{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T00:28:21Z","timestamp":1793924901575,"version":"4.8.0"},"reference-count":100,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82172745"],"award-info":[{"award-number":["82172745"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004300","name":"Senior Talent Foundation of Jiangsu University","doi-asserted-by":"publisher","award":["20JDG064"],"award-info":[{"award-number":["20JDG064"]}],"id":[{"id":"10.13039\/501100004300","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Colloids and Surfaces B: Biointerfaces"],"published-print":{"date-parts":[[2027,1]]},"DOI":"10.1016\/j.colsurfb.2026.116165","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T15:03:54Z","timestamp":1788879834000},"page":"116165","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Programmable crosslinking strategies for the rational design of engineered protein hydrogels"],"prefix":"10.1016","volume":"269","author":[{"given":"Tingyu","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haonan","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1390-2460","authenticated-orcid":false,"given":"Shengwang","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.colsurfb.2026.116165_bib1","doi-asserted-by":"crossref","first-page":"7416","DOI":"10.1021\/jacs.7b00513","article-title":"Designing peptide and protein modified hydrogels: selecting the optimal conjugation strategy","volume":"139","author":"Fisher","year":"2017","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.colsurfb.2026.116165_bib2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1038\/s41467-023-44564-6","article-title":"Programmable adhesion and morphing of protein hydrogels for underwater robots","volume":"15","author":"Huang","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.colsurfb.2026.116165_bib3","doi-asserted-by":"crossref","DOI":"10.1002\/adfm.202514002","article-title":"Multifunctional protein eutectogels based on polyphenolic: zwitterionic low-transition-temperature mixtures","volume":"36","author":"Martin-Salda\u00f1a","year":"2026","journal-title":"Adv. Funct. Mater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib4","article-title":"Recent advances in protein hydrogels: from design, structural and functional regulations to healthcare applications","volume":"451","author":"Zhang","year":"2023","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.colsurfb.2026.116165_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2024.148881","article-title":"Controlling spatiotemporal mechanics of globular protein-polymer hydrogel via metal-coordination interactions","volume":"482","author":"Dutta","year":"2024","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.colsurfb.2026.116165_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodchem.2025.143175","article-title":"Fabrication of CMC-PVA-EGDE hydrogels for maintaining overall postharvest quality of small-size packaged straw mushroom","volume":"474","author":"Jin","year":"2025","journal-title":"Food Chem."},{"key":"10.1016\/j.colsurfb.2026.116165_bib7","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1021\/acsmacrolett.6b00750","article-title":"Protein-cross-linked triple-responsive polymer networks based on molecular recognition","volume":"5","author":"Chen","year":"2016","journal-title":"ACS Macro Lett."},{"key":"10.1016\/j.colsurfb.2026.116165_bib8","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1039\/C8BM01246F","article-title":"Advances in crosslinking strategies of biomedical hydrogels","volume":"7","author":"Hu","year":"2019","journal-title":"Biomater. Sci."},{"key":"10.1016\/j.colsurfb.2026.116165_bib9","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1038\/s41586-024-07580-0","article-title":"Mechanical release of homogenous proteins from supramolecular gels","volume":"631","author":"Bianco","year":"2024","journal-title":"Nature"},{"key":"10.1016\/j.colsurfb.2026.116165_bib10","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abc4824","article-title":"Injectable, photoresponsive hydrogels for delivering neuroprotective proteins enabled by metal-directed protein assembly","volume":"6","author":"Jiang","year":"2020","journal-title":"Sci. Adv."