{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T21:13:44Z","timestamp":1791580424043,"version":"4.3.4"},"reference-count":38,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100006692","name":"Universit\u00e0 degli Studi di Torino","doi-asserted-by":"publisher","award":["MARN_RILO_19_01"],"award-info":[{"award-number":["MARN_RILO_19_01"]}],"id":[{"id":"10.13039\/501100006692","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2020,8]]},"DOI":"10.1016\/j.cryobiol.2020.06.006","type":"journal-article","created":{"date-parts":[[2020,6,16]],"date-time":"2020-06-16T21:32:29Z","timestamp":1592343149000},"page":"51-59","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Effect of relaxin on cryopreserved beef bull semen characteristics"],"prefix":"10.1016","volume":"95","author":[{"given":"Ahmed R.","family":"Elkhawagah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola A.","family":"Martino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Ricci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Storti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Rumbolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Lange-Consiglio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila","family":"Vincenti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cryobiol.2020.06.006_bib1","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1111\/j.1439-0531.2007.00890.x","article-title":"Effect of antibiotics in extender on bacterial control and spermatozoal quality of cooled buffalo (Bubalus.bubalis) bull semen","volume":"43","author":"Akhter","year":"2008","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib2","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1530\/rep.1.00011","article-title":"Modifications of bull spermatozoa induced by three extenders: biociphos, low density lipoprotein and Triladyl, before, during and after freezing and thawing","volume":"129","author":"Amirat","year":"2005","journal-title":"Reproduction"},{"key":"10.1016\/j.cryobiol.2020.06.006_bib3","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1111\/and.12555","article-title":"Isolation of bacteria in semen and evaluation of antibiotics in extender for cryopreservation of buffalo (Bubalus bubalis) bull spermatozoa","volume":"48","author":"Andrabi","year":"2016","journal-title":"Andrologia"},{"key":"10.1016\/j.cryobiol.2020.06.006_bib4","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1095\/biolreprod66.2.354","article-title":"Sperm apoptosis in fresh and cryopreserved bull semen detected by flow cytometry and its relationship with fertility","volume":"66","author":"Anzar","year":"2002","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib5","doi-asserted-by":"crossref","first-page":"739","DOI":"10.4081\/ijas.2007.s2.739","article-title":"Evaluation of buffalo semen by Trypan blue\/Giemsa staining and related fertility in vitro","volume":"6","author":"Boccia","year":"2007","journal-title":"Ital. J. Anim. Sci."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib6","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.anireprosci.2007.02.001","article-title":"Effects that bovine sperm cryopreservation using two different extenders has on sperm membranes and chromatin","volume":"104","author":"Celeghini","year":"2008","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib7","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.1439-0531.2006.00712.x","article-title":"Assessment of sperm apoptosis in cryopreserved bull semen after swim-up treatment: a flow cytometric study","volume":"42","author":"Chaveiro","year":"2007","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib8","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1093\/humrep\/12.6.1184","article-title":"A simplified quantitative cervical mucus penetration test","volume":"12","author":"Clarke","year":"1997","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib9","first-page":"791","article-title":"Quality and fertility of the frozen-thawed bull semen as affected by the different cryoprotectants and glutathione levels","volume":"7","author":"El-Harairy","year":"2011","journal-title":"J. Am. Sci."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib10","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1111\/rda.12575","article-title":"Effect of relaxin on fertility parameters of frozen-thawed buffalo (Bubalus bubalis) sperm","volume":"50","author":"Elkhawagah","year":"2015","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib11","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1071\/RDv26n1Ab145","article-title":"Effect of relaxin on fertilizing ability of buffalo sperm","volume":"26","author":"Elkhawagah","year":"2013","journal-title":"Reprod. Fertil. Dev."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib12","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1023\/A:1009844109023","article-title":"Sperm chromatin structure assay is useful for fertility assessment","volume":"22","author":"Evenson","year":"2000","journal-title":"Methods Cell Sci."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib13","doi-asserted-by":"crossref","first-page":"474","DOI":"10.2164\/jandrol.110.012625","article-title":"Effect of Relaxin on human sperm functions","volume":"33","author":"Ferlin","year":"2012","journal-title":"J. Androl."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib14","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s12958-015-0021-4","article-title":"Beneficial effects of relaxin on motility characteristics of stored boar spermatozoa","volume":"13","author":"Feugang","year":"2015","journal-title":"Reprod. Biol. Endocrinol."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib15","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1262\/jrd.47.393","article-title":"Evaluation of bull fertility by migration of frozen-thawed and washed sperm in medium containing cervical mucus","volume":"47","author":"Hamano","year":"2001","journal-title":"J. Reprod. Dev."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib16","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1262\/jrd.18038","article-title":"Effect of relaxin on in vitro fertilization (IVF) of porcine oocytes","volume":"52","author":"Han","year":"2006","journal-title":"J. Reprod. Dev."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib17","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/S0093-691X(99)00162-4","article-title":"High bovine blastocyst development in a static in vitro production system using SOFaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins","volume":"52","author":"Holm","year":"1999","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2020.06.006_bib18","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1016\/S0093-691X(01)00456-3","article-title":"Assessment of sperm quality through fluorimetry and sperm chromatin structure assay in relation to field fertility of frozen\u2013thawed semen from Swedish AI bulls","volume":"55","author":"Januskauskas","year":"2001","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2020.06.006_bib19","first-page":"97","article-title":"Relationship between sperm mitochondrial membrane potential, sperm motility, and fertility potential","volume":"4","author":"Kasai","year":"2002","journal-title":"Asian J. Androl."