{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:27:46Z","timestamp":1790710066424,"version":"4.1.0"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"crossref","award":["101-2221-E-006-100-MY3"],"award-info":[{"award-number":["101-2221-E-006-100-MY3"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Colloids and Surfaces B: Biointerfaces"],"published-print":{"date-parts":[[2017,2]]},"DOI":"10.1016\/j.colsurfb.2016.11.031","type":"journal-article","created":{"date-parts":[[2016,11,24]],"date-time":"2016-11-24T18:47:13Z","timestamp":1480013233000},"page":"98-105","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":22,"special_numbering":"C","title":["In-situ , time-lapse study of extracellular polymeric substance discharge in Streptococcus mutans biofilm"],"prefix":"10.1016","volume":"150","author":[{"given":"Bernard Haochih","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Chieh","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.colsurfb.2016.11.031_bib0005","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.1126\/science.283.5409.1837","article-title":"Forging a link between biofilms and disease","volume":"283","author":"Potera","year":"1999","journal-title":"Science"},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0010","doi-asserted-by":"crossref","first-page":"288","DOI":"10.3390\/pathogens2020288","article-title":"From in vitro to in vivo models of bacterial biofilm-related infections","volume":"2","author":"Lebeaux","year":"2013","journal-title":"Pathogens"},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0015","first-page":"141","article-title":"On the bacterial factor in the aetiology of dental caries","volume":"5","author":"Clarke","year":"1924","journal-title":"Br. J. Exp. Pathol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0020","doi-asserted-by":"crossref","first-page":"14434","DOI":"10.1073\/pnas.172501299","article-title":"Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen","volume":"99","author":"Ajdi\u0107","year":"2002","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0025","doi-asserted-by":"crossref","first-page":"2277","DOI":"10.1128\/IAI.00767-10","article-title":"The collagen-binding protein Cnm is required for Streptococcus mutans adherence to and intracellular invasion of human coronary artery endothelial cells","volume":"79","author":"Abranches","year":"2011","journal-title":"Infect. Immun."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0030","first-page":"141","article-title":"Invasion of human coronary artery endothelial cells by Streptococcus mutans OMZ175, Oral","volume":"24","author":"Abranches","year":"2009","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0035","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1159\/000057587","article-title":"Caries incidence in young type 1 diabetes mellitus patients in relation to metabolic control and caries-associated risk factors","volume":"36","author":"Twetman","year":"2002","journal-title":"Caries Res."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0040","doi-asserted-by":"crossref","first-page":"S314","DOI":"10.1093\/clinids\/16.Supplement_4.S314","article-title":"Bacterial colonization of saliva and plaque in the elderly","volume":"16","author":"Terpenning","year":"1993","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0045","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.colsurfb.2013.05.013","article-title":"Nanomechanical probing of the septum and surrounding substances on Streptococcus mutans cells and biofilms","volume":"110","author":"Liu","year":"2013","journal-title":"Colloids Surf. B. Biointerfaces"},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0050","doi-asserted-by":"crossref","first-page":"275401","DOI":"10.1088\/0022-3727\/46\/27\/275401","article-title":"In situ biosensing of the nanomechanical property and electrochemical spectroscopy of Streptococcus mutans-containing biofilms","volume":"46","author":"Liu","year":"2013","journal-title":"J. Phys. D: Appl. Phys."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0055","doi-asserted-by":"crossref","first-page":"4895","DOI":"10.1128\/IAI.72.8.4895-4899.2004","article-title":"Inactivation of the ciaH Gene in Streptococcus mutans diminishes mutacin production and competence development, alters sucrose-dependent biofilm formation, and reduces stress tolerance","volume":"72","author":"Qi","year":"2004","journal-title":"Infect. Immun."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0060","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1111\/j.1399-302X.1991.tb00453.x","article-title":"Adaptation by Streptococcus mutans to acid tolerance","volume":"6","author":"Hamilton","year":"1991","journal-title":"Oral Microbiol. Immunol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0065","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1016\/j.archoralbio.2008.04.005","article-title":"Electron microscopic detection and activity of glucosyltransferase B, C, and D in the in situ formed pellicle","volume":"53","author":"Hannig","year":"2008","journal-title":"Arch. Oral Biol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0070","doi-asserted-by":"crossref","first-page":"3024","DOI":"10.1128\/JB.01649-09","article-title":"Exopolysaccharides