{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T17:58:59Z","timestamp":1772819939413,"version":"3.50.1"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100013061","name":"Jilin Provincial Scientific and Technological Development Program","doi-asserted-by":"publisher","award":["YDZJ202203CGZH038"],"award-info":[{"award-number":["YDZJ202203CGZH038"]}],"id":[{"id":"10.13039\/501100013061","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010203","name":"Agriculture Research System of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010203","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007710","name":"Jilin Agricultural University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007710","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFD1200401"],"award-info":[{"award-number":["2021YFD1200401"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011798","name":"Ministry of Agriculture and Rural Affairs of the People&apos;s Republic of China","doi-asserted-by":"publisher","award":["CARS-37"],"award-info":[{"award-number":["CARS-37"]}],"id":[{"id":"10.13039\/501100011798","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Cryobiology"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1016\/j.cryobiol.2025.105291","type":"journal-article","created":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T19:37:31Z","timestamp":1755113851000},"page":"105291","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Effects of years of storage (1, 5, and 7 years) on bovine sperm ultrastructure, oxidative status, and fertilizing capacity"],"prefix":"10.1016","volume":"120","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9752-4094","authenticated-orcid":false,"given":"Chang-Guo","family":"Min","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Si-Min","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-Le","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai-Yan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng-Kui","family":"Hou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin-Hai","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Fang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-Yu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"He","family":"Ding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-Fa","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.cryobiol.2025.105291_bib1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5534\/wjmh.2014.32.1.1","article-title":"Effect of oxidative stress on male reproduction","volume":"32","author":"Agarwal","year":"2014","journal-title":"World J Mens Health"},{"key":"10.1016\/j.cryobiol.2025.105291_bib2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1093\/molehr\/gap059","article-title":"On the possible origins of DNA damage in human spermatozoa","volume":"16","author":"Aitken","year":"2010","journal-title":"Mol. Hum. Reprod."},{"key":"10.1016\/j.cryobiol.2025.105291_bib3","doi-asserted-by":"crossref","first-page":"31","DOI":"10.4103\/1008-682X.122203","article-title":"Oxidative stress and male reproductive health","volume":"16","author":"Aitken","year":"2014","journal-title":"Asian J. Androl."},{"key":"10.1016\/j.cryobiol.2025.105291_bib4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1071\/RD15325","article-title":"Causes and consequences of oxidative stress in spermatozoa","volume":"28","author":"Aitken","year":"2016","journal-title":"Reprod. Fertil. Dev."},{"key":"10.1016\/j.cryobiol.2025.105291_bib5","first-page":"354","article-title":"Effects of long-term storage on some spermatological parameters in cryopreserved bull semen","volume":"39","author":"Akyol","year":"2018","journal-title":"Cryo Lett."},{"key":"10.1016\/j.cryobiol.2025.105291_bib6","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1530\/REP-12-0468","article-title":"Cryopreservation increases coating of bull sperm by seminal plasma binder of sperm proteins BSP1, BSP3, and BSP5","volume":"146","author":"Ardon","year":"2013","journal-title":"Reproduction"},{"key":"10.1016\/j.cryobiol.2025.105291_bib7","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1111\/j.1439-0531.2006.00810.x","article-title":"Practical techniques for bovine sperm simultaneous fluorimetric assessment of plasma, acrosomal and mitochondrial membranes","volume":"42","author":"Celeghini","year":"2007","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2025.105291_bib8","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1016\/j.theriogenology.2014.10.015","article-title":"Comparative transcript profiling of gene expression of fresh and frozen-thawed bull sperm","volume":"83","author":"Chen","year":"2015","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2025.105291_bib9","doi-asserted-by":"crossref","first-page":"2122","DOI":"10.1095\/biolreprod.102.012500","article-title":"Caspase-independent exposure of aminophospholipids and tyrosine phosphorylation in bicarbonate responsive human sperm cells","volume":"68","author":"de Vries","year":"2003","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2025.105291_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.molmet.2018.02.006","article-title":"Sperm epigenetics and influence of environmental factors","volume":"14","author":"Donkin","year":"2018","journal-title":"Mol. Metabol."