{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:36:34Z","timestamp":1790199394425,"version":"4.1.0"},"reference-count":77,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2023,3]]},"DOI":"10.1016\/j.cryobiol.2023.01.001","type":"journal-article","created":{"date-parts":[[2023,1,2]],"date-time":"2023-01-02T18:46:51Z","timestamp":1672685211000},"page":"24-35","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"special_numbering":"C","title":["Germplasm cryopreservation in bulls: Effects of gonadal tissue type, cryoprotectant agent, and freezing-thawing rates on sperm quality parameters"],"prefix":"10.1016","volume":"110","author":[{"given":"N.","family":"Chicaiza-Cabezas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Garcia-Herreros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6905-1672","authenticated-orcid":false,"given":"P.M.","family":"Aponte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cryobiol.2023.01.001_bib1","article-title":"Concentraci\u00f3n y morfolog\u00eda de los espermatozoides epididimarios obtenidos de toros postmortem","volume":"52","author":"Albers","year":"2011","journal-title":"Rev. Fac. Cienc. Vet."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib2","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1016\/S0093-691X(00)00470-2","article-title":"Fertilizing potential in vitro of semen from young beef bulls containing a high or low percentage of sperm with a proximal droplet","volume":"54","author":"Amann","year":"2000","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib3","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1007\/s10815-007-9177-y","article-title":"Mammalian sperm chromatin structure and assessment of DNA fragmentation","volume":"24","author":"Andrabi","year":"2007","journal-title":"J. Assist. Reprod. Genet."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib4","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/1477-7827-5-36","article-title":"Spermatozoal sensitive biomarkers to defective protaminosis and fragmented DNA","volume":"5","author":"Angelopoulou","year":"2007","journal-title":"Reprod. Biol. Endocrinol. RBE."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib6","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1093\/humrep\/deac015","article-title":"Sperm acquire epididymis-derived proteins through epididymosomes","volume":"37","author":"Barrachina","year":"2022","journal-title":"Hum. Reprod. Oxf. Engl."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib7","doi-asserted-by":"crossref","first-page":"29","DOI":"10.3109\/01485019008987591","article-title":"Ultrastructural changes in membranes and acrosome of human sperm during cryopreservation","volume":"25","author":"Barthelemy","year":"1990","journal-title":"Arch. Androl."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib8","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1093\/humrep\/deg199","article-title":"Evaluation of the one-step eosin-nigrosin staining technique for human sperm vitality assessment","volume":"18","author":"Bj\u00f6rndahl","year":"2003","journal-title":"Hum. Reprod. Oxf. Engl."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib9","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1146\/annurev-animal-030117-014603","article-title":"Biobanking genetic material for agricultural animal species","volume":"6","author":"Blackburn","year":"2018","journal-title":"Annu. Rev. Anim. Biosci."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib10","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1002\/jez.1400630212","article-title":"Size of explant and volume of medium in tissue cultures","volume":"63","author":"Buchsbaum","year":"1932","journal-title":"J. Exp. Zool."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib11","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S0749-0720(15)30336-4","article-title":"Common causes of infertility in the bull","volume":"13","author":"Camp","year":"1997","journal-title":"Vet. Clin. Food Anim. Pract."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib12","first-page":"1","volume":"vol. 48","author":"Campbell","year":"1956"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib13","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.anireprosci.2007.02.001","article-title":"Effects that bovine sperm cryopreservation using two different extenders has on sperm membranes and chromatin","volume":"104","author":"Celeghini","year":"2008","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib14","doi-asserted-by":"crossref","first-page":"1270","DOI":"10.3168\/jds.S0022-0302(85)80956-5","article-title":"Environmental and genetic sources of variation for seminal quality in mature Holstein bulls","volume":"68","author":"Chandler","year":"1985","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib15","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.theriogenology.2005.05.005","article-title":"Genetic sperm defects","volume":"64","author":"Chenoweth","year":"2005","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib16","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1038\/aja.2010.97","article-title":"The epididymis, cytoplasmic droplets and male fertility","volume":"13","author":"Cooper","year":"2011","journal-title":"Asian J. Androl."