{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:24:01Z","timestamp":1761744241777,"version":"3.44.0"},"reference-count":32,"publisher":"Elsevier BV","issue":"7-8","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Clinical Biochemistry"],"published-print":{"date-parts":[[2016,5]]},"DOI":"10.1016\/j.clinbiochem.2015.12.011","type":"journal-article","created":{"date-parts":[[2015,12,29]],"date-time":"2015-12-29T12:02:20Z","timestamp":1451390540000},"page":"580-586","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":20,"title":["Quantification of darunavir and etravirine in human peripheral blood mononuclear cells using high performance liquid chromatography tandem mass spectrometry (LC\u2013MS\/MS), clinical application in a cohort of 110 HIV-1 infected patients and evidence of a potential drug\u2013drug interaction"],"prefix":"10.1016","volume":"49","author":[{"given":"Le\u00efla","family":"Belkhir","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morgane","family":"De Laveleye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Vandercam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis","family":"Zech","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin-Alexandre","family":"Delongie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnaud","family":"Capron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Yombi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Vincent","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laure","family":"Elens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Haufroid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0005","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/s40265-013-0159-3","article-title":"Darunavir: a review of its use in the management of HIV-1 infection","volume":"74","author":"Deeks","year":"2014","journal-title":"Drugs"},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0010","doi-asserted-by":"crossref","first-page":"477","DOI":"10.2165\/00003495-200969040-00007","article-title":"Darunavir: a review of its use in the management of HIV infection in adults","volume":"69","author":"McKeage","year":"2009","journal-title":"Drugs"},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0015","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1016\/j.clinthera.2009.04.020","article-title":"Etravirine: a second-generation nonnucleoside reverse transcriptase inhibitor (NNRTI) active against NNRTI-resistant strains of HIV","volume":"31","author":"Schiller","year":"2009","journal-title":"Clin. Ther."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0020","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1468-1293.2011.00971.x","article-title":"British HIV Association guidelines for the routine investigation and monitoring of adult HIV-1-infected individuals 2011","volume":"13","author":"Asboe","year":"2012","journal-title":"HIV Med."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0025","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1097\/00002030-200101050-00011","article-title":"Efavirenz plasma levels can predict treatment failure and central nervous system side effects in HIV-1-infected patients","volume":"15","author":"Marzolini","year":"2001","journal-title":"AIDS"},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0030","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1097\/FTD.0b013e3181c05adf","article-title":"Validation of an electrospray ionization LC\u2013MS\/MS method for quantitative analysis of raltegravir, etravirine, and 9 other antiretroviral agents in human plasma samples","volume":"31","author":"Quaranta","year":"2009","journal-title":"Ther. Drug Monit."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0035","doi-asserted-by":"crossref","first-page":"17","DOI":"10.2165\/11318110-000000000-00000","article-title":"Intracellular pharmacokinetics of antiretroviral drugs in hiv-infected patients, and their correlation with drug action","volume":"49","author":"Bazzoli","year":"2010","journal-title":"Clin. Pharmacokinet."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0040","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1016\/j.jchromb.2009.04.046","article-title":"Validation and clinical application of a high performance liquid chromatography tandem mass spectrometry (LC\u2013MS\/MS) method for the quantitative determination of 10 anti-retrovirals in human peripheral blood mononuclear cells","volume":"877","author":"Elens","year":"2009","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0045","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1093\/jac\/dkh487","article-title":"The intracellular pharmacology of antiretroviral protease inhibitors","volume":"54","author":"Ford","year":"2004","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0050","doi-asserted-by":"crossref","first-page":"1588","DOI":"10.1248\/bpb.32.1588","article-title":"P-glycoprotein mediates efflux transport of darunavir in human intestinal caco-2 and abcb1 gene-transfected renal llc-pk1 cell lines","volume":"32","author":"Fujimoto","year":"2009","journal-title":"Biol. Pharm. Bull."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0055","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1097\/QAI.0b013e3182364c67","article-title":"Plasma and intracellular pharmacokinetics of darunavir\/ritonavir once daily and raltegravir once and twice daily in HIV-infected individuals","volume":"58","author":"Jackson","year":"2011","journal-title":"J. Acquir. Immune Defic. Syndr."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0060","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/j.intimp.2013.05.003","article-title":"Determination of p-glycoprotein surface expression and functional ability after in vitro treatment with darunavir or raltegravir in lymphocytes of healthy donors","volume":"16","author":"Tempestilli","year":"2013","journal-title":"Int. Immunopharmacol."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0065","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1016\/j.jpba.2010.10.011","article-title":"A HPLC\u2013MS method for the simultaneous quantification of fourteen antiretroviral agents in peripheral blood mononuclear cell of HIV infected patients optimized using medium corpuscular volume evaluation","volume":"54","author":"D'Avolio","year":"2011","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0070","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1111\/j.1365-2125.2010.03634.x","article-title":"Intracellular and plasma steady-state pharmacokinetics of raltegravir, darunavir, etravirine and ritonavir in heavily pre-treated HIV-infected patients","volume":"69","author":"Ter Heine","year":"2010","journal-title":"Br. J. Clin. Pharmacol."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0075","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1093\/jac\/dki308","article-title":"Intracellular and plasma pharmacokinetics of efavirenz in HIV-infected individuals","volume":"56","author":"Almond","year":"2005","journal-title":"J. Antimicrob. Chemother."