{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T19:29:53Z","timestamp":1793042993257,"version":"4.5.0"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,6,28]],"date-time":"2025-06-28T00:00:00Z","timestamp":1751068800000},"content-version":"am","delay-in-days":300,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2024,9]]},"DOI":"10.1016\/j.cryobiol.2024.104926","type":"journal-article","created":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T04:13:47Z","timestamp":1719548027000},"page":"104926","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Polyethylene glycol and caspase inhibitor emricasan alleviate cold injury in primary rat hepatocytes"],"prefix":"10.1016","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-9248-8550","authenticated-orcid":false,"given":"Huyun","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley W.","family":"Ellis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia T.","family":"Dinicu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammadreza","family":"Mojoudi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin T.","family":"Wilks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2373-232X","authenticated-orcid":false,"given":"Shannon N.","family":"Tessier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3647-3665","authenticated-orcid":false,"given":"Mehmet","family":"Toner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Korkut","family":"Uygun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2600-7900","authenticated-orcid":false,"given":"Basak E.","family":"Uygun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.cryobiol.2024.104926_bib12","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1038\/nbt.3889","article-title":"The promise of organ and tissue preservation to transform medicine","volume":"35","author":"Giwa","year":"2017","journal-title":"Nat. Biotechnol."},{"issue":"Suppl 2","key":"10.1016\/j.cryobiol.2024.104926_bib23","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1111\/ajt.16978","article-title":"OPTN\/SRTR 2020 annual data report: liver","author":"Kwong","year":"2022","journal-title":"Am. J. Transplant."},{"key":"10.1016\/j.cryobiol.2024.104926_bib25","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.cryobiol.2015.12.001","article-title":"The grand challenges of organ banking: proceedings from the first global summit on complex tissue cryopreservation","volume":"72","author":"Lewis","year":"2016","journal-title":"Cryobiology"},{"issue":"4","key":"10.1016\/j.cryobiol.2024.104926_bib3","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1097\/00007890-198804000-00001","article-title":"Principles of solid-organ preservation by cold storage","volume":"45","author":"Belzer","year":"1988","journal-title":"Transplantation"},{"issue":"6","key":"10.1016\/j.cryobiol.2024.104926_bib4","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1097\/00000658-199206000-00004","article-title":"The use of UW solution in clinical transplantation. A 4-year experience","volume":"215","author":"Belzer","year":"1992","journal-title":"Ann. Surg."},{"issue":"6","key":"10.1016\/j.cryobiol.2024.104926_bib48","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/0011-2240(86)90056-8","article-title":"Development of a cold storage solution for pancreas preservation","volume":"23","author":"Wahlberg","year":"1986","journal-title":"Cryobiology"},{"issue":"3","key":"10.1016\/j.cryobiol.2024.104926_bib31","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1159\/000499610","article-title":"Organ preservation into the 2020s: the era of dynamic intervention","volume":"46","author":"Petrenko","year":"2019","journal-title":"Transfus. Med. Hemotherapy"},{"issue":"2","key":"10.1016\/j.cryobiol.2024.104926_bib40","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1111\/ajt.13062","article-title":"UW solution: still the \"gold standard\" for liver transplantation","volume":"15","author":"Stewart","year":"2015","journal-title":"Am. J. Transplant."},{"issue":"1","key":"10.1016\/j.cryobiol.2024.104926_bib30","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1111\/j.1478-3231.2006.01390.x","article-title":"The response of the hepatocyte to ischemia","volume":"27","author":"Massip\u2010Salcedo","year":"2007","journal-title":"Liver Int."