{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:29:58Z","timestamp":1787506198666,"version":"build-2736575974"},"reference-count":66,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,7,23]],"date-time":"2022-07-23T00:00:00Z","timestamp":1658534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008476","name":"University of California, Irvine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008476","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01DA048815"],"award-info":[{"award-number":["R01DA048815"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R03AG063264"],"award-info":[{"award-number":["R03AG063264"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21NS109918"],"award-info":[{"award-number":["R21NS109918"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cell Reports Methods"],"published-print":{"date-parts":[[2022,8]]},"DOI":"10.1016\/j.crmeth.2022.100276","type":"journal-article","created":{"date-parts":[[2022,8,22]],"date-time":"2022-08-22T10:42:59Z","timestamp":1661164979000},"page":"100276","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"title":["A genetic tool for the longitudinal study of a subset of post-inflammatory reactive astrocytes"],"prefix":"10.1016","volume":"2","author":[{"given":"William","family":"Agnew-Svoboda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Ubina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoe","family":"Figueroa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiu-Cheung","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward A.","family":"Vizcarra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan","family":"Roebini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma H.","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd A.","family":"Fiacco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1867-4439","authenticated-orcid":false,"given":"Martin M.","family":"Riccomagno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.crmeth.2022.100276_bib1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/s41593-017-0033-9","article-title":"The diversity and disparity of the glial scar","volume":"21","author":"Adams","year":"2018","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.crmeth.2022.100276_bib2","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.neulet.2013.12.030","article-title":"Heterogeneity of reactive astrocytes","volume":"565","author":"Anderson","year":"2014","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.crmeth.2022.100276_bib3","doi-asserted-by":"crossref","first-page":"7025","DOI":"10.1523\/JNEUROSCI.1954-08.2008","article-title":"A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping","volume":"28","author":"Atasoy","year":"2008","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.crmeth.2022.100276_bib4","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1038\/ng1228","article-title":"Cryptic boundaries in roof plate and choroid plexus identified by intersectional gene activation","volume":"35","author":"Awatramani","year":"2003","journal-title":"Nat. Genet."},{"key":"10.1016\/j.crmeth.2022.100276_bib5","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1002\/glia.20394","article-title":"Functional changes in astroglial cells in epilepsy","volume":"54","author":"Binder","year":"2006","journal-title":"Glia"},{"key":"10.1016\/j.crmeth.2022.100276_bib6","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1002\/dvg.20826","article-title":"Highly-efficient, fluorescent, locus directed cre and FlpO deleter mice on a pure C57BL\/6N genetic background","volume":"50","author":"Birling","year":"2012","journal-title":"Genesis"},{"key":"10.1016\/j.crmeth.2022.100276_bib7","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1084\/jem.20041918","article-title":"Inhibition of astroglial nuclear factor \u03baB reduces inflammation and improves functional recovery after spinal cord injury","volume":"202","author":"Brambilla","year":"2005","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.crmeth.2022.100276_bib8","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.neuron.2013.12.034","article-title":"Reactive gliosis and the multicellular response to CNS damage and disease","volume":"81","author":"Burda","year":"2014","journal-title":"Neuron"},{"key":"10.1016\/j.crmeth.2022.100276_bib9","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1523\/JNEUROSCI.4178-07.2008","article-title":"A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function","volume":"28","author":"Cahoy","year":"2008","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.crmeth.2022.100276_bib10","doi-asserted-by":"crossref","first-page":"3166","DOI":"10.1038\/nprot.2006.450","article-title":"Stereotaxic