{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T16:58:06Z","timestamp":1788368286702,"version":"build-2803163510"},"reference-count":50,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:00:00Z","timestamp":1747785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Foundation","doi-asserted-by":"publisher","award":["NNF23OC0086233"],"award-info":[{"award-number":["NNF23OC0086233"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018703","name":"European Innovation Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018703","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005192","name":"Technical University of Denmark","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005192","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bmn.com","clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Current Opinion in Biotechnology"],"published-print":{"date-parts":[[2025,8]]},"DOI":"10.1016\/j.copbio.2025.103319","type":"journal-article","created":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T22:58:57Z","timestamp":1748559537000},"page":"103319","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Operation at thermophilic temperatures: an underestimated asset for gas fermentation processes"],"prefix":"10.1016","volume":"94","author":[{"given":"Hariklia N","family":"Gavala","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis V","family":"Skiadas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.copbio.2025.103319_bib1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s10311-020-01094-7","article-title":"Thermochemical conversion of plastic waste to fuels: a review","volume":"19","author":"Nanda","year":"2021","journal-title":"Environ Chem Lett"},{"key":"10.1016\/j.copbio.2025.103319_bib2","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1016\/j.rser.2017.05.262","article-title":"Thermochemical processing of sewage sludge to energy and fuel: fundamentals, challenges and considerations","volume":"80","author":"Syed-Hassan","year":"2017","journal-title":"Renew Sustain Energy Rev"},{"key":"10.1016\/j.copbio.2025.103319_bib3","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.wasman.2017.08.046","article-title":"A review on technological options of waste to energy for effective management of municipal solid waste","volume":"69","author":"Kumar","year":"2017","journal-title":"Waste Manag"},{"key":"10.1016\/j.copbio.2025.103319_bib4","unstructured":"Statista. Natural gas consumption worldwide \u3008Https:\/\/www.statista.com\/statistics\/282717\/global-natural-gas-consumption\/\u3009."},{"key":"10.1016\/j.copbio.2025.103319_bib5","unstructured":"Statista. Production capacity of acetic acid worldwide in 2018 and 2023 \u3008https:\/\/www.statista.com\/statistics\/1063215\/acetic-acid-production-capacity-globally\/\u3009."},{"key":"10.1016\/j.copbio.2025.103319_bib6","unstructured":"Statista. Market volume of n-butanol worldwide \u3008https:\/\/www.statista.com\/statistics\/1245211\/n-butanol-market-volume-worldwide\/#:\u223c:text=In%202021%2C%20the%20market%20volume%20of%20n-Butanol%20worldwide,million%20metric%20tons%20worldwide%20in%20the%20year%202029\u3009."},{"key":"10.1016\/j.copbio.2025.103319_bib7","unstructured":"OECD-FAO. Biofuels. In\u00a0Agricultural Outlook 2020\u20132029 \u3008https:\/\/www.oecd-ilibrary.org\/sites\/3aeb7be3-en\/index.html?itemId=\/content\/component\/3aeb7be3-en\u3009."},{"key":"10.1016\/j.copbio.2025.103319_bib8","unstructured":"Haufather\u00a0Z, Friedlingstein P: Carbon brief\u00a02023. Analysis: Global CO2 Emissions Will Reach New High in 2024 despite Slower Growth. Available from: \u3008https:\/\/www.carbonbrief.org\/analysis-global-co2-emissions-will-reach-new-high-in-2024-despite-slower-growth\/\u3009."},{"key":"10.1016\/j.copbio.2025.103319_sbref4","doi-asserted-by":"crossref","first-page":"5303","DOI":"10.1007\/s12649-020-01332-7","article-title":"Gas biological conversions: the potential of syngas and carbon dioxide as production platforms","volume":"12","author":"Gavala","year":"2021","journal-title":"Waste Biomass Valoriz"},{"key":"10.1016\/j.copbio.2025.103319_bib10","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1146\/annurev-chembioeng-120120-021122","article-title":"Stepping on the gas to a circular economy: accelerating development of carbon-negative chemical production from gas fermentation","volume":"12","author":"Fackler","year":"2021","journal-title":"Annu