{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T16:51:34Z","timestamp":1786467094642,"version":"build-2736575974"},"reference-count":98,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100013833","name":"I.M. Sechenov First Moscow State Medical University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013833","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["25-65-00010-P"],"award-info":[{"award-number":["25-65-00010-P"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Colloids and Surfaces B: Biointerfaces"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.colsurfb.2026.115956","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T16:25:42Z","timestamp":1783009542000},"page":"115956","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Safeguarding nanovesicles and their payload: A framework for stable storage"],"prefix":"10.1016","volume":"267","author":[{"given":"Artyom","family":"Kachanov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey","family":"Brezgin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasiya","family":"Kostyusheva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Ponomareva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Chulanov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Parodi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1851-7441","authenticated-orcid":false,"given":"Dmitry","family":"Kostyushev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"9","key":"10.1016\/j.colsurfb.2026.115956_bib1","article-title":"Engineering gene therapy: advances and barriers","volume":"4","author":"Shahryari","year":"2021","journal-title":"Adv. Ther."},{"issue":"3","key":"10.1016\/j.colsurfb.2026.115956_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s12276-019-0223-5","article-title":"Extracellular vesicle-based therapeutics: natural versus engineered targeting and trafficking","volume":"51","author":"Murphy","year":"2019","journal-title":"Exp. Mol. Med."},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib3","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1038\/s41392-024-01735-1","article-title":"Extracellular vesicles as tools and targets in therapy for diseases","volume":"9","author":"Kumar","year":"2024","journal-title":"Signal. Transduct. Target. Ther."},{"issue":"19","key":"10.1016\/j.colsurfb.2026.115956_bib4","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3390\/ijms251910401","article-title":"Basic Guide for Approaching Drug Delivery with Extracellular Vesicles","volume":"25","author":"Brezgin","year":"2024","journal-title":"Int. J. Mol. Sci."},{"issue":"10","key":"10.1016\/j.colsurfb.2026.115956_bib5","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.3390\/pharmaceutics16101306","article-title":"Biomimetic Nanoparticles for Basic Drug Delivery","volume":"16","author":"Tikhonov","year":"2024","journal-title":"Pharmaceutics"},{"key":"10.1016\/j.colsurfb.2026.115956_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.addr.2025.115522","article-title":"Breaking barriers in targeted Therapy: Advancing exosome Isolation, Engineering, and imaging","volume":"218","author":"Kostyusheva","year":"2025","journal-title":"Adv. Drug. Deliv. Rev."},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib7","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1038\/s41392-024-01735-1","article-title":"Extracellular vesicles as tools and targets in therapy for diseases","volume":"9","author":"Kumar","year":"2024","journal-title":"Signal. Transduct. Target. Ther."},{"issue":"2","key":"10.1016\/j.colsurfb.2026.115956_bib8","doi-asserted-by":"crossref","DOI":"10.1002\/btm2.10623","article-title":"Micro Trojan horses: Engineering extracellular vesicles crossing biological barriers for drug delivery","volume":"9","author":"Zeng","year":"2024","journal-title":"Bioeng. Transl. Med."},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/life15010070","article-title":"Extracellular Vesicles as Tools for Crossing the Blood\u2013Brain Barrier to Treat Lysosomal Storage Diseases","volume":"15","author":"Lerussi","year":"2025","journal-title":"Life"},{"key":"10.1016\/j.colsurfb.2026.115956_bib10","article-title":"Extracellular vesicles and the placenta\u2013brain axis: mechanisms of shared risk in pregnancy and perinatal psychiatry","volume":"47","author":"Hazzard","year":"2026","journal-title":"Curr. Opin. Physiol."