{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T13:31:04Z","timestamp":1781789464289,"version":"3.54.5"},"reference-count":11,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003967","name":"Iran National Elites Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003967","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2021,12]]},"DOI":"10.1016\/j.cryobiol.2021.09.008","type":"journal-article","created":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T21:42:37Z","timestamp":1632346957000},"page":"147-149","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"special_numbering":"C","title":["Flow cytometry study of post-thawed bulk spermatozoa: Mito-TEMPO improves cryopreservation performance by controlling apoptosis rate, DNA fragmentation and ROS production"],"prefix":"10.1016","volume":"103","author":[{"given":"S.","family":"Esmaeilkhanian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Asadzadeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Masoudi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cryobiol.2021.09.008_bib1","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1124\/jpet.107.129445","article-title":"Mitochondrial Bax translocation accelerates DNA fragmentation and cell necrosis in a murine model of acetaminophen hepatotoxicity","volume":"324","author":"Bajt","year":"2008","journal-title":"J. Pharmacol. Exp. Therapeut."},{"key":"10.1016\/j.cryobiol.2021.09.008_bib2","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/j.theriogenology.2006.02.022","article-title":"Effect of docosahexaenoic acid and \u03b1-tocopherol enrichment in chicken sperm on semen quality, sperm lipid composition and susceptibility to peroxidation","volume":"66","author":"Cerolini","year":"2006","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2021.09.008_bib3","doi-asserted-by":"crossref","first-page":"26185","DOI":"10.1074\/jbc.M503062200","article-title":"Disruption of fusion results in mitochondrial heterogeneity and dysfunction","volume":"280","author":"Chen","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.cryobiol.2021.09.008_bib4","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1007\/s00204-016-1692-0","article-title":"Mitochondria-targeted antioxidant Mito-Tempo protects against acetaminophen hepatotoxicity","volume":"91","author":"Du","year":"2017","journal-title":"Arch. Toxicol."},{"key":"10.1016\/j.cryobiol.2021.09.008_bib5","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.cryobiol.2014.09.005","article-title":"Inhibition of ROS production through mitochondria-targeted antioxidant and mitochondrial uncoupling increases post-thaw sperm viability in yellow catfish","volume":"69","author":"Fang","year":"2014","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2021.09.008_bib6","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.cryobiol.2017.12.009","article-title":"Mitochondria-targeted antioxidant MitoTEMPO improves the post-thaw sperm quality","volume":"80","author":"Lu","year":"2018","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2021.09.008_bib7","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.cryobiol.2019.10.005","article-title":"Supplementation of chilling storage medium with glutathione protects rooster sperm quality","volume":"92","author":"Masoudi","year":"2020","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2021.09.008_bib8","doi-asserted-by":"crossref","first-page":"106103","DOI":"10.1016\/j.anireprosci.2019.06.015","article-title":"Effects of CoQ10 on the quality of ram sperm during cryopreservation in plant and animal based extenders","volume":"208","author":"Masoudi","year":"2019","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2021.09.008_bib9","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1080\/10715760802582183","article-title":"Antioxidant properties of MitoTEMPOL and its hydroxylamine","volume":"43","author":"Trnka","year":"2009","journal-title":"Free Radic. Res."},{"key":"10.1016\/j.cryobiol.2021.09.008_bib10","doi-asserted-by":"crossref","first-page":"1406","DOI":"10.1016\/j.freeradbiomed.2007.12.036","article-title":"A mitochondria-targeted nitroxide is reduced to its hydroxylamine by ubiquinol in mitochondria","volume":"44","author":"Trnka","year":"2008","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.cryobiol.2021.09.008_bib11","first-page":"1","article-title":"Mito-TEMPO improves development competence by reducing superoxide in preimplantation porcine embryos","volume":"8","author":"Yang","year":"2018","journal-title":"Sci. Rep."}],"updated-by":[{"DOI":"10.1016\/j.cryobiol.2022.11.238","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000}}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224021001541?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224021001541?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T00:44:46Z","timestamp":1757897086000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224021001541"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12]]},"references-count":11,"alternative-id":["S0011224021001541"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2021.09.008","relation":{"erratum":[{"id-type":"doi","id":"10.1016\/j.cryobiol.2022.11.238","asserted-by":"object"}]},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2021,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Flow cytometry study of post-thawed bulk spermatozoa: Mito-TEMPO improves cryopreservation performance by controlling apoptosis rate, DNA fragmentation and ROS production","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2021.09.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}