{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T06:07:26Z","timestamp":1778652446901,"version":"3.51.4"},"reference-count":84,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,12,1]],"date-time":"2024-12-01T00:00:00Z","timestamp":1733011200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100004202","name":"Ondokuz Mayis \u00dcniversitesi","doi-asserted-by":"publisher","award":["1904.21.029"],"award-info":[{"award-number":["1904.21.029"]}],"id":[{"id":"10.13039\/501100004202","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019673","name":"Bilimsel Ara\u015ft\u0131rma Projeleri Birimi, \u0130stanbul Teknik \u00dcniversitesi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100019673","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2024,12]]},"DOI":"10.1016\/j.cryobiol.2024.104982","type":"journal-article","created":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T10:27:12Z","timestamp":1729852032000},"page":"104982","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Investigation of the efficacy of different cryoprotectants in the freezing of testicular tissue and epididymal sperm: Spermatological parameters, tissue viability and PARP-1 gene expression"],"prefix":"10.1016","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0666-5359","authenticated-orcid":false,"given":"Cumali","family":"Kaya","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Burcu","family":"Esin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melih","family":"Akar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cansu","family":"Can","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mesut","family":"\u00c7evik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.cryobiol.2024.104982_bib1","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.theriogenology.2009.08.004","article-title":"Cryopreservation of immature porcine testis tissue to maintain its developmental potential after xenografting into recipient mice","volume":"73","author":"Abrishami","year":"2010","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1477-7827-7-143","article-title":"Potential biological role of poly (ADP-ribose) polymerase (PARP) in male gametes","volume":"7","author":"Agarwal","year":"2009","journal-title":"Reprod. Biol. Endocrinol."},{"key":"10.1016\/j.cryobiol.2024.104982_bib3","first-page":"16","volume":"vol. 7","author":"Akal","year":"2021"},{"key":"10.1016\/j.cryobiol.2024.104982_bib4","doi-asserted-by":"crossref","first-page":"3811","DOI":"10.1093\/nar\/gkz120","article-title":"Poly (ADP-ribosyl) ation by PARP1: reaction mechanism and regulatory proteins","volume":"47","author":"Alemasova","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.cryobiol.2024.104982_bib5","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.bpc.2007.09.004","article-title":"Protein precipitation and denaturation by dimethyl sulfoxide","volume":"131","author":"Arakawa","year":"2007","journal-title":"Biophys. Chem."},{"key":"10.1016\/j.cryobiol.2024.104982_bib6","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1016\/j.fertnstert.2012.02.010","article-title":"Orthotopic grafting of cryopreserved prepubertal testicular tissue: in search of a simple yet effective cryopreservation protocol","volume":"97","author":"Baert","year":"2012","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2024.104982_bib7","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1586\/17434440.5.3.359","article-title":"Cryopreservation and vitrification: recent advances in fertility preservation technologies","volume":"5","author":"Bagchi","year":"2008","journal-title":"Expet Rev. Med. Dev."},{"key":"10.1016\/j.cryobiol.2024.104982_bib8","article-title":"Morphometric features and chromatin condensation abnormalities evaluated by toluidine blue staining in bull spermatozoa","volume":"22","author":"Beletti","year":"2017","journal-title":"J. Morphol. Sci."},{"key":"10.1016\/j.cryobiol.2024.104982_bib9","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.theriogenology.2003.10.016","article-title":"Comparison between the toluidine blue stain and the Feulgen reaction for evaluation of rabbit sperm chromatin condensation and their relationship with sperm morphology","volume":"62","author":"Beletti","year":"2004","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib10","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1111\/and.12301","article-title":"Lycopene and resveratrol improve post\u2010thaw bull sperm parameters: sperm motility, mitochondrial activity and DNA integrity","volume":"47","author":"Bucak","year":"2015","journal-title":"Andrologia"},{"key":"10.1016\/j.cryobiol.2024.104982_bib11","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.anireprosci.2016.01.009","article-title":"Enrichment