},{"key":"10.1016\/j.colsurfb.2026.116165_bib11","article-title":"High-concentration antibody formulation via solvent-based dehydration","volume":"38","author":"Zheng","year":"2026","journal-title":"Adv. Mater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2025.159275","article-title":"Dual antibody-guided drug delivery systems using MOF-PQDs nanocomposites for precise tumor diagnosis and combination therapy","volume":"505","author":"Han","year":"2025","journal-title":"Chem. Eng. J."},{"key":"10.1016\/j.colsurfb.2026.116165_bib13","doi-asserted-by":"crossref","DOI":"10.1002\/adfm.202001196","article-title":"Injectable polypeptide-protein hydrogels for promoting infected wound healing","volume":"30","author":"Cheng","year":"2020","journal-title":"Adv. Funct. Mater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib14","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1038\/s41467-025-56137-w","article-title":"Chiral polypeptide hydrogels regulating local immune microenvironment and anti-tumor immune response","volume":"16","author":"Ding","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.colsurfb.2026.116165_bib15","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1021\/acs.chemmater.3c02242","article-title":"Engineering hydrogels for affinity-based release of therapeutic proteins","volume":"36","author":"Teal","year":"2024","journal-title":"Chem. Mater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib16","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1039\/C2CS35358J","article-title":"Recombinant proteins as cross-linkers for hydrogelations","volume":"42","author":"Wang","year":"2013","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.colsurfb.2026.116165_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.jconrel.2025.113897","article-title":"Injectable hydrogel-based drug formulation for enhancing tertiary lymphoid structure formation and cancer immunotherapy efficacy","volume":"384","author":"Kuwentrai","year":"2025","journal-title":"J. Control. Release"},{"key":"10.1016\/j.colsurfb.2026.116165_bib18","article-title":"Self-transforming hydrogel mimicking tertiary lymph nodes to activate cGAS-STING pathway for enhanced antitumor immunotherapy","volume":"12","author":"Wang","year":"2026","journal-title":"Sci. Adv."},{"key":"10.1016\/j.colsurfb.2026.116165_bib19","doi-asserted-by":"crossref","first-page":"8585","DOI":"10.1021\/jacs.4c00569","article-title":"A programmable peptidic hydrogel adjuvant for personalized immunotherapy in resected stage tumors","volume":"146","author":"Wu","year":"2024","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.colsurfb.2026.116165_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.cis.2026.103906","article-title":"Crosslinking-driven design of protein hydrogels: mechanisms, engineering strategies, and innovative applications in food science","volume":"354","author":"Wang","year":"2026","journal-title":"Adv. Colloid Interface Sci."},{"key":"10.1016\/j.colsurfb.2026.116165_bib21","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1021\/acsmacrolett.2c00741","article-title":"Orchestrating chemical and physical cross-linking in protein hydrogels to regulate embryonic stem cell growth","volume":"12","author":"Yang","year":"2023","journal-title":"ACS Macro Lett."},{"key":"10.1016\/j.colsurfb.2026.116165_bib22","doi-asserted-by":"crossref","first-page":"2065","DOI":"10.1021\/acs.biomac.3c01451","article-title":"One-pot fabrication of supramolecular synthetic protein hydrogel with tissue-like integrated dynamic features","volume":"25","author":"Gao","year":"2024","journal-title":"Biomacromolecules"},{"key":"10.1016\/j.colsurfb.2026.116165_bib23","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1021\/polymscitech.5c00024","article-title":"Ionic crosslinking improves the stiffness and toughness of protein hydrogels","volume":"1","author":"Fu","year":"2025","journal-title":"Polym. Sci. Technol."