},{"key":"10.1016\/j.cryobiol.2024.104926_bib5","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1038\/nm.3588","article-title":"Supercooling enables long-term transplantation survival following 4 days of liver preservation","volume":"20","author":"Berendsen","year":"2014","journal-title":"Nat. Med."},{"key":"10.1016\/j.cryobiol.2024.104926_bib10","doi-asserted-by":"crossref","first-page":"2024","DOI":"10.1038\/s41596-020-0319-3","article-title":"Subzero non-frozen preservation of human livers in the supercooled state","volume":"15","author":"de Vries","year":"2020","journal-title":"Nat. Protoc."},{"issue":"3","key":"10.1016\/j.cryobiol.2024.104926_bib38","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1007\/s10439-022-03064-2","article-title":"Cryopreservation of whole rat livers by vitrification and nanowarming","volume":"51","author":"Sharma","year":"2023","journal-title":"Ann. Biomed. Eng."},{"key":"10.1016\/j.cryobiol.2024.104926_bib41","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.cryobiol.2017.12.036","article-title":"Partial freezing: a nature-inspired strategy for organ banking","volume":"81","author":"Tessier","year":"2018","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2024.104926_bib42","doi-asserted-by":"crossref","first-page":"4008","DOI":"10.1038\/s41467-022-31490-2","article-title":"Partial freezing of rat livers extends preservation time by 5-fold","volume":"13","author":"Tessier","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.cryobiol.2024.104926_bib45","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.cryobiol.2020.10.083","article-title":"Human liver preservation by supercooling: an update","volume":"97","author":"Uygun","year":"2020","journal-title":"Cryobiology"},{"issue":"1","key":"10.1016\/j.cryobiol.2024.104926_bib1","doi-asserted-by":"crossref","first-page":"59","DOI":"10.3727\/000000003783985160","article-title":"Induction of necrosis and DNA fragmentation during hypothermic preservation of hepatocytes in UW, HTK, and Celsior solutions","volume":"12","author":"Abrahamse","year":"2003","journal-title":"Cell Transplant."},{"issue":"1","key":"10.1016\/j.cryobiol.2024.104926_bib32","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3727\/096368911X580509","article-title":"Improvement of the cold storage of isolated human hepatocytes","volume":"21","author":"Pless","year":"2012","journal-title":"Cell Transplant."},{"key":"10.1016\/j.cryobiol.2024.104926_bib36","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1096\/fasebj.13.1.155","article-title":"Cold-induced apoptosis in cultured hepatocytes and liver endothelial cells: mediation by reactive oxygen species","volume":"13","author":"Rauen","year":"1999","journal-title":"Faseb. J."},{"issue":"8","key":"10.1016\/j.cryobiol.2024.104926_bib47","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1016\/S0891-5849(01)00670-0","article-title":"Cold-induced apoptosis in isolated rat hepatocytes: protective role of glutathione","volume":"31","author":"Vairetti","year":"2001","journal-title":"Free Radic. Biol. Med."},{"issue":"3","key":"10.1016\/j.cryobiol.2024.104926_bib16","first-page":"108","article-title":"The effects of cold preservation solutions supplemented with UDCA and \u03b1-lipoic acid on the viability and function of isolated human hepatocytes","volume":"10","author":"Hossein Aghdaie","year":"2019","journal-title":"Inter. J. Organ Trans. Med."},{"issue":"1","key":"10.1016\/j.cryobiol.2024.104926_bib17","first-page":"15","article-title":"Effects of different cold preservation solutions on the functions of cultured isolated human hepatocytes","volume":"11","author":"Hossein Aghdaie","year":"2020","journal-title":"Inter. J. Organ Trans. Med."},{"issue":"3","key":"10.1016\/j.cryobiol.2024.104926_bib19","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1053\/jhep.2002.31869","article-title":"Cold-induced apoptosis of rat liver cells in University of Wisconsin solution: the central role of chelatable iron","volume":"35","author":"Kerkweg","year":"2002","journal-title":"Hepatology"},{"issue":"1","key":"10.1016\/j.cryobiol.2024.104926_bib27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0011-2240(91)90002-6","article-title":"Effect of polyethylene glycol on lipid peroxidation in cold-stored rat hepatocytes","volume":"28","author":"Mack","year":"1991","journal-title":"Cryobiology"},{"issue":"1","key":"10.1016\/j.cryobiol.2024.104926_bib33","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.cryobiol.2015.04.010","article-title":"Polyethylene