and culture of spermatogonia from cryopreserved adult bovine testis tissue","volume":"166","author":"Cai","year":"2016","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2024.104982_bib12","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.theriogenology.2017.11.006","article-title":"Effect of bovine sperm chromatin integrity evaluated using three different methods on in vitro fertility","volume":"107","author":"Castro","year":"2018","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib13","first-page":"1","article-title":"Agentes crioprotetores intracelulares: caracter\u00edsticas e utiliza\u00e7\u00e3o na criopreserva\u00e7\u00e3o de tecido ovariano e o\u00f3citos","volume":"39","author":"Castro","year":"2011","journal-title":"Acta Sci. Vet."},{"key":"10.1016\/j.cryobiol.2024.104982_bib14","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.cryobiol.2020.03.007","article-title":"Cryopreservation effects on canine sperm morphometric variables and ultrastructure: comparison between vitrification and conventional freezing","volume":"95","author":"Cerdeira","year":"2020","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib15","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1111\/j.1439-0272.2009.00981.x","article-title":"Effects of varicocele upon the expression of apoptosis\u2010related proteins","volume":"42","author":"Chang","year":"2010","journal-title":"Andrologia"},{"key":"10.1016\/j.cryobiol.2024.104982_bib16","first-page":"188","article-title":"Evaluation of frozen thawed cauda epididymal sperms and in vitro fertilizing potential of bovine sperm collected from the cauda epididymal","volume":"16","author":"Chaveiro","year":"2015","journal-title":"Iran. J. Vet. Res."},{"key":"10.1016\/j.cryobiol.2024.104982_bib17","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.cryobiol.2023.01.001","article-title":"Germplasm cryopreservation in bulls: effects of gonadal tissue type, cryoprotectant agent, and freezing-thawing rates on sperm quality parameters","volume":"110","author":"Chicaiza-Cabezas","year":"2023","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.33188\/vetheder.1082675","article-title":"Epididimal manda spermas\u0131n\u0131n dondurulmas\u0131nda spermaya kat\u0131lan farkl\u0131 suland\u0131r\u0131c\u0131lar\u0131n spermatolojik parametreler \u00fczerine etkisinin in-vitro de\u011ferlendirilmesi","volume":"94","author":"Demirci","year":"2021","journal-title":"Veteriner Hekimler Derne\u011fi Dergisi"},{"key":"10.1016\/j.cryobiol.2024.104982_bib19","volume":"vol. 28","author":"Esin","year":"2022"},{"key":"10.1016\/j.cryobiol.2024.104982_bib20","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1177\/30.3.6174566","article-title":"Simultaneous measurement by flow cytometry of sperm cell viability and mitochondrial membrane potential related to cell motility","volume":"30","author":"Evenson","year":"1982","journal-title":"J. Histochem. Cytochem."},{"key":"10.1016\/j.cryobiol.2024.104982_bib21","first-page":"375","article-title":"Cryoprotectants: the essential antifreezes to protect life in the frozen state","volume":"25","author":"Fuller","year":"2004","journal-title":"Cryo-Letters"},{"key":"10.1016\/j.cryobiol.2024.104982_bib22","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.theriogenology.2004.09.023","article-title":"Sperm factors related to in vitro and in vivo porcine fertility","volume":"63","author":"Gadea","year":"2005","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib23","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1038\/sj.cdd.4400851","article-title":"Characterization of the necrotic cleavage of poly (ADP-ribose) polymerase (PARP-1): implication of lysosomal proteases","volume":"8","author":"Gobeil","year":"2001","journal-title":"Cell Death Differ."},{"key":"10.1016\/j.cryobiol.2024.104982_bib24","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.theriogenology.2005.11.018","article-title":"Evaluation of epididymal semen quality using the Hamilton\u2013Thorne analyser indicates variation between the two caudae epididymides of the same bull","volume":"66","author":"Goovaerts","year":"2006","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib25","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1002\/mrd.21394","article-title":"Relationships between the dynamics of iatrogenic DNA damage and genomic design in mammalian spermatozoa from eleven species","volume":"78","author":"Gos\u00e1lvez","year":"2011","journal-title":"Mol. Reprod. Dev."