},{"issue":"45","key":"10.1016\/j.colsurfb.2026.115956_bib11","doi-asserted-by":"crossref","first-page":"30943","DOI":"10.1021\/acsnano.3c12967","article-title":"Tumor-Derived Extracellular Vesicles Enable Tumor Tropism Chemo-Genetherapy for Local Immune Activation in Triple-Negative Breast Cancer","volume":"18","author":"Peng","year":"2024","journal-title":"ACS Nano"},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib12","article-title":"Isolation of Extracellular Vesicles: General Methodologies and Latest Trends","volume":"2018","author":"Konoshenko","year":"2018","journal-title":"BioMed. Res. Int."},{"key":"10.1016\/j.colsurfb.2026.115956_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.copbio.2024.103067","article-title":"Extracellular vesicles as therapeutics for inflammation and infection","volume":"85","author":"Levy","year":"2024","journal-title":"Curr. Opin. Biotechnol."},{"issue":"51","key":"10.1016\/j.colsurfb.2026.115956_bib14","doi-asserted-by":"crossref","first-page":"59199","DOI":"10.1021\/acsami.3c14377","article-title":"Anti-Inflammatory Artificial Extracellular Vesicles with Notable Inhibition of Particulate Matter-Induced Skin Inflammation and Barrier Function Impairment","volume":"15","author":"Park","year":"2023","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.colsurfb.2026.115956_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.jconrel.2025.113796","article-title":"Extracellular vesicle as therapeutic agents in anti-aging: Mechanistic insights and future potential","volume":"383","author":"Yoon","year":"2025","journal-title":"J. Control. Release"},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib16","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-023-39370-5","article-title":"Rejuvenating effects of young extracellular vesicles in aged rats and in cellular models of human senescence","volume":"13","author":"Grigorian Shamagian","year":"2023","journal-title":"Sci. Rep."},{"issue":"2","key":"10.1016\/j.colsurfb.2026.115956_bib17","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12404","article-title":"Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches","volume":"13","author":"Welsh","year":"2024","journal-title":"J. Extracell. Vesicles"},{"issue":"43","key":"10.1016\/j.colsurfb.2026.115956_bib18","doi-asserted-by":"crossref","first-page":"17432","DOI":"10.1021\/acs.analchem.4c04607","article-title":"Saponin is Essential for the Isolation of Proteins and RNA from Biological Nanoparticles","volume":"96","author":"Brezgin","year":"2024","journal-title":"Anal. Chem."},{"key":"10.1016\/j.colsurfb.2026.115956_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.biotechadv.2023.108122","article-title":"Technological aspects of manufacturing and analytical control of biological nanoparticles","volume":"64","author":"Brezgin","year":"2023","journal-title":"Biotechnol. Adv."},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib20","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1186\/s13287-024-04005-7","article-title":"Different storage and freezing protocols for extracellular vesicles: a systematic review","volume":"15","author":"Ahmadian","year":"2024","journal-title":"Stem Cell. Res. Ther."},{"issue":"4","key":"10.1016\/j.colsurfb.2026.115956_bib21","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1093\/ilar.41.4.187","article-title":"Mechanisms of Cryoinjury in Living Cells","volume":"41","author":"Gao","year":"2000","journal-title":"ILAR J."},{"issue":"8","key":"10.1016\/j.colsurfb.2026.115956_bib22","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1038\/s41570-022-00407-4","article-title":"Chemical approaches to cryopreservation","volume":"6","author":"Murray","year":"2022","journal-title":"Nat. Rev. Chem."