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib17","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/S0093-691X(97)00029-0","article-title":"Sperm membrane functional integrity and response of frozen-thawed bovine spermatozoa during the hypoosmotic swelling test incubation at varying temperatures","volume":"47","author":"Correa","year":"1997","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib18","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1590\/S0102-09352010000400001","article-title":"Induction of the acrosome reaction test to in vitro estimate embryo production in Nelore cattle","volume":"62","author":"Costa","year":"2010","journal-title":"Arq. Bras. Med. Vet. Zootec."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib19","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1007\/978-1-59745-362-2_21","article-title":"Cryopreservation of mammalian semen","volume":"368","author":"Curry","year":"2007","journal-title":"Methods Mol. Biol. Clifton NJ"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib21","doi-asserted-by":"crossref","first-page":"R27","DOI":"10.1530\/REP-13-0420","article-title":"New insights into epididymal function in relation to sperm maturation","volume":"147","author":"Dacheux","year":"2014","journal-title":"Reprod. Camb. Engl."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib22","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/j.1439-0272.1988.tb01058.x","article-title":"Correlation between defects in chromatin condensation of human spermatozoa stained by aniline blue and semen characteristics","volume":"20","author":"Dadoune","year":"1988","journal-title":"Andrologia"},{"issue":"Suppl 2","key":"10.1016\/j.cryobiol.2023.01.001_bib23","article-title":"Conservation genetics, precision conservation, and de-extinction","volume":"47","author":"Desalle","year":"2017","journal-title":"Hastings Cent. Rep."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib24","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.2527\/jas1974.3961136x","article-title":"Evaluation of epididymal sperm by the cannulation technique and the effects of in vivo storage in angus bulls","volume":"39","author":"Deutscher","year":"1974","journal-title":"J. Anim. Sci."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib25","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1002\/mrd.1080380116","article-title":"Computerized analysis of the motility parameters of hamster spermatozoa during maturation","volume":"38","author":"Devi","year":"1994","journal-title":"Mol. Reprod. Dev."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib26","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1002\/cyto.a.10015","article-title":"Toluidine blue test for sperm DNA integrity and elaboration of image cytometry algorithm","volume":"52","author":"Erenpreisa","year":"2003","journal-title":"Cytometry Part J. Int. Soc. Anal. Cytol."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib27","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1002\/j.1939-4640.1996.tb01785.x","article-title":"Media and dilution procedures tested to minimize handling effects on human, rabbit, and bull sperm for computer-assisted sperm analysis (CASA)","volume":"17","author":"Farrell","year":"1996","journal-title":"J. Androl."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib28","first-page":"123","article-title":"Viability of fresh and frozen bull sperm compared by two staining techniques","volume":"2","author":"Felipe-P\u00e9rez","year":"2008","journal-title":"Acta Vet. Bras."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib29","first-page":"1313","article-title":"[Effect of epidermal growth factor (EGF) on mouse sperm capacitation]","volume":"45","author":"Furuya","year":"1993","journal-title":"Nippon. Sanka Fujinka Gakkai Zasshi"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib30","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1016\/S0015-0282(16)56295-5","article-title":"Effect of epidermal growth factor on human sperm capacitation","volume":"60","author":"Furuya","year":"1993","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib31","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1111\/andr.12320","article-title":"Molecular changes and signaling events occurring in sperm during epididymal maturation","volume":"5","author":"Gervasi","year":"2017","journal-title":"Andrology"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib32","first-page":"80","article-title":"Post-thaw evaluation of cryopreserved bull semen extended in four different semen extenders","volume":"11","author":"Ghareeb","year":"2017","journal-title":"Aust. J. Basic Appl. Sci."