{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T10:33:42Z","timestamp":1793097222752,"version":"4.5.0"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":155,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministry of Health of the Czech Republic","doi-asserted-by":"publisher","award":["NU21\\u201303-00421"],"award-info":[{"award-number":["NU21\\u201303-00421"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100022597","name":"Biofyzik\u00e1ln\u00ed \u00dastav, Akademie V\u011bd \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["RVO: 68081707"],"award-info":[{"award-number":["RVO: 68081707"]}],"id":[{"id":"10.13039\/501100022597","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Current Research in Toxicology"],"published-print":{"date-parts":[[2026]]},"DOI":"10.1016\/j.crtox.2026.100306","type":"journal-article","created":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T15:46:47Z","timestamp":1780760807000},"page":"100306","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Downregulation of lactosylceramide synthases B4GALT5 or B4GALT6 alters glycosphingolipid profiles and enhances oxaliplatin sensitivity in colon cancer cells"],"prefix":"10.1016","volume":"11","author":[{"given":"Barbora","family":"Lujka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tereza","family":"\u0160o\u0161ol\u00edkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerardo","family":"V\u00e1zquez-G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ondrej","family":"Kov\u00e1\u010d","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslav","family":"Machala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alena Hyr\u0161lov\u00e1","family":"Vaculov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Vondr\u00e1\u010dek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.crtox.2026.100306_bb0005","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1038\/sj.bjc.6602215","article-title":"Molecular mechanisms of action and prediction of response to oxaliplatin in colorectal cancer cells","volume":"91","author":"Arango","year":"2004","journal-title":"Br. J. Cancer"},{"key":"10.1016\/j.crtox.2026.100306_bb0010","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1038\/s41420-022-01085-3","article-title":"ATF6 prevents DNA damage and cell death in colon cancer cells undergoing ER stress","volume":"8","author":"Benedetti","year":"2022","journal-title":"Cell Death Discov."},{"key":"10.1016\/j.crtox.2026.100306_bb0015","first-page":"229","article-title":"Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"74","author":"Bray","year":"2024","journal-title":"CA Cancer J. Clin."},{"key":"10.1016\/j.crtox.2026.100306_bb0020","doi-asserted-by":"crossref","first-page":"7148","DOI":"10.1016\/S0021-9258(19)50550-6","article-title":"Purification of uridine diphosphate-galactose:glucosyl ceramide, beta 1-4 galactosyltransferase from human kidney","volume":"267","author":"Chatterjee","year":"1992","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.crtox.2026.100306_bb0025","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0063726","article-title":"Use of a glycolipid inhibitor to ameliorate renal cancer in a mouse model","volume":"8","author":"Chatterjee","year":"2013","journal-title":"PloS One"},{"key":"10.1016\/j.crtox.2026.100306_bb0030","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.bbrc.2018.11.149","article-title":"Lactosylceramide synthase \u03b2-1,4-GalT-V: a novel target for the diagnosis and therapy of human colorectal cancer","volume":"508","author":"Chatterjee","year":"2019","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.crtox.2026.100306_bb0035","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1053\/j.gastro.2018.07.028","article-title":"Activated ATF6 induces intestinal dysbiosis and innate immune response to promote colorectal tumorigenesis","volume":"155","author":"Coleman","year":"2018","journal-title":"Gastroenterology"},{"key":"10.1016\/j.crtox.2026.100306_bb0040","doi-asserted-by":"crossref","DOI":"10.3390\/biom11010062","article-title":"Deciphering the importance of glycosphingolipids on cellular and molecular mechanisms associated with epithelial-to-mesenchymal transition in cancer","volume":"11","author":"Cumin","year":"2021","journal-title":"Biomolecules"},{"key":"10.1016\/j.crtox.2026.100306_bb0045","doi-asserted-by":"crossref","first-page":"6338","DOI":"10.1111\/febs.12559","article-title":"Glycosphingolipids: synthesis and functions","volume":"280","author":"D'Angelo","year":"2013","journal-title":"FEBS J."