{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T19:51:59Z","timestamp":1782417119129,"version":"3.54.5"},"reference-count":30,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalmicrobiologyandinfection.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Clinical Microbiology and Infection"],"published-print":{"date-parts":[[2016,10]]},"DOI":"10.1016\/j.cmi.2016.06.021","type":"journal-article","created":{"date-parts":[[2016,7,6]],"date-time":"2016-07-06T00:27:16Z","timestamp":1467764836000},"page":"862-868","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":98,"title":["Diagnosis of invasive fungal infections in haematological patients by combined use of galactomannan, 1,3-\u03b2-D-glucan, Aspergillus PCR, multifungal DNA-microarray, and Aspergillus azole resistance PCRs in blood and bronchoalveolar lavage samples: results of a prospective multicentre study"],"prefix":"10.1016","volume":"22","author":[{"given":"T.","family":"Boch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Spiess","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.A.","family":"Cornely","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.J.","family":"Vehreschild","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.M.","family":"Rath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Steinmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.J.","family":"Heinz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Hahn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.W.","family":"Krause","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.G.","family":"Kiehl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Egerer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Liebregts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Koldehoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Klein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Nolte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.C.","family":"Mueller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Merker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Will","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Mossner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Popp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.-K.","family":"Hofmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Reinwald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Buchheidt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cmi.2016.06.021_bib1","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1086\/651263","article-title":"Prospective surveillance for invasive fungal infections in hematopoietic stem cell transplant recipients, 2001\u20132006: overview of the transplant-associated infection surveillance network (transnet) database","volume":"50","author":"Kontoyiannis","year":"2010","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.cmi.2016.06.021_bib2","doi-asserted-by":"crossref","first-page":"e101","DOI":"10.1086\/521245","article-title":"The value of computed tomography-guided percutaneous lung biopsy for diagnosis of invasive fungal infection in immunocompromised patients","volume":"45","author":"Lass-Florl","year":"2007","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.cmi.2016.06.021_bib3","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1309\/AJCPO8VTSK3HRNUT","article-title":"Diagnostic accuracy of histopathologic and cytopathologic examination of aspergillus species","volume":"139","author":"Shah","year":"2013","journal-title":"Am J Clin Pathol"},{"key":"10.1016\/j.cmi.2016.06.021_bib4","doi-asserted-by":"crossref","first-page":"1760","DOI":"10.1128\/CVI.00226-08","article-title":"Performance characteristics of the platelia Aspergillus enzyme immunoassay for detection of aspergillus galactomannan antigen in bronchoalveolar lavage fluid","volume":"15","author":"Husain","year":"2008","journal-title":"Clin Vaccine Immun"},{"key":"10.1016\/j.cmi.2016.06.021_bib5","doi-asserted-by":"crossref","first-page":"E122","DOI":"10.1111\/j.1469-0691.2012.03777.x","article-title":"Post-diagnostic kinetics of the (1--> 3)-beta-d-glucan assay in invasive aspergillosis, invasive candidiasis and pneumocystis jirovecii pneumonia","volume":"18","author":"Koo","year":"2012","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.cmi.2016.06.021_bib6","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1086\/514349","article-title":"Optimization of the cutoff value for the aspergillus double-sandwich enzyme immunoassay","volume":"44","author":"Maertens","year":"2007","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.cmi.2016.06.021_bib7","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1128\/JCM.06423-11","article-title":"Detection of galactomannan in bronchoalveolar lavage fluid samples of patients at risk for invasive pulmonary aspergillosis: analytical and clinical