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib33","doi-asserted-by":"crossref","first-page":"1704","DOI":"10.3390\/ani10091704","article-title":"Genomic evaluation of primiparous high-producing dairy cows: inbreeding effects on genotypic and phenotypic production\u2013reproductive traits","volume":"10","author":"Guti\u00e9rrez-Reinoso","year":"2020","journal-title":"Animals"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib34","first-page":"1","article-title":"A review of inbreeding depression in dairy cattle: current status, emerging control strategies, and future prospects","author":"Guti\u00e9rrez-Reinoso","year":"2022","journal-title":"J. Dairy Res."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib35","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1111\/j.1439-0272.1984.tb00230.x","article-title":"How to increase the motility of spermatozoa from the epididymis of bulls and alloplastic spermatoceles in minipigs","volume":"16","author":"Hamm","year":"1984","journal-title":"Andrologia"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib36","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1111\/andr.12641","article-title":"Dark side of the epididymis: tails of sperm maturation","volume":"7","author":"Hermo","year":"2019","journal-title":"Andrology"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib37","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1093\/biolre\/ioy045","article-title":"Sperm acrosome reaction: its site and role in fertilization","volume":"99","author":"Hirohashi","year":"2018","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib38","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1038\/aja.2012.81","article-title":"Mechanism of sperm capacitation and the acrosome reaction: role of protein kinases","volume":"14","author":"Ickowicz","year":"2012","journal-title":"Asian J. Androl."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib39","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1002\/j.1939-4640.2002.tb02276.x","article-title":"Development of a cryopreservation protocol for type A spermatogonia","volume":"23","author":"Izadyar","year":"2002","journal-title":"J. Androl."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib40","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/0011-2240(86)90014-3","article-title":"Pharmacology of DMSO","volume":"23","author":"Jacob","year":"1986","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib41","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1530\/jrf.0.0700219","article-title":"Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics","volume":"70","author":"Jeyendran","year":"1984","journal-title":"J. Reprod. Fertil."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib42","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0749-0720(15)30339-X","article-title":"The significance to bull fertility of morphologically abnormal sperm","volume":"13","author":"Johnson","year":"1997","journal-title":"Vet. Clin. North Am. Food Anim. Pract."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib43","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1530\/jrf.0.0770435","article-title":"Preparation of a plasma membrane-rich fraction from rat spermatozoa","volume":"77","author":"Jones","year":"1986","journal-title":"J. Reprod. Fertil."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib44","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/0142-9612(96)85562-1","article-title":"Long-term storage of tissues by cryopreservation: critical issues","volume":"17","author":"Karlsson","year":"1996","journal-title":"Biomaterials"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib45","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1080\/09712119.2015.1129343","article-title":"Evaluation of bull semen for fertility-associated protein, in vitro characters and fertility","volume":"45","author":"Karunakaran","year":"2017","journal-title":"J. Appl. Anim. Res."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib46","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.1093\/humrep\/del508","article-title":"Methods of cryopreservation of testicular tissue with viable spermatogonia in pre-pubertal boys undergoing gonadotoxic cancer treatment","volume":"22","author":"Keros","year":"2007","journal-title":"Hum. Reprod. Oxf. Engl."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib47","doi-asserted-by":"crossref","first-page":"S49","DOI":"10.1016\/j.ijvsm.2017.11.001","article-title":"Evaluation of bull spermatozoa during and after cryopreservation: structural and ultrastructural insights","volume":"6","author":"Khalil","year":"2017","journal-title":"Int. J. Vet. Sci. Med."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib48","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1016\/S0093-691X(98)00166-6","article-title":"Cryopreservation and ensuing in vitro fertilization ability of boar spermatozoa from epididymides stored at 4 degrees C","volume":"50","author":"Kikuchi","year":"1998","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib49","doi-asserted-by":"crossref","first-page":"23","DOI":"10.5653\/cerm.2013.40.1.23","article-title":"The utility of sperm DNA damage assay using toluidine blue and aniline blue staining in routine semen analysis","volume":"40","author":"Kim","year":"2013","journal-title":"Clin. Exp. Reprod. Med."