{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:53:29Z","timestamp":1790834009859,"version":"4.1.0"},"reference-count":71,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2018,1,2]],"date-time":"2018-01-02T00:00:00Z","timestamp":1514851200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100001250","name":"Morris Animal Foundation","doi-asserted-by":"publisher","award":["D15ZO-053"],"award-info":[{"award-number":["D15ZO-053"]}],"id":[{"id":"10.13039\/100001250","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001792","name":"James Cook University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001792","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2018,2]]},"DOI":"10.1016\/j.cryobiol.2017.12.095","type":"journal-article","created":{"date-parts":[[2017,12,26]],"date-time":"2017-12-26T20:29:42Z","timestamp":1514320182000},"page":"18-25","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":27,"special_numbering":"C","title":["A two-step dilution tris-egg yolk extender containing Equex STM significantly improves sperm cryopreservation in the African wild dog (Lycaon pictus)"],"prefix":"10.1016","volume":"80","author":[{"given":"Femke","family":"Van den Berghe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique Christina Johanna","family":"Paris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael Brent","family":"Briggs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenche Kristin","family":"Farstad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damien Boyd Bertrand Paul","family":"Paris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cryobiol.2017.12.095_bib1","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1095\/biolreprod2.Supplement_2.128","article-title":"Sperm capacitation and fertilization in mammals","volume":"2","author":"Bedford","year":"1970","journal-title":"Biol. Reprod. Suppl."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib2","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.anireprosci.2010.01.009","article-title":"Freezing canine sperm: comparison of semen extenders containing Equex and LDL (Low Density Lipoproteins)","volume":"119","author":"Bencharif","year":"2010","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib3","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1016\/j.theriogenology.2011.05.003","article-title":"Effect of semen collection method on sperm motility of gray wolves (Canis lupus) and domestic dogs (C. l. familiaris)","volume":"76","author":"Christensen","year":"2011","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib4","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1016\/j.theriogenology.2012.04.008","article-title":"Comparative cryobiological traits and requirements for gametes and gonadal tissues collected from wildlife species","volume":"78","author":"Comizzoli","year":"2012","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib5","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1530\/ror.0.0050046","article-title":"Cryopreservation of semen from domestic livestock","volume":"5","author":"Curry","year":"2000","journal-title":"Rev. Reprod."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib6","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.theriogenology.2008.09.004","article-title":"The importance and potential of artificial insemination in CANDES (companion animals, non-domestic, endangered species)","volume":"71","author":"Durrant","year":"2009","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib7","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1111\/j.1439-0531.2009.01418.x","article-title":"Cryopreservation of canine semen - new challenges","volume":"44","author":"Farstad","year":"2009","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib8","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1371\/journal.pone.0090292","article-title":"Generalized drivers in the mammalian endangerment process","volume":"9","author":"Gonzalez-Suarez","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib9","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/S0378-4320(98)00119-5","article-title":"Characteristics of fresh and frozen\u2013thawed red wolf (Canis rufus) spermatozoa","volume":"53","author":"Goodrowe","year":"1998","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib10","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/0011-2240(92)90004-L","article-title":"Cryopreservation of poultry sperm: the enigma of glycerol","volume":"29","author":"Hammerstedt","year":"1992","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib11","first-page":"315","article-title":"Establishment of assisted reproduction technologies in female and male African wild dogs (Lycaon pictus)","volume":"57","author":"Hermes","year":"2001","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib12","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0378-4320(00)00152-4","article-title":"Basic aspects of frozen storage of semen","volume":"62","author":"Holt","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib13","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0093-691X(99)00239-3","article-title":"Fundamental aspects of sperm cryobiology: the importance of species and individual differences","volume":"53","author":"Holt","year":"2000","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib14","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1111\/rda.12836","article-title":"Research on reproduction is essential for captive breeding of endangered carnivore species","volume":"52","author":"Jewgenow","year":"2017","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib15","series-title":"Semen Collection, Evaluation and Preservation, Canine and Feline Theriogenology","first-page":"287","author":"Johnston","year":"2001"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib16","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.anireprosci.2006.08.017","article-title":"Studies of male reproduction in captive African wild dogs (Lycaon