{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T16:42:19Z","timestamp":1788280939326,"version":"build-2803163510"},"reference-count":34,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Clinical Neurology and Neurosurgery"],"published-print":{"date-parts":[[2017,10]]},"DOI":"10.1016\/j.clineuro.2017.08.013","type":"journal-article","created":{"date-parts":[[2017,8,31]],"date-time":"2017-08-31T09:16:50Z","timestamp":1504171010000},"page":"110-116","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"C","title":["Use of multiplex PCR based molecular diagnostics in diagnosis of suspected CNS infections in tertiary care setting\u2014A retrospective study"],"prefix":"10.1016","volume":"161","author":[{"given":"Mahendra","family":"Javali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Purushottam","family":"Acharya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aneesh","family":"Mehta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aju Abraham","family":"John","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohan","family":"Mahale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Srinivasa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.clineuro.2017.08.013_bib0005","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1128\/JCM.35.3.691-696.1997","article-title":"Aseptic meningitis and encephalitis: the role of PCR in the diagnostic laboratory","volume":"35","author":"Read","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0010","doi-asserted-by":"crossref","first-page":"2127","DOI":"10.1128\/JCM.37.7.2127-2136.1999","article-title":"Molecular diagnosis of herpes simplex virus infections in the central nervous system","volume":"37","author":"Tang","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0015","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1128\/JCM.34.1.199-201.1996","article-title":"Rapid and sensitive detection of enteroviruses in specimens from aseptic meningitis","volume":"34","author":"Yerly","year":"1996","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0020","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1128\/CMR.17.4.903-925.2004","article-title":"Molecular methods for diagnosis of viral encephalitis","volume":"17","author":"Roberta","year":"2004","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0025","doi-asserted-by":"crossref","first-page":"3056","DOI":"10.1128\/JCM.39.9.3056-3059.2001","article-title":"LightCycler multiplex PCR for the laboratory diagnosis of common viral infections of the central nervous system","volume":"39","author":"Read","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0030","doi-asserted-by":"crossref","first-page":"1352","DOI":"10.1128\/JCM.37.5.1352-1355.1999","article-title":"Laboratory diagnosis of common viral infections of the central nervous system by using a single multiplex PCR screening assay","volume":"37","author":"Read","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0035","doi-asserted-by":"crossref","first-page":"4426","DOI":"10.1128\/JCM.39.12.4426-4432.2001","article-title":"Laboratory diagnosis of common herpesvirus infections of the central nervous system by a multiplex PCR assay","volume":"39","author":"Markoulatos","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0040","doi-asserted-by":"crossref","first-page":"3874","DOI":"10.1128\/JCM.01214-11","article-title":"Rapid virological diagnosis of central nervous system infections by use of a multiplex reverse transcription-PCR DNA microarray","volume":"49","author":"Leveque","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0045","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.mcp.2011.03.004","article-title":"Single tube multiplex real-time PCR for the rapid detection of herpesvirus infections of the central nervous system","volume":"25","author":"Sankuntaw","year":"2011","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.clineuro.2017.08.013_bib0050","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.diagmicrobio.2012.02.015","article-title":"Evaluation of multiplex polymerase chain reaction and microarray-based assay for rapid herpesvirus diagnostics","volume":"73","author":"Mannonen","year":"2012","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0055","first-page":"143","article-title":"Simultaneous detection of viruses and Toxoplasma gondii in cerebrospinal fluid specimens by multiplex polymerase chain reaction-based reverse hybridization assay","volume":"32","author":"Del Prete","year":"2009","journal-title":"New Microbiol."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0060","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1139\/w07-074","article-title":"Development of a multiplex polymerase chain reaction for detection and typing of major human herpesviruses in cerebrospinal fluid","volume":"53","author":"Bergallo","year":"2007","journal-title":"Can. J. Microbiol."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0065","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.jcv.2004.05.018","article-title":"Development of a multiplex nested consensus PCR for detection and identification of major human herpesviruses in CNS infections","volume":"32","author":"Tafreshi","year":"2005","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0070","doi-asserted-by":"crossref","first-page":"102","DOI":"10.4103\/0255-0857.81777","article-title":"Comparison of a conventional polymerase chain reaction with real-time polymerase chain reaction for the detection of neurotropic viruses in cerebrospinal fluid samples","volume":"29","author":"Ramamurthy","year":"2011","journal-title":"Indian J. Med. Microbiol."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0075","first-page":"182","article-title":"Application of 16S rDNA based seminested PCR for diagnosis of acute bacterial meningitis","volume":"129","author":"Chakrabarti","year":"2009","journal-title":"Indian J. Med. Res."