},{"key":"10.1016\/j.crtox.2026.100306_bb0050","doi-asserted-by":"crossref","DOI":"10.1038\/srep28973","article-title":"CRISP-ID: decoding CRISPR mediated indels by sanger sequencing","volume":"6","author":"Dehairs","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.crtox.2026.100306_bb0055","first-page":"1020","article-title":"N,N-dimethylformamide-induced alteration of cell culture characteristics and loss of tumorigenicity in cultured human colon carcinoma cells","volume":"39","author":"Dexter","year":"1979","journal-title":"Cancer Res."},{"key":"10.1016\/j.crtox.2026.100306_bb0060","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1053\/j.gastro.2011.04.055","article-title":"Oxaliplatin sensitizes human colon cancer cells to TRAIL through JNK-dependent phosphorylation of Bcl-xL","volume":"141","author":"El Fajoui","year":"2011","journal-title":"Gastroenterology"},{"key":"10.1016\/j.crtox.2026.100306_bb0065","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1111\/cas.14005","article-title":"New era of research on cancer-associated glycosphingolipids","volume":"110","author":"Furukawa","year":"2019","journal-title":"Cancer Sci."},{"key":"10.1016\/j.crtox.2026.100306_bb0070","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/S0889-8553(02)00057-2","article-title":"Modifiable risk factors for colon cancer","volume":"31","author":"Giovannucci","year":"2002","journal-title":"Gastroenterol. Clin. North Am."},{"key":"10.1016\/j.crtox.2026.100306_bb0075","doi-asserted-by":"crossref","first-page":"7461","DOI":"10.1073\/pnas.0902368106","article-title":"Specific glycosphingolipids mediate epithelial-to-mesenchymal transition of human and mouse epithelial cell lines","volume":"106","author":"Guan","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.crtox.2026.100306_bb0080","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1038\/nrm.2017.107","article-title":"Sphingolipids and their metabolism in physiology and disease","volume":"19","author":"Hannun","year":"2018","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.crtox.2026.100306_bb0085","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1080\/15548627.2023.2258701","article-title":"ER stress elicits non-canonical CASP8 (caspase 8) activation on autophagosomal membranes to induce apoptosis","volume":"20","author":"Hattori","year":"2024","journal-title":"Autophagy"},{"key":"10.1016\/j.crtox.2026.100306_bb0090","doi-asserted-by":"crossref","DOI":"10.1186\/s12929-018-0453-1","article-title":"A lifetime of stress: ATF6 in development and homeostasis","volume":"25","author":"Hillary","year":"2018","journal-title":"J. Biomed. Sci."},{"key":"10.1016\/j.crtox.2026.100306_bb0095","doi-asserted-by":"crossref","first-page":"60575","DOI":"10.18632\/oncotarget.11169","article-title":"Inhibition of glucosylceramide synthase eliminates the oncogenic function of p53 R273H mutant in the epithelial-mesenchymal transition and induced pluripotency of colon cancer cells","volume":"7","author":"Hosain","year":"2016","journal-title":"Oncotarget"},{"key":"10.1016\/j.crtox.2026.100306_bb0100","doi-asserted-by":"crossref","first-page":"5458","DOI":"10.1002\/jcp.26406","article-title":"Oxaliplatin resistance in colorectal cancer cells is mediated via activation of ABCG2 to alleviate ER stress induced apoptosis","volume":"233","author":"Hsu","year":"2018","journal-title":"J. Cell. Physiol."},{"key":"10.1016\/j.crtox.2026.100306_bb0105","doi-asserted-by":"crossref","DOI":"10.1155\/2011\/565316","article-title":"Apoptotic sphingolipid ceramide in cancer therapy","volume":"2011","author":"Huang","year":"2011","journal-title":"J Lipids"},{"key":"10.1016\/j.crtox.2026.100306_bb0110","doi-asserted-by":"crossref","first-page":"109201","DOI":"10.18632\/oncotarget.22648","article-title":"Inhibition of hepatocellular carcinoma growth by blockade of glycosphingolipid synthesis","volume":"8","author":"Jennemann","year":"2017","journal-title":"Oncotarget"},{"key":"10.1016\/j.crtox.2026.100306_bb0115","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.etap.2016.05.005","article-title":"Endoplasmic reticulum stress induces 5-fluorouracil resistance in human colon cancer cells","volume":"44","author":"Kim","year":"2016","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"10.1016\/j.crtox.2026.100306_bb0120","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1007\/s10719-010-9313-2","article-title":"Involvement of murine \u03b2-1,4-galactosyltransferase V in lactosylceramide biosynthesis","volume":"27","author":"Kumagai","year":"2010","journal-title":"Glycoconj. J."