{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T11:37:10Z","timestamp":1761824230251,"version":"build-2065373602"},"reference-count":79,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Current Opinion in Virology"],"published-print":{"date-parts":[[2020,10]]},"DOI":"10.1016\/j.coviro.2020.05.002","type":"journal-article","created":{"date-parts":[[2020,6,18]],"date-time":"2020-06-18T12:11:45Z","timestamp":1592482305000},"page":"7-15","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Reverse genetic approaches for the development of Zika vaccines and therapeutics"],"prefix":"10.1016","volume":"44","author":[{"given":"Camila R","family":"Fontes-Garfias","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7252-5066","authenticated-orcid":false,"given":"Coleman K","family":"Baker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei-Yong","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.coviro.2020.05.002_bib0005","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.cell.2016.09.020","article-title":"Zika virus: immunity and vaccine development","volume":"167","author":"Pierson","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.coviro.2020.05.002_bib0010","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.antiviral.2016.03.010","article-title":"Zika virus: history, emergence, biology, and prospects for control","volume":"130","author":"Weaver","year":"2016","journal-title":"Antiviral Res"},{"volume":"vol 1","year":"2013","author":"Pierson","key":"10.1016\/j.coviro.2020.05.002_bib0015"},{"key":"10.1016\/j.coviro.2020.05.002_bib0020","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1038\/nrmicro.2016.125","article-title":"Zika virus - reigniting the TORCH","volume":"14","author":"Coyne","year":"2016","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.coviro.2020.05.002_bib0025","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1056\/NEJMra1602113","article-title":"Zika virus","volume":"374","author":"Petersen","year":"2016","journal-title":"N Engl J Med"},{"key":"10.1016\/j.coviro.2020.05.002_bib0030","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1001\/jama.2016.2899","article-title":"The emerging Zika virus epidemic in the Americas: research priorities","volume":"315","author":"Lazear","year":"2016","journal-title":"JAMA"},{"key":"10.1016\/j.coviro.2020.05.002_bib0035","doi-asserted-by":"crossref","first-page":"329","DOI":"10.15585\/mmwr.mm6612a4","article-title":"Zika virus transmission - region of the Americas, May 15, 2015-December 15, 2016","volume":"66","author":"Ikejezie","year":"2017","journal-title":"MMWR Morb Mortal Wkly Rep"},{"year":"2012","series-title":"Molecular Virology and Control of Flaviviruses","author":"Shi","key":"10.1016\/j.coviro.2020.05.002_bib0040"},{"key":"10.1016\/j.coviro.2020.05.002_bib0045","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.antiviral.2017.06.001","article-title":"Zika in the Americas, year 2: what have we learned? What gaps remain? A report from the global virus network","volume":"144","author":"Aliota","year":"2017","journal-title":"Antiviral Res"},{"key":"10.1016\/j.coviro.2020.05.002_bib0050","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1056\/NEJMp1600297","article-title":"Zika virus in the Americas\u2014yet another arbovirus threat","volume":"374","author":"Fauci","year":"2016","journal-title":"N Engl J Med"},{"key":"10.1016\/j.coviro.2020.05.002_bib0055","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.cell.2017.06.040","article-title":"Vaccine mediated protection against Zika virus-induced congenital disease","volume":"170","author":"Richner","year":"2017","journal-title":"Cell"},{"key":"10.1016\/j.coviro.2020.05.002_bib0060","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.chom.2018.05.021","article-title":"Zika virus vaccine: progress and challenges","volume":"24","author":"Shan","year":"2018","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.coviro.2020.05.002_bib0065","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.ebiom.2016.09.013","article-title":"Zika virus replicons for drug