},{"key":"10.1016\/j.colsurfb.2026.115956_bib23","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.1016\/S0022-0248(01)02271-0","article-title":"Relationship between growth rate and supercooling in the formation of ice lenses in a glass powder","volume":"237-239","author":"Watanabe","year":"2002","journal-title":"J. Cryst. Growth"},{"issue":"5","key":"10.1016\/j.colsurfb.2026.115956_bib24","doi-asserted-by":"crossref","first-page":"e0251290","DOI":"10.1371\/journal.pone.0251290","article-title":"Isolation of intact extracellular vesicles from cryopreserved samples","volume":"16","author":"Tessier","year":"2021","journal-title":"PloS. One"},{"issue":"11","key":"10.1016\/j.colsurfb.2026.115956_bib25","doi-asserted-by":"crossref","first-page":"7663","DOI":"10.1002\/jcp.29700","article-title":"How does temperature play a role in the storage of extracellular vesicles?","volume":"235","author":"Qin","year":"2020","journal-title":"J. Cell. Physiol."},{"key":"10.1016\/j.colsurfb.2026.115956_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijpharm.2020.119551","article-title":"Effect of particle size on the pharmacokinetics and biodistribution of parenteral nanoemulsions","volume":"586","author":"Fan","year":"2020","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.colsurfb.2026.115956_bib27","doi-asserted-by":"crossref","unstructured":"BT Ledford, TG Wyatt, J Vang, J Weiss, ND Tsihlis, MR. KibbeEffects of Particle Size, Charge, Shape, Animal Disease State, and Sex on the Biodistribution of Intravenously Administered Nanoparticles. doi:10.1002\/ppsc.202300001.","DOI":"10.1002\/ppsc.202300001"},{"issue":"11","key":"10.1016\/j.colsurfb.2026.115956_bib28","doi-asserted-by":"crossref","first-page":"7663","DOI":"10.1002\/jcp.29700","article-title":"How does temperature play a role in the storage of extracellular vesicles?","volume":"235","author":"Qin","year":"2020","journal-title":"J. Cell. Physiol."},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib29","doi-asserted-by":"crossref","DOI":"10.1080\/20013078.2017.1359478","article-title":"Effects of storage temperature on airway exosome integrity for diagnostic and functional analyses","volume":"6","author":"Maroto","year":"2017","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.colsurfb.2026.115956_bib30","doi-asserted-by":"crossref","DOI":"10.3402\/jev.v3.25465","article-title":"Effect of storage on physical and functional properties of extracellular vesicles derived from neutrophilic granulocytes","volume":"3","author":"L\u0151rincz","year":"2014","journal-title":"J. Extracell. Vesicles"},{"issue":"147","key":"10.1016\/j.colsurfb.2026.115956_bib31","first-page":"59584","article-title":"Evaluation of the Storage Stability of Extracellular Vesicles","author":"Richter","year":"2019","journal-title":"J. Vis. Exp."},{"issue":"4","key":"10.1016\/j.colsurfb.2026.115956_bib32","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/s13238-018-0529-4","article-title":"Effect of pH, temperature and freezing-thawing on quantity changes and cellular uptake of exosomes","volume":"10","author":"Cheng","year":"2019","journal-title":"Protein Cell."},{"key":"10.1016\/j.colsurfb.2026.115956_bib33","first-page":"6675","article-title":"Effects of Cryopreservation on Microparticles Concentration, Procoagulant Function, Size Distribution, and Morphology","volume":"25","author":"Dong","year":"2019","journal-title":"Med. Sci. Monit. Int. Med. J. Exp. Clin. Res."},{"issue":"2","key":"10.1016\/j.colsurfb.2026.115956_bib34","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2020241118","article-title":"Engineering exosome polymer hybrids by atom transfer radical polymerization","volume":"118","author":"Lathwal","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib35","doi-asserted-by":"crossref","DOI":"10.1038\/srep36162","article-title":"Trehalose prevents aggregation of exosomes and cryodamage","volume":"6","author":"Bosch","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.colsurfb.2026.115956_bib36","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.1016\/j.proeng.2012.07.564","article-title":"Stability and Rheology of Aqueous Suspensions","volume":"42","author":"Ve\u010de\u0159","year":"2012","journal-title":"Procedia Eng."