},{"key":"10.1016\/j.cryobiol.2024.104982_bib26","doi-asserted-by":"crossref","first-page":"2399","DOI":"10.1016\/j.fertnstert.2011.03.035","article-title":"Cryopreservation of mouse testicular tissue: prospect for harvesting spermatogonial stem cells for fertility preservation","volume":"95","author":"Gouk","year":"2011","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2024.104982_bib27","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/j.theriogenology.2005.05.006","article-title":"Fertility evaluation of frozen\/thawed semen","volume":"64","author":"Graham","year":"2005","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib28","series-title":"Cryopreservation and Intr Ation and Intracytoplasmic Sperm Injection with Oplasmic Sperm Injection with Bovine Epididymal Spermatozoa","author":"Guerrero","year":"2006"},{"key":"10.1016\/j.cryobiol.2024.104982_bib29","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1095\/biolreprod.107.066357","article-title":"Sperm mitochondrial integrity is not required for hyperactivated motility, zona binding, or acrosome reaction in the rhesus macaque","volume":"79","author":"Hung","year":"2008","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2024.104982_bib30","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S1472-6483(10)61710-5","article-title":"Vitrification of mammalian spermatozoa in the absence of cryoprotectants: from past practical difficulties to present success","volume":"6","author":"Isachenko","year":"2003","journal-title":"Reprod. Biomed. Online"},{"key":"10.1016\/j.cryobiol.2024.104982_bib31","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1016\/j.fertnstert.2007.12.079","article-title":"Determination of Poly (ADP-ribose) polymerase (PARP) homologues in human ejaculated sperm and its correlation with sperm maturation","volume":"91","author":"Jha","year":"2009","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2024.104982_bib32","doi-asserted-by":"crossref","first-page":"3245","DOI":"10.1128\/MCB.01921-07","article-title":"DEK is a poly (ADP-ribose) acceptor in apoptosis and mediates resistance to genotoxic stress","volume":"8","author":"Kappes","year":"2008","journal-title":"Mol. Cell Biol."},{"key":"10.1016\/j.cryobiol.2024.104982_bib33","doi-asserted-by":"crossref","first-page":"2524","DOI":"10.1016\/S0006-3495(93)81319-5","article-title":"Nucleation and growth of ice crystals inside cultured hepatocytes during freezing in the presence of dimethyl sulfoxide","volume":"65","author":"Karlsson","year":"1993","journal-title":"Biophys. J."},{"key":"10.1016\/j.cryobiol.2024.104982_bib34","doi-asserted-by":"crossref","first-page":"1676","DOI":"10.1093\/humrep\/deh797","article-title":"Optimizing cryopreservation of human testicular tissue: comparison of protocols with glycerol, propanediol and dimethylsulphoxide as cryoprotectants","volume":"20","author":"Keros","year":"2005","journal-title":"Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2024.104982_bib35","series-title":"The Cytology of the Testis, the Physiology of Reproduction","author":"De Kretser","year":"1988"},{"key":"10.1016\/j.cryobiol.2024.104982_bib36","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1016\/S0093-691X(99)00201-0","article-title":"The effect of cryopreservation on the survivability, viability and motility of epididymal African buffalo (Syncerus caffer) spermatozoa","volume":"52","author":"Lambrechts","year":"1999","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib37","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1111\/rda.13228","article-title":"Effects of five cryoprotectants on proliferation and differentiation\u2010related gene expression of frozen\u2010thawed bovine calf testicular tissue","volume":"53","author":"Li","year":"2018","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2024.104982_bib38","doi-asserted-by":"crossref","DOI":"10.1590\/0103-8478cr20170135","article-title":"Cryopreservation of testicular tissue: an alternative to maintain the reproductive capacity in different animal species","volume":"47","author":"Lima","year":"2017","journal-title":"Ci\u00eancia Rural."},{"key":"10.1016\/j.cryobiol.2024.104982_bib39","doi-asserted-by":"crossref","first-page":"2210","DOI":"10.1016\/j.fertnstert.2008.02.173","article-title":"Evaluation of poly (ADP-ribose) polymerase cleavage (cPARP) in ejaculated human sperm fractions after induction of apoptosis","volume":"91","author":"Mahfouz","year":"2009","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2024.104982_bib40","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1111\/j.1439-0531.2008.01098.x","article-title":"DNA status on thawed semen from fighting bull: a comparison between the SCD and the SCSA