Rev Chem Biomol Eng"},{"key":"10.1016\/j.copbio.2025.103319_sbref6","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1007\/s11157-022-09627-y","article-title":"Prospective CO2 and CO bioconversion into ectoines using novel microbial platforms","volume":"21","author":"Cantera","year":"2022","journal-title":"Rev Environ Sci Biotechnol"},{"key":"10.1016\/j.copbio.2025.103319_sbref7","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.copbio.2019.07.003","article-title":"Mixed carbon substrates: a necessary nuisance or a missed opportunity?","volume":"62","author":"Liu","year":"2020","journal-title":"Curr Opin Biotechnol"},{"key":"10.1016\/j.copbio.2025.103319_sbref8","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1038\/s42255-019-0077-0","article-title":"Synergistic substrate cofeeding stimulates reductive metabolism","volume":"1","author":"Park","year":"2019","journal-title":"Nat Metab"},{"key":"10.1016\/j.copbio.2025.103319_bib14","first-page":"146","article-title":"A preliminary experimental confirmation of the two-film theory of gas absorption","volume":"29","author":"Whitman","year":"1923","journal-title":"Chem Metall Eng"},{"key":"10.1016\/j.copbio.2025.103319_sbref10","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1016\/j.cej.2018.05.003","article-title":"Reactor systems for syngas fermentation processes: a review","volume":"348","author":"Asimakopoulos","year":"2018","journal-title":"Chem Eng J"},{"key":"10.1016\/j.copbio.2025.103319_sbref11","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2019.122129","article-title":"Effects of moderately elevated pressure on gas fermentation processes","volume":"293","author":"Van Hecke","year":"2019","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.copbio.2025.103319_bib17","unstructured":"CRC. CRC Handbook of Chemistry and Physics (chemnetbase.com). 103rd Edit. Rumble, John R, editor."},{"key":"10.1016\/j.copbio.2025.103319_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.rser.2021.111209","article-title":"H2 gas-liquid mass transfer: a key element in biological Power-to-Gas methanation","volume":"147","author":"Jensen","year":"2021","journal-title":"Renew Sustain Energy Rev"},{"key":"10.1016\/j.copbio.2025.103319_sbref13","doi-asserted-by":"crossref","DOI":"10.1016\/j.jclepro.2024.143433","article-title":"Dynamic tank-in-series modelling and simulation of gas-liquid interaction in trickle bed reactor designed for gas fermentation","volume":"472","author":"Dutta","year":"2024","journal-title":"J Clean Prod"},{"key":"10.1016\/j.copbio.2025.103319_sbref14","doi-asserted-by":"crossref","DOI":"10.3390\/fermentation10120623","article-title":"Scaling up Trickle Bed Reactors for gas fermentation technology: the effect of temperature and reactor characteristics on mass transfer","volume":"10","author":"Dutta","year":"2024","journal-title":"Fermentation"},{"key":"10.1016\/j.copbio.2025.103319_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.apenergy.2020.114502","article-title":"Modeling of syngas biomethanation and catabolic route control in mesophilic and thermophilic mixed microbial consortia","volume":"262","author":"Grimalt-Alemany","year":"2020","journal-title":"Appl Energy"},{"key":"10.1016\/j.copbio.2025.103319_bib22","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1016\/j.renene.2015.07.066","article-title":"Renewable Power-to-Gas: a technological and economic review","volume":"85","author":"Goetz","year":"2016","journal-title":"Renew Energy"},{"key":"10.1016\/j.copbio.2025.103319_bib23","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1002\/bit.24360","article-title":"Simultaneous hydrogen utilization and in situ biogas upgrading in an anaerobic reactor","volume":"109","author":"Luo","year":"2012","journal-title":"Biotechnol Bioeng"},{"key":"10.1016\/j.copbio.2025.103319_sbref18","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.biotechadv.2018.01.011","article-title":"Biogas upgrading and utilization: current status and perspectives","volume":"36","author":"Angelidaki","year":"2018","journal-title":"Biotechnol Adv"},{"key":"10.1016\/j.copbio.2025.103319_bib25","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.energy.2017.06.133","article-title":"Enrichment of hydrogenotrophic methanogens by means of gas recycle and its application in biogas