},{"issue":"27","key":"10.1016\/j.colsurfb.2026.115956_bib37","doi-asserted-by":"crossref","first-page":"16701","DOI":"10.1021\/acsomega.0c01582","article-title":"Zeta Potential of Extracellular Vesicles: Toward Understanding the Attributes that Determine Colloidal Stability","volume":"5","author":"Midekessa","year":"2020","journal-title":"ACS Omega"},{"issue":"5","key":"10.1016\/j.colsurfb.2026.115956_bib38","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.202100538","article-title":"Enhancing the Stabilization Potential of Lyophilization for Extracellular Vesicles","volume":"11","author":"Trenkenschuh","year":"2022","journal-title":"Adv. Healthc. Mater."},{"key":"10.1016\/j.colsurfb.2026.115956_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijpharm.2023.123211","article-title":"Investigating freezing-induced acidity changes in citrate buffers","volume":"643","author":"Susrisweta","year":"2023","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.colsurfb.2026.115956_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/S0378-5173(00)00423-3","article-title":"Lyophilization and development of solid protein pharmaceuticals","author":"Wang","year":"2000","journal-title":"Int. J. Pharm."},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib41","doi-asserted-by":"crossref","DOI":"10.3402\/jev.v4.29509","article-title":"High-speed centrifugation induces aggregation of extracellular vesicles","volume":"4","author":"Linares","year":"2015","journal-title":"J. Extracell. Vesicles"},{"issue":"5","key":"10.1016\/j.colsurfb.2026.115956_bib42","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.1007\/s10616-016-9952-7","article-title":"Evaluation of electroporation-induced adverse effects on adipose-derived stem cell exosomes","volume":"68","author":"Johnsen","year":"2016","journal-title":"Cytotechnology"},{"key":"10.1016\/j.colsurfb.2026.115956_bib43","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.ab.2013.12.001","article-title":"Maximizing Exosome Colloidal Stability Following Electroporation","volume":"448","author":"Hood","year":"2014","journal-title":"Anal. Biochem."},{"issue":"3","key":"10.1016\/j.colsurfb.2026.115956_bib44","doi-asserted-by":"crossref","DOI":"10.1007\/s11060-013-1235-y","article-title":"Heparin blocks transfer of extracellular vesicles between donor and recipient cells","volume":"115","author":"Atai","year":"2013","journal-title":"J. Neurooncol"},{"issue":"12","key":"10.1016\/j.colsurfb.2026.115956_bib45","doi-asserted-by":"crossref","first-page":"e0243738","DOI":"10.1371\/journal.pone.0243738","article-title":"Adsorption of extracellular vesicles onto the tube walls during storage in solution","volume":"15","author":"Evtushenko","year":"2020","journal-title":"PLoS. ONE"},{"key":"10.1016\/j.colsurfb.2026.115956_bib46","doi-asserted-by":"crossref","unstructured":"A G\u00f6rgens, G Corso, DW Hagey, Identification of storage conditions stabilizing extracellular vesicles preparations. doi:10.1002\/jev2.12238.","DOI":"10.1002\/jev2.12238"},{"key":"10.1016\/j.colsurfb.2026.115956_bib47","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2019.01293","article-title":"Unpredictable Nanoparticle Retention in Commonly Used Plastic Syringes Introduces Dosage Uncertainties That May Compromise the Accuracy of Nanomedicine and Nanotoxicology Studies","volume":"10","author":"Holzwarth","year":"2019","journal-title":"Front. Pharmacol."},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib48","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1038\/s41392-024-01735-1","article-title":"Extracellular vesicles as tools and targets in therapy for diseases","volume":"9","author":"Kumar","year":"2024","journal-title":"Signal. Transduct. Target. Ther."},{"issue":"2","key":"10.1016\/j.colsurfb.2026.115956_bib49","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1089\/bio.2022.0007","article-title":"Effects of Freezing and Rewarming Methods on RNA Quality of Blood Samples","volume":"21","author":"Zou","year":"2023","journal-title":"Biopreservation Biobanking"},{"issue":"5","key":"10.1016\/j.colsurfb.2026.115956_bib50","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1080\/10837450701481157","article-title":"Protein stability during freezing: separation of stresses and mechanisms of protein stabilization","volume":"12","author":"Bhatnagar","year":"2007","journal-title":"Pharm. Dev. Technol."