},{"key":"10.1016\/j.crtox.2026.100306_bb0125","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0141020","article-title":"Loss of PTEN facilitates rosiglitazone-mediated enhancement of platinum(IV) complex LA-12-induced apoptosis in colon cancer cells","volume":"10","author":"Laukov\u00e1","year":"2015","journal-title":"PloS One"},{"key":"10.1016\/j.crtox.2026.100306_bb0130","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1186\/s12885-022-10415-8","article-title":"Intense endoplasmic reticulum stress (ERS) \/ IRE1alpha enhanced oxaliplatin efficacy by decreased ABCC10 in colorectal cancer cells","volume":"22","author":"Liu","year":"2022","journal-title":"BMC Cancer"},{"key":"10.1016\/j.crtox.2026.100306_bb0135","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.crtox.2026.100306_bb0140","doi-asserted-by":"crossref","DOI":"10.3390\/ijms20236051","article-title":"Colon cancer and perturbations of the sphingolipid metabolism","volume":"20","author":"Machala","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.crtox.2026.100306_bb0145","doi-asserted-by":"crossref","DOI":"10.1016\/j.yexcr.2020.111860","article-title":"A role for ceramide glycosylation in resistance to oxaliplatin in colorectal cancer","volume":"388","author":"Madigan","year":"2020","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.crtox.2026.100306_bb0150","doi-asserted-by":"crossref","first-page":"6387","DOI":"10.1021\/cr2002917","article-title":"Sphingolipid and glycosphingolipid metabolic pathways in the era of sphingolipidomics","volume":"111","author":"Merrill","year":"2011","journal-title":"Chem. Rev."},{"key":"10.1016\/j.crtox.2026.100306_bb0155","doi-asserted-by":"crossref","first-page":"3632","DOI":"10.1002\/1873-3468.13863","article-title":"The role of sphingolipids in endoplasmic reticulum stress","volume":"594","author":"Park","year":"2020","journal-title":"FEBS Lett."},{"key":"10.1016\/j.crtox.2026.100306_bb0160","article-title":"Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors","volume":"1865","author":"Proch\u00e1zkov\u00e1","year":"2020","journal-title":"Biochim. Biophys. Acta Mol. Cell Biol. Lipids"},{"key":"10.1016\/j.crtox.2026.100306_bb0165","doi-asserted-by":"crossref","first-page":"292","DOI":"10.2174\/138945008783954943","article-title":"Structure and function of beta \u22121,4-galactosyltransferase","volume":"9","author":"Qasba","year":"2008","journal-title":"Curr. Drug Targets"},{"key":"10.1016\/j.crtox.2026.100306_bb0170","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.1158\/1535-7163.MCT-15-0171","article-title":"Inhibition of glucosylceramide synthase sensitizes head and neck cancer to cisplatin","volume":"14","author":"Roh","year":"2015","journal-title":"Mol. Cancer Ther."},{"key":"10.1016\/j.crtox.2026.100306_bb0175","doi-asserted-by":"crossref","first-page":"6457","DOI":"10.1074\/jbc.RA120.013090","article-title":"Inhibition of glycosphingolipid biosynthesis reverts multidrug resistance by differentially modulating ABC transporters in chronic myeloid leukemias","volume":"295","author":"Salustiano","year":"2020","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.crtox.2026.100306_bb0180","doi-asserted-by":"crossref","DOI":"10.3390\/biomedicines13030642","article-title":"Advances in targeted and chemotherapeutic strategies for colorectal cancer: current insights and future directions","volume":"13","author":"Shaham","year":"2025","journal-title":"Biomedicines"},{"key":"10.1016\/j.crtox.2026.100306_bb0185","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0051076","article-title":"Autophagy protects against oxaliplatin-induced cell death via ER stress and ROS in Caco-2 cells","volume":"7","author":"Shi","year":"2012","journal-title":"PloS One"},{"key":"10.1016\/j.crtox.2026.100306_bb0190","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.tips.2023.01.003","article-title":"Metastatic colorectal cancer: mechanisms and emerging therapeutics","volume":"44","author":"Shin","year":"2023","journal-title":"Trends Pharmacol. Sci."