{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T07:43:34Z","timestamp":1790149414828,"version":"4.0.1"},"reference-count":18,"publisher":"MathDoc\/Centre Mersenne","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    La culture in vitro d'anth\u00e8res de bl\u00e9 dur (\n                    <jats:italic>Triticum turgidum subsp. durum<\/jats:italic>\n                    (Desf.) Husn.) donne habituellement un tr\u00e8s fort taux d'albinisme chez les plantes r\u00e9g\u00e9n\u00e9r\u00e9es, ce qui limite consid\u00e9rablement son utilisation. Seules les m\u00e9thodes de culture in vitro d'ovaires immatures ou le croisement interg\u00e9n\u00e9rique avec le ma\u00efs permettent d'obtenir chez cette esp\u00e8ce des plantes vertes haplo\u00efdes et, par l\u00e0, des haplo\u00efdes doubl\u00e9s d'origine gynog\u00e9n\u00e9tique. La pr\u00e9sente note concerne une autre m\u00e9thode d'obtention d'haplo\u00efdes doubl\u00e9s, d'origine androg\u00e9n\u00e9tique : la culture in vitro de microspores isol\u00e9es. On montre que cette m\u00e9thode, associ\u00e9e \u00e0 des pr\u00e9traitements de quelques jours \u00e0 plusieurs semaines, au froid seul on au froid avec du mannitol, des \u00e9pis pr\u00e9lev\u00e9s, laiss\u00e9s dans leur gaine foliaire, et avant l'isolement des microspores, permet non seulement d'am\u00e9liorer l'embryogen\u00e8se, mais aussi, pour la premi\u00e8re fois, d'obtenir des quantit\u00e9s relativement importantes de plantes chlorophylliennes. Ainsi, tous pr\u00e9traitements et t\u00e9moin confondus, un total de 16\u2009490 embryons androg\u00e9n\u00e9tiques a \u00e9t\u00e9 obtenu \u00e0 partir de\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" overflow=\"scroll\">\n                      <mml:mn>17<\/mml:mn>\n                      <mml:mo>,<\/mml:mo>\n                      <mml:mn>4<\/mml:mn>\n                      <mml:mo>\u00d7<\/mml:mo>\n                      <mml:msup>\n                        <mml:mn>10<\/mml:mn>\n                        <mml:mn>6<\/mml:mn>\n                      <\/mml:msup>\n                    <\/mml:math>\n                    microspores de deux vari\u00e9t\u00e9s de bl\u00e9 dur, dont 9320 ont \u00e9t\u00e9 repiqu\u00e9s et ont donn\u00e9 150 plantes chlorophylliennes. Notamment, le pr\u00e9traitement au froid \u00e0 4\u2009\u00b0C de longue dur\u00e9e (cinq semaines) pourrait \u00eatre prometteur pour la r\u00e9g\u00e9n\u00e9ration chlorophyllienne chez le bl\u00e9 dur.\n                  <\/jats:p>","DOI":"10.1016\/j.crvi.2005.05.009","type":"journal-article","created":{"date-parts":[[2005,7,6]],"date-time":"2005-07-06T18:12:11Z","timestamp":1120673531000},"page":"713-723","source":"Crossref","is-referenced-by-count":15,"title":["Plantes chlorophylliennes de bl\u00e9 dur obtenues par culture de microspores isol\u00e9es : importance des pr\u00e9traitements"],"prefix":"10.5802","volume":"328","author":[{"given":"Zelikha","family":"Labbani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Richard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"De Buyser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Picard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3842","published-online":{"date-parts":[[2005,7,5]]},"reference":[{"key":"key2025102316270467202_1","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1111\/j.1439-0523.1987.tb01089.x","article-title":"\u2018Florin\u2019, a doubled haploid wheat variety developed by the anther culture method","volume":"98","author":"De Buyser, J.","year":"1987","unstructured":"[1] De Buyser, J.; Henry, Y.; Lonnet, P.; Hertzog, R.; Hespel, A. \u2018Florin\u2019, a doubled haploid wheat variety developed by the anther culture method, Plant Breed., Volume 98 (1987), pp. 53-56","journal-title":"Plant Breed."},{"key":"key2025102316270467202_2","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1139\/g99-002","article-title":"A cytological study of pre-treatments used to improve isolated microspore cultures of wheat (<span class=\"italique\">Triticum aestivum<\/span> L.) cv. Chris","volume":"42","author":"Hu, T.","year":"1999","unstructured":"[2] Hu, T.; Kasha, K.J. A cytological study of pre-treatments used to improve isolated microspore cultures of wheat (Triticum aestivum L.) cv. Chris, Genome, Volume 42 (1999), pp. 432-441","journal-title":"Genome"},{"key":"key2025102316270467202_3","first-page":"269","article-title":"Protocols for anther and microspore culture of barley","volume":"111","author":"Jahne-Gartner, A.","year":"1999","unstructured":"[3] Jahne-Gartner, A.; Lorz, H. Protocols for anther and microspore culture of barley, Methods Mol. Biol., Volume 111 (1999), pp. 269-279","journal-title":"Methods Mol. Biol."