pictus)","volume":"100","author":"Johnston","year":"2007","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib17","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.theriogenology.2009.09.011","article-title":"Effects of cryopreservation on phosphatidylserine translocation, intracellular hydrogen peroxide, and DNA integrity in canine sperm","volume":"73","author":"Kim","year":"2010","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib18","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1016\/j.theriogenology.2009.07.015","article-title":"The influence of cryopreservation and seminal plasma on the chromatin structure of dog spermatozoa","volume":"72","author":"Koderle","year":"2009","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib19","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/S0093-691X(05)80057-3","article-title":"The limitation of conventional breeding programs and the need and promise of assisted reproduction in nondomestic species","volume":"41","author":"Lasley","year":"1994","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib20","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1530\/REP-11-0310","article-title":"Sperm surface changes and physiological consequences induced by sperm handling and storage","volume":"142","author":"Leahy","year":"2011","journal-title":"Reproduction"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib21","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s00441-005-1097-5","article-title":"DNA damage to spermatozoa has impacts on fertilization and pregnancy","volume":"322","author":"Lewis","year":"2005","journal-title":"Cell Tissue Res."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib22","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/S0093-691X(99)00106-5","article-title":"Comparison of fertility data from vaginal vs intrauterine insemination of frozen-thawed dog semen: a retrospective study","volume":"52","author":"Linde-Forsberg","year":"1999","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib23","doi-asserted-by":"crossref","first-page":"5323","DOI":"10.1073\/pnas.1602893113","article-title":"Anthropogenic disturbances jeopardize biodiversity conservation within tropical rainforest reserves","volume":"113","author":"Martinez-Ramos","year":"2016","journal-title":"Proc. Natl. Acad. Sci. Unit. States Am."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib24","doi-asserted-by":"crossref","first-page":"2047","DOI":"10.1016\/j.theriogenology.2006.06.004","article-title":"Comparing ethylene glycol with glycerol for cryopreservation of canine semen in egg-yolk TRIS extenders","volume":"66","author":"Martins-Bessa","year":"2006","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib25","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1016\/j.theriogenology.2014.06.014","article-title":"Comparison of endoscopic-assisted transcervical and laparotomy insemination with frozen-thawed dog semen: a retrospective clinical study","volume":"82","author":"Mason","year":"2014","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib26","series-title":"Embryo Transfer Newsletter","first-page":"10","article-title":"Consensus discussion on artificial insemination in companion animals, non-domestic, and endangered species (CANDES)","author":"Mastromonaco","year":"2011"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib27","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1038\/s41598-017-00521-0","article-title":"Interspecific differences in how habitat degradation affects escape response","volume":"7","author":"McCormick","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib28","doi-asserted-by":"crossref","first-page":"1898","DOI":"10.1016\/j.theriogenology.2005.03.026","article-title":"Influence of extender, freezing rate, and thawing rate on post-thaw motility, viability and morphology of coyote (Canis latrans) spermatozoa","volume":"64","author":"Minter","year":"2005","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib29","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.ygcen.2012.05.008","article-title":"Seasonal changes in steroid hormone profiles, body weight, semen quality, and the reproductive tract in captive African wild dogs (Lycaon pictus) in South Africa","volume":"178","author":"Newell-Fugate","year":"2012","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib30","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.theriogenology.2006.01.001","article-title":"Intravaginal insemination of bitches with fresh and frozen-thawed semen with addition of prostatic fluid: use of an infusion pipette and the Osiris catheter","volume":"66","author":"Ni\u017ca\u0144ski","year":"2006","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib31","first-page":"365","article-title":"Effects of three cryopreservation methods and two semen extenders on the quality of dog semen after thawing","volume":"57","author":"Nizanski","year":"2001","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib32","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0378-4320(86)90054-0","article-title":"Changes in acrosome morphology during cooling and freezing of dog semen","volume":"12","author":"Oettl\u00e9","year":"1986","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib33","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1016\/S0022-5347(17)32650-2","article-title":"Electroejaculation does not impair sperm motility in the beagle dog: a comparative study of electroejaculation and collection by artificial vagina","volume":"152","author":"Ohl","year":"1994","journal-title":"J. Urol."