{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:12:05Z","timestamp":1791994325863,"version":"4.4.0"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Natural Sciences and Engineering Research Council (NSERC)","award":["171189"],"award-info":[{"award-number":["171189"]}]},{"name":"Programme de soutien \u00e0 l'innovation en agroalimentaire"},{"DOI":"10.13039\/501100000040","name":"Agriculture and Agri-Food Canada","doi-asserted-by":"publisher","award":["810264"],"award-info":[{"award-number":["810264"]}],"id":[{"id":"10.13039\/501100000040","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Centre de recherche en science animales de Deschambault"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2017,2]]},"DOI":"10.1016\/j.cryobiol.2016.12.010","type":"journal-article","created":{"date-parts":[[2016,12,29]],"date-time":"2016-12-29T21:15:13Z","timestamp":1483046113000},"page":"19-24","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Novel technical strategies to optimize cryopreservation of goat semen using cholesterol-loaded cyclodextrin"],"prefix":"10.1016","volume":"74","author":[{"given":"Vianney M.","family":"Salmon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Leclerc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janice L.","family":"Bailey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cryobiol.2016.12.010_bib1","first-page":"318","article-title":"Toxic effect of lysolecithin on sperm. A preliminary report","volume":"17","author":"Aamdal","year":"1965","journal-title":"Nord. Vet. Med."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib2","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1530\/jrf.0.0680275","article-title":"Factors in seminal plasma of bulls that affect the viability and motility of spermatozoa","volume":"68","author":"Baas","year":"1983","journal-title":"J.\u00a0reproduction Fertil."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib3","doi-asserted-by":"crossref","first-page":"690","DOI":"10.2527\/2004.823690x","article-title":"Pregnancy rates of mares inseminated with semen cooled for 18 hours and then frozen","volume":"82","author":"Backman","year":"2004","journal-title":"J.\u00a0Anim. Sci."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib4","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1095\/biolreprod63.5.1531","article-title":"Seminal plasma proteins revert the cold-shock damage on ram sperm membrane","volume":"63","author":"Barrios","year":"2000","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib5","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1095\/biolreprod.103.022996","article-title":"Low-density lipoprotein fraction from hen's egg yolk decreases the binding of the major proteins of bovine seminal plasma to sperm and prevents lipid efflux from the sperm membrane","volume":"70","author":"Bergeron","year":"2004","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib6","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1002\/mrd.20310","article-title":"Isolation and characterization of the major proteins of ram seminal plasma","volume":"71","author":"Bergeron","year":"2005","journal-title":"Mol. Reprod. Dev."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib7","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/0021-9673(95)00011-B","article-title":"Effect of glycosylation on the heparin-binding capability of boar and stallion seminal plasma proteins","volume":"711","author":"Calvete","year":"1995","journal-title":"J.\u00a0Chromatogr. A"},{"key":"10.1016\/j.cryobiol.2016.12.010_bib8","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1002\/mrd.1120090306","article-title":"Induction of the acrosome reaction in the spermatozoa of the goat by secretions of the male accessory glands and milk","volume":"9","author":"Courtens","year":"1984","journal-title":"Gamete Res."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib9","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1006\/cryo.1998.2137","article-title":"Are lipid phase transitions responsible for chilling damage in human platelets?","volume":"38","author":"Crowe","year":"1999","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2016.12.010_bib10","doi-asserted-by":"crossref","first-page":"7560","DOI":"10.1073\/pnas.78.12.7560","article-title":"Timing of fertilization in mammals: sperm cholesterol\/phospholipid ratio as a determinant of the capacitation interval","volume":"78","author":"Davis","year":"1981","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib11","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1051\/rnd:19950507","article-title":"Effect of short photoperiodic cycles on male genital tract and testicular parameters in male goats (Capra hircus)","volume":"35","author":"Delgadillo","year":"1995","journal-title":"Reprod. Nutr. Dev."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib12","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1530\/jrf.0.0380147","article-title":"The effects of bovine seminal plasma on the impedance change frequency and glycolysis of bovine epididymal spermatozoa","volume":"38","author":"Dott","year":"1974","journal-title":"J.\u00a0reproduction Fertil."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib13","doi-asserted-by":"crossref","first-page":"3385","DOI":"10.1093\/humrep\/dei236","article-title":"The effect of chilling on membrane lipid phase transition in human oocytes and zygotes","volume":"20","author":"Ghetler","year":"2005","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib14","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0093-691X(00)00328-9","article-title":"Influence of centrifugation and different extenders on post-thaw sperm quality of ram semen","volume":"54","author":"Gil","year":"2000","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2016.12.010_bib15","first-page":"747","article-title":"The effects of ex\u00a0vivo cold-storage on cryopreservation of the goat (Caprus hircus) epididymal sperm","volume":"11","author":"Hoseinzadeh-Sani","year":"2013","journal-title":"Iran. J. Reprod. Med."