{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T14:30:54Z","timestamp":1785767454316,"version":"3.56.0"},"reference-count":37,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","award":["2009\/52760-3"],"award-info":[{"award-number":["2009\/52760-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2016,4]]},"DOI":"10.1016\/j.cryobiol.2016.02.001","type":"journal-article","created":{"date-parts":[[2016,2,14]],"date-time":"2016-02-14T06:58:09Z","timestamp":1455433089000},"page":"135-140","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":37,"title":["Effect of reduced glutathione (GSH) in canine sperm cryopreservation: In\u00a0vitro and in\u00a0vivo evaluation"],"prefix":"10.1016","volume":"72","author":[{"given":"C.F.","family":"Lucio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.C.G.","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.M.","family":"Regazzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.S.R.","family":"Angrimani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Nichi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.E.O.","family":"Assump\u00e7\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.I.","family":"Vannucchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cryobiol.2016.02.001_bib1","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1093\/molehr\/3.3.169","article-title":"Molecular mechanisms regulating human sperm function","volume":"3","author":"Aitken","year":"1997","journal-title":"Mol. Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib2","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1097\/00001703-199404000-00004","article-title":"Pathophysiology of Human Spermatozoa","volume":"6","author":"Aitken","year":"1994","journal-title":"Curr. Opin. Obstet. Gyn"},{"key":"10.1016\/j.cryobiol.2016.02.001_bib3","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1530\/jrf.0.0970441","article-title":"Use of a xanthine-oxidase free-radical generating-system to investigate the cytotoxic effects of reactive oxygen species on human spermatozoa","volume":"97","author":"Aitken","year":"1993","journal-title":"J.\u00a0Reprod. Fertil."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib4","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1002\/mrd.1120230108","article-title":"Role of glutathione-peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid-peroxidation","volume":"23","author":"Alvarez","year":"1989","journal-title":"Gamete Res."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib5","article-title":"Impacts of oxidative stress and antioxidants on semen functions","volume":"2010","author":"Bansal","year":"2010","journal-title":"Veterinary Med. Int."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib6","doi-asserted-by":"crossref","first-page":"1716","DOI":"10.1016\/j.fertnstert.2007.09.007","article-title":"Sperm nuclear DNA fragmentation and mitochondrial activity in men with varicocele","volume":"90","author":"Blumer","year":"2008","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib7","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1111\/j.1464-410X.2011.10240.x","article-title":"Effect of varicocele on sperm function and semen oxidative stress","volume":"109","author":"Blumer","year":"2012","journal-title":"BJU Int."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib8","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.cryobiol.2010.09.001","article-title":"Effects of antioxidants on post-thawed bovine sperm and oxidative stress parameters: antioxidants protect DNA integrity against cryodamage","volume":"61","author":"Bucak","year":"2010","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2016.02.001_bib9","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/S0076-6879(78)52032-6","article-title":"Microsomal lipid peroxidation","volume":"52","author":"Buege","year":"1978","journal-title":"Methods Enzym."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib10","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1111\/j.1439-0531.2006.00810.x","article-title":"Practical techniques for bovine sperm simultaneous fluorimetric assessment of plasma, acrosomal and mitochondrial membranes","volume":"42","author":"Celeghini","year":"2007","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib11","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1023\/A:1009844109023","article-title":"Sperm chromatin structure assay is useful for fertility assessment","volume":"22","author":"Evenson","year":"2000","journal-title":"Methods Cell Sci. An Official J. Soc. Vitro Biol."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib12","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1111\/j.1439-0531.2009.01418.x","article-title":"Cryopreservation of Canine Semen - New Challenges","volume":"44","author":"Farstad","year":"2009","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib13","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1111\/j.1365-2605.2007.00756.x","article-title":"Supplementation of the dilution medium after thawing with reduced glutathione improves function and the in\u00a0vitro fertilizing ability of frozen-thawed bull spermatozoa","volume":"31","author":"Gadea","year":"2008","journal-title":"Int. J. Androl."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib14","doi-asserted-by":"crossref","first-page":"749","DOI":"10.2164\/jandrol.05057","article-title":"Supplementation of the thawing media with reduced glutathione improves function and the in\u00a0vitro fertilizing ability of boar spermatozoa after cryopreservation","volume":"26","author":"Gadea","year":"2005","journal-title":"J.\u00a0Androl."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib15","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.cryobiol.2010.12.001","article-title":"Reduced glutathione content in human sperm is decreased after cryopreservation: effect of the addition of reduced glutathione to the freezing and thawing extenders","volume":"62","author":"Gadea","year":"2011","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2016.02.001_bib16","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1016\/j.theriogenology.2003.11.013","article-title":"Decrease in glutathione content in boar sperm after cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders","volume":"62","author":"Gadea","year":"2004","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2016.02.001_bib17","doi-asserted-by":"crossref","DOI":"10.1071\/RDv23n1Ab75","article-title":"Influence of cryopreservation on the susceptibility of goat sperm against different reactive oxygen species","volume":"23","author":"Goes","year":"2011","journal-title":"Reprod. Fert. Dev."