{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T02:37:46Z","timestamp":1792550266481,"version":"4.5.0"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Colloids and Surfaces B: Biointerfaces"],"published-print":{"date-parts":[[2022,8]]},"DOI":"10.1016\/j.colsurfb.2022.112566","type":"journal-article","created":{"date-parts":[[2022,5,13]],"date-time":"2022-05-13T11:46:21Z","timestamp":1652442381000},"page":"112566","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Formation of subvisible particles in commercial insulin formulations"],"prefix":"10.1016","volume":"216","author":[{"given":"Hamilton","family":"Silva-Jr","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Talita S.","family":"Ara\u00fajo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcius","family":"da Silva Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra M.N.","family":"Scapin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00eds Maur\u00edcio T.R.","family":"Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.colsurfb.2022.112566_bib1","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1073\/pnas.12.2.132","article-title":"Crystalline insulin","volume":"12","author":"Abel","year":"1926","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.colsurfb.2022.112566_bib2","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/S0021-9258(18)85482-5","article-title":"The preparation of insulin","volume":"57","author":"Best","year":"1923","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.colsurfb.2022.112566_bib3","series-title":"The Discovery of Insulin","author":"Bliss","year":"2000"},{"key":"10.1016\/j.colsurfb.2022.112566_bib4","series-title":"Galenics of Insulin","author":"Brange","year":"1987"},{"key":"10.1016\/j.colsurfb.2022.112566_bib5","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1177\/1932296817747292","article-title":"Insulin concentration in vials randomly purchased in pharmacies in the united states: considerable loss in the cold supply chain","volume":"12","author":"Carter","year":"2018","journal-title":"J. Diabetes Sci. Technol."},{"key":"10.1016\/j.colsurfb.2022.112566_bib6","first-page":"105","article-title":"Structure, aggregation, and activity of a covalent insulin dimer formed during storage of neutral formulation of human insulin","author":"Cf","year":"2016","journal-title":"J. Pharm. Sci."},{"key":"10.1016\/j.colsurfb.2022.112566_bib7","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1007\/BF00276849","article-title":"Insulin as an amyloid-fibril protein at sites of repeated insulin injections in a diabetic patient","volume":"31","author":"Dische","year":"1988","journal-title":"Diabetologia"},{"key":"10.1016\/j.colsurfb.2022.112566_bib8","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1016\/j.ejpb.2013.05.005","article-title":"Structural meta-analysis of regular human insulin in pharmaceutical formulations","volume":"85","author":"F\u00e1vero-Retto","year":"2013","journal-title":"Eur. J. Pharm. Biopharm.: Off. J. Arb. Fur Pharm. Verfahr. e. V."},{"key":"10.1016\/j.colsurfb.2022.112566_bib9","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.biologicals.2016.09.011","article-title":"Polymorphic distribution of proteins in solution by mass spectrometry: the analysis of insulin analogs","volume":"45","author":"F\u00e1vero-Retto","year":"2017","journal-title":"Biol.: J. Int. Assoc. Biol. Stand."},{"key":"10.1016\/j.colsurfb.2022.112566_bib10","series-title":"Bittersweet: Diabetes, Insulin, and the Transformation of Illness","author":"Feudtner","year":"2003"},{"key":"10.1016\/j.colsurfb.2022.112566_bib11","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1097\/NHH.0000000000000446","article-title":"A review of insulin for the treatment of diabetes mellitus","volume":"34","author":"Freeland","year":"2016","journal-title":"Home Healthc. Now."