},{"issue":"10","key":"10.1016\/j.colsurfb.2026.115956_bib51","doi-asserted-by":"crossref","first-page":"5871","DOI":"10.1021\/acsbiomaterials.3c00678","article-title":"Comparative Studies of Different Preservation Methods and Relative Freeze-Drying Formulations for Extracellular Vesicle Pharmaceutical Applications","volume":"9","author":"Susa","year":"2023","journal-title":"ACS Biomater. Sci. Eng."},{"issue":"6","key":"10.1016\/j.colsurfb.2026.115956_bib52","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1016\/j.ymthe.2017.03.030","article-title":"Engineered Exosomes as Vehicles for Biologically Active Proteins","volume":"25","author":"Sterzenbach","year":"2017","journal-title":"Mol. Ther. J. Am. Soc. Gene Ther."},{"issue":"2","key":"10.1016\/j.colsurfb.2026.115956_bib53","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1007\/s12257-015-0781-x","article-title":"Influence of storage condition on exosome recovery","volume":"21","author":"Lee","year":"2016","journal-title":"Biotechnol. Bioprocess. Eng."},{"issue":"5","key":"10.1016\/j.colsurfb.2026.115956_bib54","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.jcyt.2022.11.006","article-title":"The Impact of Storage Condition and Duration on Function of Native and Cargo-Loaded Mesenchymal Stromal Cell Extracellular Vesicles","volume":"25","author":"Levy","year":"2023","journal-title":"Cytotherapy"},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib55","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.colsurfb.2011.05.013","article-title":"Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy","volume":"87","author":"Sokolova","year":"2011","journal-title":"Colloids Surf. B Biointerfaces"},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib56","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1208\/s12248-022-00767-1","article-title":"Stability and Function of Extracellular Vesicles Derived from Immortalized Human Corneal Stromal Stem Cells: A Proof of Concept Study","volume":"25","author":"Lyu","year":"2022","journal-title":"AAPS J."},{"key":"10.1016\/j.colsurfb.2026.115956_bib57","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2019.119675","article-title":"Post-production modifications of murine mesenchymal stem cell (mMSC) derived extracellular vesicles (EVs) and impact on their cellular interaction","volume":"231","author":"Le Saux","year":"2020","journal-title":"Biomaterials"},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib58","doi-asserted-by":"crossref","DOI":"10.1080\/20013078.2017.1359478","article-title":"Effects of storage temperature on airway exosome integrity for diagnostic and functional analyses","volume":"6","author":"Maroto","year":"2017","journal-title":"J. Extracell. Vesicles"},{"issue":"2","key":"10.1016\/j.colsurfb.2026.115956_bib59","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1007\/s12257-015-0781-x","article-title":"Influence of storage condition on exosome recovery","volume":"21","author":"Lee","year":"2016","journal-title":"Biotechnol. Bioprocess. Eng."},{"issue":"4","key":"10.1016\/j.colsurfb.2026.115956_bib60","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/0011-2240(78)90056-1","article-title":"Differing actions of penetrating and nonpenetrating cryoprotective agents","volume":"15","author":"McGann","year":"1978","journal-title":"Cryobiology"},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib61","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/23144599.2019.1704992","article-title":"Preservation techniques of stem cells extracellular vesicles: a gate for manufacturing of clinical grade therapeutic extracellular vesicles and long-term clinical trials","volume":"8","author":"Bahr","year":"2020","journal-title":"Int. J. Vet. Sci. Med."},{"key":"10.1016\/j.colsurfb.2026.115956_bib62","doi-asserted-by":"crossref","unstructured":"S Gelibter, G Marostica, A Mandelli, The impact of storage on extracellular vesicles: A systematic study. doi:10.1002\/jev2.12162.","DOI":"10.1002\/jev2.12162"},{"key":"10.1016\/j.colsurfb.2026.115956_bib63","article-title":"Preservation of extracellular vesicles for drug delivery: A comparative evaluation of storage buffers","volume":"107","author":"Geng","year":"2025","journal-title":"J. Drug. Deliv. Sci. Technol."