{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T02:00:03Z","timestamp":1792634403907,"version":"4.5.0"},"reference-count":70,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00c3\u00bcrkiye Bilimsel ve Teknolojik Ara\u00c5\u009ftirma Kurumu","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2020,8]]},"DOI":"10.1016\/j.cryobiol.2020.03.008","type":"journal-article","created":{"date-parts":[[2020,4,4]],"date-time":"2020-04-04T01:53:43Z","timestamp":1585965223000},"page":"157-163","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":48,"special_numbering":"C","title":["The synergistic effect of trehalose and low concentrations of cryoprotectants can improve post-thaw ram sperm parameters"],"prefix":"10.1016","volume":"95","author":[{"given":"Ali Erdem","family":"\u00d6zt\u00fcrk","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa","family":"Bodu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa Numan","family":"Bucak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vahit","family":"A\u011f\u0131r","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ay\u015fe","family":"\u00d6zcan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazan","family":"Keskin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u0131nar","family":"\u0130li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tohid Rezaei","family":"Topraggaleh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00fcmeyra","family":"Sidal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuri","family":"Ba\u015fp\u0131nar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u015e\u00fckr\u00fc","family":"Dursun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cryobiol.2020.03.008_bib1","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1016\/S0093-691X(02)00653-2","article-title":"Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations","volume":"57","author":"Aisen","year":"2002","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib2","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.anireprosci.2005.07.001","article-title":"Amides as cryoprotectants for freezing stallion semen: a review","volume":"89","author":"Alvarenga","year":"2005","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib3","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/0011-2240(87)90036-8","article-title":"Modes of interaction of cryoprotectants with membrane phospholipids during freezing","volume":"24","author":"Anchordoguy","year":"1987","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib4","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/0011-2240(86)90002-7","article-title":"Osmotic stress as a factor in the detrimental effect of glycerol on human platelets","volume":"23","author":"Armitage","year":"1986","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib5","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/0011-2240(91)90041-L","article-title":"Effect of Ca on the cryoprotective action of trehalose","volume":"28","author":"Bakas","year":"1991","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib6","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1089\/rej.2014.1656","article-title":"Cryoprotectant toxicity: facts, issues, and questions","volume":"18","author":"Best","year":"2015","journal-title":"Rejuvenation Res."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib7","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.smallrumres.2006.12.001","article-title":"Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen","volume":"73","author":"Bucak","year":"2007","journal-title":"Small Rumin. Res."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib8","first-page":"67","article-title":"Cryoprotectants and cryoprotective effect in cryopreservation of gamete cells","volume":"54","author":"Bucak","year":"2007","journal-title":"Vet. J. Ankara Univ."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib9","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.cryobiol.2013.04.007","article-title":"Raffinose and hypotaurine improve the post-thawed Merino ram sperm parameters","volume":"67","author":"Bucak","year":"2013","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib10","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.smallrumres.2008.11.011","article-title":"Effect of antioxidants on microscopic semen parameters, lipid peroxidation and antioxidant activities in Angora goat semen following cryopreservation","volume":"81","author":"Bucak","year":"2009","journal-title":"Small Rumin. Res."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib11","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1016\/j.theriogenology.2006.12.004","article-title":"The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen: microscopic and oxidative stress parameters after freeze\u2013thawing process","volume":"67","author":"Bucak","year":"2007","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib12","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.anireprosci.2014.09.006","article-title":"Cryopreservation of bull sperm: effects of extender supplemented with different cryoprotectants and antioxidants on sperm motility, antioxidant capacity and fertility results","volume":"150","author":"B\u00fcy\u00fckleblebici","year":"2014","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib13","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.theriogenology.2011.02.013","article-title":"Effects of antioxidants and duration of pre-freezing equilibration on frozen-thawed ram semen","volume":"76","author":"C\u00e2mara","year":"2011","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib14","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1093\/humrep\/17.5.1274","article-title":"Reliability of the comet assay in cryopreserved human sperm","volume":"17","author":"Duty","year":"2002","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib15","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1016\/0011-2240(84)90053-1","article-title":"The effect of glycerol concentration and cooling velocity on cryosurvival of ram spermatozoa frozen in straws","volume":"21","author":"Fiser","year":"1984","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib16","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1093\/ilar.41.4.187","article-title":"Mechanisms of cryoinjury in living cells","volume":"41","author":"Gao","year":"2000","journal-title":"ILAR J."