{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:16:03Z","timestamp":1790738163923,"version":"4.1.0"},"reference-count":34,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2013,8]]},"DOI":"10.1016\/j.cryobiol.2013.05.004","type":"journal-article","created":{"date-parts":[[2013,5,28]],"date-time":"2013-05-28T01:54:32Z","timestamp":1369706072000},"page":"70-75","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":27,"title":["Effect of different cryoprotectant agents on spermatogenesis efficiency in cryopreserved and grafted neonatal mouse testicular tissue"],"prefix":"10.1016","volume":"67","author":[{"given":"Cengiz","family":"Yildiz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendan","family":"Mullen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith","family":"Jarvi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin","family":"McKerlie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirk C.","family":"Lo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cryobiol.2013.05.004_b0005","doi-asserted-by":"crossref","first-page":"2031","DOI":"10.1093\/humrep\/14.8.2031","article-title":"Pregnancies achieved after frozen\u2013thawed pronuclear oocytes obtained by intracytoplasmic sperm injection with spermatozoa extracted from frozen\u2013thawed testicular tissues from non-obstructive azoospermic men","volume":"14","author":"Al-Hasani","year":"1999","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0010","doi-asserted-by":"crossref","first-page":"1788","DOI":"10.1093\/humrep\/17.7.1788","article-title":"Retrospective multicentre study on mechanical and enzymatic preparation of fresh and cryopreserved testicular biopsies","volume":"17","author":"Baukloh","year":"2002","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0015","doi-asserted-by":"crossref","first-page":"11298","DOI":"10.1073\/pnas.91.24.11298","article-title":"Spermatogenesis following male germ-cell transplantation","volume":"22","author":"Brinster","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.cryobiol.2013.05.004_b0020","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/S0015-0282(00)01721-0","article-title":"Isolation of germ cells from human testicular tissue for low temperature storage and autotransplantation","volume":"75","author":"Brook","year":"2001","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0025","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1093\/ilar.41.4.197","article-title":"Cryopreservation of murine spermatozoa","volume":"41","author":"Critser","year":"2000","journal-title":"ILAR J."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0030","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1093\/humrep\/deh083","article-title":"Pregnancy with frozen\u2013thawed and fresh testicular biopsy after motile and immotile sperm microinjection, using the mechanical touch technique to assess viability","volume":"19","author":"De Oliveira","year":"2004","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0035","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1093\/humrep\/14.4.1034","article-title":"Diagnostic testicular biopsy and cryopreservation of testicular tissue as an alternative to repeated surgical openings in the treatment of azoospermic men","volume":"14","author":"Gianaroli","year":"1999","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0040","doi-asserted-by":"crossref","unstructured":"M. Gil-Salom, J. Romero, Y. Minguez, C. Rubio, M.J. De los Santos, J. Remoh\u00ed, A. Pellicer, Pregnancies after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa, Hum. Reprod. 11 (1996) 1309\u20131313.","DOI":"10.1093\/oxfordjournals.humrep.a019377"},{"key":"10.1016\/j.cryobiol.2013.05.004_b0045","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/j.fertnstert.2007.03.044","article-title":"Cryosurvival and spermatogenesis after allografting prepubertal mouse tissue: comparison of two cryopreservation protocols","volume":"89","author":"Goossens","year":"2008","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0050","first-page":"778","article-title":"Sperm from neonatal mammalian testes grafted in mice","volume":"15","author":"Honaramooz","year":"2000","journal-title":"Nature"},{"key":"10.1016\/j.cryobiol.2013.05.004_b0055","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1095\/biolreprod.103.018788","article-title":"Fertility and germline transmission of donor haplotype following germ cell transplantation in immunocompetent goats","volume":"69","author":"Honaramooz","year":"2003","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0060","doi-asserted-by":"crossref","first-page":"2472","DOI":"10.1093\/oxfordjournals.humrep.a019140","article-title":"Pregnancy resulting from intracytoplasmic injection of spermatozoa from a frozen\u2013thawed testicular biopsy specimen","volume":"11","author":"Hovatta","year":"1996","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0065","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1095\/biolreprod.102.004986","article-title":"Proliferation and differentiation of bovine type A spermatogonia during long-term culture","volume":"68","author":"Izadyar","year":"2003","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0070","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1093\/humrep\/del471","article-title":"Effect of cold storage and cryopreservation of immature non-human primate testicular tissue on spermatogonial stem cell potential in xenografts","volume":"22","author":"Jahnukainen","year":"2007","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0075","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1046\/j.1439-0272.2001.00459.x","article-title":"Effects of various cryopreservation media and freezing\u2013thawing on the morphology of rat testicular biopsies","volume":"33","author":"Jezek","year":"2001","journal-title":"Andrologia"},{"key":"10.1016\/j.cryobiol.2013.05.004_b0080","doi-asserted-by":"crossref","first-page":"1676","DOI":"10.1093\/humrep\/deh797","article-title":"Optimizing cryopreservation of humantesticular tissue: comparison of protocols with glycerol, propanediol and dimethylsulphoxide as cryoprotectants","volume":"20","author":"Keros","year":"2005","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0085","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.1093\/humrep\/del508","article-title":"Methods of cryopreservation of testicular tissue with