},{"key":"10.1016\/j.colsurfb.2026.115956_bib64","doi-asserted-by":"crossref","DOI":"10.1016\/j.vesic.2022.100016","article-title":"Sucrose-based cryoprotective storage of extracellular vesicles","volume":"1","author":"Walker","year":"2022","journal-title":"Extracell. Vesicle"},{"issue":"4","key":"10.1016\/j.colsurfb.2026.115956_bib65","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/0011-2240(84)90079-8","article-title":"Vitrification as an approach to cryopreservation","volume":"21","author":"Fahy","year":"1984","journal-title":"Cryobiology"},{"issue":"2","key":"10.1016\/j.colsurfb.2026.115956_bib66","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.fertnstert.2019.12.009","article-title":"Vitrification of the human embryo: a more efficient and safer in vitro fertilization treatment","volume":"113","author":"Nagy","year":"2020","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.colsurfb.2026.115956_bib67","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/978-1-4939-2193-5_2","article-title":"Principles of cryopreservation by vitrification","volume":"1257","author":"Fahy","year":"2015","journal-title":"Methods Mol. Biol."},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib68","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1186\/s13148-022-01355-y","article-title":"Advantages of vitrification preservation in assisted reproduction and potential influences on imprinted genes","volume":"14","author":"Chen","year":"2022","journal-title":"Clin. Epigenetics"},{"key":"10.1016\/j.colsurfb.2026.115956_bib69","article-title":"Lyophilization of pharmaceuticals: an overview and practical approaches for developing the lyophilization cycle","author":"Lee","year":"2025","journal-title":"J. Pharm. Investig."},{"key":"10.1016\/j.colsurfb.2026.115956_bib70","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.jddst.2017.03.013","article-title":"Effects of temperature ramp rate during the primary drying process on the properties of amorphous-based lyophilized cake, Part 1: Cake characterization, collapse temperature and drying behavior","volume":"39","author":"Ohori","year":"2017","journal-title":"J. Drug. Deliv. Sci. Technol."},{"issue":"2","key":"10.1016\/j.colsurfb.2026.115956_bib71","doi-asserted-by":"crossref","first-page":"275","DOI":"10.3390\/biomedicines13020275","article-title":"Lyophilized Small Extracellular Vesicles (sEVs) Derived from Human Adipose Stem Cells Maintain Efficacy to Promote Healing in Neuronal Injuries","volume":"13","author":"Jones","year":"2025","journal-title":"Biomedicines"},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib72","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s13036-024-00470-z","article-title":"Stabilizing milk-derived extracellular vesicles (mEVs) through lyophilization: a novel trehalose and tryptophan formulation for maintaining structure and Bioactivity during long-term storage","volume":"19","author":"Dogan","year":"2025","journal-title":"J. Biol. Eng."},{"key":"10.1016\/j.colsurfb.2026.115956_bib73","doi-asserted-by":"crossref","DOI":"10.34133\/bmr.0005","article-title":"Lyoprotectant Constituents Suited for Lyophilization and Reconstitution of Stem-Cell-Derived Extracellular Vesicles","volume":"28","author":"Kang","year":"2024","journal-title":"Biomater. Res."},{"issue":"10","key":"10.1016\/j.colsurfb.2026.115956_bib74","doi-asserted-by":"crossref","first-page":"5871","DOI":"10.1021\/acsbiomaterials.3c00678","article-title":"Comparative Studies of Different Preservation Methods and Relative Freeze-Drying Formulations for Extracellular Vesicle Pharmaceutical Applications","volume":"9","author":"Susa","year":"2023","journal-title":"ACS Biomater. Sci. Eng."},{"key":"10.1016\/j.colsurfb.2026.115956_bib75","doi-asserted-by":"crossref","DOI":"10.1016\/S0169-409X(00)00144-7","article-title":"Factors affecting short-term and long-term stabilities of proteins","author":"Arakawa","year":"2001","journal-title":"Adv. Drug. Deliv. Rev."