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib50","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1002\/j.1939-4640.2004.tb02798.x","article-title":"Immunofluorescence reveals ubiquitination of retained distal cytoplasmic droplets on ejaculated porcine spermatozoa","volume":"25","author":"Kuster","year":"2004","journal-title":"J. Androl."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib51","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/0014-5793(94)80422-2","article-title":"Epidermal growth factor induces acrosomal exocytosis in bovine sperm","volume":"339","author":"Lax","year":"1994","journal-title":"FEBS Lett."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib52","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anireprosci.2016.04.013","article-title":"Cryopreservation of bull semen: evolution from egg yolk based to soybean based extenders","volume":"172","author":"Layek","year":"2016","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib53","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1093\/biolre\/iox096","article-title":"Formation and function of sperm tail structures in association with sperm motility defects","volume":"97","author":"Lehti","year":"2017","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib54","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/0011-2240(77)90165-1","article-title":"Water transport in a cluster of closely packed erythrocytes at subzero temperatures","volume":"14","author":"Levin","year":"1977","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib55","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.fertnstert.2010.10.007","article-title":"Selection of viable spermatozoa from testicular biopsies: a comparative study between pentoxifylline and hypoosmotic swelling test","volume":"95","author":"Mangoli","year":"2011","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib56","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S1355-0306(06)71591-X","article-title":"The use of phosphate buffered saline for the recovery of cells and spermatozoa from swabs","volume":"46","author":"Martin","year":"2006","journal-title":"Sci. Justice J. Forensic Sci. Soc."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib57","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.theriogenology.2004.03.003","article-title":"Decay of sperm obtained from epididymes of wild ruminants depending on postmortem time","volume":"63","author":"Martinez-Pastor","year":"2005","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib58","first-page":"277","article-title":"The use of epididymal spermatozoa in assisted reproduction","volume":"53","author":"McLachlan","year":"1998","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib59","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1530\/jrf.0.0990071","article-title":"Effects of cryopreservation on the human sperm acrosome and its response to A23187","volume":"99","author":"McLaughlin","year":"1993","journal-title":"J. Reprod. Fertil."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib60","doi-asserted-by":"crossref","first-page":"619","DOI":"10.2164\/jandrol.05018","article-title":"The motility of epididymal or testicular spermatozoa does not directly affect IVF\/ICSI pregnancy outcomes","volume":"26","author":"Moghadam","year":"2005","journal-title":"J. Androl."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib61","doi-asserted-by":"crossref","first-page":"2006","DOI":"10.1016\/j.jprot.2010.06.005","article-title":"Proteomics of cauda epididymal fluid from mature Holstein bulls","volume":"73","author":"Moura","year":"2010","journal-title":"J. Proteonomics"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib62","doi-asserted-by":"crossref","first-page":"911","DOI":"10.5713\/ajas.15.0002R","article-title":"African indigenous cattle: unique genetic resources in a rapidly changing world","volume":"28","author":"Mwai","year":"2015","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib63","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1093\/humrep\/17.3.704","article-title":"The effects of cryopreservation on sperm morphology, motility and mitochondrial function","volume":"17","author":"O'Connell","year":"2002","journal-title":"Hum. Reprod. Oxf. Engl."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib64","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1093\/humupd\/dmw029","article-title":"Cryopreservation of testicular tissue or testicular cell suspensions: a pivotal step in fertility preservation","volume":"22","author":"Onofre","year":"2016","journal-title":"Hum. Reprod. Update"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib65","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1016\/S2095-3119(16)61489-2","article-title":"Effect of sucrose on cryopreservation of pig spermatogonial stem cells","volume":"16","author":"Pan","year":"2017","journal-title":"J. Integr. Agric."