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib34","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/0093-691X(89)90550-5","article-title":"Influence of extender, cryoperservative and seminal processing procedures on postthaw motility of canine spermatozoa frozen in straws","volume":"31","author":"Olar","year":"1989","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib35","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1095\/biolreprod19.1.101","article-title":"Sperm transport in the reproductive tract of the female rabbit: I. The rapid transit phase of transport","volume":"19","author":"Overstreet","year":"1978","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib36","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1095\/biolreprod19.1.115","article-title":"Sperm transport in the reproductive tract of the female rabbit: II. The sustained phase of transport","volume":"19","author":"Overstreet","year":"1978","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib37","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1530\/rep.1.00538","article-title":"Changes in semen quality and morphology of the reproductive tract of the male tammar wallaby parallel seasonal breeding activity in the female","volume":"130","author":"Paris","year":"2005","journal-title":"Reproduction"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib38","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.theriogenology.2008.09.018","article-title":"Proceedings of the IETS 2009 post-conference symposium: implementation of artificial insemination in CANDES","volume":"71","author":"Paris","year":"2009","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib39","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1530\/REP-08-0036","article-title":"A single, mild, transient scrotal heat stress causes DNA damage, subfertility and impairs formation of blastocysts in mice","volume":"136","author":"Paul","year":"2008","journal-title":"Reproduction"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib40","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1016\/S0093-691X(00)00397-6","article-title":"Effects of Equex, one- or two-step dilution, and two freezing and thawing rates on post-thaw survival of dog spermatozoa","volume":"54","author":"Pe\u00f1a","year":"2000","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib41","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/S0093-691X(00)00384-8","article-title":"Effects of spermatozoal concentration and post-thaw dilution rate on survival after thawing of dog spermatozoa","volume":"54","author":"Pena","year":"2000","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib42","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0093-691X(98)00122-8","article-title":"Effect of different glycerol treatments on frozen-thawed dog sperm longevity and acrosomal integrity","volume":"50","author":"Pe\u00f1a","year":"1998","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib43","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1111\/j.1439-0531.1998.tb01377.x","article-title":"Effects of sodium dodecyl sulphate on post-thaw dog semen quality during in vitro incubation at 39\u00b0C and 22\u00b0C","volume":"33","author":"Pe\u00f1a","year":"1998","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib44","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1046\/j.1439-0531.2003.00391.x","article-title":"Studies on the intracellular Ca2+ concentration of frozen\u2013thawed dog spermatozoa: influence of equex from different sources, two thawing diluents and post-thaw incubation in capacitating conditions","volume":"38","author":"Pe\u00f1a","year":"2003","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib45","doi-asserted-by":"crossref","first-page":"1975","DOI":"10.1071\/AN16079","article-title":"Revisiting summer infertility in the pig: could heat stress-induced sperm DNA damage negatively affect early embryo development?","volume":"57","author":"Pe\u00f1a","year":"2016","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib46","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.1016\/j.theriogenology.2004.02.014","article-title":"Influence of glucose and fructose in the extender during long-term storage of chilled canine semen","volume":"62","author":"Ponglowhapan","year":"2004","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib47","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/rda.12299","article-title":"Current knowledge on the transport and fate of spermatozoa in the reproductive tract of the bitch","volume":"49","author":"Rijsselaere","year":"2014","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib48","doi-asserted-by":"crossref","first-page":"248","DOI":"10.21825\/vdt.87276","article-title":"Preservation and shipment of chilled and cryopreserved dog semen","volume":"80","author":"Rijsselaere","year":"2011","journal-title":"Vlaams Diergeneeskundig Tijdschrift"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib49","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1016\/j.theriogenology.2003.09.008","article-title":"Effect of blood admixture on in vitro survival of chilled and frozen-thawed canine spermatozoa","volume":"61","author":"Rijsselaere","year":"2004","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib50","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1111\/j.1439-0531.1998.tb01370.x","article-title":"Cryosurvival of dog spermatozoa at different glycerol concentrations and freezing\/thawing rates","volume":"33","author":"Rota","year":"1998","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib51","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1016\/S0093-691X(97)00066-6","article-title":"Effects of equex STM paste on viability of frozen-thawed dog spermatozoa during in vitro incubation at 38\u202f\u00b0C","volume":"47","author":"Rota","year":"1997","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib52","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/S0378-4320(00)00130-5","article-title":"A glimpse at sperm function in vivo: sperm transport and epithelial interaction in the female reproductive tract","volume":"60","author":"Scott","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib53","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.fertnstert.2003.12.039","article-title":"Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization","volume":"82","author":"Seli","year":"2004","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib54","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1053\/tvjl.2002.0704","article-title":"Effect of tris-buffer, egg yolk and glycerol on canine semen freezing","volume":"164","author":"Silva","year":"2002","journal-title":"Vet. J."