discovery","volume":"12","author":"Xie","year":"2016","journal-title":"EBioMedicine"},{"key":"10.1016\/j.coviro.2020.05.002_bib0070","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1016\/j.chom.2016.05.004","article-title":"An infectious cDNA clone of Zika virus to study viral virulence, mosquito transmission, and antiviral inhibitors","volume":"19","author":"Shan","year":"2016","journal-title":"Cell Host Microbe"},{"year":"2007","series-title":"Virology: Principles and Applications","author":"Carter","key":"10.1016\/j.coviro.2020.05.002_bib0075"},{"key":"10.1016\/j.coviro.2020.05.002_bib0080","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01114-16","article-title":"A full-length infectious cDNA clone of Zika virus from the 2015 epidemic in Brazil as a genetic platform for studies of virus-host interactions and vaccine development","volume":"7","author":"Tsetsarkin","year":"2016","journal-title":"mBio"},{"key":"10.1016\/j.coviro.2020.05.002_bib0085","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/978-1-4939-6964-7_4","article-title":"Reverse genetics of Zika virus","volume":"1602","author":"Shan","year":"2017","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.coviro.2020.05.002_bib0090","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00484-17","article-title":"Characterization of cis-acting RNA elements of Zika virus by using a self-splicing ribozyme-dependent infectious clone","volume":"91","author":"Liu","year":"2017","journal-title":"J Virol"},{"key":"10.1016\/j.coviro.2020.05.002_bib0095","article-title":"Development and characterization of recombinant virus generated from a new world Zika virus infectious clone","volume":"91","author":"Weger-Lucarelli","year":"2017","journal-title":"J Virol"},{"key":"10.1016\/j.coviro.2020.05.002_bib0100","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02014-16","article-title":"A reverse genetics platform that spans the Zika virus family tree","volume":"8","author":"Widman","year":"2017","journal-title":"mBio"},{"key":"10.1016\/j.coviro.2020.05.002_bib0105","doi-asserted-by":"crossref","DOI":"10.3390\/v10070368","article-title":"A reverse genetics system for Zika virus based on a simple molecular cloning strategy","volume":"10","author":"M\u00fcnster","year":"2018","journal-title":"Viruses"},{"key":"10.1016\/j.coviro.2020.05.002_bib0110","doi-asserted-by":"crossref","DOI":"10.1128\/mSphereDirect.00190-17","article-title":"Generation and characterization of new Zika virus isolate using sequence data from a microcephaly case","volume":"2","author":"Setoh","year":"2017","journal-title":"mSphere"},{"key":"10.1016\/j.coviro.2020.05.002_bib0115","doi-asserted-by":"crossref","first-page":"39384","DOI":"10.1038\/srep39384","article-title":"Simple reverse genetics systems for Asian and African Zika viruses","volume":"6","author":"Atieh","year":"2016","journal-title":"Sci Rep"},{"key":"10.1016\/j.coviro.2020.05.002_bib0120","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1038\/nature22365","article-title":"Evolutionary enhancement of Zika virus infectivity in Aedes aegypti mosquitoes","volume":"545","author":"Liu","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.coviro.2020.05.002_bib0125","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-017-02816-2","article-title":"An evolutionary NS1 mutation enhances Zika virus evasion of host interferon induction","volume":"9","author":"Xia","year":"2018","journal-title":"Nat Commun"},{"key":"10.1016\/j.coviro.2020.05.002_bib0130","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1126\/science.aam7120","article-title":"A single mutation in the prM protein of Zika virus contributes to fetal microcephaly","volume":"358","author":"Yuan","year":"2017","journal-title":"Science"},{"key":"10.1016\/j.coviro.2020.05.002_bib0135","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02375-19","article-title":"Zika virus NS2A-mediated virion assembly","volume":"10","author":"Zhang","year":"2019","journal-title":"mBio"},{"key":"10.1016\/j.coviro.2020.05.002_bib0140","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1038\/s41541-019-0143-3","article-title":"An attenuated Zika virus NS4B protein mutant is a potent inducer of antiviral immune