},{"key":"10.1016\/j.colsurfb.2026.116165_bib24","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1021\/acs.biomac.4c01519","article-title":"Nanomachine networks: functional all-enzyme hydrogels from photochemical cross-linking of glucose oxidase","volume":"26","author":"Laurent","year":"2025","journal-title":"Biomacromolecules"},{"key":"10.1016\/j.colsurfb.2026.116165_bib25","doi-asserted-by":"crossref","first-page":"6067","DOI":"10.1039\/C8TB01949E","article-title":"Enzymatically cross-linked hydrogels based on a linear poly(ethylene glycol) analogue for controlled protein release and 3D cell culture","volume":"6","author":"Wang","year":"2018","journal-title":"J. Mater. Chem. B"},{"key":"10.1016\/j.colsurfb.2026.116165_bib26","doi-asserted-by":"crossref","DOI":"10.1002\/smll.202403376","article-title":"Ultra-strong protein-based hydrogels via promoting intermolecular entanglement of the amorphous region","volume":"20","author":"Fu","year":"2024","journal-title":"Small"},{"key":"10.1016\/j.colsurfb.2026.116165_bib27","article-title":"Stimuli-responsive protein hydrogels: from dynamic tuning of hydrogel mechanics to shape morphing","volume":"4","author":"Li","year":"2025","journal-title":"Supramol. Mater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib28","doi-asserted-by":"crossref","DOI":"10.1002\/anie.6692301","article-title":"Multicolor and attenuated light intensity responses in protein hydrogels arising from photoregulated crosslink dynamics","author":"Frank","year":"2026","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.colsurfb.2026.116165_bib29","doi-asserted-by":"crossref","first-page":"74","DOI":"10.3390\/pharmaceutics18010074","article-title":"Recent developments in protein-based hydrogels for advanced drug delivery applications","volume":"18","author":"Scopelliti","year":"2026","journal-title":"Pharmaceutics"},{"key":"10.1016\/j.colsurfb.2026.116165_bib30","first-page":"368","article-title":"Ultrafast crosslinking, strongly adhesive de novo protein hydrogels promote cartilage regeneration","volume":"56","author":"Nie","year":"2026","journal-title":"Bioact. Mater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib31","article-title":"Mechanically induced adaptive self-growing protein hydrogel","volume":"38","author":"Ma","year":"2026","journal-title":"Adv. Mater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib32","doi-asserted-by":"crossref","DOI":"10.1002\/adma.202509812","article-title":"Fully reshapeable and recyclable protein hydrogels","volume":"37","author":"Bian","year":"2025","journal-title":"Adv. Mater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib33","doi-asserted-by":"crossref","DOI":"10.3389\/fchem.2025.1618870","article-title":"Protein-based double-network hydrogels mimicking oral mucosa","volume":"13","author":"Zhang","year":"2025","journal-title":"Front. Chem."},{"key":"10.1016\/j.colsurfb.2026.116165_bib34","series-title":"Proc. Natl. Acad. Sci. U. S. A.","article-title":"Soft, strong, tough, and durable protein-based fiber hydrogels","author":"Wang","year":"2023"},{"key":"10.1016\/j.colsurfb.2026.116165_bib35","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.matt.2023.11.015","article-title":"Engineering stiff and tough protein-based hydrogels: Bridging the mechanical gap between muscle and cartilage","volume":"7","author":"Chen","year":"2024","journal-title":"Matter"},{"key":"10.1016\/j.colsurfb.2026.116165_bib36","doi-asserted-by":"crossref","first-page":"3716","DOI":"10.1002\/cjoc.70210","article-title":"Design of stiff, tough, and fast-recovery protein hydrogels","volume":"43","author":"Wang","year":"2025","journal-title":"Chin. J. Chem."