tests","volume":"44","author":"Mart\u00ednez\u2010Pastor","year":"2009","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2024.104982_bib41","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.anireprosci.2007.01.018","article-title":"Cryopreservation of epididymal bovine spermatozoa from dead animals and its uses in vitro embryo production","volume":"101","author":"Martins","year":"2007","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2024.104982_bib42","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.cryobiol.2015.06.003","article-title":"Morphological characterization and conservation of bovine spermatogenic cells by refrigeration at 4 C and freezing using different cryoprotective molecules","volume":"71","author":"Martins","year":"2015","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib43","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.theriogenology.2020.12.004","article-title":"Sperm head morphometry and chromatin condensation are in constant change at seminiferous tubules, epididymis, and ductus deferens in bulls","volume":"161","author":"Martins","year":"2021","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib44","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/s00412-005-0344-6","article-title":"Poly (ADP-ribosyl) ation during chromatin remodeling steps in rat spermiogenesis","volume":"114","author":"Meyer-Ficca","year":"2005","journal-title":"Chromosoma"},{"key":"10.1016\/j.cryobiol.2024.104982_bib45","doi-asserted-by":"crossref","first-page":"S241","DOI":"10.1016\/j.fertnstert.2012.07.879","article-title":"Expression of PARP1 in infertile men and correlation with DNA fragmentation index","volume":"98","author":"Mishra","year":"2012","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2024.104982_bib46","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1111\/rda.13490","article-title":"Dog sperm DNA: raw semen evaluation with Toluidine blue stain","volume":"54","author":"Monachesi","year":"2019","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2024.104982_bib47","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.livsci.2010.08.010","article-title":"Effect of cryopreservation on sperm chromatin integrity and fertilizing potential in bovine semen","volume":"136","author":"Mukhopadhyay","year":"2011","journal-title":"Livest. Sci."},{"key":"10.1016\/j.cryobiol.2024.104982_bib48","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1002\/vms3.355","article-title":"Cryopreservation and its effects on motility and gene expression patterns and fertilizing potential of bovine epididymal sperm","volume":"7","author":"Nazari","year":"2021","journal-title":"Vet. Med. Sci."},{"key":"10.1016\/j.cryobiol.2024.104982_bib49","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.theriogenology.2006.08.002","article-title":"Roles of lipid peroxidation and cytoplasmic droplets on in vitro fertilization capacity of sperm collected from bovine epididymides stored at 4 and 34 C","volume":"67","author":"Nichi","year":"2007","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib50","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1111\/rda.12888","article-title":"Evaluation of epididymis storage temperature and cryopreservation conditions for improved mitochondrial membrane potential, membrane integrity, sperm motility and in vitro fertilization in bovine epididymal sperm","volume":"52","author":"Nichi","year":"2017","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2024.104982_bib51","series-title":"Donmu\u015f Sperman\u0131n Saklanmas\u0131 Ve Eritilmesi","author":"Nur","year":"2003"},{"key":"10.1016\/j.cryobiol.2024.104982_bib52","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1093\/humupd\/dml009","article-title":"Protamines and male infertility","volume":"12","author":"Oliva","year":"2006","journal-title":"Hum. Reprod. Update"},{"key":"10.1016\/j.cryobiol.2024.104982_bib53","series-title":"Cryopreservation of Testicular Tissue","author":"Oliveira","year":"2015"},{"key":"10.1016\/j.cryobiol.2024.104982_bib54","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/j.taap.2005.01.060","article-title":"How do male germ cells handle DNA damage?","volume":"207","author":"Olsen","year":"2005","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1016\/j.cryobiol.2024.104982_bib55","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1093\/humupd\/dmw029","article-title":"Cryopreservation of testicular tissue or testicular cell suspensions: a pivotal step in fertility preservation","volume":"22","author":"Onofre","year":"2016","journal-title":"Hum. Reprod. Update"},{"key":"10.1016\/j.cryobiol.2024.104982_bib56","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1177\/1947601910383418","article-title":"Upregulation of poly (ADP-ribose) polymerase-1 (PARP1) in triple-negative breast cancer and other primary human tumor types","volume":"1","author":"Ossovskaya","year":"2010","journal-title":"Genes Cancer"},{"key":"10.1016\/j.cryobiol.2024.104982_bib57","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1002\/j.1939-4640.2001.tb02219.x","article-title":"Relationship of bull fertility to sperm nuclear shape","volume":"22","author":"Ostermeier","year":"2001","journal-title":"J. Androl."