{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T08:15:58Z","timestamp":1761898558496,"version":"build-2065373602"},"reference-count":31,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2020,2,1]],"date-time":"2020-02-01T00:00:00Z","timestamp":1580515200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","em-consulte.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Clinics and Research in Hepatology and Gastroenterology"],"published-print":{"date-parts":[[2020,2]]},"DOI":"10.1016\/j.clinre.2019.04.003","type":"journal-article","created":{"date-parts":[[2019,5,8]],"date-time":"2019-05-08T21:13:19Z","timestamp":1557349999000},"page":"12-20","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"title":["Improved quantitative assessment of HBV-associated liver fibrosis using second-harmonic generation microscopy with feature selection"],"prefix":"10.1016","volume":"44","author":[{"given":"C.-Y.","family":"Hsiao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"Teng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.-H.","family":"Su","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.-H.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.-H.","family":"Kao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.-W.","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.clinre.2019.04.003_bib0005","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1016\/S0140-6736(14)60220-8","article-title":"Hepatitis B virus infection","volume":"384","author":"Trepo","year":"2014","journal-title":"Lancet"},{"issue":"Suppl 1","key":"10.1016\/j.clinre.2019.04.003_bib0010","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1111\/j.1478-3231.2008.01941.x","article-title":"Natural history of chronic hepatitis B virus infection and long-term outcome under treatment","volume":"29","author":"Liaw","year":"2009","journal-title":"Liver Int"},{"key":"10.1016\/j.clinre.2019.04.003_bib0015","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.jhep.2012.02.033","article-title":"Hepatitis B virus-related decompensated liver cirrhosis: benefits of antiviral therapy","volume":"57","author":"Peng","year":"2012","journal-title":"J Hepatol"},{"key":"10.1016\/j.clinre.2019.04.003_bib0020","doi-asserted-by":"crossref","first-page":"1486","DOI":"10.1056\/NEJMra0801644","article-title":"Hepatitis B virus infection","volume":"359","author":"Dienstag","year":"2008","journal-title":"N Engl J Med"},{"key":"10.1016\/j.clinre.2019.04.003_bib0025","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1136\/gut.46.4.443","article-title":"Is liver fibrosis reversible?","volume":"46","author":"Benyon","year":"2000","journal-title":"Gut"},{"key":"10.1016\/j.clinre.2019.04.003_bib0030","doi-asserted-by":"crossref","first-page":"2247","DOI":"10.1074\/jbc.275.4.2247","article-title":"Molecular regulation of hepatic fibrosis, an integrated cellular response to tissue injury","volume":"275","author":"Friedman","year":"2000","journal-title":"J Biol Chem"},{"key":"10.1016\/j.clinre.2019.04.003_bib0035","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1172\/JCI24282","article-title":"Liver fibrosis","volume":"115","author":"Bataller","year":"2005","journal-title":"J Clin Invest"},{"key":"10.1016\/j.clinre.2019.04.003_bib0040","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1016\/j.gcb.2009.06.001","article-title":"Hepatitis B: liver fibrosis and hepatocellular carcinoma","volume":"33","author":"Lok","year":"2009","journal-title":"Gastroenterol Clin Biol"},{"key":"10.1016\/j.clinre.2019.04.003_bib0045","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1002\/hep.23785","article-title":"Long-term entecavir therapy results in the reversal of fibrosis\/cirrhosis and continued histological improvement in patients with chronic hepatitis B","volume":"52","author":"Chang","year":"2010","journal-title":"Hepatology"},{"key":"10.1016\/j.clinre.2019.04.003_bib0050","doi-asserted-by":"crossref","first-page":"h4263","DOI":"10.1136\/bmj.h4263","article-title":"Management of chronic hepatitis B