},{"key":"10.1016\/j.colsurfb.2026.116165_bib37","doi-asserted-by":"crossref","first-page":"30788","DOI":"10.1021\/acsami.6b11287","article-title":"Protein-cross-linked hydrogels with tailored swelling and bioactivity performance: a comparative study","volume":"8","author":"Li","year":"2016","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"10.1016\/j.colsurfb.2026.116165_bib38","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.1021\/acsapm.9b00066","article-title":"Mechanically strong and highly tough prolamin protein hydrogels designed from double-cross-linked assembled networks","volume":"1","author":"Wang","year":"2019","journal-title":"ACS Appl. Polym. Mater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib39","series-title":"Proc. Natl. Acad. Sci. U. S. A.","article-title":"De novo design of modular protein hydrogels with programmable intra- and extracellular viscoelasticity","author":"Mout","year":"2024"},{"key":"10.1016\/j.colsurfb.2026.116165_bib40","doi-asserted-by":"crossref","first-page":"8358","DOI":"10.1021\/acs.biomac.5c00691","article-title":"Building thermosensitive single-component protein hydrogels from de novo designed homodimers","volume":"26","author":"Ning","year":"2025","journal-title":"Biomacromolecules"},{"key":"10.1016\/j.colsurfb.2026.116165_bib41","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/j.cell.2023.12.028","article-title":"De novo protein design\u2014from new structures to programmable functions","volume":"187","author":"Kortemme","year":"2024","journal-title":"Cell"},{"key":"10.1016\/j.colsurfb.2026.116165_bib42","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1038\/s41586-026-10328-7","article-title":"The past, present and future of de novo protein design","volume":"652","author":"Yang","year":"2026","journal-title":"Nature"},{"key":"10.1016\/j.colsurfb.2026.116165_bib43","doi-asserted-by":"crossref","first-page":"2974","DOI":"10.1038\/ncomms3974","article-title":"Forced protein unfolding leads to highly elastic and tough protein hydrogels","volume":"4","author":"Fang","year":"2013","journal-title":"Nat. Commun."},{"key":"10.1016\/j.colsurfb.2026.116165_bib44","doi-asserted-by":"crossref","first-page":"4466","DOI":"10.1021\/ja710295t","article-title":"De Novo design of strand-swapped \u03b2-hairpin hydrogels","volume":"130","author":"Nagarkar","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.colsurfb.2026.116165_bib45","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adx3472","article-title":"PhoCoil: A photodegradable and injectable single-component recombinant protein hydrogel for minimally invasive delivery and degradation","volume":"11","author":"Gregorio","year":"2025","journal-title":"Sci. Adv."},{"key":"10.1016\/j.colsurfb.2026.116165_bib46","doi-asserted-by":"crossref","DOI":"10.1016\/j.celbio.2025.100239","article-title":"Stimuli-triggered formation of de novo-designed protein biomaterials","volume":"2","author":"Gregorio","year":"2026","journal-title":"Cell. Biomater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib47","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/s41586-025-09269-4","article-title":"Data-driven de novo design of super-adhesive hydrogels","volume":"644","author":"Liao","year":"2025","journal-title":"Nature"},{"key":"10.1016\/j.colsurfb.2026.116165_bib48","doi-asserted-by":"crossref","DOI":"10.1063\/5.0273394","article-title":"Deep learning-driven protein structure prediction and design: key model developments by Nobel laureates and multi-domain applications","volume":"7","author":"Yang","year":"2026","journal-title":"Biophys. Rev."},{"key":"10.1016\/j.colsurfb.2026.116165_bib49","doi-asserted-by":"crossref","first-page":"17293","DOI":"10.1021\/jacs.5c03523","article-title":"Leveraging protein\u2013ligand and DNA interactions to control hydrogel mechanics","volume":"147","author":"Ramani","year":"2025","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.colsurfb.2026.116165_bib50","doi-asserted-by":"crossref","DOI":"10.1063\/5.0236947","article-title":"On the reversibility of amyloid fibril formation","volume":"6","author":"P\u00e1lmad\u00f3ttir","year":"2025","journal-title":"Biophys. Rev."},{"key":"10.1016\/j.colsurfb.2026.116165_bib51","doi-asserted-by":"crossref","DOI":"10.1063\/5.0314495","article-title":"Smart biomaterials and intelligent scaffolds for wound healing in tissue engineering","volume":"7","author":"Barua","year":"2026","journal-title":"Biophys. Rev."