},{"key":"10.1016\/j.cryobiol.2024.104982_bib58","doi-asserted-by":"crossref","first-page":"2","DOI":"10.2353\/ajpath.2008.080019","article-title":"Role of the peroxynitrite-poly (ADP-ribose) polymerase pathway in human disease","volume":"173","author":"Pacher","year":"2008","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.cryobiol.2024.104982_bib59","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.anireprosci.2015.08.004","article-title":"Different extenders in the cryopreservation of bovine epididymal spermatozoa","volume":"161","author":"Papa","year":"2015","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2024.104982_bib60","series-title":"PreservAtion of HumAn Oocytes","first-page":"34","article-title":"Principles of cryopreservation","author":"Pegg","year":"2009"},{"key":"10.1016\/j.cryobiol.2024.104982_bib61","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1751-0147-50-41","article-title":"Morphology and head morphometric characters of sperm in Thai native crossbred stallions","volume":"50","author":"Phetudomsinsuk","year":"2008","journal-title":"Acta Vet. Scand."},{"key":"10.1016\/j.cryobiol.2024.104982_bib62","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.theriogenology.2022.07.020","article-title":"Cryopreservation of testicular tissue from the dog (Canis familiaris) and wild boar (Sus scrofa) by slow freezing and vitrification: differences in cryoresistance according to cell type","volume":"190","author":"Picazo","year":"2022","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib63","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0123957","article-title":"Slow freezing, but not vitrification supports complete spermatogenesis in cryopreserved, neonatal sheep testicular xenografts","volume":"10","author":"Pukazhenthi","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.cryobiol.2024.104982_bib64","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1095\/biolreprod66.1.159","article-title":"Characterization and identification of epididymal factors that protect ejaculated bovine sperm during in vitro storage","volume":"66","author":"Reyes-Moreno","year":"2002","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2024.104982_bib65","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1002\/(SICI)1097-0320(19960601)24:2<167::AID-CYTO9>3.0.CO;2-G","article-title":"Bull sperm head morphometry related to abnormal chromatin structure and fertility","volume":"24","author":"Sailer","year":"1996","journal-title":"Cytometry: J. Int. Soc. Anal. Cytol."},{"key":"10.1016\/j.cryobiol.2024.104982_bib66","doi-asserted-by":"crossref","DOI":"10.1155\/2012\/854837","article-title":"Human sperm cryopreservation: update on techniques, effect on DNA integrity, and implications for ART","volume":"2012","author":"Di Santo","year":"2012","journal-title":"Adv. Urol."},{"key":"10.1016\/j.cryobiol.2024.104982_bib67","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1007\/s10815-012-9885-9","article-title":"CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation","volume":"29","author":"Sivanarayana","year":"2012","journal-title":"J. Assist. Reprod. Genet."},{"key":"10.1016\/j.cryobiol.2024.104982_bib68","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.cryobiol.2007.10.176","article-title":"Comet assay of fresh and cryopreserved bull spermatozoa","volume":"56","author":"S\u0142owi\u0144ska","year":"2008","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib69","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.repbio.2018.04.001","article-title":"Sperm chromatin alterations in fertile and subfertile bulls","volume":"18","author":"Souza","year":"2018","journal-title":"Reprod. Biol."},{"key":"10.1016\/j.cryobiol.2024.104982_bib70","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.anireprosci.2019.06.010","article-title":"Nerve growth factor-\u03b2 effects on post-thaw bull semen quality: effects of nerve growth factor-\u03b2 added to extenders for cryopreservation of electro-ejaculated and epididymal bull semen","volume":"207","author":"Stewart","year":"2019","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2024.104982_bib71","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1262\/jrd.2014-140","article-title":"Evaluation of sperm DNA damage in bulls by TUNEL assay as a parameter of semen quality","volume":"61","author":"Takeda","year":"2015","journal-title":"J. Reprod. Dev."