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib66","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/978-1-59745-362-2_3","article-title":"Principles of cryopreservation","volume":"368","author":"Pegg","year":"2007","journal-title":"Methods Mol. Biol. Clifton NJ"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib67","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1071\/RD15399","article-title":"Biobanking genetic resources: challenges and implementation at the USDA national animal germplasm program","volume":"28","author":"Purdy","year":"2015","journal-title":"Reprod. Fertil. Dev."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib68","series-title":"Knobil Neills Physiol. Reprod","first-page":"1071","article-title":"CHAPTER 22 - The Epididymis","author":"Robaire","year":"2006"},{"key":"10.1016\/j.cryobiol.2023.01.001_bib69","doi-asserted-by":"crossref","first-page":"1458","DOI":"10.3168\/jds.S0022-0302(87)80169-8","article-title":"Effects of extender and insemination dose on postthaw quality and fertility of bovine sperm","volume":"70","author":"Schenk","year":"1987","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib70","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/S2305-0500(13)60132-3","article-title":"Acrosome membrane integrity and cryocapacitation are related to cholesterol content of bull spermatozoa","volume":"2","author":"Srivastava","year":"2013","journal-title":"Asian Pac. J. Reprod."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib71","doi-asserted-by":"crossref","first-page":"s27","DOI":"10.1017\/S1751731118000435","article-title":"Review: spermatogenesis in the bull","volume":"12","author":"Staub","year":"2018","journal-title":"Anim. Int. J. Anim. Biosci."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib72","doi-asserted-by":"crossref","DOI":"10.1093\/conphys\/cow069","article-title":"Effects of post-mortem storage conditions of bovine epididymides on sperm characteristics: investigating a tool for preservation of sperm from endangered species","volume":"4","author":"Strand","year":"2016","journal-title":"Conserv. Physiol."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib73","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S0378-4320(00)00231-1","article-title":"The use of in vitro fertilization techniques to investigate the fertilizing ability of bovine sperm with proximal cytoplasmic droplets","volume":"65","author":"Thundathil","year":"2001","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib74","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/S0378-4320(96)01586-2","article-title":"Assessment of the acrosomal status of membrane-intact ram spermatozoa after freezing and thawing, by simultaneous lectin\/Hoechst 33258 staining","volume":"45","author":"Valc\u00e1rcel","year":"1997","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib75","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0378-4320(94)90061-2","article-title":"Fertility of long-term-stored boar semen: influence of extender (Androhep and Kiev), storage time and plasma droplets in the semen","volume":"36","author":"Waberski","year":"1994","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib76","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1136\/vr.97.1.12","article-title":"Use of a Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa","volume":"97","author":"Watson","year":"1975","journal-title":"Vet. Rec."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib77","first-page":"402","article-title":"Cryopreservation of adult bovine testicular tissue for spermatogonia enrichment","volume":"32","author":"Wu","year":"2011","journal-title":"Cryo Lett."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib78","doi-asserted-by":"crossref","first-page":"1876","DOI":"10.1074\/mcp.R800005-MCP200","article-title":"Sperm chromatin","volume":"7","author":"Wu","year":"2008","journal-title":"Mol. Cell. Proteomics MCP."},{"key":"10.1016\/j.cryobiol.2023.01.001_bib79","doi-asserted-by":"crossref","first-page":"2402","DOI":"10.1093\/humrep\/den272","article-title":"Long-term spermatogonial survival in cryopreserved and xenografted immature human testicular tissue","volume":"23","author":"Wyns","year":"2008","journal-title":"Hum. Reprod. Oxf. Engl."}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224023000019?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224023000019?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T00:46:41Z","timestamp":1757897201000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224023000019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3]]},"references-count":77,"alternative-id":["S0011224023000019"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2023.01.001","relation":{},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2023,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Germplasm cryopreservation in bulls: Effects of gonadal tissue type, cryoprotectant agent, and freezing-thawing rates on sperm quality parameters","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2023.01.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}