viable spermatogonia in pre-pubertal boys undergoing gonadotoxic cancer treatment","volume":"22","author":"Keros","year":"2007","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0090","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1093\/humrep\/dei331","article-title":"Cryopreservation of intact testicular tissue from boys with cryptorchidism","volume":"21","author":"Kvist","year":"2006","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0095","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1016\/0011-2240(71)90035-6","article-title":"The role of cooling rates in low-temperature preservation","volume":"8","author":"Leibo","year":"1971","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2013.05.004_b0100","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1093\/humrep\/dem355","article-title":"Comparison of conditions for cryopreservation of testicular tissue from immature mice","volume":"23","author":"Milazzo","year":"2008","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0105","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1095\/biolreprod64.5.1409","article-title":"Primate spermatogonial stem cells colonize mouse testes","volume":"64","author":"Nagano","year":"2001","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0110","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1016\/S0015-0282(02)04345-5","article-title":"Long-term survival of human spermatogonial stem cells in mouse testes","volume":"78","author":"Nagano","year":"2002","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0115","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1093\/humupd\/3.3.267","article-title":"Transplantation in reproductive medicine: previous experience, present knowledge and future prospects","volume":"3","author":"Nugent","year":"1997","journal-title":"Hum. Reprod. Update"},{"key":"10.1016\/j.cryobiol.2013.05.004_b0120","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1093\/humrep\/12.4.734","article-title":"Fertilization and pregnancy using intentionally cryopreserved testicular tissue as the sperm source for intracytoplasmic sperm injection in 10 men with non-obstructive azoospermia","volume":"12","author":"Oates","year":"1997","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0125","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.1093\/oxfordjournals.humrep.a019376","article-title":"Pregnancy resulting from intracytoplasmic injection of cryopreserved spermatozoa recovered from testicular biopsy","volume":"11","author":"Podsiadly","year":"1996","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0130","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/S0015-0282(16)48918-1","article-title":"Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid","volume":"44","author":"Quinn","year":"1985","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0135","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1080\/1464727992000198531","article-title":"Germ cell transplantation in the male: animal studies with a human perspective","volume":"2","author":"Schlatt","year":"1999","journal-title":"Hum. Fertil. (Camb.)"},{"key":"10.1016\/j.cryobiol.2013.05.004_b0140","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1530\/rep.0.1240339","article-title":"Spermatogenesis and steroidogenesis in mouse, hamster and monkey testicular tissue after cryopreservation and heterotopic grafting to castrated hosts","volume":"124","author":"Schlatt","year":"2002","journal-title":"Reproduction"},{"key":"10.1016\/j.cryobiol.2013.05.004_b0145","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/S0015-0282(99)00359-3","article-title":"Extraction of testicular sperm from previously cryopreserved tissue in couples with or without transport of oocytes and testicular tissue","volume":"72","author":"Scholtes","year":"1999","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0150","doi-asserted-by":"crossref","first-page":"3039","DOI":"10.1093\/humrep\/17.12.3039","article-title":"Birth of offspring following transplantation of cryopreserved immature testicular pieces and in-vitro microinsemination","volume":"17","author":"Shinohara","year":"2002","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0155","doi-asserted-by":"crossref","first-page":"1064","DOI":"10.1095\/biolreprod.102.009977","article-title":"Restoration of spermatogenesis in infertile mice by Sertoli cell transplantation","volume":"68","author":"Shinohara","year":"2003","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0160","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1007\/s003359901145","article-title":"Large numbers of mice established by in vitro fertilization with cryopreserved spermatozoa: implications and applications for genetic resource banks, mutagenesis screens, and mouse backcrosses","volume":"10","author":"Thornton","year":"1999","journal-title":"Mamm. Genome"},{"key":"10.1016\/j.cryobiol.2013.05.004_b0165","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1093\/humrep\/14.5.1274","article-title":"Testicular biopsy gun needle biopsy in collecting spermatozoa for intracytoplasmic injection, cryopreservation and histology","volume":"14","author":"Tuuri","year":"1999","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2013.05.004_b0170","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1046\/j.0303-4569.2003.00591.x","article-title":"Morphological and morphometric attributes of epididymal and testicular spermatozoa following surgical sperm retrieval for obstructive and nonobstructive azoospermia","volume":"35","author":"Wood","year":"2003","journal-title":"Andrologia"}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224013001569?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224013001569?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T19:20:54Z","timestamp":1539631254000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224013001569"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["S0011224013001569"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2013.05.004","relation":{},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2013,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Effect of different cryoprotectant agents on spermatogenesis efficiency in cryopreserved and grafted neonatal mouse testicular tissue","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2013.05.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}