},{"key":"10.1016\/j.cryobiol.2024.104982_bib72","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1095\/biolreprod58.3.786","article-title":"Effect of cryopreservation on bovine sperm organelle function and viability as determined by flow cytometry","volume":"58","author":"Thomas","year":"1998","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2024.104982_bib73","series-title":"Gonadal Tissue Cryopreservation in Fertility Preservation","first-page":"141","article-title":"Testicular tissue cryopreservation","author":"Tournaye","year":"2016"},{"key":"10.1016\/j.cryobiol.2024.104982_bib74","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1016\/j.theriogenology.2011.04.025","article-title":"Assessment of freezing procedures for rat immature testicular tissue","volume":"76","author":"Travers","year":"2011","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib75","first-page":"123","article-title":"Sperm kriyoprezervasyon teknikleri ve fertilizasyon ba\u015far\u0131s\u0131ndaki rol\u00fc","volume":"16","author":"Tunal\u0131","year":"2014","journal-title":"Androloji B\u00fclteni Dergisi"},{"key":"10.1016\/j.cryobiol.2024.104982_bib76","first-page":"29","article-title":"Cryopreservation of epididymal and electroejaculated bull semen using liposome-and egg yolkbased extenders","volume":"12","author":"Turner","year":"2020","journal-title":"Clin. Theriogenol."},{"key":"10.1016\/j.cryobiol.2024.104982_bib77","doi-asserted-by":"crossref","first-page":"122","DOI":"10.7124\/bc.000A26","article-title":"Vitrification of rat testicular tissue using biopolymers","volume":"36","author":"Volkova","year":"2020","journal-title":"Biopolym. Cell"},{"key":"10.1016\/j.cryobiol.2024.104982_bib78","doi-asserted-by":"crossref","first-page":"2347","DOI":"10.1101\/gad.11.18.2347","article-title":"PARP is important for genomic stability but dispensable in apoptosis","volume":"11","author":"Wang","year":"1997","journal-title":"Genes Dev."},{"key":"10.1016\/j.cryobiol.2024.104982_bib79","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.cryobiol.2004.09.001","article-title":"Theoretical prediction of \u2018optimal\u2019freezing programmes","volume":"49","author":"Woelders","year":"2004","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib80","first-page":"402","article-title":"Cryopreservation of adult bovine testicular tissue for spermatogonia enrichment","volume":"32","author":"Wu","year":"2011","journal-title":"Cryo-Letters"},{"key":"10.1016\/j.cryobiol.2024.104982_bib81","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1016\/j.theriogenology.2011.10.039","article-title":"Automatic evaluation of ram sperm morphometry","volume":"77","author":"Y\u00e1niz","year":"2012","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2024.104982_bib82","series-title":"Gebze Teknik \u00dcniversitesi","article-title":"\u0130leri baba Ya\u015f\u0131n\u0131n Oksidatif Stres, DNA Fragmantasyonu, Kromatin Yap\u0131 ve PARP proteininin ekspresyon seviyelerine olan Etkisi","author":"Ya\u015far","year":"2022"},{"key":"10.1016\/j.cryobiol.2024.104982_bib83","doi-asserted-by":"crossref","DOI":"10.1111\/and.12743","article-title":"Effects of various cryoprotectants on the quality of frozen\u2013thawed immature bovine (Qinchuan cattle) calf testicular tissue","volume":"49","author":"Zhang","year":"2017","journal-title":"Andrologia"},{"key":"10.1016\/j.cryobiol.2024.104982_bib84","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.cryobiol.2021.05.001","article-title":"Survivable potential of germ cells after trehalose cryopreservation of bovine testicular tissues","volume":"101","author":"Zhu","year":"2021","journal-title":"Cryobiology"}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224024001378?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224024001378?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T05:08:13Z","timestamp":1778648893000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224024001378"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":84,"alternative-id":["S0011224024001378"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2024.104982","relation":{},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Investigation of the efficacy of different cryoprotectants in the freezing of testicular tissue and epididymal sperm: Spermatological parameters, tissue viability and PARP-1 gene expression","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2024.104982","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Society for Cryobiology. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"104982"}}