},{"key":"10.1016\/j.colsurfb.2026.116165_bib52","doi-asserted-by":"crossref","DOI":"10.26599\/NR.2025.94907012","article-title":"Protein-based functional hydrogel improves cutaneous nerve repair and diabetic wound healing","volume":"18","author":"Lu","year":"2025","journal-title":"Nano Res."},{"key":"10.1016\/j.colsurfb.2026.116165_bib53","article-title":"Dynamic slide-ring hydrogels with dityrosine-driven pH-responsive fluorescence: enabling 3d printing, enhanced drug delivery and regenerative therapy","volume":"15","author":"Li","year":"2026","journal-title":"Adv. Healthc. Mater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib54","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1038\/s41467-023-44607-y","article-title":"Shape morphing of hydrogels by harnessing enzyme enabled mechanoresponse","volume":"15","author":"Zhang","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.colsurfb.2026.116165_bib55","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1038\/s41467-021-27744-0","article-title":"Engineering shape memory and morphing protein hydrogels based on protein unfolding and folding","volume":"13","author":"Bian","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.colsurfb.2026.116165_bib56","article-title":"A general protein unfolding-chemical coupling strategy for pure protein hydrogels with mechanically strong and multifunctional properties","volume":"9","author":"Tang","year":"2022","journal-title":"Adv. Sci."},{"key":"10.1016\/j.colsurfb.2026.116165_bib57","doi-asserted-by":"crossref","first-page":"3859","DOI":"10.1038\/s41467-020-17698-0","article-title":"Protein-responsive protein release of supramolecular\/polymer hydrogel composite integrating enzyme activation systems","volume":"11","author":"Shigemitsu","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.colsurfb.2026.116165_bib58","doi-asserted-by":"crossref","first-page":"5290","DOI":"10.1021\/ja401075s","article-title":"Intein-triggered artificial protein hydrogels that support the immobilization of bioactive proteins","volume":"135","author":"Ramirez","year":"2013","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.colsurfb.2026.116165_bib59","doi-asserted-by":"crossref","first-page":"10667","DOI":"10.1021\/acsnano.2c02369","article-title":"Tuning protein hydrogel mechanics through modulation of nanoscale unfolding and entanglement in postgelation relaxation","volume":"16","author":"Hughes","year":"2022","journal-title":"ACS Nano"},{"key":"10.1016\/j.colsurfb.2026.116165_bib60","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.1039\/D4SM01413H","article-title":"Capturing the impact of protein unfolding on the dynamic assembly of protein networks","volume":"21","author":"Hughes","year":"2025","journal-title":"Soft Matter"},{"key":"10.1016\/j.colsurfb.2026.116165_bib61","doi-asserted-by":"crossref","first-page":"15760","DOI":"10.1021\/ja805634x","article-title":"Smart hydrogels containing adenylate kinase: translating substrate recognition into macroscopic motion","volume":"130","author":"Yuan","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.colsurfb.2026.116165_bib62","doi-asserted-by":"crossref","first-page":"5342","DOI":"10.1021\/acs.biomac.3c00810","article-title":"Development of thiol\u2013ene reaction-based HA hydrogel with sustained release of egf for enhanced skin wound healing","volume":"24","author":"Lee","year":"2023","journal-title":"Biomacromolecules"},{"key":"10.1016\/j.colsurfb.2026.116165_bib63","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1021\/acscentsci.8b00325","article-title":"A reversible and repeatable thiol\u2013ene bioconjugation for dynamic patterning of signaling proteins in hydrogels","volume":"4","author":"Grim","year":"2018","journal-title":"ACS Cent. Sci."},{"key":"10.1016\/j.colsurfb.2026.116165_bib64","doi-asserted-by":"crossref","first-page":"10759","DOI":"10.1039\/D3NJ01230A","article-title":"In situ formation of biocompatible and ductile protein-based hydrogels via Michael addition reaction and visible light crosslinking","volume":"47","author":"Tutar","year":"2023","journal-title":"New. J. Chem."