upgrading","volume":"135","author":"Yun","year":"2017","journal-title":"Energy"},{"key":"10.1016\/j.copbio.2025.103319_bib26","doi-asserted-by":"crossref","first-page":"15434","DOI":"10.1016\/j.ijhydene.2022.02.072","article-title":"Evaluation of ex-situ hydrogen biomethanation at mesophilic and thermophilic temperatures","volume":"47","author":"Kozak","year":"2022","journal-title":"Int J Hydrog Energy"},{"key":"10.1016\/j.copbio.2025.103319_bib27","doi-asserted-by":"crossref","DOI":"10.3390\/molecules25235665","article-title":"Temperature and inoculum origin influence the performance of ex-situ biological hydrogen methanation","volume":"25","author":"Figeac","year":"2020","journal-title":"Molecules"},{"key":"10.1016\/j.copbio.2025.103319_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.fuproc.2022.107478","article-title":"Identification of the biomethanation pathways during biological CO2 fixation with exogenous H2 addition","volume":"238","author":"Jiang","year":"2022","journal-title":"Fuel Process Technol"},{"key":"10.1016\/j.copbio.2025.103319_sbref23","first-page":"465","article-title":"Enrichment of mesophilic and thermophilic mixed microbial consortia for syngas biomethanation: the role of kinetic and thermodynamic competition","author":"Grimalt-Alemany","year":"2019","journal-title":"Waste Biomass Valoriz"},{"key":"10.1016\/j.copbio.2025.103319_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2021.125455","article-title":"Characteristics of in-situ hydrogen biomethanation at mesophilic and thermophilic temperatures","volume":"337","author":"Jiang","year":"2021","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.copbio.2025.103319_sbref25","article-title":"Load change capability of an anaerobic thermophilic trickle bed reactor for dynamic H2\/CO2 biomethanation","volume":"289","author":"Struebing","year":"2019","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.copbio.2025.103319_bib32","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1016\/j.biortech.2017.08.088","article-title":"High performance biological methanation in a thermophilic anaerobic trickle bed reactor","volume":"245","author":"Str\u00fcbing","year":"2017","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.copbio.2025.103319_bib33","series-title":"Biomethanation of synthesis gas in trickle bed reactors","article-title":"Conclusions and outlook","author":"Asimakopoulos","year":"2019"},{"key":"10.1016\/j.copbio.2025.103319_bib34","doi-asserted-by":"crossref","first-page":"2006","DOI":"10.1021\/es102728m","article-title":"Potential of wastewater-treating anaerobic granules for biomethanation of synthesis gas","volume":"45","author":"Guiot","year":"2011","journal-title":"Environ Sci Technol"},{"key":"10.1016\/j.copbio.2025.103319_sbref29","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.wasman.2023.03.011","article-title":"Syngas biomethanation: in a transfer limited process, is CO inhibition an issue?","volume":"162","author":"Figueras","year":"2023","journal-title":"Waste Manag"},{"key":"10.1016\/j.copbio.2025.103319_bib36","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.biortech.2014.07.071","article-title":"Rapid bio-methanation of syngas in a reverse membrane bioreactor: membrane encased microorganisms","volume":"178","author":"Youngsukkasem","year":"2015","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.copbio.2025.103319_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.cej.2020.124739","article-title":"Temperature effects on syngas biomethanation performed in a trickle bed reactor","author":"Asimakopoulos","year":"2020","journal-title":"Chem Eng J"},{"key":"10.1016\/j.copbio.2025.103319_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2020.128649","article-title":"Homoacetogenesis and solventogenesis from H2\/CO2 by granular sludge at 25, 37 and 55 \u00b0C","volume":"265","author":"He","year":"2021","journal-title":"Chemosphere"},{"key":"10.1016\/j.copbio.2025.103319_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.jclepro.2023.137549","article-title":"Controlling the microbial competition between hydrogenotrophic methanogens and homoacetogens using mass transfer and thermodynamic constraints","volume":"414","author":"Laguillaumie","year":"2023","journal-title":"J Clean Prod"},{"key":"10.1016\/j.copbio.2025.103319_sbref34","doi-asserted-by":"crossref","DOI":"10.1016\/j.chemosphere.2022.134425","article-title":"Enhanced solventogenesis in syngas bioconversion: role of process