validity","volume":"50","author":"D'Haese","year":"2012","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.cmi.2016.06.021_bib8","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1007\/s10096-014-2302-9","article-title":"How to interpret serum levels of beta-glucan for the diagnosis of invasive fungal infections in adult high-risk hematology patients: optimal cut-off levels and confounding factors","volume":"34","author":"Hammarstrom","year":"2015","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"10.1016\/j.cmi.2016.06.021_bib9","doi-asserted-by":"crossref","first-page":"3743","DOI":"10.1128\/JCM.00942-07","article-title":"DNA microarray-based detection and identification of fungal pathogens in clinical samples from neutropenic patients","volume":"45","author":"Spiess","year":"2007","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.cmi.2016.06.021_bib10","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1111\/myc.12424","article-title":"Identification of invasive fungal diseases in immunocompromised patients by combining an aspergillus specific pcr with a multifungal DNA-microarray from primary clinical samples","volume":"58","author":"Boch","year":"2015","journal-title":"Mycoses"},{"key":"10.1016\/j.cmi.2016.06.021_bib11","doi-asserted-by":"crossref","first-page":"3865","DOI":"10.1128\/JCM.37.12.3865-3871.1999","article-title":"Specific detection of Aspergillus species in blood and bronchoalveolar lavage samples of immunocompromised patients by two-step PCR","volume":"37","author":"Skladny","year":"1999","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.cmi.2016.06.021_bib12","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1111\/j.1600-0609.2012.01806.x","article-title":"Diagnosing pulmonary aspergillosis in patients with hematological malignancies: a multicenter prospective evaluation of an Aspergillus PCR assay and a galactomannan elisa in bronchoalveolar lavage samples","volume":"89","author":"Reinwald","year":"2012","journal-title":"Eur J Haematol"},{"key":"10.1016\/j.cmi.2016.06.021_bib13","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/S0732-8893(03)00168-8","article-title":"Diagnosing and monitoring of invasive aspergillosis during antifungal therapy by polymerase chain reaction: an experimental study in mice","volume":"47","author":"Lass-Fl\u00f6rl","year":"2003","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.cmi.2016.06.021_bib14","doi-asserted-by":"crossref","first-page":"2260","DOI":"10.1093\/jac\/dks208","article-title":"Therapy with antifungals decreases the diagnostic performance of pcr for diagnosing invasive aspergillosis in bronchoalveolar lavage samples of patients with haematological malignancies","volume":"67","author":"Reinwald","year":"2012","journal-title":"J\u00a0Antimicrob Chemother"},{"key":"10.1016\/j.cmi.2016.06.021_bib15","doi-asserted-by":"crossref","first-page":"124","DOI":"10.3109\/1040841X.2013.804033","article-title":"Utility of bronchoalveolar lavage fluid galactomannan alone or in combination with pcr for the diagnosis of invasive aspergillosis in adult hematology patients: a systematic review and meta-analysis","volume":"41","author":"Heng","year":"2015","journal-title":"Crit Rev Microbiol"},{"key":"10.1016\/j.cmi.2016.06.021_bib16","doi-asserted-by":"crossref","first-page":"3905","DOI":"10.1128\/AAC.05902-11","article-title":"Development of novel pcr assays to detect azole resistance-mediating mutations of the aspergillus fumigatus cyp51a gene in primary clinical samples from neutropenic patients","volume":"56","author":"Spiess","year":"2012","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.cmi.2016.06.021_bib17","doi-asserted-by":"crossref","first-page":"e103113","DOI":"10.1371\/journal.pone.0103113","article-title":"Incidence of Cyp51 a key mutations in Aspergillus fumigatus-a study on primary clinical samples of immunocompromised patients in the period of 1995\u20132013","volume":"9","author":"Spiess","year":"2014","journal-title":"PloS One"},{"key":"10.1016\/j.cmi.2016.06.021_bib18","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1086\/588660","volume":"46","author":"De Pauw","year":"2008","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.cmi.2016.06.021_bib19","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s10096-007-0437-7","article-title":"Diagnosis of invasive aspergillosis by galactomannan antigenemia detection using an enzyme immunoassay","volume":"27","author":"Wheat","year":"2008","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"10.1016\/j.cmi.2016.06.021_bib20","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1086\/421944","article-title":"Beta-d-glucan as a diagnostic adjunct for invasive fungal infections: Validation, cutoff development, and performance in patients with acute myelogenous leukemia and myelodysplastic