},{"key":"10.1016\/j.colsurfb.2026.116165_bib65","doi-asserted-by":"crossref","first-page":"3050","DOI":"10.1038\/s41467-025-58338-9","article-title":"Advanced enzyme-assembled hydrogels for the remediation of contaminated water","volume":"16","author":"Zhang","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.colsurfb.2026.116165_bib66","article-title":"Design and functionality of trypsin-triggered, expandable bovine serum albumin-polyethylene glycol diacrylate hydrogel actuators","volume":"4","author":"Liu","year":"2024","journal-title":"Small Sci."},{"key":"10.1016\/j.colsurfb.2026.116165_bib67","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2025.123469","article-title":"Thiol-ene photoclick hydrogels reinforced with poly(protocatechualdehyde)-coated gallium doped bioactive glass nanoparticles for scarless healing of infected wound","volume":"324","author":"Jian","year":"2026","journal-title":"Biomaterials"},{"key":"10.1016\/j.colsurfb.2026.116165_bib68","doi-asserted-by":"crossref","first-page":"18842","DOI":"10.1021\/acs.jafc.3c05898","article-title":"Preparation of polysaccharide\u2013protein hydrogels with an ultrafast self-healing property as a superior oral delivery system of probiotics","volume":"71","author":"Qiu","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.colsurfb.2026.116165_bib69","article-title":"Autonomous hydrogel actuators programmed by endogenous biochemical logic for dual-stage morphing and drug release","volume":"38","author":"Liu","year":"2026","journal-title":"Adv. Mater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib70","doi-asserted-by":"crossref","DOI":"10.1002\/agt2.639","article-title":"Advancing self-healing soy protein hydrogel with dynamic Schiff base and metal-ligand bonds for diabetic chronic wound recovery","volume":"5","author":"Lan","year":"2024","journal-title":"Aggregate"},{"key":"10.1016\/j.colsurfb.2026.116165_bib71","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1007\/s13233-025-00368-9","article-title":"Enzyme-crosslinked hyaluronic acid-based multifunctional hydrogel promotes diabetic wound healing","volume":"33","author":"Cheng","year":"2025","journal-title":"Macromol. Res."},{"key":"10.1016\/j.colsurfb.2026.116165_bib72","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1021\/acs.bioconjchem.5c00088","article-title":"Light-controllable PEG hydrogel cross-linked by reversibly photodissociable dimeric green fluorescent protein pdDronpa for drug delivery","volume":"36","author":"Nguyen","year":"2025","journal-title":"Bioconjug. Chem."},{"key":"10.1016\/j.colsurfb.2026.116165_bib73","article-title":"Mechanically stable and tunable photoactivated peptide-based hydrogels for soft tissue adhesion","volume":"36","author":"Ross","year":"2026","journal-title":"Adv. Funct. Mater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib74","article-title":"Engineered protein hydrogels as biomimetic cellular scaffolds","volume":"36","author":"Liu","year":"2024","journal-title":"Adv. Mater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib75","doi-asserted-by":"crossref","first-page":"5991","DOI":"10.1021\/acs.chemrev.5c00159","article-title":"A review of hydrogel fiber: design, synthesis, applications, and futures","volume":"125","author":"Chen","year":"2025","journal-title":"Chem. Rev."},{"key":"10.1016\/j.colsurfb.2026.116165_bib76","doi-asserted-by":"crossref","DOI":"10.1016\/j.carbpol.2025.123407","article-title":"Protein-polysaccharide based nanogel\/hydrogel composite with controlled continuous delivery of drug for enhanced wound healing","volume":"356","author":"Mao","year":"2025","journal-title":"Carbohydr. Polym."