glycol protects primary hepatocytes during supercooling preservation","volume":"71","author":"Puts","year":"2015","journal-title":"Cryobiology"},{"issue":"1","key":"10.1016\/j.cryobiol.2024.104926_bib34","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.cryobiol.2006.11.008","article-title":"Iron-dependent vs. iron-independent cold-induced injury to cultured rat hepatocytes: a comparative study in physiological media and organ preservation solutions","volume":"54","author":"Rauen","year":"2007","journal-title":"Cryobiology"},{"issue":"12","key":"10.1016\/j.cryobiol.2024.104926_bib35","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.1016\/j.freeradbiomed.2003.09.018","article-title":"Cold-induced apoptosis of hepatocytes: mitochondrial permeability transition triggered by nonmitochondrial chelatable iron","volume":"35","author":"Rauen","year":"2003","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.cryobiol.2024.104926_bib39","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1006\/cryo.1995.1039","article-title":"Effects of hypothermia on the function, membrane integrity, and cytoskeletal structure of hepatocytes","volume":"32","author":"Stefanovich","year":"1995","journal-title":"Cryobiology"},{"issue":"7","key":"10.1016\/j.cryobiol.2024.104926_bib44","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0069334","article-title":"Supercooling as a viable non-freezing cell preservation method of rat hepatocytes","volume":"8","author":"Usta","year":"2013","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.cryobiol.2024.104926_bib15","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1097\/00007890-199901150-00032","article-title":"Subzero nonfreezing storage of isolated rat hepatocytes in University of Wisconsin solution","volume":"67","author":"Hiraki","year":"1999","journal-title":"Transplantation"},{"issue":"1","key":"10.1016\/j.cryobiol.2024.104926_bib37","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S1665-2681(19)31812-5","article-title":"Subzero nonfreezing storage of rat hepatocytes using modified University of Wisconsin solution (mUW) and 1, 4-butanediol. I-effects on cellular metabolites during cold storage","volume":"8","author":"Rodr\u00edguez","year":"2009","journal-title":"Ann. Hepatol."},{"issue":"13","key":"10.1016\/j.cryobiol.2024.104926_bib6","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1096\/fasebj.10.13.8940293","article-title":"Effect of extracellular matrix topology on cell structure, function, and physiological responsiveness: hepatocytes cultured in a sandwich configuration","volume":"10","author":"Berthiaume","year":"1996","journal-title":"Faseb. J."},{"issue":"5","key":"10.1016\/j.cryobiol.2024.104926_bib43","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1001\/jama.1989.03420050061039","article-title":"Extended preservation of human liver grafts with UW solution","volume":"261","author":"Todo","year":"1989","journal-title":"JAMA"},{"issue":"8","key":"10.1016\/j.cryobiol.2024.104926_bib46","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1016\/S0891-5849(01)00670-0","article-title":"Cold-induced apoptosis in isolated rat hepatocytes: protective role of glutathione","volume":"31","author":"Vairetti","year":"2001","journal-title":"Free Radic. Biol. Med."},{"issue":"11","key":"10.1016\/j.cryobiol.2024.104926_bib9","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.3748\/wjg.v19.i11.1683","article-title":"Molecular mechanisms of liver ischemia reperfusion injury: insights from transgenic knockout models","volume":"19","author":"Datta","year":"2013","journal-title":"World J. Gastroenterol."},{"key":"10.1016\/j.cryobiol.2024.104926_bib14","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1038\/s41575-021-00549-8","article-title":"Liver ischaemia\u2013reperfusion injury: a new understanding of the role of innate immunity","volume":"19","author":"Hirao","year":"2022","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"issue":"5","key":"10.1016\/j.cryobiol.2024.104926_bib11","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1073\/pnas.81.5.1411","article-title":"Hepatocyte proliferation in vitro: its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix","volume":"81","author":"Enat","year":"1984","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"2","key":"10.1016\/j.cryobiol.2024.104926_bib21","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1006\/cryo.1995.1017","article-title":"Evidence