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib17","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1095\/biolreprod53.2.276","article-title":"Viability assessment of mammalian sperm using SYBR-14 and propidium iodide","volume":"53","author":"Garner","year":"1995","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib18","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.1095\/biolreprod57.6.1401","article-title":"Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa","volume":"57","author":"Garner","year":"1997","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib19","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1016\/S0093-691X(02)01177-9","article-title":"Influence of extender, temperature, and addition of glycerol on post-thaw sperm parameters in ram semen","volume":"59","author":"Gil","year":"2003","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib20","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1095\/biolreprod53.5.985","article-title":"Effect of cryoprotectant solutes on water permeability of human spermatozoa","volume":"53","author":"Gilmore","year":"1995","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib21","doi-asserted-by":"crossref","first-page":"1700","DOI":"10.1016\/j.theriogenology.2012.05.002","article-title":"Effects of reactive oxygen species on sperm function","volume":"78","author":"Guthrie","year":"2012","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib22","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/0011-2240(92)90004-L","article-title":"Cryopreservation of poultry sperm: the enigma of glycerol","volume":"29","author":"Hammerstedt","year":"1992","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib23","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1002\/j.1939-4640.1990.tb01583.x","article-title":"Cryopreservation of mammalian sperm: what we ask them to survive","volume":"11","author":"Hammerstedt","year":"1990","journal-title":"J. Androl."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib24","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1111\/j.1439-0272.2009.01002.x","article-title":"TUNEL assay and SCSA determine different aspects of sperm DNA damage","volume":"42","author":"Henkel","year":"2010","journal-title":"Andrologia"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib25","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/aja.2010.76","article-title":"Leukocytes and oxidative stress: dilemma for sperm function and male fertility","volume":"13","author":"Henkel","year":"2011","journal-title":"Asian J. Androl."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib26","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0378-4320(00)00152-4","article-title":"Basic aspects of frozen storage of semen","volume":"62","author":"Holt","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib27","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.imr.2016.12.001","article-title":"Cryopreservation and its clinical applications","volume":"6","author":"Jang","year":"2017","journal-title":"Integr. Med. Res."},{"issue":"4","key":"10.1016\/j.cryobiol.2020.03.008_bib28","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/S0093-691X(03)00050-5","article-title":"Subtle membrane changes in cryopreserved bull semen in relation with sperm viability, chromatin structure, and field fertility","volume":"60","author":"Januskauskas","year":"2003","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib29","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.cryobiol.2019.06.001","article-title":"Cryopreservation of Bangladeshi ram semen using different diluents and manual freezing techniques","volume":"89","author":"Jha","year":"2019","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib30","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1002\/ar.1092130103","article-title":"Rapid formation of gap- junction like structures induced by glycerol","volume":"213","author":"Kachar","year":"1985","journal-title":"Anat. Rec."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib31","doi-asserted-by":"crossref","first-page":"7617","DOI":"10.1063\/1.472548","article-title":"Condensation of supersaturated vapors of hydrogen bonding molecules: ethylene glycol, propylene glycol, trimethylene glycol, and glycerol","volume":"105","author":"Kane","year":"1996","journal-title":"J. Chem. Phys."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib32","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1006\/cryo.1998.2128","article-title":"Mouse spermatozoa in high concentration of glycerol: chemical toxicity vs osmotic shock at normal and reduced oxygen concentrations","volume":"37","author":"Katkov","year":"1998","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib33","doi-asserted-by":"crossref","first-page":"156","DOI":"10.17555\/jvc.2017.04.34.2.156","article-title":"Effect of ethylene glycol and antioxidant combination on function of frozen-thawed spermatozoa in Korean jeju black","volume":"34","author":"Ko","year":"2017","journal-title":"Bull. J. Vet. Clin."