},{"issue":"2","key":"10.1016\/j.colsurfb.2026.115956_bib76","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1006\/abbi.1999.1175","article-title":"Hydrogen bonding between sugar and protein is responsible for inhibition of dehydration-induced protein unfolding","volume":"365","author":"Allison","year":"1999","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1016\/j.colsurfb.2026.115956_bib77","article-title":"Clarifying the role of cryo- and lyo-protectants in the biopreservation of proteins","author":"Pisano","year":"2018","journal-title":"Phys. Chem. Chem. Phys."},{"issue":"16","key":"10.1016\/j.colsurfb.2026.115956_bib78","doi-asserted-by":"crossref","first-page":"6301","DOI":"10.7150\/thno.98671","article-title":"Preclinical pharmacology of patient-derived extracellular vesicles for the intraoperative imaging of tumors","volume":"14","author":"Villa","year":"2024","journal-title":"Theranostics"},{"issue":"6","key":"10.1016\/j.colsurfb.2026.115956_bib79","doi-asserted-by":"crossref","first-page":"2931","DOI":"10.1016\/j.ymthe.2025.02.033","article-title":"Lyophilized apoptotic vesicles improve hemostasis and bone regeneration in traumatic patients with impacted third molar extraction","volume":"33","author":"Jiang","year":"2025","journal-title":"Mol. Ther. J. Am. Soc. Gene Ther."},{"key":"10.1016\/j.colsurfb.2026.115956_bib80","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1186\/s12951-023-02128-2","article-title":"Lyophilized apoptotic vesicle-encapsulated adhesive hydrogel sponge as a rapid hemostat for traumatic hemorrhage in coagulopathy","volume":"21","author":"Jiang","year":"2023","journal-title":"J. Nanobiotechnology"},{"key":"10.1016\/j.colsurfb.2026.115956_bib81","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijpharm.2024.124576","article-title":"Functional dry powders of connexin-containing extracellular vesicles","volume":"663","author":"Hanafy","year":"2024","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.colsurfb.2026.115956_bib82","doi-asserted-by":"crossref","first-page":"181","DOI":"10.3389\/fcell.2020.00181","article-title":"Freeze-Dried Extracellular Vesicles From Adipose-Derived Stem Cells Prevent Hypoxia-Induced Muscle Cell Injury","volume":"8","author":"El Baradie","year":"2020","journal-title":"Front. Cell. Dev. Biol."},{"issue":"5","key":"10.1016\/j.colsurfb.2026.115956_bib83","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.3390\/biomedicines13051069","article-title":"Biomanufacturing and Curcumin-Loading of Human Choroid Plexus Organoid-Derived Extracellular Vesicles from a Vertical-Wheel Bioreactor to Alleviate Neuro-Inflammation","volume":"13","author":"Ene","year":"2025","journal-title":"Biomedicines"},{"issue":"10","key":"10.1016\/j.colsurfb.2026.115956_bib84","doi-asserted-by":"crossref","first-page":"1154","DOI":"10.3390\/vaccines9101154","article-title":"A Four-Step Purification Process for Gag VLPs: From Culture Supernatant to High-Purity Lyophilized Particles","volume":"9","author":"Gonz\u00e1lez-Dom\u00ednguez","year":"2021","journal-title":"Vaccines"},{"key":"10.1016\/j.colsurfb.2026.115956_bib85","doi-asserted-by":"crossref","DOI":"10.1016\/j.colsurfb.2022.112745","article-title":"Efficient extracellular vesicles freeze-dry method for direct formulations preparation and use","volume":"218","author":"Guarro","year":"2022","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"10.1016\/j.colsurfb.2026.115956_bib86","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2024.1452428","article-title":"Functionality of lyophilized osteoinductive EVs: a mechanistic study","volume":"12","author":"Huang","year":"2024","journal-title":"Front. Bioeng. Biotechnol."},{"issue":"5","key":"10.1016\/j.colsurfb.2026.115956_bib87","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12058","article-title":"Extracellular vesicles from recombinant cell factories improve the activity and efficacy of enzymes defective in lysosomal storage disorders","volume":"10","author":"Seras-Franzoso","year":"2021","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.colsurfb.2026.115956_bib88","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-30786-y","article-title":"Extracellular vesicles protect glucuronidase model enzymes during freeze-drying","volume":"8","author":"Frank","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.colsurfb.2026.115956_bib89","doi-asserted-by":"crossref","DOI":"10.1021\/acsbiomaterials.5c00760","article-title":"Harnessing Microneedles for Delivery and Preservation of Natural Killer Cell-Derived Extracellular Vesicles","author":"Lim","year":"2025","journal-title":"ACS Biomater. Sci. Eng."