{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T20:19:53Z","timestamp":1775679593592,"version":"3.50.1"},"reference-count":35,"publisher":"Elsevier BV","issue":"15","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Clinical Biochemistry"],"published-print":{"date-parts":[[2013,10]]},"DOI":"10.1016\/j.clinbiochem.2013.04.012","type":"journal-article","created":{"date-parts":[[2013,4,23]],"date-time":"2013-04-23T21:03:55Z","timestamp":1366751035000},"page":"1601-1606","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"title":["The development and characterization of an ELISA specifically detecting the active form of cathepsin K"],"prefix":"10.1016","volume":"46","author":[{"given":"S.","family":"Sun","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.A.","family":"Karsdal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.C.","family":"Bay-Jensen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.G.","family":"S\u00f8rensen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q.","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.H.","family":"Dziegiel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.P.","family":"Maksymowych","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Henriksen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0005","doi-asserted-by":"crossref","first-page":"13453","DOI":"10.1073\/pnas.95.23.13453","article-title":"Impaired osteoclastic bone resorption leads to osteopetrosis in cathepsin-k-deficient mice","volume":"95","author":"Saftig","year":"1998","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0010","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1006\/bmme.1996.0088","article-title":"Cathepsin k: isolation and characterization of the murine cDNA and genomic sequence, the homologue of the human pycnodysostosis gene","volume":"59","author":"Gelb","year":"1996","journal-title":"Biochem Mol Med"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0015","doi-asserted-by":"crossref","first-page":"e27482","DOI":"10.1371\/journal.pone.0027482","article-title":"Dissociation of bone resorption and bone formation in adult mice with a non-functional v-atpase in osteoclasts leads to increased bone strength","volume":"6","author":"Henriksen","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0020","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1359\/jbmr.2001.16.3.478","article-title":"Human osteoclast cathepsin k is processed intracellularly prior to attachment and bone resorption","volume":"16","author":"Dodds","year":"2001","journal-title":"J Bone Miner Res"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0025","doi-asserted-by":"crossref","first-page":"12517","DOI":"10.1074\/jbc.271.21.12517","article-title":"Proteolytic activity of human osteoclast cathepsin k. Expression, purification, activation, and substrate identification","volume":"271","author":"Bossard","year":"1996","journal-title":"J Biol Chem"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0030","doi-asserted-by":"crossref","first-page":"13955","DOI":"10.1074\/jbc.272.21.13955","article-title":"Autocatalytic activation of human cathepsin k","volume":"272","author":"McQueney","year":"1997","journal-title":"J Biol Chem"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0035","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S8756-3282(96)00351-1","article-title":"Localization of cathepsin k in human osteoclasts by in situ hybridization and immunohistochemistry","volume":"20","author":"Littlewood-Evans","year":"1997","journal-title":"Bone"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0040","doi-asserted-by":"crossref","first-page":"12511","DOI":"10.1074\/jbc.271.21.12511","article-title":"Cathepsin k, but not cathepsins b, l, or s, is abundantly expressed in human osteoclasts","volume":"271","author":"Drake","year":"1996","journal-title":"J Biol Chem"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0045","doi-asserted-by":"crossref","first-page":"32347","DOI":"10.1074\/jbc.273.48.32347","article-title":"The collagenolytic activity of cathepsin k is unique among mammalian proteinases","volume":"273","author":"Garnero","year":"1998","journal-title":"J Biol Chem"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0050","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/S8756-3282(00)00235-0","article-title":"Immunochemical characterization of assay for carboxyterminal telopeptide of human type i collagen: Loss of antigenicity by treatment with cathepsin k","volume":"26","author":"Sassi","year":"2000","journal-title":"Bone"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0055","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1080\/13547500601070842","article-title":"Biochemical markers in preclinical models of osteoporosis","volume":"12","author":"Sorensen","year":"2007","journal-title":"Biomarkers"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0060","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.1359\/jbmr.1999.14.11.1902","article-title":"Determination of bone markers in pycnodysostosis: effects of cathepsin k deficiency on bone matrix degradation","volume":"14","author":"Nishi","year":"1999","journal-title":"J Bone Miner Res"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0065","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1359\/jbmr.1999.14.10.1654","article-title":"Cathepsin k knockout mice develop osteopetrosis due to a deficit in matrix degradation but not demineralization","volume":"14","author":"Gowen","year":"1999","journal-title":"J Bone Miner Res"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0070","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1359\/jbmr.091035","article-title":"Odanacatib, a cathepsin-k inhibitor for osteoporosis: a two-year study in postmenopausal women with low bone density","volume":"25","author":"Bone","year":"2010","journal-title":"J Bone Miner Res"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0075","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1002\/jbmr.212","article-title":"Odanacatib in the treatment of postmenopausal women with low bone mineral density: three-year continued therapy and resolution of effect","volume":"26","author":"Eisman","year":"2011","journal-title":"J Bone Miner Res"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0080","first-page":"1","article-title":"Serum cathepsin k concentrations reflect osteoclastic activity in women with postmenopausal osteoporosis and patients with Paget's disease","volume":"52","author":"Meier","year":"2006","journal-title":"Clin Lab"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0085","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/j.exger.2005.04.001","article-title":"Serum levels of cathepsin k decrease with age in both women and men","volume":"40","author":"Kerschan-Schindl","year":"2005","journal-title":"Exp Gerontol"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0090","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1097\/gme.0b013e3181271873","article-title":"Cathepsin k predicts femoral neck bone mineral density change in nonosteoporotic peri- and early postmenopausal