parameters and thermodynamics","volume":"299","author":"He","year":"2022","journal-title":"Chemosphere"},{"key":"10.1016\/j.copbio.2025.103319_bib41","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.indcrop.2016.11.015","article-title":"Exploiting the potential of gas fermentation","volume":"106","author":"Redl","year":"2017","journal-title":"Ind Crops Prod"},{"key":"10.1016\/j.copbio.2025.103319_bib42","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2024.1476253","article-title":"Progresses and challenges of engineering thermophilic acetogenic cell factories","volume":"15","author":"Bourgade","year":"2024","journal-title":"Front Microbiol"},{"key":"10.1016\/j.copbio.2025.103319_bib43","doi-asserted-by":"crossref","first-page":"2619","DOI":"10.1007\/s00253-017-8124-9","article-title":"Enhancement of acetate productivity in a thermophilic (55 \u00b0C) hollow-fiber membrane biofilm reactor with mixed culture syngas (H2\/CO2) fermentation","volume":"101","author":"Wang","year":"2017","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.copbio.2025.103319_bib44","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/j.jclepro.2018.08.162","article-title":"Conversion of syngas (CO and H2) to biochemicals by mixed culture fermentation in mesophilic and thermophilic hollow-fiber membrane biofilm reactors","volume":"202","author":"Shen","year":"2018","journal-title":"J Clean Prod"},{"key":"10.1016\/j.copbio.2025.103319_bib45","doi-asserted-by":"crossref","DOI":"10.3390\/fermentation9090826","article-title":"Acetate production by Moorella thermoacetica via syngas fermentation: effect of yeast extract and syngas composition","volume":"9","author":"Harahap","year":"2023","journal-title":"Fermentation"},{"key":"10.1016\/j.copbio.2025.103319_bib46","doi-asserted-by":"crossref","DOI":"10.1186\/s13068-024-02460-1","article-title":"Isopropanol production via the thermophilic bioconversion of sugars and syngas using metabolically engineered Moorella thermoacetica","volume":"17","author":"Kato","year":"2024","journal-title":"Biotechnol Biofuels Bioprod"},{"key":"10.1016\/j.copbio.2025.103319_bib47","doi-asserted-by":"crossref","DOI":"10.1186\/s13568-021-01220-w","article-title":"Metabolic engineering of Moorella thermoacetica for thermophilic bioconversion of gaseous substrates to a volatile chemical","volume":"11","author":"Kato","year":"2021","journal-title":"AMB Express"},{"key":"10.1016\/j.copbio.2025.103319_sbref42","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2024.131480","article-title":"Bioconversion of pure CO2 to caproic acid with zero valent iron: optimizing carbon flux distribution in co-cultures of Acetobacterium woodii and Megasphaera hexanoica","volume":"413","author":"Wang","year":"2024","journal-title":"Bioresour Technol"},{"key":"10.1016\/j.copbio.2025.103319_bib49","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2023.169509","article-title":"Effect of electron acceptors on product selectivity and carbon flux in carbon chain elongation with Megasphaera hexanoica","volume":"912","author":"Wang","year":"2024","journal-title":"Sci Total Environ"},{"key":"10.1016\/j.copbio.2025.103319_bib50","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2024.131138","article-title":"Comprehensive comparative study on n-caproate production by Clostridium kluyveri: batch vs. continuous operation modes","volume":"408","author":"Fernandez-Blanco","year":"2024","journal-title":"Bioresour Technol"}],"container-title":["Current Opinion in Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0958166925000631?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0958166925000631?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T11:15:57Z","timestamp":1762341357000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0958166925000631"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":50,"alternative-id":["S0958166925000631"],"URL":"https:\/\/doi.org\/10.1016\/j.copbio.2025.103319","relation":{},"ISSN":["0958-1669"],"issn-type":[{"value":"0958-1669","type":"print"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Operation at thermophilic temperatures: an underestimated asset for gas fermentation processes","name":"articletitle","label":"Article Title"},{"value":"Current Opinion in Biotechnology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.copbio.2025.103319","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"103319"}}