},{"key":"10.1016\/j.crtox.2026.100306_bb0195","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1093\/glycob\/cwu021","article-title":"Gene expression levels of beta4-galactosyltransferase 5 correlate with the tumorigenic potentials of B16-F10 mouse melanoma cells","volume":"24","author":"Shirane","year":"2014","journal-title":"Glycobiology"},{"key":"10.1016\/j.crtox.2026.100306_bb0200","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/febs.15107","article-title":"A guide to assessing endoplasmic reticulum homeostasis and stress in mammalian systems","volume":"287","author":"Sicari","year":"2020","journal-title":"FEBS J."},{"key":"10.1016\/j.crtox.2026.100306_bb0205","doi-asserted-by":"crossref","first-page":"8253","DOI":"10.18632\/oncotarget.6982","article-title":"Targeting glucosylceramide synthase upregulation reverts sorafenib resistance in experimental hepatocellular carcinoma","volume":"7","author":"Stefanovic","year":"2016","journal-title":"Oncotarget"},{"key":"10.1016\/j.crtox.2026.100306_bb0210","first-page":"1084","article-title":"beta1,4-galactosyltransferase V modulates breast cancer stem cells through Wnt\/beta-catenin signaling pathway","volume":"52","author":"Tang","year":"2020","journal-title":"Cancer Res. Treat."},{"key":"10.1016\/j.crtox.2026.100306_bb0215","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.yexcr.2015.05.012","article-title":"Targeting glucosylceramide synthase induction of cell surface globotriaosylceramide (Gb3) in acquired cisplatin-resistance of lung cancer and malignant pleural mesothelioma cells","volume":"336","author":"Tyler","year":"2015","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.crtox.2026.100306_bb0220","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1007\/s12185-014-1679-7","article-title":"Glucosylceramide synthase promotes Bcl-2 expression via the ERK signaling pathway in the K562\/A02 leukemia drug-resistant cell line","volume":"100","author":"Wang","year":"2014","journal-title":"Int. J. Hematol."},{"key":"10.1016\/j.crtox.2026.100306_bb0225","doi-asserted-by":"crossref","DOI":"10.1186\/s12885-018-4084-4","article-title":"The UDP-glucose ceramide glycosyltransferase (UGCG) and the link to multidrug resistance protein 1 (MDR1)","volume":"18","author":"Wegner","year":"2018","journal-title":"BMC Cancer"},{"key":"10.1016\/j.crtox.2026.100306_bb0230","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.1007545","article-title":"Lactosylceramide synthases encoded by B4galt5 and 6 genes are pivotal for neuronal generation and myelin formation in mice","volume":"14","author":"Yoshihara","year":"2018","journal-title":"PLoS Genet."},{"key":"10.1016\/j.crtox.2026.100306_bb0235","article-title":"The role of glycosphingolipids in immune cell functions","volume":"10","author":"Zhang","year":"2019","journal-title":"Front. Immunol."},{"key":"10.1016\/j.crtox.2026.100306_bb0240","doi-asserted-by":"crossref","DOI":"10.1038\/s41420-024-02110-3","article-title":"Endoplasmic reticulum stress-a key guardian in cancer","volume":"10","author":"Zhang","year":"2024","journal-title":"Cell Death Discov."},{"key":"10.1016\/j.crtox.2026.100306_bb0245","doi-asserted-by":"crossref","DOI":"10.1038\/cddis.2013.186","article-title":"B4GALT family mediates the multidrug resistance of human leukemia cells by regulating the hedgehog pathway and the expression of p-glycoprotein and multidrug resistance-associated protein 1","volume":"4","author":"Zhou","year":"2013","journal-title":"Cell Death Dis."}],"container-title":["Current Research in Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666027X26000277?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2666027X26000277?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T09:48:38Z","timestamp":1793094518000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2666027X26000277"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":49,"alternative-id":["S2666027X26000277"],"URL":"https:\/\/doi.org\/10.1016\/j.crtox.2026.100306","relation":{},"ISSN":["2666-027X"],"issn-type":[{"value":"2666-027X","type":"print"}],"subject":[],"published":{"date-parts":[[2026]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Downregulation of lactosylceramide synthases B4GALT5 or B4GALT6 alters glycosphingolipid profiles and enhances oxaliplatin sensitivity in colon cancer cells","name":"articletitle","label":"Article Title"},{"value":"Current Research in Toxicology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.crtox.2026.100306","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"100306"}}