},{"key":"key2025102316270467202_4","unstructured":"[4] J. De Buyser, P. Touraine, A. Ambroise, E. Picard, Induction of androgenetic embryos and chlorophyllian plants of Triticum aestivum from isolated microspore culture, in: A.E. Slinkard (Ed.), Proc. 9th Int. Wheat Genetics Symposium, Saskatoon, Saskatchewan, Canada, vol. 3, 2\u20137 August 1998, pp. 175\u2013177"},{"key":"key2025102316270467202_5","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/BF00022568","article-title":"Influence of genotype and culture conditions on the production of embryos from anthers of tetraploid wheat (<span class=\"italique\">Triticum turgidum<\/span>)","volume":"65","author":"Ghaemi, M.","year":"1993","unstructured":"[5] Ghaemi, M.; Sarrafi, A.; Alibert, G. Influence of genotype and culture conditions on the production of embryos from anthers of tetraploid wheat (Triticum turgidum), Euphytica, Volume 65 (1993), pp. 81-85","journal-title":"Euphytica"},{"key":"key2025102316270467202_6","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1023\/A:1005765529154","article-title":"Embryo formation and regeneration in <span class=\"italique\">Triticum turgidum ssp. durum<\/span> anther culture","volume":"51","author":"Saidi, N.","year":"1997","unstructured":"[6] Saidi, N.; Cherkaoui, S.; Chlyah, A.; Chlyah, H. Embryo formation and regeneration in Triticum turgidum ssp. durum anther culture, Plant Cell Tissue Organ Cult., Volume 51 (1997), pp. 27-33","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"key2025102316270467202_7","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/s004970000043","article-title":"Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley (<span class=\"italique\">Hordeum vulgare<\/span> L.)","volume":"13","author":"Caredda, S.","year":"2000","unstructured":"[7] Caredda, S.; Doncoeur, C.; Devaux, P.; Sangwan, R.S.; Cl\u00e9ment, C. Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley (Hordeum vulgare L.), Sex Plant Reprod., Volume 13 (2000), pp. 95-104","journal-title":"Sex Plant Reprod."},{"key":"key2025102316270467202_8","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1007\/BF00222448","article-title":"Durum wheat haploid production using maize wide-crossing","volume":"89","author":"O'Donoughue, L.S.","year":"1994","unstructured":"[8] O'Donoughue, L.S.; Bennett, M.D. Durum wheat haploid production using maize wide-crossing, Theor. Appl. Genet., Volume 89 (1994), pp. 559-566","journal-title":"Theor. Appl. 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Wheat Genetics Symposium, Saskatoon, Saskatchewan, Canada, vol. 3, 2\u20137 August 1998, pp. 44\u201346"},{"key":"key2025102316270467202_11","first-page":"149","author":"J'aiti, F.","year":"2000","unstructured":"[11] J'aiti, F.; El Jaafari, S.; El Hadrami, I.; Nachit, M.; Baum, M.; De Buyser, J.; Picard, E. Probl\u00e9matique de la culture de microspores chez le bl\u00e9 dur Triticum turgidum L. Var. durum (Royo, C.; Nachit, M.M.; Fonza, N.Di.; Araus, J.-L., eds.), L'am\u00e9lioration du bl\u00e9 dur dans la r\u00e9gion m\u00e9diterran\u00e9enne : nouveaux d\u00e9fis, CIHEAM, Centre Udl\u2013IRTA, CIMMYT, ICARDA, 2000, pp. 149-152","journal-title":"Probl\u00e9matique de la culture de microspores chez le bl\u00e9 dur <span class=\"italique\">Triticum turgidum<\/span> L. Var. durum"},{"key":"key2025102316270467202_12","unstructured":"[12] J. De Buyser, P. Touraine, F. J'a\u00efti, R. Ha\u00efcour, E. 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Biol."},{"issue":"4","key":"key2025102316270467202_17","first-page":"483","article-title":"High production of embryoids in anther culture of pollen derived homozygous spring wheats","volume":"27","author":"Picard, E.","year":"1977","unstructured":"[17] Picard, E.; De Buyser, J. High production of embryoids in anther culture of pollen derived homozygous spring wheats, Ann. Am\u00e9lior. Plant (Paris), Volume 27 (1977) no. 4, pp. 483-488","journal-title":"Ann. Am\u00e9lior. Plant (Paris)"},{"key":"key2025102316270467202_18","first-page":"49","article-title":"Androgen\u00e8se sur des bl\u00e9s tendres en cours de s\u00e9lection. 1. L'obtention des plantes in vitro","volume":"83","author":"De Buyser, J.","year":"1979","unstructured":"[18] De Buyser, J.; Henry, Y. Androgen\u00e8se sur des bl\u00e9s tendres en cours de s\u00e9lection. 1. L'obtention des plantes in vitro, Z. Pflanzenzuecht., Volume 83 (1979), pp. 49-56","journal-title":"Z. Pflanzenzuecht."}],"container-title":["Comptes Rendus. Biologies"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/comptes-rendus.academie-sciences.fr\/biologies\/item\/10.1016\/j.crvi.2005.05.009.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T14:31:09Z","timestamp":1761229869000},"score":1,"resource":{"primary":{"URL":"https:\/\/comptes-rendus.academie-sciences.fr\/biologies\/articles\/10.1016\/j.crvi.2005.05.009\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,7,5]]},"references-count":18,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2005]]}},"alternative-id":["10.1016\/j.crvi.2005.05.009"],"URL":"https:\/\/doi.org\/10.1016\/j.crvi.2005.05.009","relation":{},"ISSN":["1631-0691","1768-3238"],"issn-type":[{"value":"1631-0691","type":"print"},{"value":"1768-3238","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,7,5]]}}}