gene delivery in the rodent brain","volume":"1","author":"Cetin","year":"2006","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.crmeth.2022.100276_bib11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1101\/cshperspect.a022434","article-title":"Role of astrocytes in epilpesy","volume":"5","author":"Coulter","year":"2015","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"10.1016\/j.crmeth.2022.100276_bib12","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/j.cell.2018.06.035","article-title":"A suite of transgenic driver and reporter mouse lines with enhanced brain-cell-type targeting and functionality","volume":"174","author":"Daigle","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.crmeth.2022.100276_bib13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1126\/scitranslmed.aaw8546","article-title":"Astrocyte molecular signatures in Huntington\u2019s disease","volume":"11","author":"Diaz-Castro","year":"2019","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.crmeth.2022.100276_bib14","doi-asserted-by":"crossref","first-page":"109508","DOI":"10.1016\/j.celrep.2021.109508","article-title":"Molecular and functional properties of cortical astrocytes during peripherally induced neuroinflammation","volume":"36","author":"Diaz-Castro","year":"2021","journal-title":"Cell Rep."},{"key":"10.1016\/j.crmeth.2022.100276_bib15","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.1017\/S0031182021001013","article-title":"Congenital toxoplasmosis in humans: an update of worldwide rate of congenital infections","volume":"148","author":"Dubey","year":"2021","journal-title":"Parasitology"},{"key":"10.1016\/j.crmeth.2022.100276_bib16","series-title":"Mobile DNA III","first-page":"119","article-title":"Cre recombinase","author":"Van Duyne","year":"2015"},{"key":"10.1016\/j.crmeth.2022.100276_bib17","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0006-8993(71)90668-8","article-title":"An acidic protein isolated from fibrous astrocytes","volume":"28","author":"Eng","year":"1971","journal-title":"Brain Res."},{"key":"10.1016\/j.crmeth.2022.100276_bib18","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1007\/s00281-009-0177-0","article-title":"The blood\u2013brain and the blood\u2013cerebrospinal fluid barriers: function and dysfunction","volume":"31","author":"Engelhardt","year":"2009","journal-title":"Semin. Immunopathol."},{"key":"10.1016\/j.crmeth.2022.100276_bib19","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1038\/s41593-020-00783-4","article-title":"Reactive astrocyte nomenclature, definitions, and future directions","volume":"24","author":"Escartin","year":"2021","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.crmeth.2022.100276_bib20","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/j.cell.2020.01.001","article-title":"All-optical electrophysiology reveals the role of lateral inhibition in sensory processing in cortical layer 1","volume":"180","author":"Fan","year":"2020","journal-title":"Cell"},{"key":"10.1016\/j.crmeth.2022.100276_bib21","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1038\/nmeth.2996","article-title":"Targeting cells with single vectors using multiple-feature Boolean logic","volume":"11","author":"Fenno","year":"2014","journal-title":"Nat. Methods"},{"key":"10.1016\/j.crmeth.2022.100276_bib22","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/j.neuron.2020.06.003","article-title":"Comprehensive dual- and triple-feature intersectional single-vector delivery of diverse functional payloads to cells of behaving mammals","volume":"107","author":"Fenno","year":"2020","journal-title":"Neuron"},{"key":"10.1016\/j.crmeth.2022.100276_bib23","first-page":"1","article-title":"Monitoring astrocyte reactivity and proliferation in\u00a0vitro under ischemic-like conditions","volume":"2017","author":"Ferrer-Acosta","year":"2017","journal-title":"J.\u00a0Vis. Exp."