produced by Streptococcus mutans glucosyltransferases modulate the establishment of microcolonies within multispecies biofilms","volume":"192","author":"Koo","year":"2010","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0075","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1128\/JB.184.1.290-301.2002","article-title":"Biofilm formation and dispersal under the influence of the global regulator CsrA of Escherichia coli","volume":"184","author":"Jackson","year":"2002","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0080","doi-asserted-by":"crossref","first-page":"4457","DOI":"10.1016\/j.biomaterials.2003.11.031","article-title":"Evaluation of bacterial adhesion of Streptococcus mutans on dental restorative materials","volume":"25","author":"Montanaro","year":"2004","journal-title":"Biomaterials"},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0085","doi-asserted-by":"crossref","first-page":"e307","DOI":"10.1371\/journal.pbio.0050307","article-title":"Looking for chinks in the armor of bacterial biofilms","volume":"5","author":"Monroe","year":"2007","journal-title":"PLoS Biol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0090","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1146\/annurev.micro.54.1.49","article-title":"Biofilm formation as microbial development","volume":"54","author":"O'Toole","year":"2000","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0095","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1146\/annurev.mi.41.100187.002251","article-title":"Bacterial biofilms in nature and disease","volume":"41","author":"Costerton","year":"1987","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0100","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1139\/m77-249","article-title":"Microscopic examination of natural sessile bacterial populations from an alpine stream","volume":"23","author":"Geesey","year":"1977","journal-title":"Can. J. Microbiol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0105","series-title":"Microbial Extracellular Polymeric Substances","first-page":"1","article-title":"What are bacterial extracellular polymeric substances?","author":"Wingender","year":"1999"},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0110","article-title":"Microbial extracellular polymeric substances: characterization, structure and function","author":"Wingender","year":"2012","journal-title":"Springer Sci. Bus. Media"},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0115","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1146\/annurev.micro.55.1.165","article-title":"Quorum sensing in bacteria","volume":"55","author":"Miller","year":"2001","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0120","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1146\/annurev.cellbio.21.012704.131001","article-title":"Quorum sensing: cell-to-cell communication in bacteria","volume":"21","author":"Waters","year":"2005","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0125","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1128\/JB.25.3.277-287.1933","article-title":"Studies of freshwater bacteria: i. A direct microscopic technique","volume":"25","author":"Henrici","year":"1933","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0130","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1111\/j.1600-0722.2012.00983.x","article-title":"Influence of surface properties of resin\u2010based composites on in vitro Streptococcus mutans biofilm development","volume":"120","author":"Ionescu","year":"2012","journal-title":"Eur. J. Oral Sci."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0135","doi-asserted-by":"crossref","first-page":"4563","DOI":"10.1021\/la990805o","article-title":"Observation of changes in bacterial cell morphology using tapping mode atomic force microscopy","volume":"16","author":"Camesano","year":"2000","journal-title":"Langmuir"},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0140","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/S0304-3991(99)00139-4","article-title":"Atomic force microscopy imaging of living cells: a preliminary study of the disruptive effect of the cantilever tip on cell morphology","volume":"82","author":"You","year":"2000","journal-title":"Ultramicroscopy"},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0145","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1146\/annurev.physchem.52.1.71","article-title":"Surface biology of DNA by atomic force microscopy","volume":"52","author":"Hansma","year":"2001","journal-title":"Annu. Rev. Phys. Chem."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0150","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/BF01579285","article-title":"Atomic force microscopy examination of the topography of a hydrated bacterial biofilm on a copper surface","volume":"24","author":"Bremer","year":"1992","journal-title":"Curr. Microbiol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0155","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/0964-8305(94)90018-3","article-title":"An atomic force microscopy study of the biodeterioration of stainless steel in the presence of bacterial biofilms","volume":"34","author":"Steele","year":"1994","journal-title":"Int. Biodeterior. Biodegrad."