responses","volume":"4","author":"Li","year":"2019","journal-title":"NPJ Vaccines"},{"key":"10.1016\/j.coviro.2020.05.002_bib0145","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.ebiom.2018.08.056","article-title":"A single-dose plasmid-launched live-attenuated Zika vaccine induces protective immunity","volume":"36","author":"Zou","year":"2018","journal-title":"EBioMedicine"},{"key":"10.1016\/j.coviro.2020.05.002_bib0150","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02134-16","article-title":"Understanding Zika virus stability and developing a chimeric vaccine through functional analysis","volume":"8","author":"Xie","year":"2017","journal-title":"mBio"},{"key":"10.1016\/j.coviro.2020.05.002_bib0155","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1186\/s12916-018-1067-x","article-title":"Zika vaccines and therapeutics: landscape analysis and challenges ahead","volume":"16","author":"Wilder-Smith","year":"2018","journal-title":"BMC Med"},{"key":"10.1016\/j.coviro.2020.05.002_bib0160","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.chom.2018.09.008","article-title":"A single-dose live-attenuated Zika virus vaccine with controlled infection rounds that protects against vertical transmission","volume":"24","author":"Xie","year":"2018","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.coviro.2020.05.002_bib0165","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/s41541-018-0061-9","article-title":"Current status of Zika vaccine development: Zika vaccines advance into clinical evaluation","volume":"3","author":"Barrett","year":"2018","journal-title":"NPJ Vaccines"},{"key":"10.1016\/j.coviro.2020.05.002_bib0170","doi-asserted-by":"crossref","DOI":"10.1016\/j.antiviral.2019.104596","article-title":"Genetic stability of live-attenuated Zika vaccine candidates","volume":"171","author":"Muruato","year":"2019","journal-title":"Antiviral Res"},{"key":"10.1016\/j.coviro.2020.05.002_bib0175","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1006\/viro.1996.0406","article-title":"Mutagenesis of the N-linked glycosylation sites of the yellow fever virus NS1 protein: effects on virus replication and mouse neurovirulence","volume":"222","author":"Muylaert","year":"1996","journal-title":"Virology"},{"key":"10.1016\/j.coviro.2020.05.002_bib0180","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1038\/nm.4322","article-title":"A live-attenuated Zika virus vaccine candidate induces sterilizing immunity in mouse models","volume":"23","author":"Shan","year":"2017","journal-title":"Nat Med"},{"key":"10.1016\/j.coviro.2020.05.002_bib0185","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-017-00737-8","article-title":"A single-dose live-attenuated vaccine prevents Zika virus pregnancy transmission and testis damage","volume":"8","author":"Shan","year":"2017","journal-title":"Nat Commun"},{"key":"10.1016\/j.coviro.2020.05.002_bib0190","doi-asserted-by":"crossref","first-page":"1180","DOI":"10.1016\/j.celrep.2017.10.016","article-title":"Functional analysis of glycosylation of Zika virus envelope protein","volume":"21","author":"Fontes-Garfias","year":"2017","journal-title":"Cell Rep"},{"key":"10.1016\/j.coviro.2020.05.002_bib0195","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1126\/science.aai9137","article-title":"Rapid development of a DNA vaccine for Zika virus","volume":"354","author":"Dowd","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.coviro.2020.05.002_bib0200","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1038\/nature18952","article-title":"Vaccine protection against Zika virus from Brazil","volume":"536","author":"Larocca","year":"2016","journal-title":"Nature"},{"key":"10.1016\/j.coviro.2020.05.002_bib0205","article-title":"Development of a chimeric Zika vaccine using a licensed live-attenuated flavivirus vaccine as backbone","volume":"9","author":"Li","year":"2018","journal-title":"Nat Commun"},{"key":"10.1016\/j.coviro.2020.05.002_bib0210","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/B978-0-12-800098-4.00007-6","article-title":"Dengue virus vaccine development","volume":"88","author":"Yauch","year":"2014","journal-title":"Adv Virus