},{"key":"10.1016\/j.colsurfb.2022.112566_bib12","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1038\/nrd1695","article-title":"Protein drug stability: a formulation challenge","volume":"4","author":"Frokjaer","year":"2005","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.colsurfb.2022.112566_bib13","doi-asserted-by":"crossref","first-page":"2665","DOI":"10.2337\/diacare.26.9.2665","article-title":"How long should insulin be used once a vial is started?","volume":"26","author":"Grajower","year":"2003","journal-title":"Diabetes Care"},{"key":"10.1016\/j.colsurfb.2022.112566_bib14","doi-asserted-by":"crossref","first-page":"13246","DOI":"10.1073\/pnas.1005704107","article-title":"Supramolecular insulin assembly II for a sustained treatment of type 1 diabetes mellitus","volume":"107","author":"Gupta","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.colsurfb.2022.112566_bib15","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1177\/1932296819900258","article-title":"Insulin storage: a critical reappraisal","volume":"15","author":"Heinemann","year":"2020","journal-title":"J. Diabetes Sci. Technol."},{"key":"10.1016\/j.colsurfb.2022.112566_bib16","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1210\/endrev\/bnaa015","article-title":"The evolution of insulin and how it informs therapy and treatment choices","volume":"41","author":"Hirsch","year":"2020","journal-title":"Endocr. Rev."},{"key":"10.1016\/j.colsurfb.2022.112566_bib17","doi-asserted-by":"crossref","first-page":"2072","DOI":"10.1007\/s11095-014-1600-3","article-title":"Purification and identification of high molecular weight products formed during storage of neutral formulation of human insulin","volume":"32","author":"Hjorth","year":"2015","journal-title":"Pharm. Res."},{"key":"10.1016\/j.colsurfb.2022.112566_bib18","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0245372","article-title":"Heat-stability study of various insulin types in tropical temperature conditions: New insights towards improving diabetes care","volume":"16","author":"Kaufmann","year":"2021","journal-title":"PLoS One"},{"key":"10.1016\/j.colsurfb.2022.112566_bib19","doi-asserted-by":"crossref","first-page":"1595","DOI":"10.1177\/193229681300700620","article-title":"Stability and performance of rapid-acting insulin analogs used for continuous subcutaneous insulin infusion: a systematic review","volume":"7","author":"Kerr","year":"2013","journal-title":"J. Diabetes Sci. Technol."},{"key":"10.1016\/j.colsurfb.2022.112566_bib20","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.2337\/db18-1008-P","article-title":"The effect of temperature on the stability of in-use insulin pens","volume":"67","author":"Kongmalai","year":"2018","journal-title":"Diabetes"},{"key":"10.1016\/j.colsurfb.2022.112566_bib21","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/S0076-6879(99)09020-5","article-title":"Quantification of \u03b2-sheet amyloid fibril structures with thioflavin T","volume":"309","author":"LeVine","year":"1999","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.colsurfb.2022.112566_bib22","article-title":"Amyloidogenicity of peptides targeting diabetes and obesity","author":"Lima","year":"2021","journal-title":"Colloids Surf. B: Biointerfaces"},{"key":"10.1016\/j.colsurfb.2022.112566_bib23","doi-asserted-by":"crossref","unstructured":"L.M.T.R. Lima, T. Sisnande, Nutrient-sensing amyloid metastasis 2022 BioFactors, Oxford, England doi:\u00a010.1002\/biof.1825.","DOI":"10.1002\/biof.1825"},{"key":"10.1016\/j.colsurfb.2022.112566_bib24","doi-asserted-by":"crossref","first-page":"6036","DOI":"10.1021\/bi002555c","article-title":"Effect of environmental factors on the kinetics of insulin fibril formation: elucidation of the molecular mechanism","volume":"40","author":"Nielsen","year":"2001","journal-title":"Biochemistry"},{"key":"10.1016\/j.colsurfb.2022.112566_bib25","doi-asserted-by":"crossref","first-page":"6036","DOI":"10.1021\/bi002555c","article-title":"Effect of environmental factors on the kinetics of insulin fibril formation: elucidation of the molecular mechanism","volume":"40","author":"Nielsen","year":"2001","journal-title":"Biochemistry"},{"key":"10.1016\/j.colsurfb.2022.112566_bib26","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1080\/13506129.2016.1179183","article-title":"Insulin amyloid at injection sites of patients with diabetes","volume":"23","author":"Nilsson","year":"2016","journal-title":"Amyloid"},{"key":"10.1016\/j.colsurfb.2022.112566_bib27","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1177\/1932296820985845","article-title":"What do healthcare professionals and people with diabetes know about insulin transport and storage? A multinational survey","volume":"15","author":"Nitschke","year":"2021","journal-title":"J. Diabetes Sci. Technol."