women","volume":"15","author":"Prezelj","year":"2008","journal-title":"Menopause"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0095","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.maturitas.2009.09.011","article-title":"Serum cathepsin k as a marker of bone metabolism in postmenopausal women treated with alendronate","volume":"64","author":"Munoz-Torres","year":"2009","journal-title":"Maturitas"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0100","doi-asserted-by":"crossref","first-page":"R65","DOI":"10.1186\/ar1461","article-title":"Serum cathepsin k levels of patients with longstanding rheumatoid arthritis: correlation with radiological destruction","volume":"7","author":"Skoumal","year":"2005","journal-title":"Arthritis Res Ther"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0105","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.jbspin.2008.01.026","article-title":"Serum levels of mmp-3 and cathepsin k in patients with ankylosing spondylitis: effect of tnfalpha antagonist therapy","volume":"75","author":"Wendling","year":"2008","journal-title":"Joint Bone Spine"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0110","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1016\/j.clinbiochem.2010.03.012","article-title":"A novel assay for extracellular matrix remodeling associated with liver fibrosis: an enzyme-linked immunosorbent assay (ELISA) for a mmp-9 proteolytically revealed neo-epitope of type iii collagen","volume":"43","author":"Barascuk","year":"2010","journal-title":"Clin Biochem"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0115","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/BF01551818","article-title":"A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells","volume":"3","author":"Gefter","year":"1977","journal-title":"Somatic Cell Genet"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0120","doi-asserted-by":"crossref","first-page":"44975","DOI":"10.1074\/jbc.M303905200","article-title":"Transforming growth factor-beta controls human osteoclastogenesis through the p38 mapk and regulation of rank expression","volume":"278","author":"Karsdal","year":"2003","journal-title":"J Biol Chem"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0125","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1046\/j.1365-2141.1999.01491.x","article-title":"Human osteoclasts derive from cd14-positive monocytes","volume":"106","author":"Massey","year":"1999","journal-title":"Br J Haematol"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0130","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1042\/cs0990133","article-title":"Induction of osteoclasts from cd14-positive human peripheral blood mononuclear cells by receptor activator of nuclear factor kappab ligand (rankl)","volume":"99","author":"Nicholson","year":"2000","journal-title":"Clin Sci (Lond)"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0135","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1080\/13697130310001651535","article-title":"Safety and efficacy of drospirenone used in a continuous combination with 17beta-estradiol for prevention of postmenopausal osteoporosis","volume":"7","author":"Warming","year":"2004","journal-title":"Climacteric"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0140","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1016\/j.clinbiochem.2010.07.025","article-title":"Enzyme-linked immunosorbent serum assays (ELISAS) for rat and human n-terminal pro-peptide of collagen type i (PINP) \u2014 assessment of corresponding epitopes","volume":"43","author":"Leeming","year":"2010","journal-title":"Clin Biochem"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0145","doi-asserted-by":"crossref","first-page":"1846","DOI":"10.1002\/art.22589","article-title":"Serum matrix metalloproteinase 3 is an independent predictor of structural damage progression in patients with ankylosing spondylitis","volume":"56","author":"Maksymowych","year":"2007","journal-title":"Arthritis Rheum"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0150","doi-asserted-by":"crossref","first-page":"3970","DOI":"10.1021\/bi9523015","article-title":"Contribution to activity of histidine\u2013aromatic, amide\u2013aromatic, and aromatic\u2013aromatic interactions in the extended catalytic site of cysteine proteinases","volume":"35","author":"Bromme","year":"1996","journal-title":"Biochemistry"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0155","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1002\/jbmr.341","article-title":"Safety and efficacy of the cathepsin k inhibitor ono-5334 in postmenopausal osteoporosis: the ocean study","volume":"26","author":"Eastell","year":"2011","journal-title":"J Bone Miner Res"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0160","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1016\/S0002-9440(10)63712-1","article-title":"Characterization of osteoclasts from patients harboring a g215r mutation in clc-7 causing autosomal dominant osteopetrosis type ii","volume":"164","author":"Henriksen","year":"2004","journal-title":"Am J Pathol"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0165","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.biochi.2007.08.011","article-title":"Biochemical properties and regulation of cathepsin k activity","volume":"90","author":"Lecaille","year":"2008","journal-title":"Biochimie"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0170","doi-asserted-by":"crossref","first-page":"27723","DOI":"10.1074\/jbc.M111.332684","article-title":"Cathepsin s cannibalism of cathepsin k as a mechanism to reduce type i collagen degradation","volume":"287","author":"Barry","year":"2012","journal-title":"J Biol Chem"},{"key":"10.1016\/j.clinbiochem.2013.04.012_bb0175","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.maturitas.2011.11.024","article-title":"Serum cathepsin k levels are not suitable to differentiate women with chronic bone disorders such as osteopenia and osteoporosis from healthy pre- and postmenopausal women","volume":"71","author":"Adolf","year":"2012","journal-title":"Maturitas"}],"container-title":["Clinical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009912013001525?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009912013001525?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,14]],"date-time":"2025-09-14T15:46:20Z","timestamp":1757864780000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0009912013001525"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10]]},"references-count":35,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2013,10]]}},"alternative-id":["S0009912013001525"],"URL":"https:\/\/doi.org\/10.1016\/j.clinbiochem.2013.04.012","relation":{},"ISSN":["0009-9120"],"issn-type":[{"value":"0009-9120","type":"print"}],"subject":[],"published":{"date-parts":[[2013,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The development and characterization of an ELISA specifically detecting the active form of cathepsin K","name":"articletitle","label":"Article Title"},{"value":"Clinical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.clinbiochem.2013.04.012","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}