exerts vasodilator effect through amidation in the rat aorta","volume":"113","author":"Cao","year":"2003","journal-title":"Regul. Pept."},{"key":"10.1016\/j.clinbiochem.2003.09.007_BIB21","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1677\/joe.0.1600297","article-title":"Molecular forms of circulating adrenomedullin in patients with congestive heart failure","volume":"160","author":"Hirayama","year":"1999","journal-title":"J. Endocrinol."}],"container-title":["Clinical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009912003001607?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009912003001607?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,14]],"date-time":"2025-09-14T15:16:41Z","timestamp":1757863001000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0009912003001607"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["S0009912003001607"],"URL":"https:\/\/doi.org\/10.1016\/j.clinbiochem.2003.09.007","relation":{},"ISSN":["0009-9120"],"issn-type":[{"type":"print","value":"0009-9120"}],"subject":[],"published":{"date-parts":[[2004,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of an ultrasensitive enzyme immunoassay for human proadrenomedullin N-terminal 20 peptide and direct measurement of two molecular forms of PAMP in plasma from healthy subjects and patients with cardiovascular disease","name":"articletitle","label":"Article Title"},{"value":"Clinical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.clinbiochem.2003.09.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"converted-article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2003 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}