infection","volume":"351","author":"Sundaram","year":"2015","journal-title":"BMJ"},{"key":"10.1016\/j.clinre.2019.04.003_bib0055","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/0168-8278(95)80226-6","article-title":"Histological grading and staging of chronic hepatitis","volume":"22","author":"Ishak","year":"1995","journal-title":"J Hepatol"},{"key":"10.1016\/j.clinre.2019.04.003_bib0060","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1002\/hep.510240201","article-title":"An algorithm for the grading of activity in chronic hepatitis C","volume":"24","author":"Bedossa","year":"1996","journal-title":"Hepatology"},{"key":"10.1016\/j.clinre.2019.04.003_bib0065","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1002\/hep.1840200104","article-title":"Intraobserver and interobserver variations in liver biopsy interpretation in patients with chronic hepatitis C","volume":"20","year":"1994","journal-title":"Hepatology"},{"key":"10.1016\/j.clinre.2019.04.003_bib0070","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1111\/j.1478-3231.1999.tb00033.x","article-title":"Interobserver study of liver histopathology using the Ishak score in patients with chronic hepatitis C virus infection","volume":"19","author":"Westin","year":"1999","journal-title":"Liver"},{"key":"10.1016\/j.clinre.2019.04.003_bib0075","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1046\/j.1365-2893.2002.00389.x","article-title":"Interobserver variation in interpretation of serial liver biopsies from patients with chronic hepatitis C","volume":"9","author":"Gronbaek","year":"2002","journal-title":"J Viral Hepat"},{"key":"10.1016\/j.clinre.2019.04.003_bib0080","doi-asserted-by":"crossref","first-page":"064010","DOI":"10.1117\/1.3041159","article-title":"Nonlinear optical microscopy: use of second harmonic generation and two-photon microscopy for automated quantitative liver fibrosis studies","volume":"13","author":"Sun","year":"2008","journal-title":"J Biomed Opt"},{"key":"10.1016\/j.clinre.2019.04.003_bib0085","doi-asserted-by":"crossref","first-page":"044013","DOI":"10.1117\/1.3183811","article-title":"Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy","volume":"14","author":"Tai","year":"2009","journal-title":"J Biomed Opt"},{"key":"10.1016\/j.clinre.2019.04.003_bib0090","doi-asserted-by":"crossref","first-page":"12962","DOI":"10.1038\/srep12962","article-title":"Automated biphasic morphological assessment of hepatitis B-related liver fibrosis using second harmonic generation microscopy","volume":"5","author":"Wang","year":"2015","journal-title":"Sci Rep"},{"key":"10.1016\/j.clinre.2019.04.003_bib0095","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.jhep.2009.12.009","article-title":"Fibrillar collagen scoring by second harmonic microscopy: a new tool in the assessment of liver fibrosis","volume":"52","author":"Gailhouste","year":"2010","journal-title":"J Hepatol"},{"key":"10.1016\/j.clinre.2019.04.003_bib0100","doi-asserted-by":"crossref","first-page":"25794","DOI":"10.1364\/OE.18.025794","article-title":"A robust collagen scoring method for human liver fibrosis by second harmonic microscopy","volume":"18","author":"Guilbert","year":"2010","journal-title":"Opt Express"},{"key":"10.1016\/j.clinre.2019.04.003_bib0105","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1364\/BOE.6.001209","article-title":"Evaluation of area-based collagen scoring by nonlinear microscopy in chronic hepatitis C-induced liver fibrosis","volume":"6","author":"Sevrain","year":"2015","journal-title":"Biomed Opt Express"},{"key":"10.1016\/j.clinre.2019.04.003_bib0110","doi-asserted-by":"crossref","first-page":"1438","DOI":"10.1002\/hep.29009","article-title":"New classification of liver biopsy assessment for fibrosis in chronic hepatitis B patients before and after treatment","volume":"65","author":"Sun","year":"2017","journal-title":"Hepatology"},{"key":"10.1016\/j.clinre.2019.04.003_bib0115","doi-asserted-by":"crossref","first-page":"1891","DOI":"10.1002\/hep.29090","article-title":"Dual-photon microscopy-based quantitation of fibrosis-related parameters (q-FP) to model disease progression in