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0080","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1006\/mcpr.2000.0287","article-title":"Rapid diagnosis of acute pyogenic meningitis by a combined PCR dot-blot assay","volume":"14","author":"Balganesh","year":"2000","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.clineuro.2017.08.013_bib0085","first-page":"73","article-title":"Bacterial meningitis: a five year (2001\u20132005) retrospective study at university malaya medical center (UMMC), Kualalumpur, Malaysia","volume":"39","author":"Erleena Nur","year":"2008","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"10.1016\/j.clineuro.2017.08.013_bib0090","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1056\/NEJMra052116","article-title":"Community-acquired bacterial meningitis in adults","volume":"354","author":"Van de Beek","year":"2006","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0095","series-title":"Clinical Information for Healthcare Professionals","author":"Centers for Disease Control and Prevention Meningitis","year":"2016"},{"key":"10.1016\/j.clineuro.2017.08.013_bib0100","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1086\/425368","article-title":"Practice guidelines for the management of bacterial meningitis","volume":"39","author":"Tunkel","year":"2004","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0105","first-page":"914","article-title":"Diagnosis and outcome of acute bacterial meningitis in early childhood","volume":"39","author":"Chinchankar","year":"2002","journal-title":"Indian Pediatr."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0110","doi-asserted-by":"crossref","first-page":"197","DOI":"10.7883\/yoken.67.197","article-title":"Epidemiology and etiology of acute encephalitis syndrome in North India","volume":"67","author":"Jain","year":"2014","journal-title":"Jpn. J. Infect. Dis."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0115","series-title":"External Quality Assessment Program from Quality Control for Molecular Diagnostics","year":"2015"},{"key":"10.1016\/j.clineuro.2017.08.013_bib0120","doi-asserted-by":"crossref","first-page":"413","DOI":"10.3109\/02688697.2015.1006168","article-title":"Methicillin-Resistant Staphylococcus aureus central nervous system infections \u2212 Analysis and outcome","volume":"29","author":"Kumari","year":"2015","journal-title":"Br. J. Neurosurg."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0125","first-page":"116","article-title":"Central nervous system tuberculosis","volume":"11","author":"Cherian","year":"2011","journal-title":"Afr. Health Sci."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0130","doi-asserted-by":"crossref","first-page":"324","DOI":"10.4103\/0976-9668.136176","article-title":"Prevalence of cryptococcal meningitis among people living with human immunodeficiency virus\/acquired immunodeficiency syndrome in a Tertiary Care Hospital, Southern Odisha, India","volume":"5","author":"Dash","year":"2014","journal-title":"J. Nat. Sci. Biol. Med."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0135","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.1016\/j.jocn.2014.08.001","article-title":"Cryptococcal ventriculoperitoneal shunt infection","volume":"21","author":"Viereck","year":"2014","journal-title":"J. Clin. Neurosci."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0140","article-title":"Viral infections of the central nervous system \u2013 use of a multiplex pcr microarray for its diagnosis","author":"Correia","year":"2012","journal-title":"15th Annual Meeting of the European Society for Clinical Virology (ESCV) and Joint Meeting with the European Society for Veterinary Virology (ESVV) 2012"},{"key":"10.1016\/j.clineuro.2017.08.013_bib0145","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1136\/jnnp.51.7.998","article-title":"Polymicrobial central nervous system infection in the acquired immunodeficiency syndrome","volume":"51","author":"Sperber","year":"1988","journal-title":"J. Neurol. Neurosurg. Psychiatry"},{"key":"10.1016\/j.clineuro.2017.08.013_bib0150","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1046\/j.1469-0691.2003.00651.x","article-title":"A twelve-year review of central nervous system bacterial abscesses; presentation and aetiology","volume":"9","author":"Roche","year":"2003","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0155","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.jmii.2011.12.008","article-title":"Mastoiditis diagnosed by clinical symptoms and imaging studies in children: disease spectrum and evolving diagnostic challenges","volume":"45","author":"Chien","year":"2012","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0160","first-page":"348","article-title":"Myth: cerebrospinal fluid analysis can differentiate bacterial meningitis from aseptic meningitis","volume":"5","author":"Graham","year":"2003","journal-title":"CJEM"},{"key":"10.1016\/j.clineuro.2017.08.013_bib0165","doi-asserted-by":"crossref","first-page":"307","DOI":"10.4103\/0022-3859.70939","article-title":"Multiple ring-enhancing lesions of the brain","volume":"56","author":"Garg","year":"2010","journal-title":"J. Postgrad. Med."},{"key":"10.1016\/j.clineuro.2017.08.013_bib0170","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1007\/s12098-015-1956-3","article-title":"Syndrome evaluation system (SES) versus blood culture (BACTEC) in the diagnosis and management of neonatal Sepsis\u2013A randomized controlled trial","volume":"83","author":"Bhat","year":"2016","journal-title":"Indian J. Pediatr."}],"container-title":["Clinical Neurology and Neurosurgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0303846717302329?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0303846717302329?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T19:11:24Z","timestamp":1602875484000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0303846717302329"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10]]},"references-count":34,"alternative-id":["S0303846717302329"],"URL":"https:\/\/doi.org\/10.1016\/j.clineuro.2017.08.013","relation":{},"ISSN":["0303-8467"],"issn-type":[{"value":"0303-8467","type":"print"}],"subject":[],"published":{"date-parts":[[2017,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Use of multiplex PCR based molecular diagnostics in diagnosis of suspected CNS infections in tertiary care setting\u2014A retrospective study","name":"articletitle","label":"Article Title"},{"value":"Clinical Neurology and Neurosurgery","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.clineuro.2017.08.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}