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib20","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.anireprosci.2007.01.002","article-title":"Computer automated motion analysis of crossbred bull spermatozoa and its relationship with in vitro fertility in zona\u2013free hamster oocytes","volume":"104","author":"Kathiravan","year":"2008","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib21","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1292\/jvms.09-0411","article-title":"Effects of recombinant relaxin on in vitro maturation of porcine oocytes","volume":"7","author":"Kim","year":"2010","journal-title":"J. Vet. Med. Sci."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib22","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1046\/j.1365-2605.2003.00409.x","article-title":"Seminal immuno reactive relaxin in domestic animals and its relationship to sperm motility as a possible index for predicting the fertilizing ability of sires","volume":"26","author":"Kohsaka","year":"2003","journal-title":"Int. J. Androl."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib23","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1016\/j.theriogenology.2007.02.010","article-title":"Seasonality affects post\u2013thaw plasma membrane intactness and sperm velocities in spermatozoa from Thai AI swamp buffaloes (Bubalus bubalis)","volume":"67","author":"Koonjaenak","year":"2007","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2020.06.006_bib24","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/S0015-0282(16)48868-0","article-title":"The effect of relaxin on the motility of sperm in freshly thawed human semen","volume":"44","author":"Lessing","year":"1985","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib25","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1084\/jem.182.5.1545","article-title":"Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by over expression of Bcl-2 and Abl","volume":"182","author":"Martin","year":"1995","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib26","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1111\/j.1447-0578.2006.00144.x","article-title":"Effect of relaxin on motility, acrosome reaction and viability of cryopreserved boar spermatozoa","volume":"9","author":"Miah","year":"2006","journal-title":"Reprod. Med. Biol."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib27","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1111\/j.1740-0929.2007.00468.x","article-title":"Effect of relaxin on motility, acrosome reaction, and utilization of glucose in fresh and frozen-thawed bovine spermatozoa","volume":"78","author":"Miah","year":"2007","journal-title":"Anim. Sci. J."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib28","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.anireprosci.2011.03.010","article-title":"Intracellular signaling cascades induced by relaxin in the stimulation of capacitation and acrosome reaction in fresh and frozen-thawed bovine spermatozoa","volume":"125","author":"Miah","year":"2011","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib29","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/j.1447-0578.2007.00198.x","article-title":"Effect of relaxin and IGF-I on capacitation, acrosome reaction, cholesterol efflux and utilization of labeled and unlabeled glucose in porcine spermatozoa","volume":"7","author":"Miah","year":"2008","journal-title":"Reprod. Med. Biol."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib30","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1016\/0093-691X(90)90552-5","article-title":"Assessment of bull fertility using a mucus penetration test and a human chorionic gonadotrophin stimulation test","volume":"34","author":"Murase","year":"1990","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2020.06.006_bib31","first-page":"207","article-title":"Physiological aspects about semen freezing. Investigacion extension production bovina caprina, fac. Med. Vet. Zootecnia, centro ensenanza, univ. Nacl. Autonoma Mexico,tepotzottan, edo Mexico, Mexico","volume":"25","author":"Palacio-Ango","year":"1994","journal-title":"Veterinaria (Mexico)"},{"key":"10.1016\/j.cryobiol.2020.06.006_bib32","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1071\/RD17356","article-title":"Secretome derived from different cell lines in bovine embryo production in vitro","volume":"30","author":"Perrini","year":"2018","journal-title":"Reprod. Fertil. Dev."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib33","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.cryobiol.2008.11.006","article-title":"The influence of cysteine and taurine on microscopic-oxidative stress parameters and fertilizing ability of bull semen following cryopreservation","volume":"58","author":"Sariozkan","year":"2009","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2020.06.006_bib34","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.anireprosci.2007.05.005","article-title":"Evaluation of sperm functional attributes in relation to in vitro sperm\u2013zona pellucida binding ability and cleavage rate in assessing frozen-thawed buffalo (Bubalus bubalis) semen quality","volume":"106","author":"Selvaraju","year":"2008","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0378-4320(95)01376-B","article-title":"The effect of optimal and suboptimal concentrations of spermon the fertility of fresh and frozen bovine semen and a theoretical model to explain the fertility differences","volume":"39","author":"Shannon","year":"1995","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2020.06.006_bib36","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1016\/j.theriogenology.2007.01.009","article-title":"Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: comparison between two extenders","volume":"67","author":"Stradaioli","year":"2007","journal-title":"Theriogenololy"},{"key":"10.1016\/j.cryobiol.2020.06.006_bib37","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0093-691X(01)00664-1","article-title":"Computer assisted semen analyzers in andrology research and veterinary practice","volume":"57","author":"Verstegen","year":"2002","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2020.06.006_bib38","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/S0378-4320(00)00099-3","article-title":"The causes of reduced fertility with cryopreserved semen","volume":"61","author":"Watson","year":"2000","journal-title":"Anim. Reprod. Sci 60\u2013"}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224019306364?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224019306364?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T23:02:04Z","timestamp":1777417324000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224019306364"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8]]},"references-count":38,"alternative-id":["S0011224019306364"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2020.06.006","relation":{},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2020,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Effect of relaxin on cryopreserved beef bull semen characteristics","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2020.06.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}