for two modes of hypothermia damage in five cell lines","volume":"32","author":"Kruuv","year":"1995","journal-title":"Cryobiology"},{"issue":"3","key":"10.1016\/j.cryobiol.2024.104926_bib22","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1006\/cryo.1993.1030","article-title":"Further evidence for two modes of hypothermia damage","volume":"30","author":"Kruuv","year":"1993","journal-title":"Cryobiology"},{"issue":"3","key":"10.1016\/j.cryobiol.2024.104926_bib20","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/S0006-291X(03)00618-1","article-title":"Mitochondrial permeability transition: a common pathway to necrosis and apoptosis","volume":"304","author":"Kim","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.cryobiol.2024.104926_bib24","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1023\/A:1005419617371","article-title":"Mitochondrial dysfunction in the pathogenesis of necrotic and apoptotic cell death","volume":"31","author":"Lemasters","year":"1999","journal-title":"J. Bioenerg. Biomembr."},{"key":"10.1016\/j.cryobiol.2024.104926_bib29","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1016\/0011-2240(89)90077-1","article-title":"Hypothermic preservation of hepatocytes. I. Role of cell swelling","volume":"26","author":"Marsha","year":"1989","journal-title":"Cryobiology"},{"issue":"3","key":"10.1016\/j.cryobiol.2024.104926_bib13","doi-asserted-by":"crossref","first-page":"351","DOI":"10.4161\/auto.5.3.7650","article-title":"Participation of autophagy in the initiation of graft dysfunction after rat liver transplantation","volume":"5","author":"Gotoh","year":"2009","journal-title":"Autophagy"},{"key":"10.1016\/j.cryobiol.2024.104926_bib26","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s13105-021-00846-5","article-title":"Estrogen alleviates hepatocyte necroptosis depending on GPER in hepatic ischemia reperfusion injury","volume":"78","author":"Li","year":"2002","journal-title":"J. Physiol. Biochem."},{"issue":"3","key":"10.1016\/j.cryobiol.2024.104926_bib28","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1152\/physrev.00061.2009","article-title":"Hepatocyte death: a clear and present danger","volume":"90","author":"Malhi","year":"2010","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.cryobiol.2024.104926_bib49","doi-asserted-by":"crossref","first-page":"e996","DOI":"10.1038\/cddis.2013.499","article-title":"Molecular mechanisms of hepatic apoptosis","volume":"5","author":"Wang","year":"2014","journal-title":"Cell Death Dis."},{"key":"10.1016\/j.cryobiol.2024.104926_bib2","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1038\/s42003-021-02297-6","article-title":"Hepatic resistance to cold ferroptosis in a mammalian hibernator Syrian hamster depends on effective storage of diet-derived \u03b1-tocopherol","volume":"4","author":"Anegawa","year":"2021","journal-title":"Commun. Biol."},{"issue":"12","key":"10.1016\/j.cryobiol.2024.104926_bib7","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1089\/ars.2012.5014","article-title":"Iron and reactive oxygen species: friends or foes of cancer cells?","volume":"20","author":"Bystrom","year":"2014","journal-title":"Antioxidants Redox Signal."},{"key":"10.1016\/j.cryobiol.2024.104926_bib8","article-title":"Characteristics and biomarkers of ferroptosis","volume":"9","author":"Chen","year":"2021","journal-title":"Front. Cell Dev. Biol."}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224024000816?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224024000816?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T05:31:23Z","timestamp":1725255083000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224024000816"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9]]},"references-count":48,"alternative-id":["S0011224024000816"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2024.104926","relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-3669876\/v1","asserted-by":"object"}]},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2024,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Polyethylene glycol and caspase inhibitor emricasan alleviate cold injury in primary rat hepatocytes","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2024.104926","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Society for Cryobiology. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"104926"}}