},{"key":"10.1016\/j.colsurfb.2022.112566_bib28","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0378-5173(96)04700-X","article-title":"Influence of temperature and shaking on stability of insulin preparations: degradation kinetics","volume":"143","author":"Oliva","year":"1996","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.colsurfb.2022.112566_bib29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bpc.2013.01.003","article-title":"A T3R3 hexamer of the human insulin variant B28Asp","volume":"173\u2013174","author":"Palmieri","year":"2013","journal-title":"Biophys. Chem."},{"key":"10.1016\/j.colsurfb.2022.112566_bib30","first-page":"130","article-title":"Effect of temperature on the potency & pharmacological action of insulin","author":"R","year":"2009","journal-title":"Indian J. Med. Res."},{"key":"10.1016\/j.colsurfb.2022.112566_bib31","doi-asserted-by":"crossref","unstructured":"D.C. Robbins, L. Andersen, R. Bowsher, R. Chance, B. Dinesen, B. Frank, R. Gingerich, D. Goldstein, H.M. Widemeyer, S. Haffner\u00a0et al., 1996. Report of the American Diabetes Association\u2019s Task. Force Stand. Insul. Assay. Diabetes, 45, pp. 242\u2013256.","DOI":"10.2337\/diabetes.45.2.242"},{"key":"10.1016\/j.colsurfb.2022.112566_bib32","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1089\/dia.2007.0233","article-title":"Comparison of in vitro stability for insulin aspart and insulin glulisine during simulated use in insulin pumps","volume":"9","author":"Senstius","year":"2007","journal-title":"Diabetes Technol. Ther."},{"key":"10.1016\/j.colsurfb.2022.112566_bib33","doi-asserted-by":"crossref","first-page":"9377","DOI":"10.1073\/pnas.88.21.9377","article-title":"Kinetics of insulin aggregation in aqueous solutions upon agitation in the presence of hydrophobic surfaces","volume":"88","author":"Sluzky","year":"1991","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.colsurfb.2022.112566_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.jpba.2021.114188","article-title":"Simultaneous characterization of insulin HMWP and protamine sulphate in complex formulations through SEC-coupled mass spectrometry","volume":"203","author":"Srivatsa","year":"2021","journal-title":"J. Pharm. Biomed. Anal."},{"key":"10.1016\/j.colsurfb.2022.112566_bib35","doi-asserted-by":"crossref","first-page":"499","DOI":"10.2337\/diab.17.8.499","article-title":"Effect of storage temperature on stability of commercial insulin preparations","volume":"17","author":"Storvick","year":"1968","journal-title":"Diabetes"},{"key":"10.1016\/j.colsurfb.2022.112566_bib36","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1093\/ajcn\/58.5.714","article-title":"Conversion of insulin units to SI units","volume":"58","author":"V\u00f8lund","year":"1993","journal-title":"Am. J. Clin. Nutr."},{"key":"10.1016\/j.colsurfb.2022.112566_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.tca.2020.178721","article-title":"Binding thermodynamics of divalent metal ions to several biological buffers","volume":"691","author":"Xiao","year":"2020","journal-title":"Thermochim. Acta"}],"container-title":["Colloids and Surfaces B: Biointerfaces"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0927776522002491?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0927776522002491?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T11:12:33Z","timestamp":1760526753000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0927776522002491"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8]]},"references-count":37,"alternative-id":["S0927776522002491"],"URL":"https:\/\/doi.org\/10.1016\/j.colsurfb.2022.112566","relation":{},"ISSN":["0927-7765"],"issn-type":[{"value":"0927-7765","type":"print"}],"subject":[],"published":{"date-parts":[[2022,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Formation of subvisible particles in commercial insulin formulations","name":"articletitle","label":"Article Title"},{"value":"Colloids and Surfaces B: Biointerfaces","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.colsurfb.2022.112566","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"112566"}}