},{"article-title":"US Department of Health and Human Services FDA, guidance for industry","year":"2000","series-title":"Analytical procedures and methods validation","key":"10.1016\/j.clinbiochem.2015.12.011_bb0080"},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0085","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.clinbiochem.2004.11.007","article-title":"Matrix effects: The achilles heel of quantitative high-performance liquid chromatography-electrospray-tandem mass spectrometry","volume":"38","author":"Taylor","year":"2005","journal-title":"Clin. Biochem."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0090","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1373\/clinchem.2008.108647","article-title":"Quantification of 8 HIV-protease inhibitors and 2 nonnucleoside reverse transcriptase inhibitors by ultra-performance liquid chromatography with diode array detection","volume":"55","author":"Elens","year":"2009","journal-title":"Clin. Chem."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0095","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S0378-3758(98)00180-3","article-title":"On eliminating the asymptotic bias in the quasi-least squares estimate of the correlation parameter","volume":"76","author":"Chaganty","year":"1999","journal-title":"J. Stat. Plan. Infer."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0100","doi-asserted-by":"crossref","first-page":"3479","DOI":"10.1002\/sim.2161","article-title":"Small-sample performance of the robust score test and its modifications in generalized estimating equations","volume":"24","author":"Guo","year":"2005","journal-title":"Stat. Med."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0105","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1093\/chromsci\/bmt147","article-title":"Determination of intracellular darunavir by liquid chromatography coupled with fluorescence detection","volume":"52","author":"Nagano","year":"2014","journal-title":"J. Chromatogr. Sci."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0110","doi-asserted-by":"crossref","first-page":"2866","DOI":"10.1128\/AAC.02551-13","article-title":"Quantification of cell-associated atazanavir, darunavir, lopinavir, ritonavir, and efavirenz concentrations in human mononuclear cell extracts","volume":"58","author":"Podany","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0115","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1097\/FTD.0b013e31829ad690","article-title":"Interindividual and intra-individual variabilities of darunavir and ritonavir plasma trough concentrations in multidrug experienced hiv patients receiving salvage regimens","volume":"35","author":"Baroncelli","year":"2013","journal-title":"Ther. Drug Monit."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0120","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1097\/QAD.0b013e3283553619","article-title":"Determinants of darunavir cerebrospinal fluid concentrations: Impact of once-daily dosing and pharmacogenetics","volume":"26","author":"Calcagno","year":"2012","journal-title":"AIDS"},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0125","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1097\/01.aids.0000194793.36175.40","article-title":"Modulation of the intracellular accumulation of saquinavir in peripheral blood mononuclear cells by inhibitors of mrp1, mrp2, p-gp and bcrp","volume":"19","author":"Janneh","year":"2005","journal-title":"AIDS"},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0130","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1097\/00002030-200104130-00002","article-title":"Differences in the intracellular accumulation of HIV protease inhibitors in vitro and the effect of active transport","volume":"15","author":"Jones","year":"2001","journal-title":"AIDS"},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0135","doi-asserted-by":"crossref","first-page":"3228","DOI":"10.1128\/AAC.46.10.3228-3235.2002","article-title":"Intracellular accumulation of human immunodeficiency virus protease inhibitors","volume":"46","author":"Khoo","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0140","doi-asserted-by":"crossref","first-page":"561","DOI":"10.2165\/10895940-000000000-00000","article-title":"Clinical pharmacokinetics and pharmacodynamics of etravirine","volume":"48","author":"Scholler-Gyure","year":"2009","journal-title":"Clin. Pharmacokinet."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0145","series-title":"8th International Congress on Drug Therapy in HIV infection","first-page":"278","article-title":"Pharmacokinetics of tmc125 with atazanavir and atazanavir\/ritonavir","author":"Sch\u00f6ller-Gy\u00fcre","year":"2006"},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0150","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1124\/dmd.108.024109","article-title":"Absorption, metabolism, and excretion of darunavir, a new protease inhibitor, administered alone and with low-dose ritonavir in healthy subjects","volume":"37","author":"Vermeir","year":"2009","journal-title":"Drug Metab. Dispos."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0155","doi-asserted-by":"crossref","first-page":"137","DOI":"10.2165\/00003088-200645020-00002","article-title":"Fosamprenavir: clinical pharmacokinetics and drug interactions of the amprenavir prodrug","volume":"45","author":"Wire","year":"2006","journal-title":"Clin. Pharmacokinet."},{"key":"10.1016\/j.clinbiochem.2015.12.011_bb0160","series-title":"46th Interscience Conference on Antimicrobial Agents and Chemotherapy","article-title":"Pharmacokinetics of amprenavir and tmc125 in HIV-infected volunteers receiving tmc125 with fosamprenavir\/ritonavir","author":"Sch\u00f6ller-Gy\u00fcre","year":"2006"}],"container-title":["Clinical Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009912015005895?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0009912015005895?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,14]],"date-time":"2025-09-14T15:47:33Z","timestamp":1757864853000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0009912015005895"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5]]},"references-count":32,"journal-issue":{"issue":"7-8","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["S0009912015005895"],"URL":"https:\/\/doi.org\/10.1016\/j.clinbiochem.2015.12.011","relation":{},"ISSN":["0009-9120"],"issn-type":[{"type":"print","value":"0009-9120"}],"subject":[],"published":{"date-parts":[[2016,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Quantification of darunavir and etravirine in human peripheral blood mononuclear cells using high performance liquid chromatography tandem mass spectrometry (LC\u2013MS\/MS), clinical application in a cohort of 110 HIV-1 infected patients and evidence of a potential drug\u2013drug interaction","name":"articletitle","label":"Article Title"},{"value":"Clinical Biochemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.clinbiochem.2015.12.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}