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib34","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1006\/cryo.1993.1005","article-title":"Effect of various cryoprotective agents and membrane stabilizing compounds on bull sperm membrane integrity after cooling and freezing","volume":"30","author":"Leeuw","year":"1993","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib35","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.cryobiol.2016.11.001","article-title":"Optimization of the cryopreservation of dromedary camel semen: cryoprotectants and their concentration and equilibration times","volume":"74","author":"Malo","year":"2017","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib36","doi-asserted-by":"crossref","first-page":"2047","DOI":"10.1016\/j.theriogenology.2006.06.004","article-title":"Comparing ethylene glycol with glycerol for cryopreservation of canine semen in egg-yolk TRIS extenders","volume":"66","author":"Martins-Bessa","year":"2006","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib37","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1046\/j.1439-0531.2001.00248.x","article-title":"Cryopreservation of embryos of farm animals","volume":"36","author":"Massip","year":"2001","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib38","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/0011-2240(77)90175-4","article-title":"The role of intracellular freezing in the Death of cells cooled at supraoptimal rates","volume":"44","author":"Mazur","year":"1977","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib39","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1095\/biolreprod66.5.1485","article-title":"Is intracellular ice formation the cause of Death of mouse sperm frozen at high cooling rates?","volume":"66","author":"Mazur","year":"2002","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib40","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1093\/oxfordjournals.humrep.a136112","article-title":"osmotic and physiological responses zygotes and human oocytes to mono and disccharides","volume":"10","author":"Mcwilliams","year":"1995","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib41","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.jevs.2006.03.003","article-title":"Effect of cooling rate and cryoprotectant on the cryosurvival of equine spermatozoa","volume":"26","author":"Moore","year":"2006","journal-title":"J. Equine Vet. Sci."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib42","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1177\/002215540104900912","article-title":"A silver staining method for single-cell gel assay","volume":"49","author":"Nadin","year":"2001","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib43","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.1095\/biolreprod.102.011445","article-title":"A triple-stain flow cytometric method to assess plasma-and acrosome-membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles","volume":"68","author":"Nagy","year":"2003","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib44","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.cryobiol.2012.12.010","article-title":"Effects of different concentrations of BHT on microscopic and oxidative parameters of Mahabadi goat semen following the freeze-thaw process","volume":"66","author":"Naijian","year":"2013","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib45","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.anireprosci.2016.12.004","article-title":"Ethylene glycol, but not DMSO, could replace glycerol inclusion in soybean lecithin-based extenders in ram sperm cryopreservation","volume":"177","author":"Najafi","year":"2017","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib46","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/BF01279457","article-title":"Reactions of yeast cells to glycerol treatment alterations to membrane structure and glycerol uptake","volume":"92","author":"Niedermeyer","year":"1977","journal-title":"Protoplasma"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib47","doi-asserted-by":"crossref","DOI":"10.1116\/1.4939462","article-title":"Interaction of ice binding proteins with ice, water and ions","volume":"11","author":"Oude Vrielink","year":"2016","journal-title":"Biointerphases"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib48","series-title":"Cryobiology and Cryopreservation of Sperm. Cryopreservation","author":"\u00d6zt\u00fcrk","year":"2019"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib49","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.1095\/biolreprod.103.025627","article-title":"Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa","volume":"71","author":"Paasch","year":"2004","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib50","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.colsurfb.2004.05.003","article-title":"Preservation mechanisms of trehalose in food and biosystems","volume":"40","author":"Patist","year":"2005","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib51","author":"Pena"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib52","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1111\/j.1365-2605.2011.01218.x","article-title":"The role of mitochondria in energy production for human sperm motility","volume":"35","author":"Piomboni","year":"2012","journal-title":"Int. J. Androl."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib53","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1038\/164666a0","article-title":"Revival of spermatozoa after vitrification and dehydration at low temperatures","volume":"164","author":"Polge","year":"1949","journal-title":"Nature"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib54","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1071\/RD14237","article-title":"Aquaporins 7 and 11 in boar spermatozoa: detection, localisation and relationship with sperm quality","volume":"28","author":"Prieto-Mart\u00ednez","year":"2016","journal-title":"Reprod. Fertil. Dev."