},{"key":"10.1016\/j.colsurfb.2026.115956_bib90","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2022.121644","article-title":"Dissolving microneedles for long-term storage and transdermal delivery of extracellular vesicles","volume":"287","author":"Bui","year":"2022","journal-title":"Biomaterials"},{"key":"10.1016\/j.colsurfb.2026.115956_bib91","doi-asserted-by":"crossref","unstructured":"C Wang, A Fulati, K Kimura, Encapsulation of Small Extracellular Vesicles into Selectively Disassemblable Shells of PEGylated Metal-Phenolic Networks. Adv Healthc Mater. n\/a(n\/a):2405188. doi:10.1002\/adhm.202405188.","DOI":"10.1002\/adhm.202405188"},{"issue":"18","key":"10.1016\/j.colsurfb.2026.115956_bib92","doi-asserted-by":"crossref","DOI":"10.1002\/smll.202412264","article-title":"A Biomimetic Mineralization Strategy for the Long-Term Preservation of Exosomes Through Non-Destructive Encapsulation Within Zeolite Imidazolate Frameworks-8","volume":"21","author":"Jia","year":"2025","journal-title":"Small"},{"key":"10.1016\/j.colsurfb.2026.115956_bib93","doi-asserted-by":"crossref","first-page":"7831","DOI":"10.2147\/IJN.S334194","article-title":"Preservation of Small Extracellular Vesicle in Gelatin Methacryloyl Hydrogel Through Reduced Particles Aggregation for Therapeutic Applications","volume":"16","author":"Wu","year":"2021","journal-title":"Int. J. Nanomed."},{"issue":"2","key":"10.1016\/j.colsurfb.2026.115956_bib94","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1002\/bit.70105","article-title":"Comparative Analysis of HEK293 Genomic Variability","volume":"123","author":"Smesnik","year":"2026","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.colsurfb.2026.115956_bib95","doi-asserted-by":"crossref","DOI":"10.1016\/j.tiv.2021.105178","article-title":"Inter-laboratory variability of A549 epithelial cells grown under submerged and air-liquid interface conditions","volume":"75","author":"Barosova","year":"2021","journal-title":"Toxicol. Vitr."},{"key":"10.1016\/j.colsurfb.2026.115956_bib96","article-title":"DONOR VARIABILITY IN HUMAN MESENCHYMAL STEM CELL OSTEOGENIC RESPONSE AS A FUNCTION OF PASSAGE CONDITIONS AND DONOR SEX","volume":"15","author":"Kolliopoulos","year":"2023","journal-title":"bioRxiv Prepr. Serv. Biol."},{"issue":"1","key":"10.1016\/j.colsurfb.2026.115956_bib97","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-024-62544-8","article-title":"Advantages of pooling of human bone marrow-derived mesenchymal stromal cells from different donors versus single-donor MSCs","volume":"14","author":"Kannan","year":"2024","journal-title":"Sci. Rep."},{"issue":"2","key":"10.1016\/j.colsurfb.2026.115956_bib98","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12404","article-title":"Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches","volume":"13","author":"Welsh","year":"2024","journal-title":"J. Extracell. Vesicles"}],"container-title":["Colloids and Surfaces B: Biointerfaces"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0927776526005448?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0927776526005448?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T16:04:23Z","timestamp":1786464263000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0927776526005448"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":98,"alternative-id":["S0927776526005448"],"URL":"https:\/\/doi.org\/10.1016\/j.colsurfb.2026.115956","relation":{},"ISSN":["0927-7765"],"issn-type":[{"value":"0927-7765","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Safeguarding nanovesicles and their payload: A framework for stable storage","name":"articletitle","label":"Article Title"},{"value":"Colloids and Surfaces B: Biointerfaces","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.colsurfb.2026.115956","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"115956"}}