},{"key":"10.1016\/j.crmeth.2022.100276_bib24","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1002\/glia.23140","article-title":"Anti-ACSA-2 defines a novel monoclonal antibody for prospective isolation of living neonatal and adult astrocytes","volume":"65","author":"Kantzer","year":"2017","journal-title":"Glia"},{"key":"10.1016\/j.crmeth.2022.100276_bib25","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1038\/s41593-020-0624-8","article-title":"Disease-associated astrocytes in Alzheimer\u2019s disease and aging","volume":"23","author":"Habib","year":"2020","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.crmeth.2022.100276_bib26","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1038\/s41593-021-00905-6","article-title":"Neuroinflammatory astrocyte subtypes in the mouse brain","volume":"24","author":"Hasel","year":"2021","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.crmeth.2022.100276_bib27","doi-asserted-by":"crossref","first-page":"38276","DOI":"10.1038\/srep38276","article-title":"Design and mechanistic insight into ultrafast calcium indicators for monitoring intracellular calcium dynamics","volume":"6","author":"Helassa","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.crmeth.2022.100276_bib28","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1002\/jnr.22012","article-title":"Intrastriatal lipopolysaccharide injection induces Parkinsonism in C57\/B6 mice","volume":"87","author":"Hunter","year":"2009","journal-title":"J.\u00a0Neurosci. Res."},{"key":"10.1016\/j.crmeth.2022.100276_bib29","doi-asserted-by":"crossref","first-page":"2583","DOI":"10.1007\/s11064-015-1581-6","article-title":"The glymphatic system: a beginner\u2019s guide","volume":"40","author":"Jessen","year":"2015","journal-title":"Neurochem. Res."},{"key":"10.1016\/j.crmeth.2022.100276_bib30","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1002\/glia.23759","article-title":"Ablation of reactive astrocytes exacerbates disease pathology in a model of Alzheimer\u2019s disease","volume":"68","author":"Katsouri","year":"2020","journal-title":"Glia"},{"key":"10.1016\/j.crmeth.2022.100276_bib31","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1159\/000111560","article-title":"Opposing roles for reactive astrocytes following traumatic brain injury","volume":"16","author":"Laird","year":"2008","journal-title":"Neurosignals"},{"key":"10.1016\/j.crmeth.2022.100276_bib32","doi-asserted-by":"crossref","first-page":"4335","DOI":"10.1038\/s41467-021-24624-5","article-title":"Extensive transcriptional and chromatin changes underlie astrocyte maturation in\u00a0vivo and in culture","volume":"12","author":"Lattke","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.crmeth.2022.100276_bib33","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1002\/glia.24191","article-title":"The role of astrocyte structural plasticity in regulating neural circuit function and behavior","volume":"70","author":"Lawal","year":"2022","journal-title":"Glia"},{"key":"10.1016\/j.crmeth.2022.100276_bib67","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1002\/glia.20622","article-title":"GFAP promoter elements required for region-specific and astrocyte-specific expression","volume":"56","author":"Lee","year":"2008","journal-title":"Glia"},{"key":"10.1016\/j.crmeth.2022.100276_bib34","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1038\/nature21029","article-title":"Neurotoxic reactive astrocytes are induced by activated microglia","volume":"541","author":"Liddelow","year":"2017","journal-title":"Nature"},{"key":"10.1016\/j.crmeth.2022.100276_bib35","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1101\/gr.749203","article-title":"A highly efficient recombineering-based method for generating conditional knockout mutations","volume":"13","author":"Liu","year":"2003","journal-title":"Genome Res."},{"key":"10.1016\/j.crmeth.2022.100276_bib36","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1038\/nn.2467","article-title":"A robust and high-throughput Cre reporting and characterization system for the whole mouse brain","volume":"13","author":"Madisen","year":"2010","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.crmeth.2022.100276_bib37","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1007\/s00424-015-1765-6","article-title":"Volume-regulated anion channel--a frenemy within the brain","volume":"468","author":"Mongin","year":"2016","journal-title":"Pflugers Arch."},{"key":"10.1016\/j.crmeth.2022.100276_bib39","doi-asserted-by":"crossref","first-page":"155","DOI":"10.2174\/1570159X14666151204122017","article-title":"LPS-Induced murine neuroinflammation model: main features and suitability for pre-clinical assessment of nutraceuticals","volume":"14","author":"Nava Catorce","year":"2016","journal-title":"Curr. Neuropharmacol."},{"key":"10.1016\/j.crmeth.2022.100276_bib40","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.1128\/IAI.01314-09","article-title":"CCR7-dependent immunity during acute Toxoplasma gondii infection","volume":"78","author":"Noor","year":"2010","journal-title":"Infect. Immun."