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib18","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1046\/j.1365-2605.1997.00049.x","article-title":"Influence of oxygen tension on reactive oxygen species production and human sperm function","volume":"20","author":"Griveau","year":"1997","journal-title":"Int. J. Androl."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib19","doi-asserted-by":"crossref","first-page":"141","DOI":"10.3109\/10715769309111598","article-title":"Free radicals in disease processes: a compilation of cause and consequence","volume":"19","author":"Gutteridge","year":"1993","journal-title":"Free Radic. Res. Commun."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib20","series-title":"Free Radicals in Biology and Medicine","author":"Halliwell","year":"1991"},{"key":"10.1016\/j.cryobiol.2016.02.001_bib21","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1111\/j.1365-2605.1987.tb00385.x","article-title":"Cytochemical and ultracytochemical demonstration of cytochrome c oxidase in spermatozoa and dynamics of its changes accompanying ageing or induced by stress","volume":"10","author":"Hrudka","year":"1987","journal-title":"Int. J. Androl."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib22","series-title":"Canine and Feline Theriogenology","author":"Johnston","year":"2001"},{"key":"10.1016\/j.cryobiol.2016.02.001_bib23","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.mam.2008.05.005","article-title":"Regulation of glutathione synthesis","volume":"30","author":"Lu","year":"2009","journal-title":"Mol. Asp. Med."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib24","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1111\/j.1439-0531.2012.02152.x","article-title":"Assessment of different functional parameters of frozen-thawed buffalo spermatozoa by using cytofluorimetric determinations","volume":"48","author":"Minervini","year":"2013","journal-title":"Reprod. Domest. Anim."},{"issue":"Suppl 2","key":"10.1016\/j.cryobiol.2016.02.001_bib25","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1111\/j.1439-0531.2009.01434.x","article-title":"Influence of ascorbic acid and glutathione antioxidants on frozen-thawed canine semen","volume":"44","author":"Monteiro","year":"2009","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib26","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.theriogenology.2006.08.002","article-title":"Roles of lipid peroxidation and cytoplasmic droplets on in\u00a0vitro fertilization capacity of sperm collected from bovine epididymides stored at 4 and 34 degrees C","volume":"67","author":"Nichi","year":"2007","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2016.02.001_bib27","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1016\/S0891-5849(01)00724-9","article-title":"Reactive oxygen species, antioxidants, and the mammalian thioredoxin system","volume":"31","author":"Nordberg","year":"2001","journal-title":"Free Radic. Bio Med."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib28","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0003-2697(79)90738-3","article-title":"Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction","volume":"95","author":"Ohkawa","year":"1979","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib29","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1111\/and.12079","article-title":"Semen analysis of Golden Retriever healthy dogs and those affected by muscular dystrophy","volume":"46","author":"Peres","year":"2014","journal-title":"Andrologia"},{"key":"10.1016\/j.cryobiol.2016.02.001_bib30","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1002\/mrd.20686","article-title":"Impact of cryopreservation and reactive oxygen species on DNA integrity, lipid peroxidation, and functional parameters in ram sperm","volume":"74","author":"Peris","year":"2007","journal-title":"Mol. Reproduction Dev."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib31","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1111\/j.1439-0531.2010.01719.x","article-title":"Effect of pre-freeze addition of cysteine hydrochloride and reduced glutathione in semen of crossbred jersey bulls on sperm parameters and conception rates","volume":"46","author":"Perumal","year":"2011","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib32","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1016\/S0093-691X(03)00171-7","article-title":"Effect of technical settings on canine semen motility parameters measured by the Hamilton-Thorne analyzer","volume":"60","author":"Rijsselaere","year":"2003","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2016.02.001_bib33","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1016\/S0090-4295(96)00313-5","article-title":"Role of reactive oxygen species in male infertility","volume":"48","author":"Sharma","year":"1996","journal-title":"Urology"},{"key":"10.1016\/j.cryobiol.2016.02.001_bib34","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1136\/vr.138.7.154","article-title":"Comparisons of intravaginal and intrauterine insemination of bitches with fresh or frozen semen","volume":"138","author":"Silva","year":"1996","journal-title":"Veterinary Rec."},{"key":"10.1016\/j.cryobiol.2016.02.001_bib35","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1530\/REP-13-0123","article-title":"Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in\u00a0vitro development outcome","volume":"146","author":"Simoes","year":"2013","journal-title":"Reproduction"},{"key":"10.1016\/j.cryobiol.2016.02.001_bib36","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1016\/j.theriogenology.2007.01.009","article-title":"Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: comparison between two extenders","volume":"67","author":"Stradaioli","year":"2007","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2016.02.001_bib37","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0093-691X(01)00664-1","article-title":"Computer assisted semen analyzers in andrology research and veterinary practice","volume":"57","author":"Verstegen","year":"2002","journal-title":"Theriogenology"}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224016300050?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224016300050?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T10:29:08Z","timestamp":1718360948000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224016300050"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,4]]}},"alternative-id":["S0011224016300050"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2016.02.001","relation":{},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2016,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Effect of reduced glutathione (GSH) in canine sperm cryopreservation: In\u00a0vitro and in\u00a0vivo evaluation","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2016.02.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2016 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}