},{"key":"10.1016\/j.cryobiol.2025.105291_bib11","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1080\/01926230701320337","article-title":"Apoptosis: a review of programmed cell death","volume":"35","author":"Elmore","year":"2007","journal-title":"Toxicol. Pathol."},{"key":"10.1016\/j.cryobiol.2025.105291_bib12","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1590\/S1677-55382011000100002","article-title":"Novel concepts in male infertility","volume":"37","author":"Esteves","year":"2011","journal-title":"Int. Braz. J. Urol."},{"key":"10.1016\/j.cryobiol.2025.105291_bib13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10561-019-09797-0","article-title":"Influence of cryopreservation on structure and function of mammalian spermatozoa: an overview","volume":"21","author":"Ezzati","year":"2020","journal-title":"Cell Tissue Bank."},{"key":"10.1016\/j.cryobiol.2025.105291_bib14","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1016\/j.fertnstert.2005.05.015","article-title":"Successful sperm storage for 28 years","volume":"84","author":"Feldschuh","year":"2005","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2025.105291_bib15","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1016\/j.tcb.2013.07.006","article-title":"Evading apoptosis in cancer","volume":"23","author":"Fernald","year":"2013","journal-title":"Trends Cell Biol."},{"key":"10.1016\/j.cryobiol.2025.105291_bib16","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1111\/rda.13409","article-title":"Recent advances in bovine sperm cryopreservation techniques with a focus on sperm post-thaw quality optimization","volume":"54","author":"Gr\u00f6tter","year":"2019","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2025.105291_bib17","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1038\/nature08162","article-title":"Distinctive chromatin in human sperm packages genes for embryo development","volume":"460","author":"Hammoud","year":"2009","journal-title":"Nature"},{"key":"10.1016\/j.cryobiol.2025.105291_bib18","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.rbmo.2018.05.012","article-title":"Sperm cryopreservation: a review on current molecular cryobiology and advanced approaches","volume":"37","author":"Hezavehei","year":"2018","journal-title":"Reprod. Biomed. Online"},{"issue":"Suppl 2","key":"10.1016\/j.cryobiol.2025.105291_bib19","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1111\/j.1439-0531.2011.01862.x","article-title":"Mechanisms of sperm storage in the female reproductive tract: an interspecies comparison","volume":"46","author":"Holt","year":"2011","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2025.105291_bib20","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1530\/jrf.0.0700219","article-title":"Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics","volume":"70","author":"Jeyendran","year":"1984","journal-title":"J. Reprod. Fertil."},{"key":"10.1016\/j.cryobiol.2025.105291_bib21","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/j.theriogenology.2014.10.014","article-title":"Temporal leakage of Cu,Zn superoxide dismutase and loss of two low-molecular-weight forms of glutathione peroxidase-1 from buffalo (Bubalus bubalis) sperm after freezing and thawing","volume":"83","author":"Kar","year":"2015","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2025.105291_bib22","first-page":"198","article-title":"Effect of pre-incubation of cryopreserved sperm with either Kisspeptin or glutathione to mitigate freeze-thaw damage","volume":"48","author":"Kermani","year":"2023","journal-title":"Iran. J. Med. Sci."},{"key":"10.1016\/j.cryobiol.2025.105291_bib23","first-page":"305","article-title":"Gamma-H2AX - a novel biomarker for DNA double-strand breaks","volume":"22","author":"Kuo","year":"2008","journal-title":"In Vivo"},{"key":"10.1016\/j.cryobiol.2025.105291_bib24","first-page":"867","article-title":"[WHO Laboratory Manual for the Examination and Processing of Human Semen: its applicability to andrology laboratories in China]","volume":"16","author":"Lu","year":"2010","journal-title":"Zhonghua Nan ke Xue"},{"key":"10.1016\/j.cryobiol.2025.105291_bib25","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1095\/biolreprod.103.024281","article-title":"Cryopreservation induces an apoptosis-like mechanism in bull sperm","volume":"71","author":"Martin","year":"2004","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2025.105291_bib26","doi-asserted-by":"crossref","first-page":"1583","DOI":"10.1016\/j.theriogenology.2016.05.018","article-title":"Effect of dietary fish oil supplementation on ram semen freeze ability and fertility using soybean lecithin- and egg yolk-based extenders","volume":"86","author":"Masoudi","year":"2016","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2025.105291_bib27","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1016\/j.theriogenology.2006.11.010","article-title":"Evaluation of a cushioned method for centrifugation and processing for freezing boar semen","volume":"67","author":"Mat\u00e1s","year":"2007","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2025.105291_bib28","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S0093-691X(01)00674-4","article-title":"Current status of sperm cryopreservation: why isn't it better?","volume":"57","author":"Medeiros","year":"2002","journal-title":"Theriogenology"},{"key":"10.1016\/j.cryobiol.2025.105291_bib29","doi-asserted-by":"crossref","DOI":"10.1111\/and.13231","article-title":"Medium