},{"key":"10.1016\/j.crmeth.2022.100276_bib41","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1038\/nm1425","article-title":"Conditional ablation of Stat3 or Socs3 discloses a dual role for reactive astrocytes after spinal cord injury","volume":"12","author":"Okada","year":"2006","journal-title":"Nat. Med."},{"key":"10.1016\/j.crmeth.2022.100276_bib42","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.neuroscience.2015.03.063","article-title":"Astrocytic mGluR5 and the tripartite synapse","volume":"323","author":"Panatier","year":"2016","journal-title":"Neuroscience"},{"key":"10.1016\/j.crmeth.2022.100276_bib43","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1186\/s40478-021-01180-z","article-title":"Blocking microglial activation of reactive astrocytes is neuroprotective in models of Alzheimer\u2019s disease","volume":"9","author":"Park","year":"2021","journal-title":"Acta Neuropathol. Commun."},{"key":"10.1016\/j.crmeth.2022.100276_bib44","doi-asserted-by":"crossref","first-page":"7034","DOI":"10.1128\/JVI.77.12.7034-7040.2003","article-title":"Intraventricular brain injection of adeno-associated virus type 1 (AAV1) in neonatal mice results in complementary patterns of neuronal transduction to AAV2 and total long-term correction of storage lesions in the brains of beta-glucuronidase-deficient mi","volume":"77","author":"Passini","year":"2003","journal-title":"J.\u00a0Virol."},{"key":"10.1016\/j.crmeth.2022.100276_bib45","doi-asserted-by":"crossref","DOI":"10.3389\/fnmol.2019.00164","article-title":"Post-translational regulation of GLT-1 in neurological diseases and its potential as an effective therapeutic target","volume":"12","author":"Peterson","year":"2019","journal-title":"Front. Mol. Neurosci."},{"key":"10.1016\/j.crmeth.2022.100276_bib46","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1002\/glia.20876","article-title":"Conditional deletion of \u03b21-integrin in astroglia causes partial reactive gliosis","volume":"57","author":"Robel","year":"2009","journal-title":"Glia"},{"key":"10.1016\/j.crmeth.2022.100276_bib47","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1073\/pnas.0712168105","article-title":"A chemical method for fast and sensitive detection of DNA synthesis in\u00a0vivo","volume":"105","author":"Salic","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.crmeth.2022.100276_bib48","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.crmeth.2022.100276_bib49","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1016\/j.celrep.2017.04.047","article-title":"Transformation of astrocytes to a neuroprotective phenotype by microglia via P2Y1Receptor downregulation","volume":"19","author":"Shinozaki","year":"2017","journal-title":"Cell Rep."},{"key":"10.1016\/j.crmeth.2022.100276_bib50","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1038\/nbt811","article-title":"A directional strategy for monitoring Cre-mediated recombination at the cellular level in the mouse","volume":"21","author":"Schn\u00fctgen","year":"2003","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.crmeth.2022.100276_bib51","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/j.tins.2009.08.002","article-title":"Molecular dissection of reactive astrogliosis and glial scar formation","volume":"32","author":"Sofroniew","year":"2009","journal-title":"Trends Neurosci."},{"key":"10.1016\/j.crmeth.2022.100276_bib52","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a020420","article-title":"Astrogliosis","volume":"7","author":"Sofroniew","year":"2015","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"10.1016\/j.crmeth.2022.100276_bib53","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1016\/j.it.2020.07.004","article-title":"Astrocyte reactivity : subtypes , states , and functions in CNS innate immunity","volume":"41","author":"Sofroniew","year":"2020","journal-title":"Trends Immunol."},{"key":"10.1016\/j.crmeth.2022.100276_bib54","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s00401-009-0619-8","article-title":"Astrocytes: Biology and pathology","volume":"119","author":"Sofroniew","year":"2010","journal-title":"Acta Neuropathol."},{"key":"10.1016\/j.crmeth.2022.100276_bib55","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1016\/j.neuron.2016.11.030","article-title":"New transgenic mouse lines for selectively targeting astrocytes and studying calcium signals in astrocyte processes in\u00a0situ and in\u00a0vivo","volume":"92","author":"Srinivasan","year":"2016","journal-title":"Neuron"},{"key":"10.1016\/j.crmeth.2022.100276_bib56","doi-asserted-by":"crossref","DOI":"10.1002\/9780471729259.mc14d05s29","article-title":"Gene transfer to the CNS using recombinant adeno-associated virus","author":"Stoica","year":"2013","journal-title":"Curr. Protoc. Microbiol."