steatohepatitis","volume":"65","author":"Wang","year":"2017","journal-title":"Hepatology"},{"key":"10.1016\/j.clinre.2019.04.003_bib0120","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1038\/labinvest.2016.128","article-title":"Automated evaluation of liver fibrosis in thioacetamide, carbon tetrachloride, and bile duct ligation rodent models using second-harmonic generation\/two-photon excited fluorescence microscopy","volume":"97","author":"Liu","year":"2017","journal-title":"Lab Invest"},{"key":"10.1016\/j.clinre.2019.04.003_bib0125","doi-asserted-by":"crossref","first-page":"064018","DOI":"10.1117\/1.3027970","article-title":"Second-harmonic generation and two-photon-excited autofluorescence microscopy of cardiomyocytes: quantification of cell volume and myosin filaments","volume":"13","author":"Wallace","year":"2008","journal-title":"J Biomed Opt"},{"key":"10.1016\/j.clinre.2019.04.003_bib0130","doi-asserted-by":"crossref","first-page":"9848","DOI":"10.1364\/AO.54.009848","article-title":"Analysis and quantification of collagen organization with the structure tensor in second harmonic microscopy images of ocular tissues","volume":"54","author":"Avila","year":"2015","journal-title":"Appl Opt"},{"key":"10.1016\/j.clinre.2019.04.003_bib0135","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1016\/j.kint.2016.06.030","article-title":"Label-free fluorescence lifetime and second harmonic generation imaging microscopy improves quantification of experimental renal fibrosis","volume":"90","author":"Ranjit","year":"2016","journal-title":"Kidney Int"},{"issue":"2","key":"10.1016\/j.clinre.2019.04.003_bib0140","doi-asserted-by":"crossref","first-page":"478A","DOI":"10.1016\/j.bpj.2014.11.2611","article-title":"Characterizing fibrosis in mouse kidney using auto fluorescence flim and SHG in UUO Model","volume":"108","author":"Levi","year":"2015","journal-title":"Biophys J"},{"key":"10.1016\/j.clinre.2019.04.003_bib0145","doi-asserted-by":"crossref","first-page":"086014","DOI":"10.1117\/1.JBO.19.8.086014","article-title":"Second harmonic generation microscopy analysis of extracellular matrix changes in human idiopathic pulmonary fibrosis","volume":"19","author":"Tilbury","year":"2014","journal-title":"J Biomed Opt"},{"key":"10.1016\/j.clinre.2019.04.003_bib0150","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/s12931-015-0220-8","article-title":"Second harmonic generation microscopy reveals altered collagen microstructure in usual interstitial pneumonia versus healthy lung","volume":"16","author":"Kottmann","year":"2015","journal-title":"Respir Res"},{"key":"10.1016\/j.clinre.2019.04.003_bib0155","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.jhep.2014.02.015","article-title":"qFibrosis: a fully-quantitative innovative method incorporating histological features to facilitate accurate fibrosis scoring in animal model and chronic hepatitis B patients","volume":"61","author":"Xu","year":"2014","journal-title":"J Hepatol"}],"container-title":["Clinics and Research in Hepatology and Gastroenterology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2210740119300932?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2210740119300932?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:28:21Z","timestamp":1761895701000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2210740119300932"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["S2210740119300932"],"URL":"https:\/\/doi.org\/10.1016\/j.clinre.2019.04.003","relation":{},"ISSN":["2210-7401"],"issn-type":[{"type":"print","value":"2210-7401"}],"subject":[],"published":{"date-parts":[[2020,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Improved quantitative assessment of HBV-associated liver fibrosis using second-harmonic generation microscopy with feature selection","name":"articletitle","label":"Article Title"},{"value":"Clinics and Research in Hepatology and Gastroenterology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.clinre.2019.04.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Masson SAS. All rights reserved.","name":"copyright","label":"Copyright"}]}}