{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T19:59:01Z","timestamp":1758052741942,"version":"3.44.0"},"reference-count":15,"publisher":"Elsevier BV","issue":"4-5","license":[{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2012,3,1]],"date-time":"2012-03-01T00:00:00Z","timestamp":1330560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Clinical Biochemistry"],"published-print":{"date-parts":[[2012,3]]},"DOI":"10.1016\/j.clinbiochem.2011.12.026","type":"journal-article","created":{"date-parts":[[2012,1,12]],"date-time":"2012-01-12T13:46:24Z","timestamp":1326375984000},"page":"345-351","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["Four-channel asymmetric Real-Time PCR hybridization probe assay: A rapid pre-screening method for critical BCR-ABL kinase domain mutations"],"prefix":"10.1016","volume":"45","author":[{"given":"Jordi","family":"Martinez-Serra","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Guti\u00e9rrez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toni F.","family":"Marc\u00fas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Soverini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Carlos","family":"Amat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Navarro-Palou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Ros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Bex","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Ballester","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josep Miquel","family":"Bau\u00e7a","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"SanFelix","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9s","family":"Novo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Vidal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan","family":"Besalduch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.clinbiochem.2011.12.026_bb0005","first-page":"673","article-title":"What is Imatinib-resistant chronic myeloid leukemia? Identifying and managing loss of response","volume":"6","author":"Zeidan","year":"2008","journal-title":"Clin Adv Hematol Oncol"},{"key":"10.1016\/j.clinbiochem.2011.12.026_bb0010","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1182\/blood-2002-09-2896","article-title":"Detection of BCR-ABL mutations in patients with CML treated with Imatinib is virtually always accompanied by clinical resistance, and mutations in the ATP phosphate-binding loop (p-loop) are associated with a poor prognosis","volume":"102","author":"Branford","year":"2003","journal-title":"Blood"},{"key":"10.1016\/j.clinbiochem.2011.12.026_bb0015","first-page":"2205","article-title":"Resistance to tumor specific therapy with Imatinib by clonal selection of mutated cells","volume":"127","author":"Muller","year":"2002","journal-title":"Dtsch Med Wochenschr"},{"key":"10.1016\/j.clinbiochem.2011.12.026_bb0020","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1182\/blood-2010-12-326405","article-title":"BCR-ABL kinase domain mutation analysis in chronic myeloid leukemia patients treated with tyrosine kinase inhibitors: recommendations from an expert panel on behalf of European LeukemiaNet","volume":"118","author":"Soverini","year":"2011","journal-title":"Blood"},{"key":"10.1016\/j.clinbiochem.2011.12.026_bb0025","doi-asserted-by":"crossref","first-page":"1744","DOI":"10.1002\/cncr.23355","article-title":"Molecular basis explanation for Imatinib resistance of BCR-ABL due to T315I and p-loop mutations from molecular dynamics simulations","volume":"112","author":"Lee","year":"2008","journal-title":"Cancer"},{"key":"10.1016\/j.clinbiochem.2011.12.026_bb0030","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1158\/1078-0432.CCR-09-3314","article-title":"Targeting the BCR-ABL signaling pathway in therapy-resistant Philadelphia chromosome-positive leukemia","volume":"17","author":"O'Hare","year":"2011","journal-title":"Clin Cancer Res"},{"key":"10.1016\/j.clinbiochem.2011.12.026_bb0035","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.ccr.2009.09.028","article-title":"AP24534, a Pan-BCR-ABL inhibitor for chronic myeloid leukemia, potently inhibits the T315I mutant and overcomes mutation-based resistance","volume":"16","author":"O'Hare","year":"2009","journal-title":"Cancer Cell"},{"key":"10.1016\/j.clinbiochem.2011.12.026_bb0040","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1128\/JCM.40.4.1571-1572.2002","article-title":"Improving real-time PCR genotyping assays by asymmetric amplification","volume":"40","author":"Barratt","year":"2002","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.clinbiochem.2011.12.026_bb0045","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1016\/j.clinbiochem.2009.04.013","article-title":"Rapid triplex asymmetric real-time PCR hybridization probe assay for the joint genotyping of F2, F5 and F12","volume":"42","author":"Martinez-Serra","year":"2009","journal-title":"Clin Biochem"},{"key":"10.1016\/j.clinbiochem.2011.12.026_bb0050","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.cca.2009.09.022","article-title":"New real-time PCR-based method for the joint genotyping of JAK2 (V617F) with inherited thrombophilic F5 and F2 mutations","volume":"410","author":"Martinez-Serra","year":"2009","journal-title":"Clin Chim Acta"},{"key":"10.1016\/j.clinbiochem.2011.12.026_bb0055","doi-asserted-by":"crossref","first-page":"315","DOI":"10.3233\/CBM-2007-3605","article-title":"Detection of the JAK2V617F mutation by asymmetric PCR and melt curve analysis","volume":"3","author":"Sutton","year":"2007","journal-title":"Cancer Biomark"},{"key":"10.1016\/j.clinbiochem.2011.12.026_bb0060","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/j.clinbiochem.2005.04.015","article-title":"Asymmetric PCR increases efficiency of melting peak analysis on the LightCycler","volume":"38","author":"Szilvasi","year":"2005","journal-title":"Clin Biochem"},{"key":"10.1016\/j.clinbiochem.2011.12.026_bb0065","doi-asserted-by":"crossref","first-page":"669","DOI":"10.5858\/2006-130-669-IROAO","article-title":"Imatinib resistance: obstacles and opportunities","volume":"130","author":"Litzow","year":"2006","journal-title":"Arch Pathol Lab Med"},{"key":"10.1016\/j.clinbiochem.2011.12.026_bb0070","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1016\/j.leukres.2008.01.010","article-title":"High-resolution melt curve analysis: initial screening for mutations in BCR-ABL kinase domain","volume":"32","author":"Polakova","year":"2008","journal-title":"Leuk Res"},{"key":"10.1016\/j.clinbiochem.2011.12.026_bb0075","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1373\/clinchem.2004.031112","article-title":"Denaturing-HPLC-based assay for detection of ABL mutations in chronic myeloid leukemia patients resistant to Imatinib","volume":"50","author":"Soverini","year":"2004","journal-title":"Clin Chem"}],"container-title":["Clinical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009912012000057?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009912012000057?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,14]],"date-time":"2025-09-14T15:37:07Z","timestamp":1757864227000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0009912012000057"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3]]},"references-count":15,"journal-issue":{"issue":"4-5","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["S0009912012000057"],"URL":"https:\/\/doi.org\/10.1016\/j.clinbiochem.2011.12.026","relation":{},"ISSN":["0009-9120"],"issn-type":[{"type":"print","value":"0009-9120"}],"subject":[],"published":{"date-parts":[[2012,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Four-channel asymmetric Real-Time PCR hybridization probe assay: A rapid pre-screening method for critical BCR-ABL kinase domain mutations","name":"articletitle","label":"Article Title"},{"value":"Clinical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.clinbiochem.2011.12.026","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2012 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}