containing different concentrations of catalase as a strategy for optimising sperm parameters and chromatin in normospermic persons","volume":"51","author":"Mohammadzadeh","year":"2019","journal-title":"Andrologia"},{"key":"10.1016\/j.cryobiol.2025.105291_bib30","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1093\/humupd\/3.5.403","article-title":"A critical review of the physiological importance and analysis of sperm movement in mammals","volume":"3","author":"Mortimer","year":"1997","journal-title":"Hum. Reprod. Update"},{"key":"10.1016\/j.cryobiol.2025.105291_bib31","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1093\/humupd\/dmab046","article-title":"DNA damage in preimplantation embryos and gametes: specification, clinical relevance and repair strategies","volume":"28","author":"Musson","year":"2022","journal-title":"Hum. Reprod. Update"},{"key":"10.1016\/j.cryobiol.2025.105291_bib32","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.1095\/biolreprod.102.011445","article-title":"A triple-stain flow cytometric method to assess plasma- and acrosome-membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles","volume":"68","author":"Nagy","year":"2003","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.cryobiol.2025.105291_bib33","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1111\/j.1439-0531.2008.01211.x","article-title":"Mitochondria in mammalian sperm physiology and pathology: a review","volume":"44","author":"Pe\u00f1a","year":"2009","journal-title":"Reprod. Domest. Anim."},{"key":"10.1016\/j.cryobiol.2025.105291_bib34","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1007\/s10875-007-9071-5","article-title":"Effects of tumour necrosis factor-alpha on human sperm motility and apoptosis","volume":"27","author":"Perdichizzi","year":"2007","journal-title":"J. Clin. Immunol."},{"key":"10.1016\/j.cryobiol.2025.105291_bib35","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.fertnstert.2003.12.039","article-title":"Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization","volume":"82","author":"Seli","year":"2004","journal-title":"Fertil. Steril."},{"key":"10.1016\/j.cryobiol.2025.105291_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2021.101308","article-title":"Ameliorative role of trans-ferulic acid on induced oxidative toxicity of rooster semen by \u03b2-cyfluthrin during low temperature liquid storage","volume":"100","author":"Shayan-Nasr","year":"2021","journal-title":"Poult. Sci."},{"key":"10.1016\/j.cryobiol.2025.105291_bib37","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1002\/jez.1402150210","article-title":"A triple-stain technique for evaluating normal acrosome reactions of human sperm","volume":"215","author":"Talbot","year":"1981","journal-title":"J. Exp. Zool."},{"key":"10.1016\/j.cryobiol.2025.105291_bib38","doi-asserted-by":"crossref","first-page":"268","DOI":"10.3389\/fvets.2019.00268","article-title":"Advances in cryopreservation of bull sperm","volume":"6","author":"Ugur","year":"2019","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.cryobiol.2025.105291_bib39","article-title":"Kinin-enhancing drugs for unexplained subfertility in men","volume":"1996","author":"Vandekerckhove","year":"2000","journal-title":"Cochrane Database Syst. Rev."},{"key":"10.1016\/j.cryobiol.2025.105291_bib40","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/S0378-4320(00)00099-3","article-title":"The causes of reduced fertility with cryopreserved semen","volume":"60\u201361","author":"Watson","year":"2000","journal-title":"Anim. Reprod. Sci."},{"key":"10.1016\/j.cryobiol.2025.105291_bib41","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1006\/cryo.1997.2028","article-title":"Effects of trehalose and sucrose, osmolality of the freezing medium, and cooling rate on viability and intactness of bull sperm after freezing and thawing","volume":"35","author":"Woelders","year":"1997","journal-title":"Cryobiology"},{"key":"10.1016\/j.cryobiol.2025.105291_bib42","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.cryobiol.2014.03.008","article-title":"Effects of glycerol on apoptotic signaling pathways during boar spermatozoa cryopreservation","volume":"68","author":"Zeng","year":"2014","journal-title":"Cryobiology"}],"container-title":["Cryobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224025000975?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0011224025000975?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T11:51:24Z","timestamp":1772797884000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0011224025000975"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":42,"alternative-id":["S0011224025000975"],"URL":"https:\/\/doi.org\/10.1016\/j.cryobiol.2025.105291","relation":{},"ISSN":["0011-2240"],"issn-type":[{"value":"0011-2240","type":"print"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Effects of years of storage (1, 5, and 7 years) on bovine sperm ultrastructure, oxidative status, and fertilizing capacity","name":"articletitle","label":"Article Title"},{"value":"Cryobiology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.cryobiol.2025.105291","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Society for Cryobiology. Published by Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"105291"}}