},{"key":"10.1016\/j.crmeth.2022.100276_bib57","doi-asserted-by":"crossref","first-page":"jcs257212","DOI":"10.1242\/jcs.257212","article-title":"ExBoX \u2013 a simple Boolean exclusion strategy to drive expression in neurons","volume":"134","author":"Ubina","year":"2021","journal-title":"J.\u00a0Cell Sci."},{"key":"10.1016\/j.crmeth.2022.100276_bib58","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jnr.24634","article-title":"Parallel astrocyte calcium signaling modulates olfactory bulb responses","volume":"98","author":"Ung","year":"2020","journal-title":"J.\u00a0Neurosci. Res."},{"key":"10.1016\/j.crmeth.2022.100276_bib59","doi-asserted-by":"crossref","DOI":"10.1101\/pdb.prot087387","article-title":"Analysis of apoptosis and necroptosis by fluorescence-activated cell sorting","volume":"2016","author":"Wallberg","year":"2016","journal-title":"Cold Spring Harb. Protoc."},{"key":"10.1016\/j.crmeth.2022.100276_bib60","doi-asserted-by":"crossref","first-page":"12870","DOI":"10.1523\/JNEUROSCI.2121-13.2013","article-title":"Glial scar borders are formed by newly proliferated, elongated astrocytes that interact to corral inflammatory and fibrotic cells via STAT3-dependent mechanisms after spinal cord injury","volume":"33","author":"Wanner","year":"2013","journal-title":"J.\u00a0Neurosci."},{"key":"10.1016\/j.crmeth.2022.100276_bib61","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1038\/s41586-020-1999-0","article-title":"MAFG-driven astrocytes promote CNS inflammation","volume":"578","author":"Wheeler","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.crmeth.2022.100276_bib62","doi-asserted-by":"crossref","first-page":"17513","DOI":"10.1073\/pnas.0602841103","article-title":"Redefining the concept of reactive astrocytes as cells that remain within their unique domains upon reaction to injury","volume":"103","author":"Wilhelmsson","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.crmeth.2022.100276_bib63","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.pt.2017.04.001","article-title":"Brains and brawn: Toxoplasma infections of the central nervous system and skeletal muscle","volume":"33","author":"Wohlfert","year":"2017","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.crmeth.2022.100276_bib64","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/j.neuron.2019.03.029","article-title":"Glutamate-releasing SWELL1 channel in astrocytes modulates synaptic transmission and promotes brain damage in stroke","volume":"102","author":"Yang","year":"2019","journal-title":"Neuron"},{"key":"10.1016\/j.crmeth.2022.100276_bib65","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1038\/s41591-018-0051-5","article-title":"Block of A1 astrocyte conversion by microglia is neuroprotective in models of Parkinson\u2019s disease","volume":"24","author":"Yun","year":"2018","journal-title":"Nat. Med."},{"key":"10.1016\/j.crmeth.2022.100276_bib66","doi-asserted-by":"crossref","first-page":"6391","DOI":"10.1523\/JNEUROSCI.6221-11.2012","article-title":"Genomic analysis of reactive astrogliosis","volume":"32","author":"Zamanian","year":"2012","journal-title":"J.\u00a0Neurosci."}],"container-title":["Cell Reports Methods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2667237522001539?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2667237522001539?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T22:57:59Z","timestamp":1762642679000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2667237522001539"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8]]},"references-count":66,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["S2667237522001539"],"URL":"https:\/\/doi.org\/10.1016\/j.crmeth.2022.100276","relation":{},"ISSN":["2667-2375"],"issn-type":[{"value":"2667-2375","type":"print"}],"subject":[],"published":{"date-parts":[[2022,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A genetic tool for the longitudinal study of a subset of post-inflammatory reactive astrocytes","name":"articletitle","label":"Article Title"},{"value":"Cell Reports Methods","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.crmeth.2022.100276","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Author(s).","name":"copyright","label":"Copyright"}],"article-number":"100276"}}