},{"key":"10.1016\/j.colsurfb.2026.116165_bib77","doi-asserted-by":"crossref","first-page":"2812","DOI":"10.1021\/acs.biomac.6b00566","article-title":"Engineering protein hydrogels using SpyCatcher-SpyTag chemistry","volume":"17","author":"Gao","year":"2016","journal-title":"Biomacromolecules"},{"key":"10.1016\/j.colsurfb.2026.116165_bib78","article-title":"Bioinspired genetic and chemical engineering of protein hydrogels for programable multi-responsive actuation","volume":"13","author":"Ji","year":"2024","journal-title":"Adv. Healthc. Mater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib79","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1038\/s41467-025-56726-9","article-title":"Molecular self-assembly strategy tuning a dry crosslinking protein patch for biocompatible and biodegradable haemostatic sealing","volume":"16","author":"Yu","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.colsurfb.2026.116165_bib80","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1021\/acscentsci.6b00205","article-title":"Engineering the dynamic properties of protein networks through sequence variation","volume":"2","author":"Dooling","year":"2016","journal-title":"ACS Cent. Sci."},{"key":"10.1016\/j.colsurfb.2026.116165_bib81","doi-asserted-by":"crossref","first-page":"22067","DOI":"10.1073\/pnas.0904851106","article-title":"Two-component protein-engineered physical hydrogels for cell encapsulation","volume":"106","author":"Wong Po Foo","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.colsurfb.2026.116165_bib82","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2115523119","article-title":"Conformation-driven strategy for resilient and functional protein materials","volume":"119","author":"Mu","year":"2022","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.colsurfb.2026.116165_bib83","doi-asserted-by":"crossref","DOI":"10.1002\/adfm.202405255","article-title":"Multifunctional hydrogel based on silk fibroin promotes tissue repair and regeneration","volume":"34","author":"Lin","year":"2024","journal-title":"Adv. Funct. Mater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib84","doi-asserted-by":"crossref","DOI":"10.1016\/j.carbpol.2024.123056","article-title":"Antifreeze proteins and surface-modified cellulose nanocrystals for designing anti-freezing conductive hydrogel sensors","volume":"350","author":"Bai","year":"2025","journal-title":"Carbohydr. Polym."},{"key":"10.1016\/j.colsurfb.2026.116165_bib85","doi-asserted-by":"crossref","first-page":"7404","DOI":"10.1021\/acsbiomaterials.4c01584","article-title":"The photocleavable protein PhoCl-based dynamic hydrogels","volume":"10","author":"Lei","year":"2024","journal-title":"ACS Biomater. Sci. Eng."},{"key":"10.1016\/j.colsurfb.2026.116165_bib86","doi-asserted-by":"crossref","first-page":"8835","DOI":"10.1021\/acs.chemrev.5c00182","article-title":"Therapeutic hydrogels: properties and biomedical applications","volume":"125","author":"Dong","year":"2025","journal-title":"Chem. Rev."},{"key":"10.1016\/j.colsurfb.2026.116165_bib87","doi-asserted-by":"crossref","DOI":"10.1016\/j.colsurfb.2026.115667","article-title":"Construction of anti-HER2 conjugated injectable hydrogel via protein crosslinking and gadolinium-tetraaza coordination for multimodal tumor theranostics","volume":"264","author":"Gao","year":"2026","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"10.1016\/j.colsurfb.2026.116165_bib88","doi-asserted-by":"crossref","DOI":"10.1063\/5.0236610","article-title":"The mechanobiology of biomolecular condensates","volume":"6","author":"Sanfeliu-Cerd\u00e1n","year":"2025","journal-title":"Biophys. Rev."},{"key":"10.1016\/j.colsurfb.2026.116165_bib89","doi-asserted-by":"crossref","first-page":"3050","DOI":"10.1021\/acs.jafc.9b07540","article-title":"Construction of a Lignosulfonate\u2013Lysine Hydrogel for the Adsorption of Heavy Metal Ions","volume":"68","author":"Jiang","year":"2020","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.colsurfb.2026.116165_bib90","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1021\/acsabm.2c00951","article-title":"In situ-forming protein-polymer hydrogel for glucose-responsive insulin release","volume":"6","author":"Ali","year":"2023","journal-title":"ACS Appl. Bio Mater."