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib55","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1016\/S0093-691X(02)01130-5","article-title":"Quality of canine semen submitted to single or fractionated glycerol addition during the freezing process","volume":"59","author":"Silva","year":"2003","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib56","doi-asserted-by":"crossref","first-page":"427","DOI":"10.2478\/pjvs-2014-0061","article-title":"Use of biochemical markers to evaluate the quality of fresh and cryopreserved semen from the arctic fox (Vulpes lagopus)","volume":"17","author":"Stasiak","year":"2014","journal-title":"Pol. J. Vet. Sci."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib57","first-page":"224","article-title":"Diuretic effects of medetomidine compared with xylazine in healthy dogs","volume":"73","author":"Talukder","year":"2009","journal-title":"Can. J. Vet. Res.-Rev. Can. Rech. Vet"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib58","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1016\/j.theriogenology.2006.01.022","article-title":"Artificial insemination with frozen semen in dogs: a retrospective study of 10 years using a non-surgical approach","volume":"66","author":"Thomassen","year":"2006","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib59","first-page":"269","article-title":"Gamete physiology and timing of ovulation and fertilization in dogs","volume":"39","author":"Tsutsui","year":"1989","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib60","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1292\/jvms.62.533","article-title":"Effects of orvus ES paste on canine spermatozoal longevity after freezing and thawing","volume":"62","author":"Tsutsui","year":"2000","journal-title":"J. Vet. Med. Sci."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib61","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.anireprosci.2013.10.005","article-title":"Effect of cryopreservation and single layer centrifugation on canine sperm DNA fragmentation assessed by the sperm chromatin dispersion test","volume":"143","author":"Urbano","year":"2013","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib62","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anireprosci.2012.06.003","article-title":"Reproduction in the endangered African wild dog: basic physiology, reproductive suppression and possible benefits of artificial insemination","volume":"133","author":"Van den Berghe","year":"2012","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib63","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.repbio.2014.07.001","article-title":"DNA integrity of fresh and frozen canine epididymal spermatozoa","volume":"14","author":"Varesi","year":"2014","journal-title":"Reprod. Biol."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib64","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1530\/rep.1.01049","article-title":"Within and between breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm","volume":"131","author":"Waterhouse","year":"2006","journal-title":"Reproduction"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib65","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1071\/RD9950871","article-title":"Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function","volume":"7","author":"Watson","year":"1995","journal-title":"Reprod. Fertil. Dev."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib66","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1093\/sysbio\/46.4.622","article-title":"Molecular systematics of the Canidae","volume":"46","author":"Wayne","year":"1997","journal-title":"Syst. Biol."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib67","unstructured":"R. Woodroffe, C. Sillero-Zubiri, Lycaon pictus, The IUCN Red List of Threatened Species, Version 2017-1, www.iucnredlist.org, (Accessed on 12 July 2017)."},{"key":"10.1016\/j.cryobiol.2017.12.095_bib68","series-title":"Department of Reproductive Health and Research, WHO Laboratory Manual for the Examination and Processing of Human Semen","author":"World Health Organization","year":"2010"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib69","author":"WWF INT"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib70","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/S0093-691X(00)00373-3","article-title":"Influence of sugar supplementation of the extender on motility viability and acrosomal integrity of dog spermatozoa during freezing","volume":"54","author":"Yildiz","year":"2000","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2017.12.095_bib71","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1016\/j.theriogenology.2006.01.023","article-title":"Influence of cooling rates and addition of Equex pasta on cooled and frozen-thawed semen of generic gray (Canis lupus) and Mexican gray wolves (C-l. baileyi)","volume":"66","author":"Zindl","year":"2006","journal-title":"Theriogenology"}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224017303747?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224017303747?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T22:23:06Z","timestamp":1762122186000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224017303747"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2]]},"references-count":71,"alternative-id":["S0011224017303747"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2017.12.095","relation":{},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2018,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A two-step dilution tris-egg yolk extender containing Equex STM significantly improves sperm cryopreservation in the African wild dog (Lycaon pictus)","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2017.12.095","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}