Res"},{"key":"10.1016\/j.coviro.2020.05.002_bib0215","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1177\/2051013613507862","article-title":"Latest developments and future directions in dengue vaccines","volume":"2","author":"Thisyakorn","year":"2014","journal-title":"Ther Adv Vaccines"},{"key":"10.1016\/j.coviro.2020.05.002_bib0220","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1086\/378356","article-title":"Chimeric live, attenuated vaccine against Japanese encephalitis (ChimeriVax-JE): phase 2 clinical trials for safety and immunogenicity, effect of vaccine dose and schedule, and memory response to challenge with inactivated Japanese encephalitis antigen","volume":"188","author":"Monath","year":"2003","journal-title":"J Infect Dis"},{"key":"10.1016\/j.coviro.2020.05.002_bib0225","doi-asserted-by":"crossref","first-page":"12497","DOI":"10.1128\/JVI.78.22.12497-12507.2004","article-title":"ChimeriVax-West Nile virus live-attenuated vaccine: preclinical evaluation of safety, immunogenicity, and efficacy","volume":"78","author":"Arroyo","year":"2004","journal-title":"J Virol"},{"key":"10.1016\/j.coviro.2020.05.002_bib0230","doi-asserted-by":"crossref","first-page":"13206","DOI":"10.1038\/s41598-018-31375-9","article-title":"Chimeric yellow fever 17D-Zika virus (ChimeriVax-Zika) as a live-attenuated Zika virus vaccine","volume":"8","author":"Giel-Moloney","year":"2018","journal-title":"Sci Rep"},{"key":"10.1016\/j.coviro.2020.05.002_bib0235","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1038\/s41541-018-0092-2","article-title":"A yellow fever-Zika chimeric virus vaccine candidate protects against Zika infection and congenital malformations in mice","volume":"3","author":"Kum","year":"2018","journal-title":"NPJ Vaccines"},{"key":"10.1016\/j.coviro.2020.05.002_bib0240","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1080\/22221751.2020.1730709","article-title":"A chimeric yellow fever-Zika virus vaccine candidate fully protects against yellow fever virus infection in mice","volume":"9","author":"Kum","year":"2020","journal-title":"Emerg Microbes Infect"},{"key":"10.1016\/j.coviro.2020.05.002_bib0245","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aax7888","article-title":"A recombinant platform for flavivirus vaccines and diagnostics using chimeras of a new insect-specific virus","volume":"11","author":"Hobson-Peters","year":"2019","journal-title":"Sci Transl Med"},{"key":"10.1016\/j.coviro.2020.05.002_bib0250","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.antiviral.2010.02.313","article-title":"A high-throughput assay using dengue-1 virus-like particles for drug discovery","volume":"86","author":"Qing","year":"2010","journal-title":"Antiviral Res"},{"key":"10.1016\/j.coviro.2020.05.002_bib0255","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00834-17","article-title":"Development of virus-like-particle vaccine and reporter assay for Zika virus","volume":"91","author":"Garg","year":"2017","journal-title":"J Virol"},{"key":"10.1016\/j.coviro.2020.05.002_bib0260","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1126\/science.aah6157","article-title":"Protective efficacy of multiple vaccine platforms against Zika virus challenge in rhesus monkeys","volume":"353","author":"Abbink","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.coviro.2020.05.002_bib0265","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01722-17","article-title":"Recombinant chimpanzee adenovirus vaccine AdC7-M\/E protects against Zika virus infection and testis damage","volume":"92","author":"Xu","year":"2018","journal-title":"J Virol"},{"key":"10.1016\/j.coviro.2020.05.002_bib0270","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0202820","article-title":"Adenoviral vector type 26 encoding Zika virus (ZIKV) M-Env antigen induces humoral and cellular immune responses and protects mice and nonhuman primates against ZIKV challenge","volume":"13","author":"Cox","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.coviro.2020.05.002_bib0275","doi-asserted-by":"crossref","first-page":"3023","DOI":"10.4049\/jimmunol.1602180","article-title":"Cutting edge: innate