syndrome","volume":"39","author":"Odabasi","year":"2004","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.cmi.2016.06.021_bib21","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1128\/JCM.00467-14","article-title":"Performance of galactomannan, beta-d-glucan, Aspergillus lateral-flow device, conventional culture, and PCR tests with bronchoalveolar lavage fluid for diagnosis of invasive pulmonary aspergillosis","volume":"52","author":"Hoenigl","year":"2014","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.cmi.2016.06.021_bib22","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1111\/j.1365-2141.2004.04904.x","volume":"125","author":"Buchheidt","year":"2004","journal-title":"Br J Haematol"},{"key":"10.1016\/j.cmi.2016.06.021_bib23","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1007\/s15010-013-0472-5","article-title":"Comparison of a novel aspergillus lateral-flow device and the platelia(r) galactomannan assay for the diagnosis of invasive aspergillosis following haematopoietic stem cell transplantation","volume":"41","author":"Held","year":"2013","journal-title":"Infection"},{"key":"10.1016\/j.cmi.2016.06.021_bib24","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.1128\/JCM.00110-15","article-title":"Aspergillus-specific lateral-flow device and real-time pcr testing of bronchoalveolar lavage fluid: a combination biomarker approach for clinical diagnosis of invasive pulmonary aspergillosis","volume":"53","author":"Johnson","year":"2015","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.cmi.2016.06.021_bib25","doi-asserted-by":"crossref","first-page":"1510","DOI":"10.1128\/JCM.03189-12","article-title":"Evaluation of real-time pcr, galactomannan enzyme-linked immunosorbent assay (elisa), and a novel lateral-flow device for diagnosis of invasive aspergillosis","volume":"51","author":"White","year":"2013","journal-title":"J\u00a0Clin Microbiol"},{"issue":"4","key":"10.1016\/j.cmi.2016.06.021_bib26","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1128\/JCM.03233-15","article-title":"A\u00a0comparison of the non-culture blood-based tests for diagnosing invasive aspergillosis in an animal model","volume":"54","author":"White","year":"2016","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.cmi.2016.06.021_bib27","doi-asserted-by":"crossref","first-page":"1688","DOI":"10.1086\/647935","article-title":"Bronchoalveolar lavage fluid galactomannan for the diagnosis of invasive pulmonary aspergillosis in patients with hematologic diseases","volume":"49","author":"Maertens","year":"2009","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.cmi.2016.06.021_bib28","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1111\/j.1439-0507.2011.02167.x","article-title":"Interlaboratory comparison of pcr-based identification of candida and aspergillus DNA in spiked blood samples","volume":"55","author":"Reichard","year":"2012","journal-title":"Mycoses"},{"issue":"8","key":"10.1016\/j.cmi.2016.06.021_bib29","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1093\/cid\/civ507","article-title":"Aspergillus PCR: A systematic review of evidence for clinical use in comparison with antigen testing","volume":"61","author":"White","year":"2015","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.cmi.2016.06.021_bib30","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.cmi.2015.09.009","article-title":"Prospective multicentre pcr-based aspergillus DNA screening in high-risk patients with and without primary antifungal mould prophylaxis","volume":"22","author":"Springer","year":"2016","journal-title":"Clin Microbiol Infect"}],"container-title":["Clinical Microbiology and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1198743X16302245?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1198743X16302245?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T07:20:19Z","timestamp":1761117619000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1198743X16302245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10]]},"references-count":30,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["S1198743X16302245"],"URL":"https:\/\/doi.org\/10.1016\/j.cmi.2016.06.021","relation":{},"ISSN":["1198-743X"],"issn-type":[{"value":"1198-743X","type":"print"}],"subject":[],"published":{"date-parts":[[2016,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Diagnosis of invasive fungal infections in haematological patients by combined use of galactomannan, 1,3-\u03b2-D-glucan, Aspergillus PCR, multifungal DNA-microarray, and Aspergillus azole resistance PCRs in blood and bronchoalveolar lavage samples: results of a prospective multicentre study","name":"articletitle","label":"Article Title"},{"value":"Clinical Microbiology and Infection","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cmi.2016.06.021","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}