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib16","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1262\/jrd1955.8.113","article-title":"Studies on the egg yolk coagulation enzyme in goat semen: IV. On the position of yolk constituents attacked by the coagulating enzyme","volume":"8","author":"Iritani","year":"1963","journal-title":"Jpn. J. Anim. Reprod."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib17","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.cryobiol.2013.06.001","article-title":"Optimizing conditions for treating goat semen with cholesterol-loaded cyclodextrins prior to freezing to improve cryosurvival","volume":"67","author":"Konyali","year":"2013","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2016.12.010_bib18","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S0378-4320(00)00156-1","article-title":"Production and storage of goat semen for artificial insemination","volume":"62","author":"Leboeuf","year":"2000","journal-title":"Anim. Reprod. Sci."},{"issue":"Suppl 2","key":"10.1016\/j.cryobiol.2016.12.010_bib19","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1111\/j.1439-0531.2008.01188.x","article-title":"Management of goat reproduction and insemination for genetic improvement in France","volume":"43","author":"Leboeuf","year":"2008","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib20","series-title":"SAS System for Mixed Models","author":"Littell","year":"1996"},{"key":"10.1016\/j.cryobiol.2016.12.010_bib21","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0165-0378(01)00098-5","article-title":"Role of seminal plasma phospholipid-binding proteins in sperm membrane lipid modification that occurs during capacitation","volume":"53","author":"Manjunath","year":"2002","journal-title":"J.\u00a0Reprod. Immunol."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib22","first-page":"217","article-title":"Seminal plasma proteins: functions and interaction with protective agents during semen preservation","volume":"65","author":"Manjunath","year":"2007","journal-title":"Soc. Reprod. Fertil. Suppl."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib23","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1002\/mrd.10369","article-title":"Novel purification method for mammalian seminal plasma phospholipid-binding proteins reveals the presence of a novel member of this family of protein in stallion seminal fluid","volume":"66","author":"Menard","year":"2003","journal-title":"Mol. Reprod. Dev."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib24","doi-asserted-by":"crossref","first-page":"826","DOI":"10.2527\/2006.844826x","article-title":"Cholesterol-loaded cyclodextrins added to fresh bull ejaculates improve sperm cryosurvival","volume":"84","author":"Moce","year":"2006","journal-title":"J.\u00a0Anim. Sci."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib25","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.anireprosci.2009.06.013","article-title":"Treating ram sperm with cholesterol-loaded cyclodextrins improves cryosurvival","volume":"118","author":"Moce","year":"2010","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib26","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.cryobiol.2005.07.004","article-title":"Adding cholesterol to the stallion sperm plasma membrane improves cryosurvival","volume":"51","author":"Moore","year":"2005","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2016.12.010_bib27","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1111\/rda.12225","article-title":"Effect of different levels of glycerol and cholesterol-loaded cyclodextrin on cryosurvival of ram spermatozoa","volume":"49","author":"Motamedi-Mojdehi","year":"2014","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib28","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.1095\/biolreprod.102.011445","article-title":"A\u00a0triple-stain flow cytometric method to assess plasma- and acrosome-membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles","volume":"68","author":"Nagy","year":"2003","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib29","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.anireprosci.2009.08.011","article-title":"Cholesterol addition protects membrane intactness during cryopreservation of stallion sperm","volume":"118","author":"Oliveira","year":"2010","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib30","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1530\/jrf.0.1120095","article-title":"Deterioration of goat spermatozoa in skimmed milk-based extenders as a result of oleic acid released by the bulbourethral lipase BUSgp60","volume":"112","author":"Pellicer-Rubio","year":"1998","journal-title":"J.\u00a0reproduction Fertil."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib31","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1038\/164666a0","article-title":"Revival of spermatozoa after vitrification and dehydration at low temperatures","volume":"164","author":"Polge","year":"1949","journal-title":"Nature"},{"key":"10.1016\/j.cryobiol.2016.12.010_bib32","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.anireprosci.2005.07.002","article-title":"The post-thaw quality of ram sperm held for 0 to 48 h at 5 degrees C prior to cryopreservation","volume":"93","author":"Purdy","year":"2006","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib33","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.cryobiol.2003.12.001","article-title":"Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm","volume":"48","author":"Purdy","year":"2004","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2016.12.010_bib34","doi-asserted-by":"crossref","first-page":"528","DOI":"10.2527\/jas1973.372528x","article-title":"Effect of dilution, seminal plasma and incubation period on cold shock susceptibility of boar spermatozoa","volume":"37","author":"Pursel","year":"1973","journal-title":"J.