immune augmenting vesicular stomatitis virus expressing Zika virus proteins confers protective immunity","volume":"198","author":"Betancourt","year":"2017","journal-title":"J Immunol"},{"key":"10.1016\/j.coviro.2020.05.002_bib0280","article-title":"A Zika virus vaccine expressing premembrane-envelope-NS1 polyprotein","volume":"9","author":"Li","year":"2018","journal-title":"Nat Commun"},{"key":"10.1016\/j.coviro.2020.05.002_bib0285","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01485-18","article-title":"A measles virus-based vaccine candidate mediates protection against Zika virus in an allogeneic mouse pregnancy model","volume":"93","author":"N\u00fcrnberger","year":"2019","journal-title":"J Virol"},{"key":"10.1016\/j.coviro.2020.05.002_bib0290","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1158\/2326-6066.CIR-14-0015","article-title":"Oncolytic viruses and their application to cancer immunotherapy","volume":"2","author":"Chiocca","year":"2014","journal-title":"Cancer Immunol Res"},{"key":"10.1016\/j.coviro.2020.05.002_bib0295","doi-asserted-by":"crossref","first-page":"2843","DOI":"10.1084\/jem.20171093","article-title":"Zika virus has oncolytic activity against glioblastoma stem cells","volume":"214","author":"Zhu","year":"2017","journal-title":"J Exp Med"},{"key":"10.1016\/j.coviro.2020.05.002_bib0300","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01683-18","article-title":"Treatment of human glioblastoma with a live attenuated Zika virus vaccine candidate","volume":"9","author":"Chen","year":"2018","journal-title":"mBio"},{"key":"10.1016\/j.coviro.2020.05.002_bib0305","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/j.stem.2016.02.016","article-title":"Zika virus infects human cortical neural progenitors and attenuates their growth","volume":"18","author":"Tang","year":"2016","journal-title":"Cell Stem Cell"},{"key":"10.1016\/j.coviro.2020.05.002_bib0310","doi-asserted-by":"crossref","first-page":"2064","DOI":"10.1016\/j.ymthe.2019.10.004","article-title":"Repurposing an HIV drug for Zika virus therapy","volume":"27","author":"Xie","year":"2019","journal-title":"Mol Ther"},{"key":"10.1016\/j.coviro.2020.05.002_bib0315","doi-asserted-by":"crossref","first-page":"S945","DOI":"10.1093\/infdis\/jix406","article-title":"Small molecules and antibodies for Zika therapy","volume":"216","author":"Xie","year":"2017","journal-title":"J Infect Dis"},{"key":"10.1016\/j.coviro.2020.05.002_bib0320","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.coviro.2019.01.005","article-title":"Strategies for Zika drug discovery","volume":"35","author":"Zou","year":"2019","journal-title":"Curr Opin Virol"},{"key":"10.1016\/j.coviro.2020.05.002_bib0325","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.chom.2016.07.004","article-title":"A screen of FDA-approved drugs for inhibitors of Zika virus infection","volume":"20","author":"Barrows","year":"2016","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.coviro.2020.05.002_bib0330","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nm.4184","article-title":"Identification of small-molecule inhibitors of Zika virus infection and induced neural cell death via a drug repurposing screen","volume":"22","author":"Xu","year":"2016","journal-title":"Nat Med"},{"key":"10.1016\/j.coviro.2020.05.002_bib0335","doi-asserted-by":"crossref","DOI":"10.3390\/v12010061","article-title":"Novel approach for insertion of heterologous sequences into full-length ZIKV genome results in superior level of gene expression and insert stability","volume":"12","author":"Volkova","year":"2020","journal-title":"Viruses"},{"key":"10.1016\/j.coviro.2020.05.002_bib0340","doi-asserted-by":"crossref","first-page":"20435","DOI":"10.1073\/pnas.0907010106","article-title":"An adenosine nucleoside inhibitor of dengue virus","volume":"106","author":"Yin","year":"2009","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.coviro.2020.05.002_bib0345","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.ebiom.2017.03.006","article-title":"A rapid Zika diagnostic assay to measure neutralizing antibodies in