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib55","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0014-4827(50)90046-2","article-title":"The microstructure of ram spermatozoa","volume":"1","author":"Randall","year":"1950","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib56","first-page":"8","article-title":"The effect of glycerol and a glycerol-containing cryoprotective medium upon the motility of human sperm prior to freezing, and subsequent difficulties in assessing sperm motility following dilution","volume":"7","author":"Robinson","year":"2018","journal-title":"J. Reprod. Biotechnol. Fertil."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib57","doi-asserted-by":"crossref","first-page":"1848","DOI":"10.1016\/j.theriogenology.2005.10.015","article-title":"Comparison between glycerol and ethylene glycol for dog semen cryopreservation","volume":"65","author":"Rota","year":"2006","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib58","first-page":"367","article-title":"Membrane stabilisation during: the role of two natural cryoprotectants","volume":"22","author":"Rudolph","year":"1985","journal-title":"Trehalose and Proline"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib59","first-page":"138","article-title":"Antioxidative effects of cysteamine, hyaluronan and fetuin on post\u2010thaw semen quality, DNA integrity and oxidative stress parameters in the","volume":"47","author":"Sar\u0131\u00f6zkan","year":"2015","journal-title":"Brown Swiss bull. Andrologia"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib60","first-page":"49","article-title":"Effects of cryoprotective additives on intracellular ice","volume":"19","author":"Shimada","year":"1977","journal-title":"Contrib. Inst. Low Temp. Sci."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib61","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.anireprosci.2012.05.014","article-title":"In vitro evaluation of ram sperm frozen with glycerol, ethylene glycol or acetamide","volume":"132","author":"Silva","year":"2012","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib62","first-page":"33","article-title":"Viability and fertility of stallion semen frozen with ethylene glycol and acetamide as a cryogenic agent","volume":"9","author":"Snoeck","year":"2018","journal-title":"Anim. Reprod."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib63","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1006\/cryo.1998.2097","article-title":"Comparison of glicerol, other polyols, trehalose and raffinose to provide a defined cryoprotectant medium for mouse sperm cryopreservation","volume":"37","author":"Storey","year":"1998","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib64","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1016\/j.theriogenology.2011.04.015","article-title":"Comparing ethylene glycol with glycerol for cryopreservation of buffalo bull semen in egg-yolk containing extenders","volume":"76","author":"Swelum","year":"2011","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib65","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.cryobiol.2018.04.008","article-title":"History of cryobiology, with special emphasis in evolution of mouse sperm cryopreservation","volume":"82","author":"Sztein","year":"2018","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib66","doi-asserted-by":"crossref","first-page":"2061","DOI":"10.1093\/humrep\/dep214","article-title":"Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis","volume":"24","author":"Thomson","year":"2009","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib67","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/0011-2240(76)90123-1","article-title":"Optimal conditions for the preservation of mouse lymph node cells in liquid nitrogen using cooling rate techniques","volume":"13","author":"Thorpe","year":"1976","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2020.03.008_bib68","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/S0378-4320(00)00099-3","article-title":"The causes of reduced fertility with cryopreserved semen","volume":"60\u201361","author":"Watson","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib69","unstructured":"P.F. Watson, G.J. Morris, Cold Shock Injury in Animal Cells. In Temperature and Animal Cells. Symposia of the Society for Experimental Biology. 41 Company of Biologists, Cambridge, UK. 311-340. https:\/\/europepmc.org\/article\/med\/3332489."},{"key":"10.1016\/j.cryobiol.2020.03.008_bib70","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1080\/01652176.1997.9694758","article-title":"Fundamentals and recent development in cryopreservation of bull and boar semen","volume":"19","author":"Woelders","year":"1997","journal-title":"Vet. Q."}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224020300390?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224020300390?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T22:23:41Z","timestamp":1762122221000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224020300390"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8]]},"references-count":70,"alternative-id":["S0011224020300390"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2020.03.008","relation":{},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2020,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The synergistic effect of trehalose and low concentrations of cryoprotectants can improve post-thaw ram sperm parameters","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2020.03.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}