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0160","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1002\/marc.201200359","article-title":"Quantifying the effect of covalently immobilized enzymes on biofilm formation by atomic force Microscopy\u2010Based Single\u2010Cell force spectroscopy","volume":"33","author":"Friedrichs","year":"2012","journal-title":"Macromol. Rapid Commun."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0165","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/j.resmic.2012.10.001","article-title":"Atomic force and super-resolution microscopy support a role for LapA as a cell-surface biofilm adhesin of Pseudomonas fluorescens","volume":"163","author":"Ivanov","year":"2012","journal-title":"Res. Microbiol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0170","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.1016\/S0006-3495(00)76724-5","article-title":"Atomic force and total internal reflection fluorescence microscopy for the study of force transmission in endothelial cells","volume":"78","author":"Mathur","year":"2000","journal-title":"Biophys. J."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0175","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1021\/nl0485399","article-title":"Nanoscale operation of a living cell using an atomic force microscope with a nanoneedle","volume":"5","author":"Obataya","year":"2005","journal-title":"Nano Lett."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0180","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.mechmat.2016.03.005","article-title":"Practical assessment of nanoscale indentation techniques for the biomechanical properties of biological materials","volume":"98","author":"Chang","year":"2016","journal-title":"Mech. Mater."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0185","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1111\/j.1472-765X.2007.02194.x","article-title":"Treatment of Streptococcus mutans biofilms with a nonthermal atmospheric plasma","volume":"45","author":"Sladek","year":"2007","journal-title":"Lett. Appl. Microbiol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0190","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/0021-9797(75)90018-1","article-title":"Effect of contact deformations on the adhesion of particles","volume":"53","author":"Derjaguin","year":"1975","journal-title":"J. Colloid Interface Sci."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0195","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1016\/j.bpj.2012.02.033","article-title":"Mass transfer enhancement in moving biofilm structures","volume":"102","author":"Taherzadeh","year":"2012","journal-title":"Biophys. J."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0200","series-title":"Community Structure and Co-operation in Biofilms","author":"Allison","year":"2000"},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0205","doi-asserted-by":"crossref","first-page":"098102","DOI":"10.1103\/PhysRevLett.93.098102","article-title":"Commonality of elastic relaxation times in biofilms","volume":"93","author":"Shaw","year":"2004","journal-title":"Phys. Rev. Lett."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0210","doi-asserted-by":"crossref","first-page":"2175","DOI":"10.1128\/AEM.71.4.2175-2178.2005","article-title":"Viscoelasticity of Staphylococcus aureus biofilms in response to fluid shear allows resistance to detachment and facilitates rolling migration","volume":"71","author":"Rupp","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0215","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S0045-6535(02)00319-3","article-title":"Biodegradability of biofilm extracellular polymeric substances","volume":"50","author":"Zhang","year":"2003","journal-title":"Chemosphere"},{"key":"10.1016\/j.colsurfb.2016.11.031_bib0220","doi-asserted-by":"crossref","first-page":"2355","DOI":"10.1128\/AEM.60.7.2355-2359.1994","article-title":"Role of alginate lyase in cell detachment of Pseudomonas aeruginosa","volume":"60","author":"Boyd","year":"1994","journal-title":"Appl. Environ. Microbiol."}],"container-title":["Colloids and Surfaces B: Biointerfaces"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0927776516308256?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0927776516308256?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,27]],"date-time":"2020-09-27T09:57:19Z","timestamp":1601200639000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0927776516308256"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2]]},"references-count":44,"alternative-id":["S0927776516308256"],"URL":"https:\/\/doi.org\/10.1016\/j.colsurfb.2016.11.031","relation":{},"ISSN":["0927-7765"],"issn-type":[{"value":"0927-7765","type":"print"}],"subject":[],"published":{"date-parts":[[2017,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"In-situ, time-lapse study of extracellular polymeric substance discharge in Streptococcus mutans biofilm","name":"articletitle","label":"Article Title"},{"value":"Colloids and Surfaces B: Biointerfaces","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.colsurfb.2016.11.031","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}