patients","volume":"17","author":"Shan","year":"2017","journal-title":"EBioMedicine"},{"key":"10.1016\/j.coviro.2020.05.002_bib0350","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.tvjl.2012.08.019","article-title":"Review of diagnostic plaque reduction neutralization tests for flavivirus infection","volume":"195","author":"Maeda","year":"2013","journal-title":"Vet J"},{"key":"10.1016\/j.coviro.2020.05.002_bib0355","article-title":"A GFP reporter MR766-based flow cytometry neutralization test for rapid detection of Zika virus-neutralizing antibodies in serum specimens","volume":"7","author":"Frumence","year":"2019","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.coviro.2020.05.002_bib0360","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.antiviral.2017.12.017","article-title":"Development of a replicon cell line-based high throughput antiviral assay for screening inhibitors of Zika virus","volume":"150","author":"Li","year":"2018","journal-title":"Antiviral Res"},{"key":"10.1016\/j.coviro.2020.05.002_bib0365","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.virol.2005.10.030","article-title":"A rapid and quantitative assay for measuring antibody-mediated neutralization of West Nile virus infection","volume":"346","author":"Pierson","year":"2006","journal-title":"Virology"},{"key":"10.1016\/j.coviro.2020.05.002_bib0370","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1016\/j.celrep.2016.07.049","article-title":"Broadly neutralizing activity of Zika virus-immune sera identifies a single viral serotype","volume":"16","author":"Dowd","year":"2016","journal-title":"Cell Rep"},{"key":"10.1016\/j.coviro.2020.05.002_bib0375","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/S0140-6736(17)33105-7","article-title":"Safety, tolerability, and immunogenicity of two Zika virus DNA vaccine candidates in healthy adults: randomised, open-label, phase 1 clinical trials","volume":"391","author":"Gaudinski","year":"2018","journal-title":"Lancet"},{"key":"10.1016\/j.coviro.2020.05.002_bib0380","doi-asserted-by":"crossref","first-page":"16624","DOI":"10.1038\/s41598-018-34865-y","article-title":"High-throughput neutralization assay for multiple flaviviruses based on single-round infectious particles using dengue virus type 1 reporter replicon","volume":"8","author":"Matsuda","year":"2018","journal-title":"Sci Rep"},{"key":"10.1016\/j.coviro.2020.05.002_bib0385","article-title":"Safety and immunogenicity of an anti-Zika virus DNA vaccine - preliminary report","author":"Tebas","year":"2017","journal-title":"N Engl J Med"},{"key":"10.1016\/j.coviro.2020.05.002_bib0390","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/S0140-6736(17)33106-9","article-title":"Preliminary aggregate safety and immunogenicity results from three trials of a purified inactivated Zika virus vaccine candidate: phase 1, randomised, double-blind, placebo-controlled clinical trials","volume":"391","author":"Modjarrad","year":"2018","journal-title":"Lancet"},{"key":"10.1016\/j.coviro.2020.05.002_bib0395","unstructured":"World Health Organization. WHO vaccine pipeline tracker. https:\/\/www.who.int\/immunization\/research\/vaccine_pipeline_tracker_spreadsheet\/en\/. Accessed March 2020."}],"container-title":["Current Opinion in Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1879625720300237?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1879625720300237?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T10:03:47Z","timestamp":1761818627000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1879625720300237"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10]]},"references-count":79,"alternative-id":["S1879625720300237"],"URL":"https:\/\/doi.org\/10.1016\/j.coviro.2020.05.002","relation":{},"ISSN":["1879-6257"],"issn-type":[{"type":"print","value":"1879-6257"}],"subject":[],"published":{"date-parts":[[2020,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Reverse genetic approaches for the development of Zika vaccines and therapeutics","name":"articletitle","label":"Article Title"},{"value":"Current Opinion in Virology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.coviro.2020.05.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}