},{"key":"10.1016\/j.colsurfb.2026.116165_bib91","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodchem.2025.146329","article-title":"Development and characterization of BSA-pullulan gel matrices incorporating orange peel flavonoids: Structural interactions and antimicrobial and antioxidant functionalities","volume":"495","author":"Salah","year":"2025","journal-title":"Food Chem."},{"key":"10.1016\/j.colsurfb.2026.116165_bib92","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.adr3209","article-title":"Synthetic, multi-dynamic hydrogels by uniting stress-stiffening and supramolecular polymers","volume":"10","author":"Rijns","year":"2024","journal-title":"Sci. Adv."},{"key":"10.1016\/j.colsurfb.2026.116165_bib93","doi-asserted-by":"crossref","DOI":"10.1111\/1541-4337.70187","article-title":"Emerging Trends and Future Prospects of Peptide-Based Hydrogels: Revolutionizing Food Technology Applications","volume":"24","author":"Li","year":"2025","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"10.1016\/j.colsurfb.2026.116165_bib94","doi-asserted-by":"crossref","first-page":"4820","DOI":"10.1021\/acs.jafc.8b00233","article-title":"Development of functional or medical foods for oral administration of insulin for diabetes treatment: gastroprotective edible microgels","volume":"66","author":"Sun","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.colsurfb.2026.116165_bib95","article-title":"Intelligent design and application of molecular recognition hydrogels in tissue engineering","volume":"38","author":"Xue","year":"2026","journal-title":"Mater. Today Bio"},{"key":"10.1016\/j.colsurfb.2026.116165_bib96","doi-asserted-by":"crossref","first-page":"7918","DOI":"10.1021\/acs.chemrev.5c00069","article-title":"Noncovalent aggregation for diverse properties in hydrogels: a comprehensive review","volume":"125","author":"Duan","year":"2025","journal-title":"Chem. Rev."},{"key":"10.1016\/j.colsurfb.2026.116165_bib97","doi-asserted-by":"crossref","first-page":"5439","DOI":"10.1038\/s41467-019-13312-0","article-title":"Chemical unfolding of protein domains induces shape change in programmed protein hydrogels","volume":"10","author":"Khoury","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.colsurfb.2026.116165_bib98","doi-asserted-by":"crossref","first-page":"2291","DOI":"10.1039\/D1MA00960E","article-title":"Challenges and opportunities of silk protein hydrogels in biomedical applications","volume":"3","author":"Liu","year":"2022","journal-title":"Mater. Adv."},{"key":"10.1016\/j.colsurfb.2026.116165_bib99","doi-asserted-by":"crossref","DOI":"10.1016\/j.jcis.2025.138149","article-title":"Structural and mechanical scaling law behaviour in folded protein hydrogel networks","volume":"699","author":"Boroumand","year":"2025","journal-title":"J. Colloid Interface Sci."},{"key":"10.1016\/j.colsurfb.2026.116165_bib100","doi-asserted-by":"crossref","DOI":"10.1002\/slct.202405618","article-title":"A comprehensive review on protein-based hydrogels: from structure modification to applications","volume":"10","author":"Gou","year":"2025","journal-title":"ChemistrySelect"}],"container-title":["Colloids and Surfaces B: Biointerfaces"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0927776526007538?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0927776526007538?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T00:04:17Z","timestamp":1793923457000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0927776526007538"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,1]]},"references-count":100,"alternative-id":["S0927776526007538"],"URL":"https:\/\/doi.org\/10.1016\/j.colsurfb.2026.116165","relation":{},"ISSN":["0927-7765"],"issn-type":[{"value":"0927-7765","type":"print"}],"subject":[],"published":{"date-parts":[[2027,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Programmable crosslinking strategies for the rational design of engineered protein hydrogels","name":"articletitle","label":"Article Title"},{"value":"Colloids and Surfaces B: Biointerfaces","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.colsurfb.2026.116165","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"116165"}}