\u00a0Anim. Sci."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib35","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1071\/BI9820305","article-title":"Effects of seminal plasma and of its removal and of egg yolk in the diluent on the survival of fresh and frozen-thawed spermatozoa of the Angora goat","volume":"35","author":"Ritar","year":"1982","journal-title":"Aust. J. Biol. Sci."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib36","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1002\/j.1939-4640.2004.tb02806.x","article-title":"Survival and fertility of boar spermatozoa after freeze-thawing in extender supplemented with butylated hydroxytoluene","volume":"25","author":"Roca","year":"2004","journal-title":"J.\u00a0Androl."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib37","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1002\/j.1939-4640.2005.tb02867.x","article-title":"Survival and in\u00a0vitro fertility of boar spermatozoa frozen in the presence of superoxide dismutase and\/or catalase","volume":"26","author":"Roca","year":"2005","journal-title":"J.\u00a0Androl."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib38","first-page":"205","article-title":"The Canadian Council on Animal Care\u2013its guidelines and policy directives: the veterinarian's responsibility","volume":"55","author":"Rowsell","year":"1991","journal-title":"Can. J. Vet. Res."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib39","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1038\/179318b0","article-title":"Egg yolk-coagulating enzyme in the semen and Cowper's gland of the goat","volume":"179","author":"Roy","year":"1957","journal-title":"Nature"},{"key":"10.1016\/j.cryobiol.2016.12.010_bib40","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1095\/biolreprod.115.128553","article-title":"Cholesterol-loaded cyclodextrin increases the cholesterol content of goat sperm to improve cold and osmotic resistance and maintain sperm function after cryopreservation","volume":"94","author":"Salmon","year":"2016","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib41","first-page":"272","article-title":"Effect of different extenders and washing of seminal plasma on buck semen storage at 5\u00b0C","volume":"6","author":"Salvador","year":"2007","journal-title":"J.\u00a0Animal Veterinary Adv."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib42","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1002\/j.1939-4640.2004.tb02783.x","article-title":"Interaction of PDC-109, the major secretory protein from bull seminal vesicles, with bovine sperm membrane Ca2+-ATPase","volume":"25","author":"Sanchez-Luengo","year":"2004","journal-title":"J.\u00a0Androl."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib43","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1002\/mrd.1080350107","article-title":"Isolation and biochemical characterization of heparin-binding proteins from boar seminal plasma: a dual role for spermadhesins in fertilization","volume":"35","author":"Sanz","year":"1993","journal-title":"Mol. Reprod. Dev."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib44","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.bbalip.2004.09.008","article-title":"Cloning and seasonal secretion of the pancreatic lipase-related protein 2 present in goat seminal plasma","volume":"1686","author":"Sias","year":"2005","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.cryobiol.2016.12.010_bib45","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1136\/vr.135.7.160","article-title":"Cold resistance of live boar spermatozoa during incubation after ejaculation","volume":"135","author":"Tamuli","year":"1994","journal-title":"Vet. Rec."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib46","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1111\/rda.12291","article-title":"Addition of cholesterol-loaded cyclodextrins to the thawing extender: effects on boar sperm quality","volume":"49","author":"Tomas","year":"2014","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2016.12.010_bib47","doi-asserted-by":"crossref","first-page":"3235","DOI":"10.1074\/jbc.274.5.3235","article-title":"Cholesterol efflux-mediated signal transduction in mammalian sperm. beta-cyclodextrins initiate transmembrane signaling leading to an increase in protein tyrosine phosphorylation and capacitation","volume":"274","author":"Visconti","year":"1999","journal-title":"J.\u00a0Biol. Chem."}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224016301328?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224016301328?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T13:54:52Z","timestamp":1718978092000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224016301328"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2]]},"references-count":47,"alternative-id":["S0011224016301328"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2016.12.010","relation":{},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2017,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Novel technical strategies to optimize cryopreservation of goat semen using cholesterol-loaded cyclodextrin","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2016.12.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}